Chemically induced viral resistance in single preimplantation bovine embryos.

abstract：:We previously showed that SIRT1 deacetylase inhibits proliferation of hepatocellular carcinoma cells expressing hepatitis B virus (HBV) X protein (HBX), by destabilization of β-catenin. Here, we report another role for SIRT1 in HBX-mediated resistance to oxidative stress. Ectopic expression and enhanced activity of SI...

journal_title：Biochemical and biophysical research communications

authors： Srisuttee R,Koh SS,Malilas W,Moon J,Cho IR,Jhun BH,Horio Y,Chung YH

更新日期：2012-12-07 00:00:00

abstract：:Accumulation of misfolded proteins within the lumen of the mammalian endoplasmic reticulum (ER) activates the unfolded protein response (UPR). ATF6 and Ire1p are ER-associated proteins that control UPR-specific transcription systems in mammals; UPR signaling involves cleavage of ATF6 and splicing of XBP1 mRNA initiate...

journal_title：Biochemical and biophysical research communications

authors： Shang J,Lehrman MA

更新日期：2004-04-30 00:00:00

abstract：:In this study, we investigated hybrid and non-hybrid actomyosin models including key contractile proteins: actin, myosin, and tropomyosin. These proteins were isolated from the rabbit skeletal muscle and the catch muscle of the mussel Crenomytilus grayanus. Our results confirmed literature data on an unusual ability o...

journal_title：Biochemical and biophysical research communications

authors： Shelud'ko NS,Vyatchin IG,Lazarev SS,Shevchenko UV

更新日期：2015-08-21 00:00:00

abstract：:We have identified and characterized insulin receptors on Chinese hamster ovary (CHO) cells. Insulin binds in a time, temperature and pH dependent fashion and insulin analogues compete for 125I-insulin binding in order of their biological potencies. Furthermore, two CHO cell glycosylation mutants, B4-2-1, lacking high...

journal_title：Biochemical and biophysical research communications

authors： Podskalny J,McElduff A,Gorden P

更新日期：1984-11-30 00:00:00

abstract：:We recently reported that oviduct epithelial cells in culture produce endothelin (ET) and postulated that ET could play an important role in the contraction of the oviduct. In the present study using an organ chamber myograph system, we evaluated the contractile effects of ET-1 on bovine oviduct ampullary-isthmus segm...

journal_title：Biochemical and biophysical research communications

authors： Rosselli M,Imthurn B,Macas E,Keller PJ,Dubey RK

更新日期：1994-05-30 00:00:00

abstract：:Macrophage colony stimulating factor (CSF-1) is a member of a family of glycoproteins that are necessary for the normal proliferation and differentiation of myeloid progenitor cells. The human CSF-1 gene has previously been assigned to chromosome 5 using somatic cell hybrids, and further localized to 5q33 by in situ h...

journal_title：Biochemical and biophysical research communications

authors： Saltman DL,Dolganov GM,Hinton LM,Lovett M

更新日期：1992-02-14 00:00:00

abstract：:The muscarinic acetylcholine receptor was solubilized, in a sensitive form for GTP and Na+, from bovine cerebral cortex using a zwitterionic detergent 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane sulfonate. The solubilized muscarinic receptor displayed characteristics as follows: (1) high affinity to nanomolar co...

journal_title：Biochemical and biophysical research communications

authors： Kuno T,Shirakawa O,Tanaka C

更新日期：1983-05-16 00:00:00

abstract：:Gonadotropin releasing hormone (GnRH), preincubated with cultured rat pituitary cells, induced down regulation of GnRH receptors in a time- and dose-dependent manner. The specific binding was inhibited by 50% after 30 min and maximal inhibition (70%) was obtained after 75 min preincubation with 1 microM GnRH. Preincub...

journal_title：Biochemical and biophysical research communications

authors： Hazum E,Schvartz I

更新日期：1984-12-14 00:00:00

abstract：:Leda-1/Pianp is a type I transmembrane protein expressed by CNS cells, murine melanoma cell line B16F10 and rat liver sinusoidal endothelial cells. The early steps of posttranslational modifications of Leda-1/Pianp have been described to include glycosylation and processing by proprotein convertases. Here, we comprehe...

journal_title：Biochemical and biophysical research communications

authors： Biswas S,Adrian M,Weber J,Evdokimov K,Winkler M,Géraud C

更新日期：2016-09-02 00:00:00

abstract：:Vomeronasal organs of female Wistar rats were exposed with sprayed urine preparations of male Wistar rats prior to sacrifice. Exposure to crude urine and ultrafiltrated urine preparation (<5000 Da) induced significant Fos expression, which is correlated with cellular activity, in the mitral/tufted cell layer of the ac...

journal_title：Biochemical and biophysical research communications

authors： Tsujikawa K,Kashiwayanagi M

更新日期：1999-06-24 00:00:00

abstract：:Topoisomerase 1 (TOP1) poisons like camptothecin (CPT) are currently used in cancer chemotherapy but these compounds can have damaging, off-target effects on neurons leading to cognitive, sensory and motor deficits. To understand the molecular basis for the enhanced sensitivity of neurons to CPT, we examined the effec...

