当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > A novel fluorescent timer based on bicistronic expression strategy in Caenorhabditis elegans.

A novel fluorescent timer based on bicistronic expression strategy in Caenorhabditis elegans.

Abstract：

:Fluorescent timers are useful tools for studying the spatial and temporal cellular or molecular events. Based on the trans-splicing mechanism in Caenorhabditis elegans, we constructed a "fluorescent timer" through bicistronic expression of two fluorescent proteins with different maturation times. When used in vivo, this "timer" changes its color over time and therefore can be used to monitor the activity of the targeted promoters in C. elegans. Using this "timer", we have successfully traced the time-dependent activity of myo-3 promoter which drives expression in body wall muscle and vulval muscle. We found that the myo-3 promoter started to be active about 7 h after egg-laying and sustained its activity in the following hatching process. We have also determined the myo-3 promoter activity during larval development by this "timer". We anticipate that more new "fluorescent timers" with variable time-resolution could be designed by bicistronic expression of different fluorescent protein pairs.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Chen MR,Yang S,Niu WP,Li ZY,Meng LF,Wu ZX

doi

10.1016/j.bbrc.2010.03.143

subject

Has Abstract

pub_date

2010-04-23 00:00:00

pages

82-6

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(10)00618-2

journal_volume

395

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Inhibition of protein tyrosine phosphatases by low-molecular-weight S-nitrosothiols and S-nitrosylated human serum albumin.

    abstract：:The homogeneous recombinant mammalian protein tyrosine phosphatase 1B (PTP1B) and Yersinia protein tyrosine phosphatase (PTPase) are inactivated by a series of low-molecular-weight S-nitrosothiols. These compounds exhibited different inhibitory activities in a time- and concentration-dependent manner with second-order...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.2117

    authors： Xian M,Wang K,Chen X,Hou Y,McGill A,Zhou B,Zhang ZY,Cheng JP,Wang PG

    更新日期：2000-02-16 00:00:00

  • Histone-H1 inhibits translation by reticulocyte lysates with relative mRNA selectivity.

    abstract：:Histone-H1 purified from rat skeletal muscle is a relatively potent inhibitor of peptide chain initiation in a cell free system, the rabbit reticulocyte lysate (50% inhibition at approximately 0.4 microM). H1 does not inhibit formation of the ternary complex nor its attachment to 40S ribosomes; the data are compatible...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(87)80514-4

    authors： O'Leary NE,Cheema IR,Moore K,Mehard WB,Buse MG

    更新日期：1987-04-14 00:00:00

  • Targeting carbonic anhydrase to treat diabetic retinopathy: emerging evidences and encouraging results.

    abstract：:Diabetic retinopathy (DR) is the leading cause of vision loss among working-age populations in developed countries. Current treatment options are limited to tight glycemic, blood pressure control and destructive laser surgery. Carbonic anhydrases (CAs) are a group of enzymes involving in the rapid conversion of carbon...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章,评审

    doi：10.1016/j.bbrc.2009.10.031

    authors： Weiwei Z,Hu R

    更新日期：2009-12-18 00:00:00

  • Induction of neural stem cell-like cells (NSCLCs) from mouse astrocytes by Bmi1.

    abstract：:Recently, Bmi1 was shown to control the proliferation and self-renewal of neural stem cells (NSCs). In this study, we demonstrated the induction of NSC-like cells (NSCLCs) from mouse astrocytes by Bmi1 under NSC culture conditions. These NSCLCs exhibited the morphology and growth properties of NSCs, and expressed NSC ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.04.068

    authors： Moon JH,Yoon BS,Kim B,Park G,Jung HY,Maeng I,Jun EK,Yoo SJ,Kim A,Oh S,Whang KY,Kim H,Kim DW,Kim KD,You S

    更新日期：2008-06-27 00:00:00

  • Detection of soluble P-glycoprotein in culture media and extracellular fluids.

    abstract：:Multidrug resistance (MDR) is a unique phenomenon in cancer patients and is commonly associated with an overexpression of the human MDR gene mdr1, which encodes an energy-dependent Mr 180 kDa membrane bound protein, known as P-glycoprotein. P-glycoprotein serves as a membrane efflux to pump the drugs out of the cancer...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.2211

    authors： Chu TM,Lin TH,Kawinski E

    更新日期：1994-08-30 00:00:00

  • ERRF is essential for Estrogen-Estrogen Receptor alpha signaling pathway in ER positive breast cancer cells.

    abstract：:Estrogen-Estrogen Receptor alpha (ERα) belongs to one of the most important signaling pathways controlling breast tissue development and progression of breast cancer. ERRF was recently identified as a candidate breast cancer associated protein and showed positive association with ERα status in clinical samples and cel...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.04.132

    authors： Luo A,Zhang X

    更新日期：2016-05-27 00:00:00

  • The histone methyltransferase Dot1 is required for DNA damage repair and proper development in Dictyostelium.

