当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > High-resolution crystal structure of HA33 of botulinum neurotoxin type B progenitor toxin complex.

High-resolution crystal structure of HA33 of botulinum neurotoxin type B progenitor toxin complex.

Abstract：

:Botulinum neurotoxins (BoNTs) are produced as progenitor toxin complexes (PTCs) by Clostridium botulinum. The PTCs are composed of BoNT and non-toxic neurotoxin-associated proteins (NAPs), which serve to protect and deliver BoNT through the gastrointestinal tract in food borne botulism. HA33 is a key NAP component that specifically recognizes host carbohydrates and helps enrich PTC on the intestinal lumen preceding its transport across the epithelial barriers. Here, we report the crystal structure of HA33 of type B PTC (HA33/B) in complex with lactose at 1.46Å resolution. The structural comparisons among HA33 of serotypes A-D reveal two different HA33-glycan interaction modes. The glycan-binding pockets on HA33/A and B are more suitable to recognize galactose-containing glycans in comparison to the equivalent sites on HA33/C and D. On the contrary, HA33/C and D could potentially recognize Neu5Ac as an independent receptor, whereas HA33/A and B do not. These findings indicate that the different oral toxicity and host susceptibility observed among different BoNT serotypes could be partly determined by the serotype-specific interaction between HA33 and host carbohydrate receptors. Furthermore, we have identified a key structural water molecule that mediates the HA33/B-lactose interactions. It provides the structural basis for development of new receptor-mimicking compounds, which have enhanced binding affinity with HA33 through their water-displacing moiety.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Lee K,Lam KH,Kruel AM,Perry K,Rummel A,Jin R

doi

10.1016/j.bbrc.2014.03.008

subject

Has Abstract

pub_date

2014-04-04 00:00:00

pages

568-73

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(14)00430-6

journal_volume

446

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Identification of covalently attached fatty acids in the hydrophobic membrane-binding domain of human erythrocyte acetylcholinesterase.

    abstract：:Human erythrocyte acetylcholinesterase is an amphipathic enzyme whose hydrophobic membrane-binding domain can be selectively labeled with a lipophilic photoreagent and removed by digestion with papain. In this paper we demonstrate that methanolysis releases covalently bound fatty acids from the hydrophobic domain and ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(85)90950-7

    authors： Roberts WL,Rosenberry TL

    更新日期：1985-12-17 00:00:00

  • Regulation of lipoprotein lipase secretion by mouse peritoneal macrophages.

    abstract：:Resident mouse peritoneal macrophages in culture spontaneously secrete lipoprotein lipase. Secretion of lipoprotein lipase was 10-fold greater in thioglycollate-elicited and 6-fold greater in mineral oil-elicited macrophages. The increase in enzyme secretion was dependent on protein synthesis and glycosylation of the ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(87)90443-8

    authors： Goldberg DI,Khoo JC

    更新日期：1987-01-15 00:00:00

  • Adaptor proteins GIR1 and GIR2. I. Interaction with the repressor GLABRA2 and regulation of root hair development.

    abstract：:Plants use specialized root outgrowths, termed root hairs, to enhance acquisition of nutrients and water, help secure anchorage, and facilitate interactions with soil microbiome. One of the major regulators of this process is GLABRA2 (GL2), a transcriptional repressor of root hair differentiation. However, regulation ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.05.084

    authors： Wu R,Citovsky V

    更新日期：2017-07-01 00:00:00

  • Role of N-terminal domain of streptokinase in protein transport.

    abstract：:Streptokinase (SK), an extracellular protein of several haemolytic strains of Streptococcus, is utilized as a potent thrombolytic agent for the treatment of various myocardial disorders. Functional properties of SK remain unchanged when the first 13 N-terminal amino acid (aa) residues are removed. At present, role of ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.1504

    authors： Pratap J,Kaur J,RajaMohan G,Singh D,Dikshit KL

    更新日期：1996-10-03 00:00:00

  • Localized decrease of beta-catenin contributes to the differentiation of human embryonic stem cells.

