Formation of N-nitrosodiphenylamine from 1,1-diphenylhydrazine by rat liver microsomal preparations.

abstract：:The activation of vitamin D requires 25-hydroxylation in the liver and 1alpha-hydroxylation in the kidney. However, it remains unclear which enzyme is relevant to vitamin D 25-hydroxylation. Recently, human CYP2R1 has been reported to be a potential candidate for a hepatic vitamin D 25-hydroxylase. Thus, vitamin D met...

journal_title：Biochemical and biophysical research communications

authors： Shinkyo R,Sakaki T,Kamakura M,Ohta M,Inouye K

更新日期：2004-11-05 00:00:00

abstract：:The chaperonin from the acidothermophilic archaeon, Sulfolobus sp. Strain 7, is composed of two kinds of subunits designated as Scp alpha and Scp beta. In this study, we characterized the recombinant Scp alpha and Scp beta, which were separately expressed in Escherichia coli. Both of them were able to assemble to homo...

journal_title：Biochemical and biophysical research communications

authors： Yoshida T,Yohda M,Suzuki M,Yazaki K,Miura K,Endo I

更新日期：1998-01-26 00:00:00

abstract：:Polygonatum cyrtonema lectin (PCL), a mannose/sialic acid-binding plant lectin, has recently drawn a rising attention for cancer biologists because PCL bears remarkable anti-tumor activities and thus inducing programmed cell death (PCD) including apoptosis and autophagy in cancer cells. In this review, we focus on exp...

journal_title：Biochemical and biophysical research communications

authors： Wang SY,Yu QJ,Bao JK,Liu B

更新日期：2011-03-25 00:00:00

abstract：:In rat adrenal dispersed cells, both vanadyl and vanadate inhibited ACTH-stimulated steroidogenesis and the formation of cAMP, whereas these compounds did not inhibit the cAMP-dependent steroidogenesis. Then, the membrane fraction was prepared and activated by various secretagogues including ACTH, Gpp(NH)p, GTP gamma ...

journal_title：Biochemical and biophysical research communications

authors： Hayashi Y,Kimura T

更新日期：1985-08-15 00:00:00

abstract：:Aberrantly expressed microRNAs (miRNAs) are frequently associated with the aggressive malignant behavior of human cancers, including clear cell renal cell carcinoma (ccRCC). Based on the preliminary deep sequencing data, we hypothesized that miR-187 may play an important role in ccRCC development. In this study, we fo...

journal_title：Biochemical and biophysical research communications

authors： Zhao J,Lei T,Xu C,Li H,Ma W,Yang Y,Fan S,Liu Y

更新日期：2013-08-23 00:00:00

abstract：:Lysophosphatidylcholine (LPC) has been attributed a pro-inflammatory role in atherosclerosis. Cell culture studies have identified stimulation of cytokine expression and chemotaxis by micromolar (muM) concentrations of LPC. In the present study we have investigated if LPC, in similarity with many other lipid mediators...

journal_title：Biochemical and biophysical research communications

authors： Olofsson KE,Andersson L,Nilsson J,Björkbacka H

更新日期：2008-05-30 00:00:00

abstract：:We studied the subcellular distribution and the properties of [3H] mepyramine binding in the porcine vascular smooth muscle. A close correlation was observed between the specific binding activity of [3H] mepyramine and the extent of the enrichment of sarcolemmal marker enzyme in 4 subfractions obtained by sucrose dens...

journal_title：Biochemical and biophysical research communications

authors： Nishimura J,Kanaide H,Miwa N,Nakamura M

更新日期：1985-01-16 00:00:00

abstract：:Molecular structure of the ligand binding domain of hAhR has been modelled by homology modelling techniques and used for docking simulations with dioxin and nine more xenobiotics and endogenous ligands. The study evidences that different sites may bind these ligands, whereas only one binding site has been previously i...

journal_title：Biochemical and biophysical research communications

authors： Salzano M,Marabotti A,Milanesi L,Facchiano A

更新日期：2011-09-23 00:00:00

abstract：:Interactions between C1q and other subunits of C1 were analyzed by sucrose gradient ultracentrifugation. A zone of dilute, radioiodine labelled C1q was sedimented through uniform concentrations of either C1r2C1s2, C1r2, C1r2 or C1s(2). The dissociation constants were found to be 3 x 10(-9) M and 6 x 10(-9) M for C1r2C...

journal_title：Biochemical and biophysical research communications

authors： Lakatos S

更新日期：1987-12-16 00:00:00

abstract：:Until today, 14 isoforms of mammalian calpains have been identified, including calpain-5. The C. elegans calpain-5 homologue tra-3 is reported to be essential for necrotic neuronal cell death. In this study, we cloned and characterized rat calpain-5, which is highly homologous to human and mouse sequences. The nucleot...

journal_title：Biochemical and biophysical research communications

authors： Waghray A,Wang DS,McKinsey D,Hayes RL,Wang KK

更新日期：2004-11-05 00:00:00

abstract：:A cDNA clone encoding human aldosterone synthase cytochrome P-450 (P-450aldo) has been isolated from a cDNA library derived from human adrenal tumor of a patient suffering from primary aldosteronism. The insert of the clone contains an open reading frame encoding a protein of 503 amino acid residues together with a 3 ...

