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Chimeric caspase molecules with potent cell killing activity in apoptosis-resistant cells.

Abstract：

:Cellular defects which prevent apoptotic cell death can result in the generation of hyperproliferative disorders and can prevent the effective treatment of such diseases. The majority of cellular defects which result in apoptosis resistance lie upstream of caspase activation. We have described chimeric caspase molecules consisting of the prodomain of caspase-2 fused to the amino terminus of caspase-3, and which are tagged at the carboxyl terminus with green fluorescent protein (GFP) to allow direct visualisation of transfected cells. Here we show that these chimeric caspase molecules possess potent, rapid cell-killing activity in cell lines which display a range of defects resulting in apoptosis resistance.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Shearwin-Whyatt L,Baliga B,Doumanis J,Kumar S

doi

10.1006/bbrc.2001.4699

subject

Has Abstract

pub_date

2001-04-20 00:00:00

pages

1114-9

issue

5

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(01)94699-6

journal_volume

282

pub_type

杂志文章

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