journal_title：Biochemical and biophysical research communications

authors： Rossi SL,Lumpkin CJ,Harris AW,Holbrook J,Gentillon C,McCahan SM,Wang W,Butchbach MER

更新日期：2016-10-14 00:00:00

abstract：:We have studied the internalization and subsequent nuclear localization of [125I]-rTNF-alpha in a human osteoblast-like cell line, HOS TE85. TNF-alpha is rapidly internalized and optimum cellular levels are achieved in approximately 20 minutes at 37 degrees C. A portion of the internalized cytokine is translocated to ...

journal_title：Biochemical and biophysical research communications

authors： Panagakos FS,Kumar S

更新日期：1994-06-30 00:00:00

abstract：:While the appetite-stimulating hormone ghrelin can act to acutely modulate electrical activity of neurons in the appetite regulating network, it also has a role in regulating neuronal outgrowth, synaptic connectivity and intrinsic electrophysiological properties. In this study, we investigated whether ghrelin may caus...

journal_title：Biochemical and biophysical research communications

authors： Huang S,Lee SA,Oswald KE,Fry M

更新日期：2015-10-30 00:00:00

abstract：:Here we enhanced the stability and biophysical properties of mRFP1 through a combination of canonical and non-canonical amino acid mutagenesis. The global replacement of proline residue with (2S, 4R)-4-fluoroproline [(4R)-FP] into mRFP1 led to soluble protein but lost its fluorescence, whereas (2S, 4S)-4-fluoroproline...

journal_title：Biochemical and biophysical research communications

authors： Deepankumar K,Nadarajan SP,Ayyadurai N,Yun H

更新日期：2013-11-01 00:00:00

abstract：:Inflammation has multifaceted role in cancer progression including initiation, promotion and invasion by affecting the immune surveillance and associated signaling pathways. Inflammation facilitates the over-expression of cytokines, chemokines and growth factors involved in progression of different cancers including b...

journal_title：Biochemical and biophysical research communications

authors： Suman S,Sharma PK,Rai G,Mishra S,Arora D,Gupta P,Shukla Y

更新日期：2016-04-08 00:00:00

abstract：:UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I (GlcNAc-T I; EC 2.4.1.101) is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. We have isolated two overlapping genomic DNA clones which span 18 kilobases (kb) containing a single 2.5 kb exon for Gl...

journal_title：Biochemical and biophysical research communications

authors： Hull E,Sarkar M,Spruijt MP,Höppener JW,Dunn R,Schachter H

更新日期：1991-04-30 00:00:00

abstract：:Nuclear orphan receptor TLX is an essential regulator of the growth of neural stem cells. However, its exact function in pancreatic islet cells is still unknown. In the present study, gene expression profiling analysis revealed that overexpression of TLX in beta cell line MIN6 causes suppression of 176 genes and upreg...

journal_title：Biochemical and biophysical research communications

authors： Shi X,Xiong X,Dai Z,Deng H,Sun L,Hu X,Zhou F,Xu Y

更新日期：2015-12-04 00:00:00

abstract：:Cytosolic free calcium concentration ([Ca(2+)]i) is a central signalling element for the maintenance of endothelial barrier function. Under physiological conditions, it is controlled within narrow limits. Metabolic inhibition during ischemia/reperfusion, however, induces [Ca(2+)]i overload, which results in barrier fa...

journal_title：Biochemical and biophysical research communications

authors： Bosche B,Schäfer M,Graf R,Härtel FV,Schäfer U,Noll T

更新日期：2013-05-03 00:00:00

abstract：:Apolipoprotein B (apoB) mRNA editing involves a site-specific cytidine to uridine transition catalyzed by the cytidine deaminase, APOBEC-1, in the context of and regulated by a multi-protein-containing editosome. ApoB mRNA editing in vivo is subject to tissue specific, developmental and metabolic regulation. We demons...

journal_title：Biochemical and biophysical research communications

authors： Van Mater D,Sowden MP,Cianci J,Sparks JD,Sparks CE,Ballatori N,Smith HC

更新日期：1998-11-18 00:00:00

abstract：:A guinea pig adrenal hydroxysteroid sulfotransferase (gpHST2) has been cloned that is distinct from guinea pig hydroxysteroid sulfotransferase that stereoselectively acts on 3 alpha-hydroxylated neutral steroids (gp3 alpha HST, redesignated gpHST1). The deduced amino acid sequences for gpHST1 and gpHST2 are 86% identi...

journal_title：Biochemical and biophysical research communications

authors： Luu NX,Driscoll WJ,Martin BM,Strott CA

更新日期：1995-12-26 00:00:00

abstract：:The D protein (16 kDa) is part of a protein core, common to U1, U2, U5, U4/U6 small nuclear RNA containing ribonucleoprotein particles. Monoclonal antibodies reactive with the D protein were used in quantitative dot blotting and Western blotting to demonstrate that this protein was a component of salt resistant nuclea...