    abstract：:Posttranslational histone modifications play an important role in modulating gene expression and chromatin structure. Here we report the identification of histone H3K79 dimethylation in the simple eukaryote Dictyostelium discoideum. We have deleted the D. discoideum Dot1/KMT4 homologue and demonstrate that it is the s...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.12.101

    authors： Müller-Taubenberger A,Bönisch C,Fürbringer M,Wittek F,Hake SB

    更新日期：2011-01-28 00:00:00

  • Ornithine transcarbamylase deficiency in spf and spf-ash mice: genes, mRNAs and mRNA precursors.

    abstract：:Two ornithine transcarbamylase-deficient mice are available, the spf with a variant enzyme and spf-ash with a markedly decreased enzyme protein. Genomic DNA, mRNA and the nuclear precursors for the enzyme in these mutants were analyzed. Southern blot analysis of genomic DNA showed no abnormality in the mutant mice. Bl...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(87)90755-8

    authors： Ohtake A,Takayanagi M,Yamamoto S,Nakajima H,Mori M

    更新日期：1987-08-14 00:00:00

  • Fibroblast growth factor 2 positively regulates expression of activating transcription factor 4 in osteoblasts.

    abstract：:Our previous studies showed that basic fibroblast growth factor 2 (FGF2) null mice display markedly reduced bone mass and bone formation. However, the mechanism by which FGF2 regulates bone mass or bone formation is not fully defined. Activating transcription factor 4 (ATF4), one member of activating transcription fac...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.11.059

    authors： Fei Y,Xiao L,Hurley MM

    更新日期：2010-01-01 00:00:00

  • Phosphorylation of phosphofructokinase by protein kinase C changes the allosteric properties of the enzyme.

    abstract：:The Ca2+- and phospholipid-dependent protein kinase C from rat brain phosphorylates rabbit muscle phosphofructokinase at the same trypsin-labile site as cyclic AMP-dependent protein kinase. However, protein kinase C also effectively phosphorylates one or more separate sites. Incubation of phosphofructokinase in the pr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(85)91975-8

    authors： Hofer HW,Schlatter S,Graefe M

    更新日期：1985-06-28 00:00:00

  • Chemically induced viral resistance in single preimplantation bovine embryos.

    abstract：:Viral mRNA encoding vesicular stomatitis virus glycoprotein sequences was detected and quantitated using a DNA-hybridization dot-blot technique. This assay was employed to determine if the synthetic double-stranded polynucleotide complex of polyriboinosinic-polyribocytidylic acid would elicit viral resistance in vitro...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)81192-1

    authors： Thomson MS,Bird RC,Stringfellow DA,Rossi CR,Lauerman LH

    更新日期：1991-02-28 00:00:00

  • Paradoxical effect of L-arginine: acceleration of endothelial cell senescence.

    abstract：:We have recently shown that inhibition of nitric oxide (NO) synthesis by asymmetrical dimethylarginine (ADMA) accelerated endothelial cell (EC) senescence which was prevented by coincubation with L-arginine; however the effect of long-term treatment of l-arginine alone on senescence of ECs have not been investigated. ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.06.091

    authors： Scalera F,Closs EI,Flick E,Martens-Lobenhoffer J,Boissel JP,Lendeckel U,Heimburg A,Bode-Böger SM

    更新日期：2009-09-04 00:00:00

  • Acute infection of Sindbis virus induces phosphorylation and intracellular translocation of small heat shock protein HSP27 and activation of p38 MAP kinase signaling pathway.

    abstract：:In general, viral infection is supposed to induce stress responses in the host cell. However, very few detailed observations about virus-induced stress responses have been reported. Here we investigated specific stress responses in Vero cells infected with Sindbis virus (SV), a single-stranded RNA virus, acute infecti...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.9724

    authors： Nakatsue T,Katoh I,Nakamura S,Takahashi Y,Ikawa Y,Yoshinaka Y

    更新日期：1998-12-09 00:00:00

  • ATR microspectroscopy with multivariate analysis segregates grades of exfoliative cervical cytology.

    abstract：:Although cervical cancer screening in the UK has led to reductions in the incidence of invasive disease, this programme remains flawed. We set out to examine the potential of infrared (IR) microspectroscopy to allow the profiling of cellular biochemical constituents associated with disease progression. Attenuated tota...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.11.005

    authors： Walsh MJ,Singh MN,Pollock HM,Cooper LJ,German MJ,Stringfellow HF,Fullwood NJ,Paraskevaidis E,Martin-Hirsch PL,Martin FL

