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Molecular cloning and characterization of rat and human calpain-5.

Abstract：

:Until today, 14 isoforms of mammalian calpains have been identified, including calpain-5. The C. elegans calpain-5 homologue tra-3 is reported to be essential for necrotic neuronal cell death. In this study, we cloned and characterized rat calpain-5, which is highly homologous to human and mouse sequences. The nucleotide sequence is 87% and 93% identical with human and mouse calpain-5, respectively. The protein sequence is well conserved, showing 96% identity in mouse and 92% in human. RT-PCR analysis revealed strong expression of calpain-5 in rat lungs, kidneys, and brain while week expression in heart, whereas in rat brain regions it is ubiquitously expressed. The mRNA expression in different human tissues showed equal expression. However, in human brain regions calpain-5 was strongly expressed in hypothalamus, thalamus, cerebellum, and frontal lobe. Western blot analysis on human neuroblastoma SH-SY5Y cells demonstrated calcium-dependent processing of calpain-5, despite the absence of calmodulin-like domain.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Waghray A,Wang DS,McKinsey D,Hayes RL,Wang KK

doi

10.1016/j.bbrc.2004.09.012

subject

Has Abstract

pub_date

2004-11-05 00:00:00

pages

46-51

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(04)02046-7

journal_volume

324

pub_type

杂志文章

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