    abstract：:Human embryonic stem cells (hESC) are pluripotent, and can be directed to differentiate into different cell types for therapeutic applications. To expand hESCs, it is desirable to maintain hESC growth without differentiation. As hESC colonies grow, differentiated cells are often found at the periphery of the colonies,...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.05.116

    authors： Lam H,Patel S,Wong J,Chu J,Lau A,Li S

    更新日期：2008-08-08 00:00:00

  • Covalent conjugation of Groucho with SUMO-1 modulates its corepressor activity.

    abstract：:Groucho is a corepressor that forms a macromolecular complex for its corepressor activity, in which HDAC1 is an essential component for the modulation of chromatin structure and transcriptional repression of target genes. Here, we show that Groucho is covalently conjugated with small ubiquitin-related modifier-1 (SUMO...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.12.043

    authors： Ahn JW,Lee YA,Ahn JH,Choi CY

    更新日期：2009-01-30 00:00:00

  • Polymorphisms of TNFalpha, IL1beta, and IL1RN genes in chronic obstructive pulmonary disease.

    abstract：:It is recognized that genetic factors play a role in the susceptibility to COPD. COPD is characterized by airflow limitation. Chronic inflammation causes small airway disease and parenchymal destruction, leading to the airflow limitation. Polymorphisms in pro-inflammatory cytokine genes may confer a risk for the devel...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.02.099

    authors： Hegab AE,Sakamoto T,Saitoh W,Nomura A,Ishii Y,Morishima Y,Iizuka T,Kiwamoto T,Matsuno Y,Massoud HH,Massoud HM,Hassanein KM,Sekizawa K

    更新日期：2005-04-22 00:00:00

  • A point mutation in a plasma membrane Ca(2+)-ATPase gene causes deafness in Wriggle Mouse Sagami.

    abstract：:The spontaneous mutant, Wriggle Mouse Sagami (wri), is thought to be a model of hereditary hearing losses in humans. Here we report that the plasma membrane Ca(2+)-ATPase type 2 (PMCA2) gene is mutated in the wri mouse. A G-to-A transition was detected in wri, changing Glu-to-Lys within a conserved transmembrane domai...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1102

    authors： Takahashi K,Kitamura K

    更新日期：1999-08-11 00:00:00

  • Transverse motion of spin-labeled 3,3',5-triiodo-L-thyronine in phospholipid bilayers.

    abstract：:Using electron spin resonance stop-flow technique, the transverse motion (flip-flop) of 3-([alpha-carboxy-4-(4-hydroxy-3-iodophenoxy)-3,5- diiodophenethyl]carbamoyl)-2,2,5,5-tetramethyl-3-pyrrolin (T3-SL) in dipalmitoyl L-alpha-phosphosphatidylcholine (DPPC) membranes was evaluated. At 22 degrees C, the electron spin ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(85)91817-0

    authors： Lai CS,Korytowski W,Niu CH,Cheng SY

    更新日期：1985-08-30 00:00:00

  • Effect of phosphorylation on 68 KDa neurofilament subunit protein assembly by the cyclic AMP dependent protein kinase in vitro.

    abstract：:The effect of phosphorylation by cyclic AMP dependent protein kinase on the assembly of the core-forming 68 KDa neurofilament subunit protein (NF-L) was studied in vitro by fluorescence energy transfer and electron microscopy. Phosphorylation of unassembled NF-L in a low ionic strength buffer by cyclic AMP dependent p...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)90394-3

    authors： Nakamura Y,Takeda M,Angelides KJ,Tanaka T,Tada K,Nishimura T

    更新日期：1990-06-15 00:00:00

  • Protein kinase PKN1 associates with TRAF2 and is involved in TRAF2-NF-kappaB signaling pathway.

