Membrane association of leucyl-tRNA synthetase during leucine starvation in Escherichia coli.

abstract：:Our goal was to characterize the glucose transporter in synaptosomes and to compare it to the different forms of transporter already identified. Cross-reactivity with antibodies to the human erythrocyte transporter, Km of glucose uptake, reversibility of NEM inhibition of transport, and insulin sensitivity were all ex...

journal_title：Biochemical and biophysical research communications

authors： Bhattacharyya MV,Brodsky JL

更新日期：1988-09-15 00:00:00

abstract：:NEMO is an essential regulatory component of the IkappaB kinase (IKK) complex, which controls activation of the NF-kappaB signaling pathway. Herein, we show that NEMO exists as a disulfide-bonded dimer when isolated from several cell types and analyzed by SDS-polyacrylamide gel electrophoresis under non-reducing condi...

journal_title：Biochemical and biophysical research communications

authors： Herscovitch M,Comb W,Ennis T,Coleman K,Yong S,Armstead B,Kalaitzidis D,Chandani S,Gilmore TD

更新日期：2008-02-29 00:00:00

abstract：:To gain further information on the role of mitochondrial transcription factor A (TFAM) in mitochondrial biogenesis, we studied the post-translational modifications of the protein in 6- and 28-month-old rat liver. Mass spectrometry and immunoblot analysis revealed that TFAM was acetylated at a single lysine residue and...

journal_title：Biochemical and biophysical research communications

authors： Dinardo MM,Musicco C,Fracasso F,Milella F,Gadaleta MN,Gadaleta G,Cantatore P

更新日期：2003-01-31 00:00:00

abstract：:Polyribosomes were isolated without the use of detergents, irradiated with ultraviolet light and labelled in the presence of (gamma-32P) adenosine 5'-triphosphate. Poly(A)+RNA-protein structures separated by chromatography on oligo (dT)-cellulose contained up to 1o crosslinked proteins as shown by SDS-polyacrylamide g...

journal_title：Biochemical and biophysical research communications

authors： Schweiger A,Kostka G,Weiss E

更新日期：1986-04-14 00:00:00

abstract：:We have cloned the cDNA encoding human PICK1 (protein interacting with C kinase 1), a PDZ domain-containing protein of 415 amino acids, and also identified the Drosophila homologue by search of the databank. Northern blot analysis shows a single mRNA of about 2.0 kb ubiquitously expressed in human tissues. Although PI...

journal_title：Biochemical and biophysical research communications

authors： Takeya R,Takeshige K,Sumimoto H

更新日期：2000-01-07 00:00:00

abstract：:Erythroid band 4.1 enhances the binding of erythroid spectrin to phosphatidylserine vesicles under conditions of ionic strength and at protein concentrations similar to those in the red cell. The extent of enhancement depends on the concentration of band 4.1; at 2 microM 4.1, spectrin binding increases approximately 1...

journal_title：Biochemical and biophysical research communications

authors： Takeshita K,MacDonald RI,MacDonald RC

更新日期：1993-02-26 00:00:00

abstract：:In this report, we demonstrate that HL-60 nuclei isolated in calcium but not EGTA containing buffers specifically bind PE and express approximately 37,000 receptor sites/nucleus. Nuclear phorbol ester binding is lost by isolation in the absence of calcium, but can be repleted by the addition of partially purified prot...

journal_title：Biochemical and biophysical research communications

authors： Kraft AS,Appling C,Berkow RL

更新日期：1987-04-14 00:00:00

abstract：:Recent clinical studies have shown that inorganic arsenic trioxide (As(2)O(3)) at low concentrations induces complete remission with minimal toxicity in patients with refractory acute promyelocytic leukemia (APL). Preclinical studies suggest that As(2)O(3) induces apoptosis and possibly differentiation in APL cells. L...

journal_title：Biochemical and biophysical research communications

authors： Ora I,Bondesson L,Jönsson C,Ljungberg J,Pörn-Ares I,Garwicz S,Pâhlman S

更新日期：2000-10-14 00:00:00

abstract：:We have previously provided evidence for a mechanism by which acyl DHAP is converted enzymatically to O-alkyl DHAP. This mechanism involves, in part, the formation of an endiol of acyl DHAP, loss of the fatty acid by splitting of the DHAP carbon-1 to oxygen bond and the gain of a long chain fatty alcohol. It has been ...

journal_title：Biochemical and biophysical research communications

authors： Friedberg SJ,Weintraub ST,Peterson D,Satsangi N

更新日期：1987-06-30 00:00:00

abstract：:We describe a new approach to DNA hybridization assays using metal-enhanced fluorescence. Thiolated oligonucleotides were bound to silver particles on a glass substrate. Addition of a complementary fluorescein-labeled oligonucleotide resulted in a dramatic time-dependent 12-fold increase in fluorescence intensity duri...

journal_title：Biochemical and biophysical research communications

authors： Malicka J,Gryczynski I,Lakowicz JR

更新日期：2003-06-20 00:00:00

abstract：:Multidrug resistance protein 1 (MRP1) is a member of the ATP-binding cassette superfamily. Using the energy provided by ATP hydrolysis, it transports a broad spectrum of substrates across the plasma membrane, including hormones, leukotriene C(4), bile salts, and anti-cancer drugs. Recent works have suggested that P-gl...

