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Identification of a long form of cystatin from human saliva by rapid microbore HPLC mapping.

Abstract：

:Microbore HPLC methodology permits rapid and sensitive mapping of human saliva proteins. Saliva is sampled and processed in less than one hour, greatly reducing the likelihood of artifactual protein degradation. As little as 50 microliters of saliva yields proteins in sufficient quantities and purity to obtain amino terminal sequences directly. By this route we have discovered a 14 kDa protein extremely homologous to Cystatin S, but amino-terminally extended by eight amino acids.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Hawke DH,Yuan PM,Wilson KJ,Hunkapiller MW

doi

10.1016/0006-291x(87)91571-3

subject

Has Abstract

pub_date

1987-06-30 00:00:00

pages

1248-53

issue

3

eissn

0006-291X

issn

1090-2104

pii

0006-291X(87)91571-3

journal_volume

145

pub_type

杂志文章

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