    更新日期：2007-01-05 00:00:00

  • Potent inhibition of tau fibrillization with a multivalent ligand.

    abstract：:Small-molecule inhibitors of tau fibrillization are under investigation as tools for interrogating the tau aggregation pathway and as potential therapeutic agents for Alzheimer's disease. Established inhibitors include thiacarbocyanine dyes, which can inhibit recombinant tau fibrillization in the presence of anionic s...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.08.166

    authors： Honson NS,Jensen JR,Darby MV,Kuret J

    更新日期：2007-11-09 00:00:00

  • Reduction of PTP1B by RNAi upregulates the activity of insulin controlled fatty acid synthase promoter.

    abstract：:Metabolic deregulation accompanying type II diabetes is characterized by insulin resistance in peripheral tissues (liver, muscle, and adipose), mediated by impairments in insulin receptor (IR) signaling. Protein tyrosine phosphatase 1B (PTP1B) has been shown to be a negative regulator of IR autophosphorylation and thu...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.02.016

    authors： Xu J,Li L,Qian Z,Hong J,Shen S,Huang W

    更新日期：2005-04-08 00:00:00

  • Overstretching partially alkyne functionalized dsDNA using near infrared optical tweezers.

    abstract：:The force-extension behaviour of synthesized double-stranded DNAs (dsDNAs) designed to have 2.1% or 6.6% of the thymine bases alkyne functionalized was studied using near infrared (NIR) optical tweezers. Measurements were carried out on substrates with and without flurophores covalently attached to the alkyne moiety o...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.01.087

    authors： Raudsepp A,Kent LM,Hall SB,Williams MAK

    更新日期：2018-02-12 00:00:00

  • Regulation of adiponectin receptor R1 and R2 gene expression in adipocytes of C57BL/6 mice.

    abstract：:Adiponectin has gained significant attention as a mediator of insulin sensitivity. Recently, two receptors of this adipocyte-secreted hormone, adiponectin receptor 1 (AdipoR1) and 2 (AdipoR2), have been cloned. To improve our understanding of the regulation of these receptors in adipocytes, AdipoR1 and AdipoR2 mRNA wa...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.02.081

    authors： Blüher M,Fasshauer M,Kralisch S,Schön MR,Krohn K,Paschke R

    更新日期：2005-04-15 00:00:00

  • Nonhistone protein HMG1 removes the transcriptional block caused by left-handed Z-form segment in a supercoiled DNA.

    abstract：:The effect of HMG1 on the transcriptional block caused by left-handed Z-form in a highly negatively supercoiled DNA was examined using a supercoiled plasmid containing a (CG)10 sequence downstream of promoters. The transcription by E. coli RNA polymerase was blocked at the boundary of alternating CG sequence in the Z-...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(88)81227-0

    authors： Waga S,Mizuno S,Yoshida M

    更新日期：1988-05-31 00:00:00

  • Membrane association of leucyl-tRNA synthetase during leucine starvation in Escherichia coli.

    abstract：:The influence of leucine starvation on the subcellular location of leucyl-tRNA synthetase in Escherichia coli was examined in a leucine auxotrophic strain by sucrose density sedimentation analysis. Analysis of the subcellular distribution of leucyl-tRNA synthetase activity revealed that during unrestricted growth, the...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.1119

    authors： Williamson RM

    更新日期：1993-02-15 00:00:00

  • miR-410-3p promotes prostate cancer progression via regulating PTEN/AKT/mTOR signaling pathway.

    abstract：:Prostate cancer has become one of commonest urologic tumors in male. In recent years, miRNAs are continually attracting attentions of researchers for their special regulatory function in human cancers. Previous study has revealed that miR-410 acts as a biomarker for the diagnosis of prostate cancer. Whereas, the speci...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.06.176

    authors： Zhang Y,Zhang D,Lv J,Wang S,Zhang Q

    更新日期：2018-09-18 00:00:00

  • Role of cholesterol in ligand binding and G-protein coupling of serotonin1A receptors solubilized from bovine hippocampus.

    abstract：:The serotonin(1A) (5-HT(1A)) receptor is an important member of the superfamily of seven transmembrane domain G-protein-coupled receptors. We report here that solubilization of the hippocampal 5-HT(1A) receptor by the zwitterionic detergent CHAPS is accompanied by loss of membrane cholesterol which results in a reduct...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.12.102

    authors： Chattopadhyay A,Jafurulla M,Kalipatnapu S,Pucadyil TJ,Harikumar KG

    更新日期：2005-02-25 00:00:00

  • Improved gene expression by a U3-based retroviral vector.