    abstract：:PKN1 is a fatty acid and Rho-activated serine/threonine protein kinase whose catalytic domain is highly homologous to protein kinase C (PKC) family. In yeast two-hybrid screening for PKN1 binding proteins, we identified tumor necrosis factor alpha (TNFalpha) receptor-associated factor 2 (TRAF2). TRAF2 is one of the ma...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2003.12.148

    authors： Gotoh Y,Oishi K,Shibata H,Yamagiwa A,Isagawa T,Nishimura T,Goyama E,Takahashi M,Mukai H,Ono Y

    更新日期：2004-02-13 00:00:00

  • Cytotoxicity resulting from addition of HIV-1 Nef N-terminal peptides to yeast and bacterial cells.

    abstract：:The Nef protein of human immunodeficiency type 1 (HIV-1) has been implicated in diverse intracellular functions; however, extracellular functions have been less studied. Nef and the N-terminus of Nef possess membrane-perturbing and fusogenic activities in artificial membranes that also cause cytotoxicity to human cell...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.6349

    authors： Macreadie IG,Lowe MG,Curtain CC,Hewish D,Azad AA

    更新日期：1997-03-27 00:00:00

  • The mouse iduronate sulfatase gene: identification of a novel transcript.

    abstract：:Complementary DNAs, cDNAs, coding for iduronate sulfatase (IDS, E.C.3.1.6.13) have been isolated and characterized in man and mouse. The murine cDNA (MTA16) was highly homologous to the human counterpart. By screening the same mouse thymus cDNA library another cDNA (MTA13) was isolated: it is 1831 bp long and is ident...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.1925

    authors： Daniele A,Russo T,Ballabio A,Di Natale P

    更新日期：1993-08-16 00:00:00

  • Real-time visualization of PH domain-dependent translocation of phospholipase C-delta1 in renal epithelial cells (MDCK): response to hypo-osmotic stress.

    abstract：:Green fluorescent protein (GFP)-tagged phospholipase C (PLC)-delta1 and its mutants were expressed in Madin-Darby canine kidney (MDCK) cells. GFP-PLC-delta1 or the GFP-tagged pleckstrin homology (PH) domain of PLC-delta1 itself was found to be predominantly localized at the plasma membrane. The DeltaPH mutant or a sit...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.9936

    authors： Fujii M,Ohtsubo M,Ogawa T,Kamata H,Hirata H,Yagisawa H

    更新日期：1999-01-19 00:00:00

  • Protective effect of recombinant soluble neprilysin against β-amyloid induced neurotoxicity.

    abstract：:A few decades ago, researchers found emerging evidence showing that a number of sequential events lead to the pathological cascade of Alzheimer's disease (AD) which is caused by the accumulation of amyloid beta (Aβ), a physiological peptide, in the brain. Therefore, regulation of Aβ represents a crucial treatment appr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.06.103

    authors： Oh JH,Choi S,Shin J,Park JS

    更新日期：2016-09-02 00:00:00

  • Genome editing in pluripotent stem cells: research and therapeutic applications.

    abstract：:Recent progress in human pluripotent stem cell (hPSC) and genome editing technologies has opened up new avenues for the investigation of human biology in health and disease as well as the development of therapeutic applications. Gene editing approaches with programmable nucleases have been successfully established in ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章,评审

    doi：10.1016/j.bbrc.2016.02.113

    authors： Deleidi M,Yu C

    更新日期：2016-05-06 00:00:00

  • Inflammatory effect of endosulfan via NF-kappaB activation in macrophages.

    abstract：:Macrophages are essential for the inflammatory response process because they release a wide variety of proinflammatory mediators. Endosulfan is extremely toxic to invertebrates and has been implicated in various mammalian toxicities. However, its influence on production of cytokine or on the functions of macrophages i...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.01.062

    authors： Han EH,Hwang YP,Kim HG,Jeong HG

    更新日期：2007-04-20 00:00:00

  • Aspartic cathepsin D degrades the cytosolic cysteine cathepsin inhibitor stefin B in the cells.

