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Efficient gene transduction by Epstein-Barr-virus-based vectors coupled with cationic liposome and HVJ-liposome.

Abstract：

:We show here a novel non-viral strategy to transduce human cells by using an EBV-based vector system. The EBV-based vectors, the plasmid vectors carrying EBV oriP (origin for plasmid replication) and EBNA (EBV nuclear antigen) 1 gene from EBV genome, were combined with 2 gene delivery systems, i.e., cationic liposome and HVJ-liposome. By both methods, EBV-based vectors could be more efficiently transfected into HeLa cells than non-EBV, conventional plasmid vectors. When human primary fibroblasts were transfected, EBV-based vectors coupled with cationic liposome but HVJ-liposome resulted in successful gene transduction, while human bone marrow cells were transduced with both HVJ-liposome- and cationic liposome-EBV vectors. These results suggest the potential applications of the EBV-based vector system for gene therapy.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Satoh E,Osawa M,Tomiyasu K,Hirai H,Shimazaki C,Oda Y,Nakagawa M,Kondo M,Kinoshita S,Mazda O,Imanishi J

doi

10.1006/bbrc.1997.7060

subject

Has Abstract

pub_date

1997-09-29 00:00:00

pages

795-9

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006291X97970601

journal_volume

238

pub_type

杂志文章

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