当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Efficient silkworm expression of human GPCR (nociceptin receptor) by a Bombyx mori bacmid DNA system.

Efficient silkworm expression of human GPCR (nociceptin receptor) by a Bombyx mori bacmid DNA system.

Abstract：

:Guanine nucleotide-binding protein (G protein) coupled receptors (GPCRs) are frequently expressed by a baculovirus expression vector system (BEVS). We recently established a novel BEVS using the bacmid system of Bombyx mori nucleopolyhedrovirus (BmNPV), which is directly applicable for protein expression in silkworms. Here, we report the first example of GPCR expression in silkworms by the simple injection of BmNPV bacmid DNA. Human nociceptin receptor, an inhibitory GPCR, and its fusion protein with inhibitory G protein alpha subunit (G(i)alpha) were both successfully expressed in the fat bodies of silkworm larvae as well as in the BmNPV viral fraction. Its yield was much higher than that from Sf9 cells. The microsomal fractions including the nociceptin receptor fusion, which are easily prepared by only centrifugation steps, exhibited [35S]GTPgammaS-binding activity upon specific stimulation by nociceptin. Therefore, this rapid method is easy-to-use and has a high expression level, and thus will be an important tool for human GPCR production.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Kajikawa M,Sasaki K,Wakimoto Y,Toyooka M,Motohashi T,Shimojima T,Takeda S,Park EY,Maenaka K

doi

10.1016/j.bbrc.2009.05.063

subject

Has Abstract

pub_date

2009-07-31 00:00:00

pages

375-9

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(09)01015-8

journal_volume

385

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Identification of structural domains affecting transactivation potential of Nm23.

    abstract：:The strong transactivation activity of the C-terminal half (amino acids 76-152) of Nm23 was reported previously. Here we examined a structural domain preventing or necessary to its transactivation activity. The C-terminal 1/4 (amino acids 109-152) was sufficient for transactivation, but the C-terminal half with a long...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.6042

    authors： Cho SJ,Lee NS,Jung YS,Lee H,Lee KJ,Kim E,Chae SK

    更新日期：2001-12-07 00:00:00

  • Canonical Wnt signaling maintains the quiescent stage of hepatic stellate cells.

    abstract：:It is well known that hepatic stellate cells (HSC) develop into cells, which are thought to contribute to liver fibrogenesis. Recent data suggest that HSC are progenitor cells with the capacity to differentiate into cells of endothelial and hepatocyte lineages. The present study shows that beta-catenin-dependent canon...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.12.085

    authors： Kordes C,Sawitza I,Häussinger D

    更新日期：2008-02-29 00:00:00

  • Interaction with Ppil3 leads to the cytoplasmic localization of Apoptin in tumor cells.

    abstract：:Apoptin, a small protein encoded by chicken anemia virus (CAV), induces cell death specifically in cancer cells. In normal cells, Apoptin remains in the cytoplasm; whereas in cancerous cells, it migrates into the nucleus and kills the cell. Cellular localization appears to be crucial. Through a yeast two-hybrid screen...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.04.178

    authors： Huo DH,Yi LN,Yang J

    更新日期：2008-07-18 00:00:00

  • Upregulation of lncRNA-NONRATT021203.2 in the dorsal root ganglion contributes to cancer-induced pain via CXCL9 in rats.

    abstract：:Cancer-induced pain (CIP) is a kind of chronic pain that occurs during cancer progression over time. However, the mechanisms are largely unknown, and clinical treatment remains challenging. LncRNAs have been reported to play critical roles in various biological processes, including chronic pain. The aim of our study w...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.01.163

    authors： Sun RM,Wei J,Wang SS,Xu GY,Jiang GQ

    更新日期：2020-04-16 00:00:00

  • The radial glia antibody RC2 recognizes a protein encoded by Nestin.

    abstract：:The RC2 antibody is widely used to label mouse radial glial cells in the developing central nervous system. While the antibody is known to recognize a 295-kDa intermediate filament proximal protein, the gene encoding the RC2 antigen remains to be identified. Here, we present evidences clearly demonstrating that Nestin...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.03.074

    authors： Park D,Xiang AP,Zhang L,Mao FF,Walton NM,Choi SS,Lahn BT

    更新日期：2009-05-08 00:00:00

  • Inhibition of wortmannin activities by amino compounds.

    abstract：:Wortmannin caused normal and strong inhibition on catecholamine secretion from bovine adrenal chromaffin cells and in vitro phosphoinositide 3-kinase activity in NaCl-, Na isethionate-, choline Cl-, Na acetate-, and N-acetyl glycine-based media. However, brief preincubation of wortmannin with the media containing amin...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.09.200

    authors： Isosaki M

    更新日期：2004-11-26 00:00:00

  • Protein synthesis in rabbit reticulocytes: requirements for Met-tRNAf . 40S preinitiation complex formation with AUG-codon and physiological mRNAs.