    abstract：:Stefin B is the major general cytosolic protein inhibitor of cysteine cathepsins. Its main function is to protect the organism against the activity of endogenous potentially hazardous proteases accidentally released from lysosomes. In this study, we investigated the possible effect of endosomal/lysosomal aspartic cath...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.07.155

    authors： Železnik TZ,Kadin A,Turk V,Dolenc I

    更新日期：2015-09-18 00:00:00

  • Sonic hedgehog expression during early tooth development in Suncus murinus.

    abstract：:Tooth development is a highly organized process characterized by reciprocal interactions between epithelium and mesenchyme. However, the expression patterns and functions of molecules involved in mouse tooth development are unclear from the viewpoint of explaining human dental malformations and anomalies. Here, we sho...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.08.158

    authors： Miyado M,Ogi H,Yamada G,Kitoh J,Jogahara T,Oda S,Sato I,Miyado K,Sunohara M

    更新日期：2007-11-16 00:00:00

  • Protein kinase C forms a complex with and phosphorylates the GTPase activating protein GAP: phosphorylation by PKC is dependent on tyrosine phosphorylation of GAP and/or a GAP-associated protein.

    abstract：:Protein kinase C (PKC) and the GTPase-activating protein GAP can be detected in immunoprecipitates of mouse epidermis and lung cytosol obtained with either anti-GAP or anti-PKC antisera. The PKC in the immune-complex phosphorylates the coprecipitated GAP protein. Moreover, purified recombinant GAP is phosphorylated in...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.1858

    authors： Gschwendt M,Kittstein W,Marks F

    更新日期：1993-07-15 00:00:00

  • Synergistic potentiation of thiazolidinedione-induced ST 13 preadipocyte differentiation by RAR synergists.

    abstract：:Peroxisome proliferator-activated receptor gamma (PPAR gamma) belongs to a nuclear receptor super family that functions as a master regulator of adipocyte differentiation. PPAR gamma binds its DNA response element together with a partner, retinoid X receptor (RXR), in fat cells. Five RXR ligands (HX600, HX630, DA022, ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.4172

    authors： Sato M,Yajima Y,Kawashima S,Tanaka K,Kagechika H

    更新日期：2001-01-26 00:00:00

  • AGPAT2 deficiency impairs adipogenic differentiation in primary cultured preadipocytes in a non-autophagy or apoptosis dependent mechanism.

    abstract：AIMS:Mutations in 1-acylglycerol-3-phosphate O-acyltransferase 2 (AGPAT2) result in lipodystrophy, insulin resistance and diabetes. Autophagy is required for normal adipogenesis and adipose tissue development. The aim of this study was to determine whether impaired autophagy or excessive cell death underlie the adipoge...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.09.128

    authors： Fernández-Galilea M,Tapia P,Cautivo K,Morselli E,Cortés VA

    更新日期：2015-11-06 00:00:00

  • Modulation of cyclic AMP accumulation in GH3 cells by a phorbol ester and thyroliberin.

    abstract：:4 beta phorbol-12, 13-dibutyrate (PDBu) stimulated cyclic AMP accumulation in GH3 pituitary tumour cells in the presence of isobutylmethylxanthine. This effect persisted after preincubation of cells with cholera or pertussis toxins. In contrast, vasoactive intestinal polypeptide (VIP)-stimulated cyclic AMP accumulatio...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(85)91976-x

    authors： Quilliam LA,Dobson PR,Brown BL

    更新日期：1985-06-28 00:00:00

  • Cell adhesion to ephrinb2 is induced by EphB4 independently of its kinase activity.