journal_title：Biochemical and biophysical research communications

authors： Cerf E,Gasper R,Belani JD,Rychnovsky S,Chang XB,Buyse F,Ruysschaert JM

更新日期：2007-04-20 00:00:00

abstract：:Dexamethasone (Dex) induces direct cytotoxicity to cultured osteoblasts. The benzimidazole derivative compound 991 ("C991") is a novel and highly-efficient AMP-activated protein kinase (AMPK) activator. Here, in both MC3T3-E1 osteoblastic cells and primary murine osteoblasts, treatment with C991 activated AMPK signali...

journal_title：Biochemical and biophysical research communications

authors： Xu YY,Chen FL,Ji F,Fei HD,Xie Y,Wang SG

更新日期：2018-01-01 00:00:00

abstract：:Insulin-like growth factor 1 receptor (IGF1R) is expressed in many types of solid tumors including non-small cell lung cancer (NSCLC), and enhanced activation of IGF1R is thought to reflect cancer progression. Epithelial-mesenchymal transition (EMT) has been established as one of the mechanisms responsible for cancer ...

journal_title：Biochemical and biophysical research communications

authors： Nurwidya F,Takahashi F,Kobayashi I,Murakami A,Kato M,Minakata K,Nara T,Hashimoto M,Yagishita S,Baskoro H,Hidayat M,Shimada N,Takahashi K

更新日期：2014-12-12 00:00:00

abstract：:Missense mutations in the enzyme, glycine N-methyltransferase (GNMT) have been shown to be one cause of persistent isolated hypermethioninaemia in humans. These mutations were first identified by metabolite analysis and were shown to be L49P, N140S, and H176N by gene sequencing. Here we report the kinetic and conforma...

journal_title：Biochemical and biophysical research communications

authors： Luka Z,Wagner C

更新日期：2003-12-26 00:00:00

abstract：:Several lines of evidence provided by other workers indicate that within the same species thyroid hormone binding protein, the beta-subunit of prolyl hydroxylase, and protein disulfide isomerase are the same protein. We sought to determine if glycosylation site binding protein, a lumenal protein of the endoplasmic ret...

journal_title：Biochemical and biophysical research communications

authors： Kimura H,Noiva R,Mizunaga T,Yamauchi K,Horiuchi R,Cheng SY,Lennarz WJ

更新日期：1990-08-16 00:00:00

abstract：:Sorting motifs are involved in the transport of diverse proteins. In the present study, we identified a hydrophobic peptide (WRPWRNFWWSIRVPWRRN) that was able to target enhanced green fluorescent protein- or DsRed2-enriched vesicular-like sub-compartments of the endoplasmic reticulum (ER). Analysis of mutation constru...

journal_title：Biochemical and biophysical research communications

authors： Nie YW,Wang M,Zhang P,Huan L,Liu DJ

更新日期：2016-04-22 00:00:00

abstract：:We have identified and characterized insulin receptors on Chinese hamster ovary (CHO) cells. Insulin binds in a time, temperature and pH dependent fashion and insulin analogues compete for 125I-insulin binding in order of their biological potencies. Furthermore, two CHO cell glycosylation mutants, B4-2-1, lacking high...

journal_title：Biochemical and biophysical research communications

authors： Podskalny J,McElduff A,Gorden P

更新日期：1984-11-30 00:00:00

abstract：:BIK, a member of the Bcl-2 family, has been reported to induce cell apoptosis but the underlying mechanisms are not well delineated. We used siRNA targeting caspase-12 to examine the effect of caspase-12 on apoptosis induced by BIK. In this study, we show that caspase-12 was activated by BIK. With caspase-12 knocked d...

journal_title：Biochemical and biophysical research communications

authors： Zhao X,Sun Y,Yu H,Ye L,Zhang L,Lu J,Yuan Y,Qian G,Ge S

更新日期：2007-08-10 00:00:00

abstract：:Because the expression of EBNA1 is prevalent in all EBV-associated tumors, it has become one of the most attractive drug targets for the discovery of anti-EBV compounds. In a cell-based reporter system, EBNA1 consistently upregulated the transcription of an oriP-Luc mini-EBV episome by 6- to 8-fold. The treatment of c...

journal_title：Biochemical and biophysical research communications

authors： Chen YL,Tsai HL,Peng CW

更新日期：2012-01-20 00:00:00

abstract：:We have recently shown that optimal growth temperature (T(opt)) is one of the factors that influence genomic GC in prokaryotes. Our results have been disputed by Marashi and Ghalanbor, who claim that the correlations we show are not "robust" because the elimination of some points (arbitrarily chosen) leads, in some fa...

