当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > The expression of soluble and cell-bound alpha 2,6 sialyltransferase in human colonic carcinoma CaCo-2 cells correlates with the degree of enterocytic differentiation.

The expression of soluble and cell-bound alpha 2,6 sialyltransferase in human colonic carcinoma CaCo-2 cells correlates with the degree of enterocytic differentiation.

Abstract：

:alpha 2,6 sialyltransferase towards the N-acetyllactosaminyl sequence (alpha 2,6 ST, E.C. 2.4.99.1) is one of the major sialyltransferases in human colonic cells; it strongly increases in human colorectal tumors and is largely expressed in fetal and neonatal rat colon. In this study we demonstrate that human colon carcinoma CaCo-2 cells, which differentiate spontaneously into enterocytes when maintained confluent for several days, exhibit a very high expression of alpha 2,6 ST both in the cell-bound and soluble form. When the CaCo-2 cells were cultured on porous membranes the soluble alpha 2,6 ST was mainly detected in the medium collected from the chamber corresponding to the basolateral face of the monolayer. The soluble alpha 2,6 ST could be concentrated and purified from the alpha 2,3 sialyltransferase by affinity chromatography on Blue Sepharose.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Dall'Olio F,Malagolini N,Serafini-Cessi F

doi

10.1016/s0006-291x(05)80039-7

subject

Has Abstract

pub_date

1992-05-15 00:00:00

pages

1405-10

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(05)80039-7

journal_volume

184

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Sez6l2 regulates phosphorylation of ADD and neuritogenesis.

    abstract：:Increasing evidence shows that immune-mediated mechanisms may contribute to the pathogenesis of central nervous system disorders including cerebellar ataxias, as indicated by the aberrant production of neuronal surface antibodies. We previously reported a patient with cerebellar ataxia associated with production of a ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.10.047

    authors： Yaguchi H,Yabe I,Takahashi H,Watanabe M,Nomura T,Kano T,Matsumoto M,Nakayama KI,Watanabe M,Hatakeyama S

    更新日期：2017-12-09 00:00:00

  • Nanomedicine for the molecular diagnosis of cardiovascular pathologies.

    abstract：:Predicting acute clinical events caused by atherosclerotic plaque rupture remains a clinical challenge. Anatomic mapping of the vascular tree provided by standard imaging technologies is not always sufficient for a robust diagnosis. Yet biological mechanisms leading to unstable plaques have been identified and corresp...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章,评审

    doi：10.1016/j.bbrc.2015.06.138

    authors： Juenet M,Varna M,Aid-Launais R,Chauvierre C,Letourneur D

    更新日期：2015-12-18 00:00:00

  • Chemoenzymic preparation of a glycoconjugate polymer having a sialyloligosaccharide: Neu5Ac alpha (2-->3)Gal beta (1-->4)GlcNAc.

    abstract：:Water-soluble polyacrylamide having 3'-sialyl N-acetyl-lactosamine [Neu5Ac alpha (2-->3)Gal beta (1-->4)GlcNAc] was enzymatically prepared by stepwise sugar-elongation on a water-soluble GlcNAc-bearing polyacrylamide. It was demonstrated that the flexible GlcNAc branches of the polymer chains allow quantitative galact...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.1221

    authors： Nishimura S,Lee KB,Matsuoka K,Lee YC

    更新日期：1994-02-28 00:00:00

  • Secretion of N-terminal domain of α-dystroglycan in cerebrospinal fluid.

    abstract：:α-Dystroglycan (α-DG) plays crucial roles in maintaining the stability of cells. We demonstrated previously that the N-terminal domain of α-DG (α-DG-N) is secreted by cultured cells into the culture medium. In the present study, to clarify its function in vivo, we generated a monoclonal antibody against α-DG-N and inv...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.06.150

    authors： Saito F,Saito-Arai Y,Nakamura-Okuma A,Ikeda M,Hagiwara H,Masaki T,Shimizu T,Matsumura K

    更新日期：2011-07-29 00:00:00

  • Biochemical and genetic characterization of the structure of yeast ornithine decarboxylase.

    abstract：:The ornithine decarboxylase gene of S. cerevisiae encodes a predicted protein of approximately 53 kD highly homologous with the ornithine decarboxylase of other species. However, the native enzyme has been reported as an 86 kD protein. Our molecular sieve analysis indicated a Mr = 110,000 for the native enzyme. SDS-PA...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)90831-0

    authors： Fonzi WA

    更新日期：1989-08-15 00:00:00

  • mTOR activation is critical for betulin treatment in renal cell carcinoma cells.

