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New method to differentiate human peripheral blood monocytes into insulin producing cells: Human hematosphere culture.

Abstract：

:Strategy to differentiate stem cells into insulin producing cells (IPCs) in vitro has been a promising one to get cell source of β-cell replacement therapy for diabetes. It has been suggested that islets and neurons share features and nestin-positive cells could differentiate into IPCs. We have recently developed a three-dimensional culture system using human peripheral blood cells named as blood-born hematosphere (BBHS). Here we showed that most of BBHS were composed of nestin-positive cells. Under the four-stage differentiation protocol for IPCs, we plated nestin-positive BBHS onto fibronectin-coated dish. These cells form islet-like clusters and most of them expressed insulin. Pancreatic specific genes were turned on, such as transcription factors (Pdx-1, Ngn3 and Nkx6.1), genes related to endocrine function (Glut-2 and PC2) or β cell function (Kir6.2, SUR1). Furthermore islet differentiation was confirmed by dithizone (DTZ) staining to detect zinc ion which binds insulin protein within the cells. Finally, IPCs derived from BBHS showed capability to secrete insulin in response to glucose stimulation. Taken together, our novel protocol successfully induced islet-like human insulin producing cells out of BBHS. This strategy of ex vivo expansion of IPCs using BBHS provides an autologous therapeutic cell source for the treatment of diabetes.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Hur J,Yang JM,Choi JI,Yun JY,Jang JH,Kim J,Kim JY,Oh IY,Yoon CH,Cho HJ,Park YB,Kim HS

doi

10.1016/j.bbrc.2012.01.096

subject

Has Abstract

pub_date

2012-02-24 00:00:00

pages

765-9

issue

4

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(12)00143-X

journal_volume

418

pub_type

杂志文章

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