Heterologous expression of active manganese peroxidase from Phanerochaete chrysosporium using the baculovirus expression system.

abstract：:The effects of trace acetylation on the contact sites in peptide-antibody binding have been investigated by using a label selection method. A 13-residue synthetic peptide having three lysines (LKLVEQQNPKVKL) corresponding to sequence position 155-168 of beta 1,4-galactosyltransferase was used in this study. The four a...

journal_title：Biochemical and biophysical research communications

authors： Yadav SP,Rawitch AB

更新日期：1994-12-30 00:00:00

abstract：:Parkinson's disease (PD) is one of the most prevalent neurodegenerative diseases but its etiology is unclear. Alpha-synuclein (alpha-SN) is a major component of Lewy bodies and Lewy neurites, and its missense mutations, A30P and A53T, cause familial PD. In PD, alpha-SN-positive glial inclusions are distributed mainly ...

journal_title：Biochemical and biophysical research communications

authors： Kohno R,Sawada H,Kawamoto Y,Uemura K,Shibasaki H,Shimohama S

更新日期：2004-05-21 00:00:00

abstract：:Aegyptin is a mosquito salivary gland protein and potent inhibitor of platelet aggregation. Aegyptin binds to the von Willebrand factor-binding site on collagen and prevents its interaction with platelets. Because collagen also induces plasma clotting by activation of factor XII, we evaluated the effects of aegyptin o...

journal_title：Biochemical and biophysical research communications

authors： Mizurini DM,Francischetti IM,Monteiro RQ

更新日期：2013-06-28 00:00:00

abstract：:Hepatitis delta virus (HDV) is a human pathogen causing fulminant hepatitis and liver cirrhosis. HDV has a circular single-stranded RNA genome, which encodes only a single protein, hepatitis delta antigen (HDAg), from the antigenomic strand. Although the functional roles of HDAg have been extensively studied, the mole...

journal_title：Biochemical and biophysical research communications

authors： Hwang SB,Park KJ,Kim YS

更新日期：1998-03-27 00:00:00

abstract：:Liver regeneration is controlled by multiple signaling pathways induced by a variety of growth factors, hormones, and cytokines. Here we report that protein kinase B (PKB)/Akt, part of a key cell survival signaling pathway, is markedly activated after partial hepatectomy (PHX). The antiapoptotic protein Bad, a downstr...

journal_title：Biochemical and biophysical research communications

authors： Hong F,Nguyen VA,Shen X,Kunos G,Gao B

更新日期：2000-12-29 00:00:00

abstract：:Directed neural differentiation of human embryonic stem cells (ESCs) enables researchers to generate diverse neuronal populations for human neural development study and cell replacement therapy. To realize this potential, it is critical to precisely understand the role of various endogenous and exogenous factors invol...

journal_title：Biochemical and biophysical research communications

authors： Liu C,Sun Y,Arnold J,Lu B,Guo S

更新日期：2014-10-03 00:00:00

abstract：:The Z-DNA binding domain of human ADAR1 (Zα(ADAR1)) preferentially binds Z-DNA rather than B-DNA with high binding affinity. Here, we have carried out chemical shift perturbation and backbone dynamics studies of Zα(ADAR1) in the free form and in complex with three DNA duplexes, d(CGCGCG)(2), d(CACGTG)(2), and d(CGTACG...

journal_title：Biochemical and biophysical research communications

authors： Lee AR,Kim HE,Lee YM,Jeong M,Choi KH,Park JW,Choi YG,Ahn HC,Choi BS,Lee JH

更新日期：2012-11-09 00:00:00

abstract：:Monosomy 7 and interstitial deletions in the long arm of chromosome 7 (-7/7q-) is a common nonrandom chromosomal abnormality found frequently in myeloid disorders including acute myeloid leukemia (AML), myelodysplastic syndrome (MDS), and juvenile myelomonocytic leukemia (JMML). Using a short probe-based microarray co...

journal_title：Biochemical and biophysical research communications

authors： Asou H,Matsui H,Ozaki Y,Nagamachi A,Nakamura M,Aki D,Inaba T

更新日期：2009-05-29 00:00:00

abstract：:Hexokinase II protein is augmented in denervated skeletal muscle; therefore, we determined if hexokinase II gene transcription rates and mRNA levels are increased with denervation. The right hindlimb skeletal muscles of male rats were denervated while the left hindlimbs were sham operated. Seventy-two h following surg...

journal_title：Biochemical and biophysical research communications

authors： Jones JP,Roberts BR,Tapscott EB,Dohm GL

更新日期：1997-09-08 00:00:00

abstract：:An expression library established in lambda gt11 with cDNA from squamous epithelium of the human upper digestive tract was screened with an antibody raised against keratin 13. A 1.2 kb fragment from the most strongly reacting plaque was sequenced and compared to known type I keratin sequences. The highest degree of ho...