    abstract：:Under standard conditions, in the presence of GTP, highly purified eIF-2 and Co-eIF-2 factor preparations efficiently stimulated AUG-codon dependent but not physiological mRNA-dependent Met-tRNAf binding to 40S ribosomes. Replacement of GTP by a nonhydrolyzable GTP analog, GMP-PNP, in the above system, gave significan...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(84)91249-x

    authors： Roy R,Nasrin N,Ahmad MF,Gupta NK

    更新日期：1984-08-16 00:00:00

  • NaCl-induced CsRCI2E and CsRCI2F interact with aquaporin CsPIP2;1 to reduce water transport in Camelina sativa L.

    abstract：:Rare cold-inducible 2 (RCI2) proteins are small hydrophobic proteins that are known to be localized in cellular membranes. The function of RCI2 proteins has been reported to be associated with low-temperature, salt, and drought stress tolerances as a membrane potential regulator; however, the specific functions are st...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.03.208

    authors： Kim HS,Park W,Lim HG,Eom S,Lee JH,Carlson JE,Ahn SJ

    更新日期：2019-05-21 00:00:00

  • Deoxynucleoside triphosphate pool changes and UV-induced depression of DNA synthesis.

    abstract：:It has been reported that changes in deoxynucleotide pool levels following exposure to UV might lead to an overestimation of the UV-induced depression in DNA synthesis as analyzed by incorporation of 3H-thymidine (1). We attempted to determine the importance of such pool effects in two ways. First, we examined the gra...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(84)90292-4

    authors： Griffiths TD,Ling SY

    更新日期：1984-09-17 00:00:00

  • Human kallikrein 13 involvement in extracellular matrix degradation.

    abstract：:The human kallikrein family is a group of 15 serine protease genes clustered on chromosome 19q13.4 and shares a high degree of homology. These proteolytic enzymes have diverse physiological functions in many different tissues. Growing evidence suggests that many kallikreins are differentially expressed in cancer and m...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.08.206

    authors： Kapadia C,Ghosh MC,Grass L,Diamandis EP

    更新日期：2004-10-22 00:00:00

  • Sirtuin inhibition attenuates the production of inflammatory cytokines in lipopolysaccharide-stimulated macrophages.

    abstract：:In several inflammatory conditions such as rheumatoid arthritis or sepsis, the regulatory mechanisms of inflammation are inefficient and the excessive inflammatory response leads to damage to the host. Sirtuins are class III histone deacetylases that modulate the activity of several transcription factors that are impl...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.03.088

    authors： Fernandes CA,Fievez L,Neyrinck AM,Delzenne NM,Bureau F,Vanbever R

    更新日期：2012-04-20 00:00:00

  • Interaction of rat lin-10 with brain-enriched F-actin-binding protein, neurabin-II/spinophilin.

    abstract：:We have recently isolated a rat homologue of the Caenorrhabditis elegans lin-10 product. Although rat lin-10 is expressed in the cytosol and membrane fractions of various tissues, it is distributed only in the membrane fraction in brain where it is enriched in the synaptic plasma membrane and postsynaptic density frac...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.8243

    authors： Ide N,Hata Y,Hirao K,Irie M,Deguchi M,Yao I,Satoh A,Wada M,Takahashi K,Nakanishi H,Takai Y

    更新日期：1998-03-06 00:00:00

  • Monoclonal antibodies against beta-galactoside-binding lectin of chick embryo recognizes conformational change of the antigen.

    abstract：:Monoclonal antibodies against chick embryonic beta-galactoside-binding lectin were obtained. One of the monoclonal antibodies was ineffective in Western blotting and seemed to be unable to bind the SDS-denatured lectin. When the native lectin was dotted on a nitrocellulose filter and subjected to denaturation by treat...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(84)80257-0

    authors： Oda Y,Kasai K

    更新日期：1984-09-28 00:00:00

  • Transforming growth factor-beta1 induces matrix metalloproteinase-9 expression in human meningeal cells via ERK and Smad pathways.

    abstract：:Transforming growth factor (TGF)-beta1, a cytokine released into the cerebrospinal fluid (CSF) after intraventricular hemorrhage (IVH), stimulates the expression of the components of the extracellular matrix (ECM), which causes progressive ventricular dilatation by impaired CSF absorption. Matrix metalloproteinase-9 (...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.04.038

    authors： Okamoto T,Takahashi S,Nakamura E,Nagaya K,Hayashi T,Fujieda K

    更新日期：2009-06-12 00:00:00

  • rDLG6: a novel homolog of Drosophila DLG expressed in rat brain.