journal_title：Biochemical and biophysical research communications

authors： Kawainoto T,Mitsuuchi Y,Ohnishi T,Ichikawa Y,Yokoyama Y,Sumimoto H,Toda K,Miyahara K,Kuribayashi I,Nakao K

更新日期：1990-11-30 00:00:00

abstract：:The anomeric proton of the reducing N-acetylglucosamine residue of beta-N,N'-diacetylchitobiose exhibited an unusual lineshape indicative of virtual coupling in the 250 MHz proton NMR spectrum. The two-dimensional J-resolved spectrum contained four lines associated with this proton, and this pattern can be used to dis...

journal_title：Biochemical and biophysical research communications

authors： Bruch RC,Bruch MD,Noggle JH,White HB 3rd

更新日期：1984-09-17 00:00:00

abstract：:We found a novel protein-protein interaction between ubiquitin-specific protease 15 (USP15) and skeletal muscle LIM protein 1 (SLIM1): USP15 and SLIM1 directly bound under cell-free conditions and co-immunoprecipitated from the lysates of the cells, where they were co-expressed; and USP15 deubiquitinated SLIM1, result...

journal_title：Biochemical and biophysical research communications

authors： Isumi Y,Hirata T,Saitoh H,Miyakawa T,Murakami K,Kudoh G,Doi H,Ishibashi K,Nakajima H

更新日期：2011-02-11 00:00:00

abstract：:It is controversial whether osteopontin (OP) is expressed in glomeruli and involved in glomerular diseases. We examined whether the OP expression is present at gene and protein levels in cultured rat mesangial cells (MCs). Northern blotting revealed a 1.7 kb OP-mRNA expression in MCs. Fetal calf serum (FCS) and TNF-al...

journal_title：Biochemical and biophysical research communications

authors： Nagasaki T,Ishimura E,Shioi A,Jono S,Inaba M,Nishizawa Y,Morii H,Otani S

更新日期：1997-04-07 00:00:00

abstract：:Multidrug resistance protein 1 (MRP1/ABCC1) is a 190kDa member of the ATP-binding cassette (ABC) superfamily of transmembrane transporters that is clinically relevant for its ability to confer multidrug resistance by actively effluxing anticancer drugs. Knowledge of the atomic structure of MRP1 is needed to elucidate ...

journal_title：Biochemical and biophysical research communications

authors： DeGorter MK,Conseil G,Deeley RG,Campbell RL,Cole SP

更新日期：2008-01-04 00:00:00

abstract：:Renal homeostasis is regulated by the interplay among different cell types in the kidneys including endothelial cells. In the present study we investigated the phenotypic regulation of endothelial cells by BRG1, a chromatin remodeling protein, in a mouse model of obstructive nephropathy (ON). We report that endothelia...

journal_title：Biochemical and biophysical research communications

authors： Liu L,Mao L,Xu Y,Wu X

更新日期：2019-09-17 00:00:00

abstract：:A mathematical model that includes biochemical interactions among the PTS system, phosphofructokinase (PFK), and pyruvate kinase (PK) is used to evaluate the dynamic behavior of the glycolytic pathway of Escherichia coli under steady-state conditions. The influence of ADP, phosphoenolpyruvate (PEP), and fructose-6-pho...

journal_title：Biochemical and biophysical research communications

authors： Diaz Ricci JC

更新日期：2000-04-29 00:00:00

abstract：:It has been demonstrated that ubiquitin-conjugated proteins were accumulated by ectopically-expressed S5a as well as the ubiquitin-interacting motifs of S5a (S5a-UIMs). In this study, we further found that free S5a-UIMs stabilized only a subset of proteasomal substrates including p53, c-Fos, c-Jun, and p27 but not bet...

journal_title：Biochemical and biophysical research communications

authors： Elangovan M,Shin DY,Yoo YJ

更新日期：2009-10-30 00:00:00

abstract：:We have identified and characterized insulin receptors on Chinese hamster ovary (CHO) cells. Insulin binds in a time, temperature and pH dependent fashion and insulin analogues compete for 125I-insulin binding in order of their biological potencies. Furthermore, two CHO cell glycosylation mutants, B4-2-1, lacking high...

journal_title：Biochemical and biophysical research communications

authors： Podskalny J,McElduff A,Gorden P

更新日期：1984-11-30 00:00:00

abstract：:The bacteriophage T7 binary expression system is widely used in vitro for high level selective expression of cloned genes but its application to in vivo models has not yet been investigated. In the present work, we show that coinjection into fertilized zebrafish eggs of pE1T7R, an expression plasmid bearing the T7 RNA...

journal_title：Biochemical and biophysical research communications

authors： Verri T,Argenton F,Tomanin R,Scarpa M,Storelli C,Costa R,Colombo L,Bortolussi M

更新日期：1997-08-28 00:00:00

abstract：:Cilia/flagella are evolutionarily conserved cellular organelles. In this study, we demonstrated that Dunaliella salina Peroxiredoxin 1 (DsPrdx1) localized to the flagella and basal bodies, and was involved in flagellar disassembly. The link between DsPrdx1 and flagella of Dunaliella salina (D. salina) encouraged us to...