journal_title：Biochemical and biophysical research communications

authors： Schulz P,Wachter E,Hochstrasser K,Wild AG,Mischke D

更新日期：1989-08-15 00:00:00

abstract：:Protein insolubility is a major problem when producing recombinant proteins (e.g., to be used as antigens) from large cDNAs in Escherichia coli. Here, we describe a system using three convertible plasmid vectors to screen for soluble proteins produced in E. coli. This system experimentally identified any random cDNA f...

journal_title：Biochemical and biophysical research communications

authors： Nakayama M,Ohara O

更新日期：2003-12-19 00:00:00

abstract：:Cholecystokinin (CCK) dose-dependently stimulates enzyme secretion or loss of cell integrity in the exocrine pancreas. Signaling mechanims include tyrosine phosphorylation of p125(FAK) and paxillin. Here, we examine their potential function. Maximum phosphorylation of both proteins was observed after stimulation of fr...

journal_title：Biochemical and biophysical research communications

authors： Leser J,Lührs H,Beil MF,Adler G,Lutz MP

更新日期：1999-01-19 00:00:00

abstract：:Heat shock proteins (HSPs) are involved in protein folding, aggregation, transport and/or stabilization by acting as a molecular chaperone, leading to inhibition of apoptosis by both caspase dependent and/or independent pathways. HSPs are overexpressed in a wide range of human cancers and are implicated in tumor cell ...

journal_title：Biochemical and biophysical research communications

authors： Sarkar R,Mukherjee S,Biswas J,Roy M

更新日期：2012-10-12 00:00:00

abstract：:There is increasing evidence for the presence of cancer stem-like progenitors in malignant brain tumors. This subpopulation of progenitor cells, the so-called "cancer stem cells (CSCs)", may play a pivotal role in brain tumor initiation, growth, and recurrence. Here we describe the establishment of one permanent brain...

journal_title：Biochemical and biophysical research communications

authors： Inagaki A,Soeda A,Oka N,Kitajima H,Nakagawa J,Motohashi T,Kunisada T,Iwama T

更新日期：2007-09-28 00:00:00

abstract：:The transcription of human nm23 genes (nm23-H1, nm23-H2) is involved in suppression of tumor metastasis or tumor progression. Therefore the characterization of transcriptional regulatory mechanisms for both nm23 genes is very important. In this study we have isolated and analyzed the 5'-flanking region of the human nm...

journal_title：Biochemical and biophysical research communications

authors： Seifert M,Seib T,Engel M,Dooley S,Welter C

更新日期：1995-10-24 00:00:00

abstract：:Little is known about determinants regulating expression of Mannan-binding lectin associated serine protease-2 (MASP-2), the effector component of the lectin pathway of complement activation. Comparative bioinformatic analysis of the MASP2 promoter regions in human, mouse, and rat, revealed conservation of two putativ...

journal_title：Biochemical and biophysical research communications

authors： Unterberger C,Hanson S,Klingenhoff A,Oesterle D,Frankenberger M,Endo Y,Matsushita M,Fujita T,Schwaeble W,Weiss EH,Ziegler-Heitbrock L,Stover C

更新日期：2007-12-28 00:00:00

abstract：:ZinT (B1973), previously known as YodA, was originally characterised as a cadmium-induced periplasmic protein under the regulation of Fur and SoxS. Here we describe a decrease in zinT transcript in response to elevated copper concentrations and the zinc and copper dependent phenotype of a DeltazinT strain. Cadmium sen...

journal_title：Biochemical and biophysical research communications

authors： Kershaw CJ,Brown NL,Hobman JL

更新日期：2007-12-07 00:00:00

abstract：:Genes for the human vacuolar type H(+)-ATPase proteolipid (16-kDa) subunit were cloned and their nucleotide sequences were determined. Comparison of the deduced sequences indicated that at least four genes including pseudogenes are present in the human genome. One of them corresponded to that for the 16-kDa subunit ex...

journal_title：Biochemical and biophysical research communications

authors： Hasebe M,Hanada H,Moriyama Y,Maeda M,Futai M

更新日期：1992-03-16 00:00:00

abstract：:Autophagy has been associated with a variety of diseases especially aging. Human dermal fibroblasts (HDFs) can internalize and then degrade elastin, collagen and advanced glycation end products (AGEs) in lysosomes, which plays prominent roles in extracellular matrix homeostasis and AGEs removal in the dermis. Although...

journal_title：Biochemical and biophysical research communications

authors： Huang Y,Li Y,Qu Y,Zheng Y,Ouyang M,Zhang Y,Lai W,Xu Q

更新日期：2019-10-22 00:00:00

abstract：:Brewers yeast contains factors that increase and decrease glucose tolerance. Hop components (lupulones) that adhere to yeast during the brewing process elicit a variety of biological effects including the induction of hepatic cytochrome P4503A. Colupulone was tested for its effects on glucose tolerance and cytochrome ...