journal_title：Biochemical and biophysical research communications

authors： Musto H,Naya H,Zavala A,Romero H,Alvarez-Valin F,Bernardi G

更新日期：2005-05-06 00:00:00

abstract：:High mobility group box 1 (HMGB1) was recently shown to be an important extracellular mediator of severe vascular inflammatory disease, sepsis. Lysozyme (LYZ) has been shown to bind to bacterial lipopolysaccharide (LPS) and have a potential for playing a role in the therapy of inflammatory diseases. However, the effec...

journal_title：Biochemical and biophysical research communications

authors： Lee W,Park EJ,Kwak S,Kim Y,Na DH,Bae JS

更新日期：2015-04-17 00:00:00

abstract：:An enzyme-linked immunosorbent assay of nucleic acid sequence-based amplification (NASBA-ELISA) was developed for molecular detection of Mycobacterium tuberculosis. The primers targeting 16S rRNA were used for the amplification of bacterial RNA by the isothermal digoxigenin (DIG)-labeling NASBA process, resulting in t...

journal_title：Biochemical and biophysical research communications

authors： Gill P,Ramezani R,Amiri MV,Ghaemi A,Hashempour T,Eshraghi N,Ghalami M,Tehrani HA

更新日期：2006-09-08 00:00:00

abstract：:The role of the cytoplasmic domain of human Fc gamma RII in cellular activation was studied by transfecting P815 cells with different isoforms or deletion mutants of this receptor and then assaying protein tyrosine phosphorylation after crosslinking with anti-CD32 monoclonal antibodies. Fc gamma RIIa, but not Fc gamma...

journal_title：Biochemical and biophysical research communications

authors： Liu Z,Pudiak D,Looney RJ

更新日期：1994-06-15 00:00:00

abstract：:Matrix metalloproteinases (MMPs) play an important role in the pathogenesis of cardiovascular diseases and are modified in response to a variety of stimuli such as bioactive peptides, cytokines and/or grown factors. In this study, we demonstrated that angiotensin II (Ang II) induces a time- and dose-dependent increase...

journal_title：Biochemical and biophysical research communications

authors： Jiménez E,Pérez de la Blanca E,Urso L,González I,Salas J,Montiel M

更新日期：2009-03-20 00:00:00

abstract：:Although antisense strategy has been at the center of interest in gene therapy, little is known about application of this strategy to cardiac diseases because of the lack of a suitable delivery method into the heart. Therefore, we compared the transfection efficiency of antisense oligodeoxynucleotides (ODN) using HVJ-...

journal_title：Biochemical and biophysical research communications

authors： Aoki M,Morishita R,Higaki J,Moriguchi A,Kida I,Hayashi S,Matsushita H,Kaneda Y,Ogihara T

更新日期：1997-02-24 00:00:00

abstract：:Microsomal cytochrome P450 reductase catalyzes the one-electron transfer from NADPH via FAD and FMN to various electron acceptors, such as cytochrome P450s or to some anti-cancer quinone drugs. This results in generation of free radicals and toxic oxygen metabolites, which can contribute to the cytotoxicity of these c...

journal_title：Biochemical and biophysical research communications

authors： Kwaśnicka-Crawford DA,Vincent SR

更新日期：2005-10-21 00:00:00

abstract：:One pathogenesis of the obesity-associated complications is that consistent with increased body fat mass, the elevation of adipose tissue-derived cytokines inflicts a low-grade chronic inflammation, which ultimately leads to metabolic disorders. Adipocytes and macrophages in visceral adipose (VA) have been confirmed t...

journal_title：Biochemical and biophysical research communications

authors： Yue GP,Du LR,Xia T,He XH,Qiu H,Xu LH,Chen XD,Feng SQ,Yang ZQ

更新日期：2005-08-05 00:00:00

abstract：:We investigated immunohistochemically the localization of p33, an endogenous substrate protein for an arginine-specific ADP-ribosyltransferase in chicken liver. Polymorphonuclear-pseudo-eosinophilic granulocytes (heterophils) in interlobular connective tissues of the liver were exclusively and strongly stained with th...

journal_title：Biochemical and biophysical research communications

authors： Osago H,Mishima K,Tsuchiya M,Tanigawa Y,Umeno T,Shimoyama M

更新日期：1991-10-15 00:00:00

abstract：:The human homolog of the Drosophila discs large tumor suppressor protein (hDLG) functions as a scaffolding protein that facilitates the transmission of diverse downstream signals. Here we show that hDLG interacts through its PDZ domains with the carboxy-terminal S/TXV motif of the mitochondrial ribosomal protein S-34 ...

journal_title：Biochemical and biophysical research communications

authors： Ogawa F,Adachi S,Kohu K,Shige K,Akiyama T

更新日期：2003-01-17 00:00:00

abstract：:The primary amino acid sequence of the carboxyl-terminal portion of the alpha 3 chain of chicken type VI collagen (K-VI) presents a 58-residue motif with a high degree of homology with members of the Kunitz serine-proteinase inhibitors family. This module was cloned, expressed in E. coli, purified and compared to the ...

journal_title：Biochemical and biophysical research communications

authors： Bearz A,Tolazzi G,Leonardi A,Pucillo C,Tell G,Colombatti A,Formisano S

更新日期：1995-10-24 00:00:00