    abstract：:Cell to cell interaction in bone marrow is crucial for differentiation of hematopoietic cells. We have shown that EphB4 receptor is expressed in erythroid progenitor and its activation accelerates erythroid differentiation. To elucidate the role of EphB4 activation in erythropoiesis, we analyzed effects of EphB4 on ce...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.07.016

    authors： Sakamoto H,Zhang XQ,Suenobu S,Ohbo K,Ogawa M,Suda T

    更新日期：2004-08-27 00:00:00

  • Tissue distribution, subcellular localization, and enzymatic activity analysis of human SIRT5 isoforms.

    abstract：:SIRT5 is one of the seven mammalian sirtuins which are NAD+-dependent deacylases. In human beings, SIRT5 gene encodes for four SIRT5 protein isoforms, namely SIRT5iso1, SIRT5iso2, SIRT5iso3, and SIRT5iso4. Previous studies have focused mostly on SIRT5iso1. Characteristics regarding localization, activity and tissue di...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.06.073

    authors： Du Y,Hu H,Hua C,Du K,Wei T

    更新日期：2018-09-05 00:00:00

  • Crystal structure of the E. coli tRNA(Arg) aminoacyl stem isoacceptor RR-1660 at 2.0 A resolution.

    abstract：:Due to the redundancy of the genetic code there exist six mRNA codons for arginine and several tRNA(Arg) isoacceptors which translate these triplets to protein within the context of the mRNA. The tRNA identity elements assure the correct aminoacylation of the tRNA with the cognate amino acid by the aminoacyl-tRNA-synt...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.04.158

    authors： Eichert A,Perbandt M,Oberthür D,Schreiber A,Fürste JP,Betzel C,Erdmann VA,Förster C

    更新日期：2009-07-17 00:00:00

  • Phenotypes of ATP-activated current associated with their genotypes of P2X1-6 subunits in neurons innervating tooth-pulp.

    abstract：:To explore the association of the phenotype of ATP-activated current with the genotype of P2X1-6 subunits in nociceptors, we developed a method that allows us to label nociceptive neurons innervating tooth-pulp in rat trigeminal ganglion (TG) neurons using a retrograde fluorescence-tracing method, to record ATP-activa...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.01.156

    authors： Liu Y,Tian X,Wu Y,Chen L,Yi CL,Li ZW,Zhang Y,Li CY

    更新日期：2015-03-13 00:00:00

  • Proteins that bind to the RERMS region of beta amyloid precursor protein.

    abstract：:The main objective of this study was to investigate the biological function of beta amyloid precursor protein (APP), in particular its nerve growth factor-like activity. We hypothesize that the extracellular domain containing the sequence RERMS, amino acids 328-332 of APP(695), represents the active site for this func...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.02.047

    authors： Pawlik M,Otero DA,Park M,Fischer WH,Levy E,Saitoh T

    更新日期：2007-04-20 00:00:00

  • Extended analysis of AL-amyloid protein from abdominal wall subcutaneous fat biopsy: kappa IV immunoglobulin light chain.

    abstract：:In AL-amyloidosis the cause of amyloid fibril formation in beta-pleated sheets from the precursor protein immunoglobulin light chain is not established, but studies of AL-proteins indicate that amino acid substitutions are important in the pathogenesis. Amyloid material was extracted from a subcutaneous fat tissue bio...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.8515

    authors： Olsen KE,Sletten K,Westermark P

    更新日期：1998-04-28 00:00:00

  • MAP kinase-signaling controls nuclear translocation of tripeptidyl-peptidase II in response to DNA damage and oxidative stress.

    abstract：:Reactive oxygen species (ROS) are a continuous hazard in eukaroytic cells by their ability to cause damage to biomolecules, in particular to DNA. Previous data indicated that the cytosolic serine peptidase tripeptidyl-peptidase II (TPPII) translocates into the nucleus of most tumor cell lines in response to gamma-irra...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.06.133

    authors： Preta G,de Klark R,Chakraborti S,Glas R

    更新日期：2010-08-27 00:00:00