journal_title：Biochemical and biophysical research communications

authors： Mannering GJ,Shoeman JA,Shoeman DW

更新日期：1994-05-16 00:00:00

abstract：:Recent years have seen considerable evolution in our understanding of the mechanisms of oxygen activation by non-heme iron enzymes, with high-valent iron-oxo intermediates coming to the forefront as formidably potent oxidants. In the absence of substrate, the generation of vividly colored chromophores deriving from th...

journal_title：Biochemical and biophysical research communications

authors： Farquhar ER,Koehntop KD,Emerson JP,Que L Jr

更新日期：2005-12-09 00:00:00

abstract：:Expression of macrophage paraoxonase 2 (PON2), a cellular lactonase with anti-oxidant and anti-atherogenic properties, was shown to be upregulated under high oxidative stress. The aim of the present study was to analyze the relationship between the extent of cellular oxidative stress in J774A.1 macrophage and PON2 lac...

journal_title：Biochemical and biophysical research communications

authors： Shiner M,Fuhrman B,Aviram M

更新日期：2006-10-27 00:00:00

abstract：:L-arginine may aid in the liver detoxification and may benefit in the treatment of liver disorders such as liver injury. The present study was to investigate the possible protective and curative effects of L-arginine on carbon tetrachloride (CCl(4)) induced hepatotoxicity. Mice received a single dose of CCl(4). L-argi...

journal_title：Biochemical and biophysical research communications

authors： Saad EA

更新日期：2012-06-22 00:00:00

abstract：:While there have been many single-molecule studies of kinesin-1, most have been done along microtubules purified from bovine or porcine brain, and relatively little is known about how variations in tubulin might alter motor function. Of particular interest is transport along microtubules polymerized from tubulin purif...

journal_title：Biochemical and biophysical research communications

authors： Feizabadi MS,Jun Y

更新日期：2014-11-28 00:00:00

abstract：:Hyperphosphorylation of extracellular signal-regulated protein kinases-1/2 (ERK1/2) is known to promote cancer cell proliferation. We therefore investigated the constitutive phosphorylation levels of ERK1/2 and the expression of its downstream targets c-Fos, c-Jun, and cyclooxygenase-2 (COX-2) in biopsied human gastri...

journal_title：Biochemical and biophysical research communications

authors： Wu WK,Sung JJ,Yu L,Li ZJ,Chu KM,Cho CH

更新日期：2008-08-22 00:00:00

abstract：:Estrogen receptor alpha (ERα) is a nuclear receptor that regulates a range of physiological processes in response to estrogens. In order to study its biological role, we generated a floxed ERα mouse line that can be used to knock out ERα in selected tissues by using the Cre/LoxP system. In this study, we established a...

journal_title：Biochemical and biophysical research communications

authors： Antonson P,Omoto Y,Humire P,Gustafsson JÅ

更新日期：2012-08-10 00:00:00

abstract：:Chemotaxis of rabbit peritoneal leucocytes stimulated by fMet-Leu-Phe, a synthetic chemoattractant, was inhibited by Glu-Glu-Glu-Glu-Tyr-Pro-Met-Glu (MT peptide) and Leu-Ile-Glu-Asp-Asn-Glu-Tyr-Thr-Ala-Arg-Glu-Gly (Src peptide). Both peptides did not inhibit the binding of [3H] formyl-NLe-Leu-Phe, a chemoattractant, t...

journal_title：Biochemical and biophysical research communications

authors： Hirata F,Notsu Y,Matsuda K,Vasanthakumar G,Schiffmann E,Wong TW,Goldberg AR

更新日期：1984-01-30 00:00:00

abstract：:The purpose of this study was to determine the effects of dietary fatty acids on intestinal apolipoprotein (apo) A-IV and C-III expression in the newborn piglet. Two-day-old female piglets received one of three isocaloric formulas containing medium-chain triglycerides (TG) (MCT), intermediate-chain saturated TG (ICST)...

journal_title：Biochemical and biophysical research communications

authors： Black DD,Wang H,Hunter F,Zhan R

更新日期：1996-04-25 00:00:00

abstract：:Ap4A hydrolase (asymmetrical diadenosine tetraphosphate hydrolase, EC 3.6.1.17), an enzyme involved in a number of biological processes, is characterized as cleaving the polyphosphate chain at the fourth phosphate from the bound adenosine moiety. This paper presents the crystal structure of wild-type and E58A mutant h...

journal_title：Biochemical and biophysical research communications

authors： Ge H,Chen X,Yang W,Niu L,Teng M

更新日期：2013-03-01 00:00:00

abstract：:MicroRNAs play important roles in the carcinogenesis of many types of cancers by inhibiting gene expression at posttranscriptional level. However, the roles of microRNAs in hepatocellular carcinoma, are still unclear. Here, we identified that miR-367 promotes hepatocellular carcinoma (HCC) cell proliferation by negati...

journal_title：Biochemical and biophysical research communications

authors： Meng X,Lu P,Fan Q

更新日期：2016-01-29 00:00:00