    abstract：:Betulin, a natural product isolated from the bark of the birch trees, exhibits multiple anticancer effects. Activation of mTOR signaling pathway has been found in numerous cancers, including renal cell carcinoma (RCC). Here, we attempted to study whether mTOR signaling was essential for betulin to treat RCC. Based on ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.11.153

    authors： Cheng W,Ji S,Zhang H,Han Z,Liu Q,Wang J,Ping H

    更新日期：2017-01-22 00:00:00

  • Identification of a specific transport system for L-arginine in human platelets.

    abstract：:The present study demonstrates that human platelets possess a specific L-arginine transport system able to provide adequate amounts of L-arginine for endogenous nitric oxide production. L-arginine uptake takes place through a saturable high affinity carrier-mediated Na(+)-independent process which is significantly inh...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.1125

    authors： Vasta V,Meacci E,Farnararo M,Bruni P

    更新日期：1995-01-26 00:00:00

  • Genomic analyses and expression evaluation of thaumatin-like gene family in the cacao fungal pathogen Moniliophthora perniciosa.

    abstract：:Thaumatin-like proteins (TLPs) are found in diverse eukaryotes. Plant TLPs, known as Pathogenicity Related Protein (PR-5), are considered fungal inhibitors. However, genes encoding TLPs are frequently found in fungal genomes. In this work, we have identified that Moniliophthora perniciosa, a basidiomycete pathogen tha...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.09.054

    authors： Franco Sde F,Baroni RM,Carazzolle MF,Teixeira PJ,Reis O,Pereira GA,Mondego JM

    更新日期：2015-10-30 00:00:00

  • The sugar composition and partial structure of the self-induced endogenous elicitor from potato.

    abstract：:The self-induced endogenous elicitor isolated from potato having molecular weight 9200 was degraded with trifluoroacetic acid to determine the sugar composition. The requisite composition of the elicitor was estimated to be a 2:3:4:7 mixture of galactose, rhamnose, arabinose, and galacturonic acid. Furthermore, the st...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.2529

    authors： Monden T,Nakamura H,Murai A

    更新日期：1995-10-13 00:00:00

  • Demonstration of a Ca2+/calmodulin dependent protein kinase cascade in the hog heart.

    abstract：:Members of the Ca2+/calmodulin dependent protein kinase (CaMK) family and a CaMK cascade have been identified and well characterized in the brain, but little is known about their equivalents in the heart. Thus only CaMKII and its function have been reported so far. Therefore, we purified and characterized CaMKI and Ca...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.9154

    authors： Uemura A,Naito Y,Matsubara T,Hotta N,Hidaka H

    更新日期：1998-08-19 00:00:00

  • Paxillin localisation in osteocytes--is it determined by the direction of loading?

    abstract：:External mechanical loading of cells aligns cytoskeletal stress fibres in the direction of principle strains and localises paxillin to the mechanosensing region. If the osteocyte cell body can indeed directly sense matrix strains, then cytoskeletal alignment and distribution of paxillin in osteocytes in situ will bear...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.12.174

    authors： Vatsa A,Semeins CM,Smit TH,Klein-Nulend J

    更新日期：2008-12-26 00:00:00

  • Molecular cloning and sequence analysis of cDNA encoding human ferrochelatase.

    abstract：:The cDNA encoding human ferrochelatase [EC 4.99.1.1] was isolated from a human placenta cDNA library in bacteriophage lambda gt11 by screening with a radiolabeled fragment of mouse ferrochelatase cDNA. The cDNA had an open reading frame of 1269 base pairs (bp) encoding a protein of 423 amino acid residues (Mr. 47,833)...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)80099-3

    authors： Nakahashi Y,Taketani S,Okuda M,Inoue K,Tokunaga R

    更新日期：1990-12-14 00:00:00

  • Activated nuclear transcription factor kappaB in patients with myocarditis and dilated cardiomyopathy--relation to inflammation and cardiac function.

    abstract：OBJECTIVES AND BACKGROUND:Myocarditis is caused by various agents and autoimmune processes. It is unknown whether viral genome persistence represents inactive remnants of previous infections or whether it is attributed to ongoing adverse processes. The latter also applies to the course of autoimmune myocarditis. One pr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.10.195

    authors： Alter P,Rupp H,Maisch B

    更新日期：2006-01-06 00:00:00

  • DNA topoisomerase II from mammalian mitochondria is inhibited by the antitumor drugs, m-AMSA and VM-26.

    abstract：:A type II DNA topoisomerase has been partially purified from calf thymus mitochondria by a combination of differential centrifugation and column chromatography. The mitochondrial enzyme was inhibited by amsacrine (m-AMSA) slightly at 0.5 microM, significantly at 5.0 microM, and completely at 50 microM. A similar profi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)80239-6

    authors： Lin JH,Castora FJ

    更新日期：1991-04-30 00:00:00

  • Direct detection of SERCA calcium transport and small-molecule inhibition in giant unilamellar vesicles.