journal_title：Biochemical and biophysical research communications

authors： Gong F,Liu H,Li J,Xue L,Zhang M

更新日期：2014-02-14 00:00:00

abstract：:ATG4B belongs to the autophagin family of cysteine proteases required for autophagy, an emerging target of cancer therapy. Developing pharmacological ATG4B inhibitors is a very active area of research. However, detailed studies on the role of ATG4B during anticancer therapy are lacking. By analyzing PC-3 and C4-2 pros...

journal_title：Biochemical and biophysical research communications

authors： Tran E,Chow A,Goda T,Wong A,Blakely K,Rocha M,Taeb S,Hoang VC,Liu SK,Emmenegger U

更新日期：2013-11-29 00:00:00

abstract：:Th2 type immune responses are essential for protective immunity against parasites and play crucial roles in allergic disorders. Helminth parasites secrete a variety of proteases for their infectious cycles including for host entry, tissue migration, and suppression of host immune effector cell function. Furthermore, a...

journal_title：Biochemical and biophysical research communications

authors： Nishikado H,Fujimura T,Taka H,Mineki R,Ogawa H,Okumura K,Takai T

更新日期：2015-05-01 00:00:00

abstract：:Recombinant scorpion anti-excitation peptide (rANEP) has previously been expressed using the pET32a system and purified via affinity chromatography. However, rANEP is expressed in BL21(DE3) cells as an inclusion body, and the affinity tag can not be removed. To overcome this problem, we used a variety of protein, DsbA...

journal_title：Biochemical and biophysical research communications

authors： Han T,Ming H,Deng L,Zhu H,Liu Z,Zhang J,Song Y

更新日期：2017-01-01 00:00:00

abstract：:Recently, we developed a novel system known as Full-length cDNA Over-eXpressor (FOX) gene hunting [T. Ichikawa, M. Nakazawa, M. Kawashima, H. Iizumi, H. Kuroda, Y. Kondou, Y. Tsuhara, K. Suzuki, A. Ishikawa, M. Seki, M. Fujita, R. Motohashi, N. Nagata, T. Takagi, K. Shinozaki, M. Matsui, The FOX hunting system: an alt...

journal_title：Biochemical and biophysical research communications

authors： Fujita M,Mizukado S,Fujita Y,Ichikawa T,Nakazawa M,Seki M,Matsui M,Yamaguchi-Shinozaki K,Shinozaki K

更新日期：2007-12-14 00:00:00

abstract：:The purpose of the present study is to characterize the ENaC-mediated Na+ absorption in human upper airway epithelia, nasal cavity, and paranasal sinus. To perform the purpose, we obtained epithelial cells from human nasal polyp (NP) and paranasal sinus mucosa (PSM) by endoscopic surgery. We measured the short-circuit...

journal_title：Biochemical and biophysical research communications

authors： Yasuda M,Niisato N,Miyazaki H,Iwasaki Y,Hama T,Dejima K,Hisa Y,Marunaka Y

更新日期：2007-10-26 00:00:00

abstract：:The effects of different lysophospholipids on endothelium-dependent hyperpolarization by acetylcholine were examined in rat mesenteric arteries. Lysophosphatidylcholine with 14 or longer carbon acyl chain significantly inhibited the hyperpolarization, while that with 12 or lesser carbon acyl chain was without effect. ...

journal_title：Biochemical and biophysical research communications

authors： Fukao M,Hattori Y,Kanno M,Sakuma I,Kitabatake A

更新日期：1996-10-14 00:00:00

abstract：:Cell-free translation is one potential approach to the production of functional transmembrane proteins. We have now examined various detergents as supplements to a wheat-germ cell-free system in order to optimize the production and subsequent purification of a functional model transmembrane protein, bacteriorhodopsin....

journal_title：Biochemical and biophysical research communications

authors： Genji T,Nozawa A,Tozawa Y

更新日期：2010-10-01 00:00:00

abstract：:The forkhead transcription factor FoxA1 is thought to be involved in mammary tumorigenesis. However, the precise role of FoxA1 in breast cancer development is controversial. We examined expression of FoxA1 in 35 human breast cancer cell lines and compared it with that of ErbB2, a marker of poor prognosis in breast can...

journal_title：Biochemical and biophysical research communications

authors： Yamaguchi N,Ito E,Azuma S,Honma R,Yanagisawa Y,Nishikawa A,Kawamura M,Imai J,Tatsuta K,Inoue J,Semba K,Watanabe S

更新日期：2008-01-25 00:00:00

abstract：:Ischemic stroke is a major cause of death and disability worldwide. Hyperneuroinflammation significantly contributes to ischemic stroke. Bromodomain-containing protein 4 (BRD4) is a member of the Bromo and Extra-Terminal (BET) family, and promotes inflammatory response in various types of tissue and cells. Thereby, we...

journal_title：Biochemical and biophysical research communications

authors： Zhou Y,Gu Y,Liu J

更新日期：2019-11-12 00:00:00