    abstract：:Drosophila DLG (Discs Large Tumor Suppresser Protein) is a component of septate junctions, and disruption of its gene leads to over growth of imaginal discs. Homologs of Drosophila DLG recently isolated from mammalian tissue have been classified as members of the MAGUK (Membrane Associated GUanylate Kinase) superfamil...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1723

    authors： Inagaki H,Maeda S,Lin KH,Shimizu N,Saito T

    更新日期：1999-11-19 00:00:00

  • The 31-kDa caspase-generated cleavage product of p130Cas antagonizes the action of MyoD during myogenesis.

    abstract：:Myogenesis is regulated by the basic helix-loop-helix (bHLH) myogenic regulatory factor MyoD, which induces muscle-specific gene expression by binding to the E-box sequence as a heterodimer with ubiquitous bHLH E2A (E12/E47) proteins. Here, we report that a 31-kDa caspase-generated cleavage product of Crk-associated s...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.01.085

    authors： Jeong DE,Lee EK,Song WK,Kim W

    更新日期：2014-02-21 00:00:00

  • Biochemical characterization of recombinant Candida albicans mannosyltransferases Mnt1, Mnt2 and Mnt5 reveals new functions in O- and N-mannan biosynthesis.

    abstract：:The cell surface of Candida albicans is enriched with highly glycosylated mannoproteins that are involved in the interaction with host tissues. N- and O-glycosylation are post-translational modifications that initiate in the endoplasmic reticulum, and finalize in the Golgi. The KRE2/MNT1 family encode a set of multifu...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.01.131

    authors： Díaz-Jiménez DF,Mora-Montes HM,Hernández-Cervantes A,Luna-Arias JP,Gow NA,Flores-Carreón A

    更新日期：2012-03-02 00:00:00

  • Tetraphenylborate potentiates the respiratory inhibition by the dopaminergic neurotoxin MPP+ in both electron transport particles and intact mitochondria.

    abstract：:The cytotoxicity of 1-methyl-4-phenylpyridinium (MPP+) is believed to arise as a consequence of its time- and energy-dependent accumulation inside mitochondria, followed by inhibition of electron transport at Complex I of the respiratory chain. Consistent with our proposal that the accumulation of MPP+ represents a pa...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)92672-7

    authors： Sayre LM,Wang F,Hoppel CL

    更新日期：1989-06-15 00:00:00

  • Sodium-dependent, concentrative nucleoside transport in cultured intestinal epithelial cells.

    abstract：:In a simple salts medium, monolayers of IEC-6 intestinal cells achieved concentrations of unmetabolized formycin B (an analog of inosine) about 6-fold higher than in the medium. Rates of formycin B influx were a saturable function of Na+ concentrations in the medium. Although IEC-6 cells possess sites with high affini...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(86)90738-2

    authors： Jakobs ES,Paterson AR

    更新日期：1986-11-14 00:00:00

  • Protein arginine methyltransferase 6 specifically methylates the nonhistone chromatin protein HMGA1a.

    abstract：:The HMGA family proteins HMGA1a and HMGA1b are nuclear nonhistone species implicated in a wide range of cellular processes including inducible gene transcription, modulation of chromosome structure through nucleosome and chromosome remodeling, and neoplastic transformation. HMGA proteins are highly modified, and chang...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.08.179

    authors： Miranda TB,Webb KJ,Edberg DD,Reeves R,Clarke S

    更新日期：2005-10-28 00:00:00

  • Increased cerebrospinal fluid complement C5 levels in major depressive disorder and schizophrenia.

    abstract：:Inflammation has been implicated in a variety of psychiatric disorders. We aimed to determine whether levels of complement C5 protein in the cerebrospinal fluid (CSF), which may reflect activation of the complement system in the brain, are altered in patients with major psychiatric disorders. Additionally, we examined...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.02.131

    authors： Ishii T,Hattori K,Miyakawa T,Watanabe K,Hidese S,Sasayama D,Ota M,Teraishi T,Hori H,Yoshida S,Nunomura A,Nakagome K,Kunugi H

    更新日期：2018-03-04 00:00:00

  • Synthesis of a nonreducible cyclic analog of somatostatin having only growth hormone release inhibiting activity.

    abstract：:A nonreducible cyclic analog of somatostatin (SRIF) was prepared by a combination of solid phase and solution peptide synthesis. The compound, gamma-Abu-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Asp-OH, was tested for its effect on the release of growth hormone, glucagon and insulin in rats. It significantly suppressed ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(76)90208-4

    authors： Garsky VM,Clark DE,Grant NH

    更新日期：1976-12-20 00:00:00

  • Expression and characterization of a rabbit liver cytochrome P450 belonging to P450IIB subfamily with the aid of the baculovirus expression vector system.