    abstract：:We have developed a charge-mediated fusion method to reconstitute the sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) in giant unilamellar vesicles (GUV). Intracellular Ca2+ transport by SERCA controls key processes in human cells such as proliferation, signaling, and contraction. Small-molecule effectors of SERCA are...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.10.096

    authors： Bian T,Autry JM,Casemore D,Li J,Thomas DD,He G,Xing C

    更新日期：2016-12-09 00:00:00

  • Serum anti-Ku86 is a potential biomarker for early detection of hepatitis C virus-related hepatocellular carcinoma.

    abstract：:Hepatocellular carcinoma (HCC), the predominant form of primary liver cancer, is one of the most common cancers worldwide and the third most common cause of cancer-related death. Imaging studies including ultrasound and computed tomography are recommended for early detection of HCC, but they are operator dependent, co...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.04.099

    authors： Nomura F,Sogawa K,Noda K,Seimiya M,Matsushita K,Miura T,Tomonaga T,Yoshitomi H,Imazeki F,Takizawa H,Mogushi K,Miyazaki M,Yokosuka O

    更新日期：2012-05-18 00:00:00

  • Ras proteins regulate multiple mitogenic pathways in A10 vascular smooth muscle cells.

    abstract：:We examined the role of ras proteins in mitogenesis and proliferation of A10 smooth muscle cells. An assay was developed in which a dominant negative ras gene could be transiently expressed in vascular smooth muscle cells. As opposed to cells transfected with a control plasmid, those transfected with a dominant negati...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.2065

    authors： Irani K,Herzlinger S,Finkel T

    更新日期：1994-08-15 00:00:00

  • Inactivation of the hMSH3 mismatch repair gene in bladder cancer.

    abstract：:Deficiency in the DNA mismatch repair (MMR) is frequently involved in various cancers. The hMSH3 gene is one of the human MMR genes whose role in bladder cancer is not known. We hypothesized that down-regulation of the hMSH3 gene might be involved in bladder cancer. In this study we analyzed this gene with regard to f...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.10.114

    authors： Kawakami T,Shiina H,Igawa M,Deguchi M,Nakajima K,Ogishima T,Tokizane T,Urakami S,Enokida H,Miura K,Ishii N,Kane CJ,Carroll PR,Dahiya R

    更新日期：2004-12-17 00:00:00

  • Biocatalysts: beautiful creatures.

    abstract：:The chemical industry has come under increasing pressure to make chemical production more eco-friendly and independent to fossil resources. The development of industrial processes based on micro-organisms can especially help to eliminate the use or the generation of hazardous substances and can support the transition ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章,评审

    doi：10.1016/j.bbrc.2012.07.028

    authors： Saibi W,Abdeljalil S,Masmoudi K,Gargouri A

    更新日期：2012-09-28 00:00:00

  • New method to differentiate human peripheral blood monocytes into insulin producing cells: Human hematosphere culture.

    abstract：:Strategy to differentiate stem cells into insulin producing cells (IPCs) in vitro has been a promising one to get cell source of β-cell replacement therapy for diabetes. It has been suggested that islets and neurons share features and nestin-positive cells could differentiate into IPCs. We have recently developed a th...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.01.096

    authors： Hur J,Yang JM,Choi JI,Yun JY,Jang JH,Kim J,Kim JY,Oh IY,Yoon CH,Cho HJ,Park YB,Kim HS

    更新日期：2012-02-24 00:00:00

  • The distribution of glycosyl-phosphatidylinositol anchored proteins is differentially regulated by serum and insulin.

    abstract：:Glycosyl-phosphatidylinositol (GPI) anchored proteins are surveyed in two insulin sensitive cell types by surface labeling and phospholipase C-induced release into the medium. Serum starvation selectively increases both the number and intensity of a subset of GPI-anchored proteins. After serum starvation, loss of cell...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)91533-7

    authors： Lisanti MP,Darnell JC,Chan BL,Rodriguez-Boulan E,Saltiel AR

    更新日期：1989-10-31 00:00:00

  • 3D model of human arylamine N-acetyltransferase 2: structural basis of the slow acetylator phenotype of the R64Q variant and analysis of the active-site loop.

    abstract：:The human arylamine N-acetyltransferase NAT2 is responsible for the biotransformation of numerous arylamine drugs and carcinogens. A common polymorphism of the NAT2 gene has been associated with susceptibility to drug toxicity and various malignancies. In this study, we used the crystal structure of the Salmonella typ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2002.6414

    authors： Rodrigues-Lima F,Dupret JM

    更新日期：2002-02-15 00:00:00

  • Local database and the search program for proteomic analysis of sperm proteins in the ascidian Ciona intestinalis.

