Osteopontin gene expression and protein synthesis in cultured rat mesangial cells.

abstract：:(15)N and (1)HN chemical shift data and (15)N relaxation studies have been used to characterise the binding of N-phenyl-naphthylamine (NPN) to mouse major urinary protein (MUP). NPN binds in the beta-barrel cavity of MUP, hydrogen bonding to Tyr120 and making extensive non-bonded contacts with hydrophobic side chains....

journal_title：Biochemical and biophysical research communications

authors： Pertinhez TA,Ferrari E,Casali E,Patel JA,Spisni A,Smith LJ

更新日期：2009-12-25 00:00:00

abstract：:ATP is the universal energy currency for all cells but has cellular concentrations of 2-12 mM, much higher than required for its classic functions. RNA-recognition motif (RRM) constitutes one of the most abundant domains in eukaryotes and most heterogeneous nuclear ribonucleoproteins (hnRNP) contain RRM domains which ...

journal_title：Biochemical and biophysical research communications

authors： Dang M,Kang J,Lim L,Li Y,Wang L,Song J

更新日期：2020-01-29 00:00:00

abstract：:Hypothesis of non-enzymatic recognition of primordial tRNA and mRNA precursors is experimentally approached. DNA hairpins containing a different number of deoxyguanosine residues in the loop are analyzed for their binding ability to a chemically fixed single-strand of oligo(dC). In presence of small Mg2+ concentration...

journal_title：Biochemical and biophysical research communications

authors： Baumann U,Frank R,Blöcker H

更新日期：1988-12-30 00:00:00

abstract：:The Monte Carlo method has been used to simulate the equilibrium properties of a planar array of 94 saturated lipid chains and one monomer of Gramicidin A. Chains are free to move laterally in the layer plane and to change conformation via gauche rotations and long axis rotations in a continuum. All non-hydrogen atoms...

journal_title：Biochemical and biophysical research communications

authors： Xing J,Scott HL

更新日期：1989-11-30 00:00:00

abstract：:Necrotic cells are known to develop characteristic membrane blebs. We measured protein concentration within necrotic blebs and found that it can be reduced by as much as twenty-fold compared to the main cell body (CB). These results raise two questions: 1. Why do proteins vacate the bleb? 2. How can osmotic equilibriu...

journal_title：Biochemical and biophysical research communications

authors： Rana PS,Mudrak NJ,Lopez R,Lynn M,Kershner L,Model MA

更新日期：2017-10-21 00:00:00

abstract：:Here, we show that H-ras(V12) causes the p53-knockout mouse astrocytes (p53-/- astrocytes) to be transformed into brain cancer stem-like cells. H-ras(V12) triggers the p53-/- astrocytes to express a Nestin and a Cd133, which are expressed in normal and cancer neural stem cells. H-ras(V12) also induces the formation of...

journal_title：Biochemical and biophysical research communications

authors： Lee JS,Gil JE,Kim JH,Kim TK,Jin X,Oh SY,Sohn YW,Jeon HM,Park HJ,Park JW,Shin YJ,Chung YG,Lee JB,You S,Kim H

更新日期：2008-01-18 00:00:00

abstract：:The tissue targeting and rate of clearance of cobra venom factor (CVF) from the circulation was studied in mice by intravenous or intraperitoneal injection of radioiodinated CVF. In both modes of administrations, CVF was targeted mainly to liver. CVF injected directly into the blood was cleared from the circulation wi...

journal_title：Biochemical and biophysical research communications

authors： Fu Q,Satyaswaroop PG,Gowda DC

更新日期：1997-02-13 00:00:00

abstract：:The envelope glycoprotein E2 is the major immunodominant protein of the classical swine fever virus and can induce neutralizing antibodies and protective host-immune responses in infected swine. We designed, expressed, and purified multi-epitope protein (GST-BT22) that contains a tandem repeat of the E2 antigenic-dete...

journal_title：Biochemical and biophysical research communications

authors： Tian H,Hou X,Liu X

更新日期：2013-02-08 00:00:00

abstract：:Protein kinase C (PKC) and the GTPase-activating protein GAP can be detected in immunoprecipitates of mouse epidermis and lung cytosol obtained with either anti-GAP or anti-PKC antisera. The PKC in the immune-complex phosphorylates the coprecipitated GAP protein. Moreover, purified recombinant GAP is phosphorylated in...

journal_title：Biochemical and biophysical research communications

authors： Gschwendt M,Kittstein W,Marks F

更新日期：1993-07-15 00:00:00

abstract：:Cell cycle arrest is essential for initiation of muscle differentiation in myoblasts. Given the previously described essential role for p38 MAPK in myogenesis, we undertook the present study to investigate the role of p38 MAPK in the cell cycle arrest that initiates muscle differentiation. p38 MAPK activity increased ...