    abstract：:Using baculovirus a cDNA for cytochrome P450 (P450F1) belonging to rabbit P450IIB subfamily was expressed in Spodoptera frugiperda cells, where P450F1 was located on electron-dense structures (derived from the endoplasmic reticulum) present in both the cytoplasm and nucleus. Partially purified P450F1 exhibited absorpt...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)91577-y

    authors： Ohta D,Matsu-ura Y,Sato R

    更新日期：1991-03-15 00:00:00

  • SHP2 associates directly with tyrosine phosphorylated p90 (SNT) protein in FGF-stimulated cells.

    abstract：:In a number of cell lines responsive to basic fibroblast growth factor (bFGF), two major tyrosine phosphorylated proteins, of molecular weights around 120kDa and 90kDa, are precipitated along with the tyrosine phosphatase SHP2 from the lysates of stimulated cells. The docker protein Gab-1 represents at least part of t...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.7272

    authors： Ong SH,Lim YP,Low BC,Guy GR

    更新日期：1997-09-08 00:00:00

  • Resveratrol stimulates the proliferation and differentiation of osteoblastic MC3T3-E1 cells.

    abstract：:Nutritional and pharmacological factors are needed to prevent bone loss that occurs with increasing age. The chemical compounds that act on bone metabolism as nutrients in food, however, are poorly understood. The effect of resveratrol, a natural phytoestrogen, on the proliferation and differentiation of osteoblastic ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.9870

    authors： Mizutani K,Ikeda K,Kawai Y,Yamori Y

    更新日期：1998-12-30 00:00:00

  • The enhanced IL-18 production by UVB irradiation requires ROI and AP-1 signaling in human keratinocyte cell line (HaCaT).

    abstract：:Based on our recent observation that enhanced IL-18 expression positively correlates with malignant skin tumors, such as SCC and melanoma, we examined the possible role of UVB, known to be associated with skin cancer development, in the enhancement of IL-18 production using primary human epidermal keratinocytes and hu...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(02)02433-6

    authors： Cho D,Seung Kang J,Hoon Park J,Kim YI,Hahm E,Lee J,Yang Y,Jeon J,Song H,Park H,Kim T,Pang S,Kim CW,Il Hwang Y,Jae Lee W

    更新日期：2002-10-25 00:00:00

  • Induction of the ethanol-inducible form of rabbit liver microsomal cytochrome P-450 by inhibitors of alcohol dehydrogenase.

    abstract：:Cytochrome P-450 LMeb was purified from liver microsomes obtained from rabbits treated with either benzene or imidazole and was shown to have identical N-terminal amino acid sequence as that of cytochrome P-450 LM3a. The amino acid compositions of the proteins were indistinguishable. Quantitation of P-450 LMeb in vari...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(84)91563-8

    authors： Ingelman-Sundberg M,Jörnvall H

    更新日期：1984-10-30 00:00:00

  • Respiratory syncytial virus non-structural protein 1 facilitates virus replication through miR-29a-mediated inhibition of interferon-α receptor.

    abstract：:Human respiratory syncytial virus (RSV) non-structural protein 1 (NS1) has recently been suggested to inhibit type-I interferon (IFN)-dependent immune responses during RSV infection. However, the precise function of RSV NS1 protein in reducing the antiviral effects of IFNs against RSV is poorly understood. The roles o...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.08.142

    authors： Zhang Y,Yang L,Wang H,Zhang G,Sun X

    更新日期：2016-09-23 00:00:00

  • Effect of superoxide and lipid peroxide on cytosolic free calcium concentration in cultured pig aortic endothelial cells.

    abstract：:The effect of reactive oxygen on cytosolic free calcium concentration [( Ca++]i) in pig aortic endothelial cells (ECs) was studied. Linoleate hydroperoxide (LHO) and superoxide radicals generated from xanthine with xanthine oxidase (X-XO) were used as sources of reactive oxygen. [Ca++]i in ECs was measured with quin 2...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(88)80714-9

    authors： Hirosumi J,Ouchi Y,Watanabe M,Kusunoki J,Nakamura T,Orimo H

    更新日期：1988-04-15 00:00:00

  • Disruption of the mu-delta opioid receptor heteromer.

    abstract：:The crystal structure of the mu and kappa opioid receptors has revealed dimeric structural arrangements. Mu-delta receptors heteromers also exist and we have identified discrete cytoplasmic regions in each receptor required for oligomer formation. In the carboxyl tail of the delta receptor we identified three glycine ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.05.023

    authors： O'Dowd BF,Ji X,O'Dowd PB,Nguyen T,George SR

    更新日期：2012-06-15 00:00:00