    abstract：:Separation of proteins by two-dimensional electrophoresis and following mass spectrometry (MS) is now a conventional technique for proteomic analysis. For proteomic analysis of a certain tissue with a limited information of primary structures of proteins, we have developed an analytical system for peptide mass fingerp...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.05.118

    authors： Hozumi A,Satouh Y,Ishibe D,Kaizu M,Konno A,Ushimaru Y,Toda T,Inaba K

    更新日期：2004-07-09 00:00:00

  • Real-time monitoring of RNA helicase activity using fluorescence resonance energy transfer in vitro.

    abstract：:We have developed a continuous fluorescence assay based on fluorescence resonance energy transfer (FRET) for the monitoring of RNA helicase activity in vitro. The assay is tested using the hepatitis C virus (HCV) NS3 helicase as a model. We prepared a double-stranded RNA (dsRNA) substrate with a 5' fluorophore-labeled...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.01.100

    authors： Tani H,Fujita O,Furuta A,Matsuda Y,Miyata R,Akimitsu N,Tanaka J,Tsuneda S,Sekiguchi Y,Noda N

    更新日期：2010-02-26 00:00:00

  • Molecular phylogeny of C1 inhibitor depicts two immunoglobulin-like domains fusion in fishes and ray-finned fishes specific intron insertion after separation from zebrafish.

    abstract：:C1 inhibitor (C1IN) is a multi-facet serine protease inhibitor in the plasma cascades, inhibiting several proteases, notably, regulates both complement and contact system activation. Despite huge advancements in the understanding of C1IN based on biochemical properties and its roles in the plasma cascades, the phyloge...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.05.097

    authors： Kumar A,Bhandari A,Sarde SJ,Goswami C

    更新日期：2014-07-18 00:00:00

  • In vivo regulation of human CrkII by cyclophilin A and FK506-binding protein.

    abstract：:Members of the Crk family of adaptor proteins are key players in signal transduction from a variety of cell surface receptors. CrkI and CrkII are two alternative-spliced forms of a single gene which possess an N-terminal SH2 domain and an SH3 domain that mediate interaction with other proteins. CrkII possesses an addi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.01.027

    authors： Nath PR,Dong G,Braiman A,Isakov N

    更新日期：2016-02-05 00:00:00

  • Unusual RNA polymerase content of Trypanosoma brucei nuclei.

    abstract：:Nuclei were isolated from bloodstream forms of Trypanosoma brucei by nitrogen cavitation and sedimentation through percoll density gradients. Transcription studies with these nuclei in vitro demonstrated features not seen with other eukaryotes: RNA synthesis was much greater in the presence of Mn2+ than with Mg2+ and ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(85)91316-6

    authors： Earnshaw DL,Beebee TJ,Gutteridge WE

    更新日期：1985-09-16 00:00:00

  • MicroRNA-340 inhibits the proliferation and invasion of hepatocellular carcinoma cells by targeting JAK1.

    abstract：:Increasing evidence indicates that dysregulation of microRNAs (miRNAs) contributes to tumorigenesis. MicroRNA-340 (miR-340) is downregulated in several types of cancer. However, the functional mechanism of miR-340 in hepatocellular carcinoma (HCC) remains unclear. Here, we showed that miR-340 was significantly downreg...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.12.102

    authors： Yuan J,Ji H,Xiao F,Lin Z,Zhao X,Wang Z,Zhao J,Lu J

    更新日期：2017-01-29 00:00:00

  • ATR microspectroscopy with multivariate analysis segregates grades of exfoliative cervical cytology.

    abstract：:Although cervical cancer screening in the UK has led to reductions in the incidence of invasive disease, this programme remains flawed. We set out to examine the potential of infrared (IR) microspectroscopy to allow the profiling of cellular biochemical constituents associated with disease progression. Attenuated tota...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.11.005

    authors： Walsh MJ,Singh MN,Pollock HM,Cooper LJ,German MJ,Stringfellow HF,Fullwood NJ,Paraskevaidis E,Martin-Hirsch PL,Martin FL

    更新日期：2007-01-05 00:00:00

  • argJ mutations are highly inducible by ethidium bromide in proB strains of Streptomyces lividans: implication of pathway interactions.

    abstract：:Spontaneous Arg- mutants arose at high frequencies in Streptomyces lividans. Exposure to ethidium bromide increased the frequency of arg instability. In Pro+ strains the induced arg mutants were mainly argG, but in the proB mutants, a new mutation, argJ, prevailed which lacked ornithine acetyltransferase activity and ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(92)92318-r

    authors： Chou CF,Chen CW

    更新日期：1992-12-15 00:00:00