journal_title：Biochemical and biophysical research communications

authors： Harris SG,Smith HC

更新日期：1988-05-16 00:00:00

abstract：:Information on the immune response against H5N1 within the lung is lacking. Here we describe the sustained antiviral immune responses, as indicated by the expression of MxA protein and IFN-alpha mRNA, in autopsy lung tissue from an H5N1-infected patient. H5N1 infection of primary bronchial/tracheal epithelial cells an...

journal_title：Biochemical and biophysical research communications

authors： Thitithanyanont A,Engering A,Uiprasertkul M,Ekchariyawat P,Wiboon-Ut S,Kraivong R,Limsalakpetch A,Kum-Arb U,Yongvanitchit K,Sa-Ard-Iam N,Rukyen P,Mahanonda R,Kawkitinarong K,Auewarakul P,Utaisincharoen P,Sirisinha S,Mason

更新日期：2010-08-06 00:00:00

abstract：:NEMO is an essential regulatory component of the IkappaB kinase (IKK) complex, which controls activation of the NF-kappaB signaling pathway. Herein, we show that NEMO exists as a disulfide-bonded dimer when isolated from several cell types and analyzed by SDS-polyacrylamide gel electrophoresis under non-reducing condi...

journal_title：Biochemical and biophysical research communications

authors： Herscovitch M,Comb W,Ennis T,Coleman K,Yong S,Armstead B,Kalaitzidis D,Chandani S,Gilmore TD

更新日期：2008-02-29 00:00:00

abstract：:ATP binding cassette (ABC) transporters have a functional unit formed by two transmembrane domains and two nucleotide binding domains (NBDs). ATP-bound NBDs dimerize in a head-to-tail arrangement, with two nucleotides sandwiched at the dimer interface. Both NBDs contribute residues to each of the two nucleotide-bindin...

journal_title：Biochemical and biophysical research communications

authors： Zoghbi ME,Altenberg GA

更新日期：2014-01-03 00:00:00

abstract：:The localization of gene expression of a low M(r) GTP-binding protein, ram p25, was examined throughout the entire brain of adult rat by in situ hybridization. ram-mRNA was expressed predominantly in anterior and intermediate lobes of pituitary gland (hypophysis), and weakly in hippocampal formation. In other parts of...

journal_title：Biochemical and biophysical research communications

authors： Nagata K,Sakagami H,Kondo H,Nozawa Y

更新日期：1995-12-26 00:00:00

abstract：:Endothelin tightly bound to rabbit aortic strips and caused a prolonged vasoconstriction both in the presence and absence of extracellular Ca2+, although only partial constriction (20-30%) developed in the latter case, indicating that its action may not be limited to the opening of a calcium channel. The endothelin-in...

journal_title：Biochemical and biophysical research communications

authors： Sugiura M,Inagami T,Hare GM,Johns JA

更新日期：1989-01-16 00:00:00

abstract：:It is well-established that non-random patterns in coding DNA sequence (CDS) features can be partially explained by translational selection. Recent extensions of microarray and proteomic expression data have stimulated many genome-wide investigations of the relationships between gene expression and various CDS feature...

journal_title：Biochemical and biophysical research communications

authors： Wu G,Nie L,Freeland SJ

更新日期：2007-07-13 00:00:00

abstract：:Novel conformation-specific antibodies were raised against a cyclic chimeric dodecapeptidyl multiple antigen peptide (cCD-MAP) constructed with a spacer-armed Gly-Asp dipeptide and two pentapeptides (S(169)-Q(170)-K(171)-E(172)-G(173) of CCR5 and E(179)-A(180)-D(181)-D(182)-R(183) of CXCR4) which are components of the...

journal_title：Biochemical and biophysical research communications

authors： Misumi S,Takamune N,Ido Y,Hayashi S,Endo M,Mukai R,Tachibana K,Umeda M,Shoji S

更新日期：2001-08-03 00:00:00

abstract：:Methamphetamine induces several cardiac dysfunctions, which leads to arrhythmia, cardiac failure and sudden cardiac death. Although these cardiac alterations elicited by methamphetamine were thought to be due to an indirect action of methamphetamine, namely, an excessive catecholamine release from synaptic terminals, ...

journal_title：Biochemical and biophysical research communications

authors： Sugimoto K,Okamura K,Tanaka H,Takashima S,Ochi H,Yamamoto T,Matoba R

更新日期：2009-12-25 00:00:00

abstract：:A cDNA for the rat bone morphogenetic protein (BMP) type IA receptor (BMPR-IA) was isolated from a dental pulp cell cDNA library. The rat BMPR-IA cDNA encodes a protein of 532 amino acids with a single transmembrane domain and a putative serine/threonine kinase domain. The overall amino acid sequence identity between ...

journal_title：Biochemical and biophysical research communications

authors： Takeda K,Oida S,Ichijo H,Iimura T,Maruoka Y,Amagasa T,Sasaki S

更新日期：1994-10-14 00:00:00