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Band 4.1 enhances spectrin binding to phosphatidylserine vesicles.

Abstract：

:Erythroid band 4.1 enhances the binding of erythroid spectrin to phosphatidylserine vesicles under conditions of ionic strength and at protein concentrations similar to those in the red cell. The extent of enhancement depends on the concentration of band 4.1; at 2 microM 4.1, spectrin binding increases approximately 10-fold (to 600 mumoles/mole lipid). The Kd is 0.5 microM, as measured by SDS-PAGE of protein-bound vesicles recovered by ficoll gradient centrifugation. The 4.1-enhanced binding of spectrin was also measured by a gel filtration assay. The electrostatic nature of the enhancement of spectrin binding is indicated by its dependence on the phosphatidylserine content of the vesicles.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Takeshita K,MacDonald RI,MacDonald RC

doi

10.1006/bbrc.1993.1198

subject

Has Abstract

pub_date

1993-02-26 00:00:00

pages

165-71

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(83)71198-8

journal_volume

191

pub_type

杂志文章

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