journal_title：Biochemical and biophysical research communications

authors： Lee J,Hong F,Kwon S,Kim SS,Kim DO,Kang HS,Lee SJ,Ha J,Kim SS

更新日期：2002-11-15 00:00:00

abstract：:The discovery of clock genes revealed the major molecular components responsible for circadian time-keeping in mammals, but the mechanism by which autonomic nervous system may control circadian rhythm and its relationship to metabolism is unclear. As the Cry1 and Cry2 genes are indispensable for molecular core oscilla...

journal_title：Biochemical and biophysical research communications

authors： Ikeda H,Yong Q,Kurose T,Todo T,Mizunoya W,Fushiki T,Seino Y,Yamada Y

更新日期：2007-12-21 00:00:00

abstract：:The 55- (H-UK) and 36-kDa forms (L-UK) of human urinary urokinase lost most of esterase activity toward acetyl-glycyl-L-lysine methyl ester upon reductive cleavage of 3 SS bonds with dithiothreitol in the presence of the competitive inhibitor, N alpha-benzoyl-L-arginine amide (BAA), bound to polyacrylyl azide with C16...

journal_title：Biochemical and biophysical research communications

authors： Miwa N,Obata Y,Suzuki A

更新日期：1983-04-29 00:00:00

abstract：:Interaction of camel lens zeta-crystallin with aspirin was investigated by activity and fluorescence measurements. Aspirin minimally inhibited the oxidoreductase activity of the enzyme and weakly quenched its fluorescence. However, significant fluorescence quenching of zeta-crystallin coincided with the appearance of ...

journal_title：Biochemical and biophysical research communications

authors： Bazzi MD

更新日期：2002-04-26 00:00:00

abstract：:Deficient apoptosis of activated T cells can result in immunological disorders. Molecules associated with energy and metabolisms are suggested to be involved in pathogenesis of immune diseases, but remain uninvestigated. In the present study we reported that glibenclamide exerted a new pharmacological effect on inflam...

journal_title：Biochemical and biophysical research communications

authors： Ouyang Z,Wang X,Meng Q,Feng L,Sun Y,Wu X,Xu Q

更新日期：2017-05-27 00:00:00

abstract：:Wild type p53 protein requires posttranslational modification within a carboxy-terminal negative regulatory domain to activate DNA binding and transcription. Binding of monoclonal antibody PAb421 to the carboxy-terminal domain reproduces this activation. In the absence of PAb421, we found that wild type p53 bound acti...

journal_title：Biochemical and biophysical research communications

authors： Kaku S,Albor A,Kulesz-Martin M

更新日期：2001-01-12 00:00:00

abstract：:Resistin, an adipocyte secreted cysteine rich hormone has been implicated as molecular link between obesity and type 2 diabetes in a murine model. Although, at the protein level mouse and human resistin show remarkable similarities with respect to conserved cysteine residues, the physiological role of human resistin i...

journal_title：Biochemical and biophysical research communications

authors： Raghu P,Ghosh S,Soundarya K,Haseeb A,Aruna B,Ehtesham NZ

更新日期：2004-01-16 00:00:00

abstract：:A P1 phage clone containing the near complete protein coding region of the human histo-blood group ABO gene locus was isolated and characterized. The insert was estimated at approximately 41 kp, and an approximately 20 kbp BamHI fragment contained the near complete coding region as evaluated by Southern blot analysis....

journal_title：Biochemical and biophysical research communications

authors： Bennett EP,Steffensen R,Clausen H,Weghuis DO,van Kessel AG

更新日期：1995-01-05 00:00:00

abstract：:Nucleolar protein p40/EBP2 is a proliferation-associated antigen that interacts with Epstein-Barr virus nuclear antigen 1 (EBNA1) to maintain the Epstein-Barr virus (EBV) episomes. The yeast p40/EBP2 functions in the processing of 27S-A into 27S-B ribosomal RNA. The present study reports high evolutionary conservation...

journal_title：Biochemical and biophysical research communications

authors： Henning D,Valdez BC

更新日期：2001-05-04 00:00:00

abstract：:Hsp90 interacts with proteins that mediate signaling pathways involved in the regulation of essential processes such as proliferation, cell cycle control, angiogenesis and apoptosis. Hsp90 inhibition is therefore an attractive strategy for blocking abnormal pathways that are crucial for cancer cell growth. In the pres...

journal_title：Biochemical and biophysical research communications

authors： Zuo K,Li D,Pulli B,Yu F,Cai H,Yuan X,Zhang X,Lv Z

更新日期：2012-05-04 00:00:00

abstract：:Glycosaminoglycans were prepared from the Engelbreth-Holm-Swarm mouse tumor. Enzymatic analysis demonstrated heparan sulfate (95.8%) and chondroitinase ABC-sensitive galactosaminoglycans (4.2%). HPLC analysis of the disaccharide units showed that heparan sulfate chains were undersulfated on average, comprising approxi...

journal_title：Biochemical and biophysical research communications

authors： Sugahara K,Okumura Y,Yamashina I

更新日期：1989-07-14 00:00:00

abstract：OBJECTIVE:The accumulation of lipids in macrophages contributes to the development of atherosclerosis. Strategies to reduce lipid accumulation in macrophages may have therapeutic potential for preventing and treating atherosclerosis and cardiovascular complications. The antidiabetic drug metformin has been reported to ...