    abstract：:To improve the expression of the genes transduced by retroviral vectors, we have constructed a U3-based retroviral vector and evaluated its effect on the expression of an insert from the internal promoter. The unique feature of the vector is that the transduced gene is inserted at the U3 region of the 3' long terminal...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(92)91197-x

    authors： Chen BF,Hsieh CL,Chen DS,Hwang LH

    更新日期：1992-04-15 00:00:00

  • Oleanolic acid prevents cartilage degeneration in diabetic mice via PPARγ associated mitochondrial stabilization.

    abstract：:Hyperglycemia-induced cartilage degeneration induces osteoarthritis (OA). Since oleanolic acid (OLA) have several pharmacological effects such as anti-inflammatory, anti-oxidant, we hypothesized it possesses protection against high glucose injured cartilage. We now report that OLA decreased type X collagen and reverse...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.06.127

    authors： Kang X,Yang Z,Sheng J,Liu JB,Xie QY,Zheng W,Chen K

    更新日期：2017-08-26 00:00:00

  • Anabolic function of the type II isozyme of hexokinase in hepatic lipid synthesis.

    abstract：:The mRNA encoding the Type II isozyme of hexokinase was markedly elevated in livers of transgenic mice overexpressing the transcriptionally active nuclear form of sterol regulatory element binding protein-1a (nSREBP-1a), but not in transgenic mice overexpressing the nSREBP-1c or nSREBP-2 isoforms. Cellulose acetate el...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.2527

    authors： Sebastian S,Horton JD,Wilson JE

    更新日期：2000-04-21 00:00:00

  • Expression of peroxisome proliferator activated receptor mRNA in normal and tumorigenic rodent mammary glands.

    abstract：:The peroxisome proliferator activated receptors (PPARs) alpha, beta/delta, and gamma are novel nuclear hormone receptors activated by long chain fatty acids and synthetic ligands and which regulate lipid metabolism. Recent studies have detected PPARgamma mRNA in human mammary tumor cell lines. The current study examin...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.9858

    authors： Gimble JM,Pighetti GM,Lerner MR,Wu X,Lightfoot SA,Brackett DJ,Darcy K,Hollingsworth AB

    更新日期：1998-12-30 00:00:00

  • Blood group A glycolipid (Ax) with globo-series structure which is specific for blood group A1 erythrocytes: one of the chemical bases for A1 and A2 distinction.

    abstract：:A new blood group A-active glycolipid fraction, termed Ax, showing a chromatographic mobility between Aa and Ab was found in blood group A1 erythrocytes but not in A2 erythrocytes. Ax was identified by its conversion to "globo H" by alpha-N-acetylgalactosaminidase and by 1H-NMR spectroscopy as GalNAc alpha l----3[Fuc ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(84)91585-7

    authors： Clausen H,Watanabe K,Kannagi R,Levery SB,Nudelman E,Arao-Tomono Y,Hakomori S

    更新日期：1984-10-30 00:00:00

  • Clathrin-coated vesicles from human placenta contain GTP-binding proteins.

    abstract：:Biochemical and morphological techniques were used to investigate the GTP-binding proteins of clathrin-coated vesicles. Binding of [3H]GTP to clathrin coats was demonstrated by electron microscopic autoradiography. Purified coated vesicles bound 5.2 pmol [35S]GTP gamma S/mg protein. Addition of GTP or GTP gamma S, but...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)90505-2

    authors： Lenhard JM,Levy MA,Stahl PD

    更新日期：1991-01-15 00:00:00

  • Isolation and characterization of (+)-1,1a-dihydroxy-1-hydrofluoren-9-one formed by angular dioxygenation in the bacterial catabolism of fluorene.

    abstract：:Transformation of fluorene by washed cells of fluorene-grown Pseudomonas sp. F274 yielded 1,la-dihydroxy-1-hydrofluoren-9-one (up to 100 mg/l) as the stable product of angular dioxygenation of 9-fluorenone. Structural identity of the angular keto-diol was established by 13C- and 1H-NMR, gas chromatography- and direct ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.1591

    authors： Selifonov SA,Grifoll M,Gurst JE,Chapman PJ

    更新日期：1993-05-28 00:00:00

  • Evidence for a regulatory action of vanadate on protein phosphorylation in brain microvessels.

    abstract：:We investigated the action of vanadate on protein phosphorylation in microvessels isolated from rat brain. We found that a stimulation of protein phosphorylation from 32P-ATP occurs, in the presence of different concentrations of vanadate, 10(-3) M being the most effective dose. This action was time-dependent, and it ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)92289-4

    authors： Catalán RE,Martínez AM,Aragonés MD,Díaz G

    更新日期：1989-09-15 00:00:00