journal_title：Biochemical and biophysical research communications

authors： Song J,Ren P,Zhang L,Wang XL,Chen L,Shen YH

更新日期：2010-02-26 00:00:00

abstract：:Insulin-like growth factor binding protein (IGFBP) gene expression and IGFBP secretion were investigated in a retinoic acid (RA)-resistant subline (RROI) of MCF-7 human breast carcinoma cells. Our results demonstrate that RRO-I cells constitutively secrete higher levels of IGFBP-3 and IGFBP-5. In addition, we found th...

journal_title：Biochemical and biophysical research communications

authors： Sheikh MS,Shao ZM,Chen JC,Clemmons DR,Roberts CT Jr,LeRoith D,Fontana JA

更新日期：1992-11-16 00:00:00

abstract：:Antibodies subjected to SDS-polyacrylamide gel electrophoresis and transferred to nitrocellulose paper, have been found to retain the ability to bind specific antigen. This has been demonstrated for two groups of antibodies, directed to a) a large molecular weight glycoprotein of the human milk fat globule and b) huma...

journal_title：Biochemical and biophysical research communications

authors： Mather S,Taylor-Papadimitriou J

更新日期：1985-12-31 00:00:00

abstract：:The Vpr protein of human immunodeficiency virus type 1 (HIV-1) is incorporated into the virion by the Gag polyprotein precursor Pr55gag. The importance of the p6gag sequence at the C-terminal end of Pr55gag has a crucial role in Vpr incorporation. To identify the Gag sequences directly involved in Vpr binding, we comp...

journal_title：Biochemical and biophysical research communications

authors： Li MS,Garcia-Asua G,Bhattacharyya U,Mascagni P,Austen BM,Roberts MM

更新日期：1996-01-05 00:00:00

abstract：:Functional biopolymer scaffolds are in high demand for tissue regeneration. In this study, we incorporated functionalized CNT in collagen or silk protein solution to generate biocomposite fibers by electrospinning. The addition of CNT reinforced the strength of the scaffolds and rendered the fibers electrical conducti...

journal_title：Biochemical and biophysical research communications

authors： Chi N,Wang R

更新日期：2018-09-26 00:00:00

abstract：:Acute kidney injury induced by renal ischemia-reperfusion (IR) is a prominent risk factor in the development towards renal fibrosis. Ras-related C3 botulinum toxin substrate 1(Rac1) has been involved in the pathophysiology of fibrotic disorders. But the role of Rac1 in the pathogenesis of IR-induced renal fibrosis is ...

journal_title：Biochemical and biophysical research communications

authors： Liang H,Huang J,Huang Q,Xie YC,Liu HZ,Wang HB

更新日期：2018-09-18 00:00:00

abstract：:Prion diseases are characterized by the accumulation of a pathological form of prion protein (PrP(Sc)), which behaves as an infectious agent. Here we developed an in vitro co-culture system to analyze the PrP(Sc) transmission from ScN2a cell, which persistently retains PrP(Sc), to naïve N2a cell. In this cell-to-cell ...

journal_title：Biochemical and biophysical research communications

authors： Tanaka M,Hara H,Nishina H,Hanada K,Hagiwara K,Maehama T

更新日期：2010-07-02 00:00:00

abstract：:We tested usefulness of a target enrichment system SureSelect, a comprehensive viral nucleic acid detection method, for rapid identification of viral pathogens in feces samples of cattle, pigs and goats. This system enriches nucleic acids of target viruses in clinical/field samples by using a library of biotinylated R...

journal_title：Biochemical and biophysical research communications

authors： Oba M,Tsuchiaka S,Omatsu T,Katayama Y,Otomaru K,Hirata T,Aoki H,Murata Y,Makino S,Nagai M,Mizutani T

更新日期：2018-01-08 00:00:00

abstract：:Recently, T cell cytokines such as IL-17 and IFN-γ have been shown to play important roles in the progression of brain injury induced by ischemia. We have shown that IL-23 from infiltrated macrophages activates γδT cells, thereby inducing IL-17 from these cells. However, deletion of the IL-23 gene in mice showed a mor...

journal_title：Biochemical and biophysical research communications

authors： Konoeda F,Shichita T,Yoshida H,Sugiyama Y,Muto G,Hasegawa E,Morita R,Suzuki N,Yoshimura A

更新日期：2010-11-19 00:00:00

abstract：:Oxygen resistant N2-fixing Escherichia coli, which can grow at a high oxygen concentration in a nitrogen-free medium, were produced by several times of cultivation under a condition of 0.03% oxygen. Six isolated resistant strains could grow at an oxygen concentration ten times that of the parent strain. The nif-genes ...

journal_title：Biochemical and biophysical research communications

authors： Iwahashi H,Someya J

更新日期：1990-04-16 00:00:00