Abstract:
:There is increasing evidence for the presence of cancer stem-like progenitors in malignant brain tumors. This subpopulation of progenitor cells, the so-called "cancer stem cells (CSCs)", may play a pivotal role in brain tumor initiation, growth, and recurrence. Here we describe the establishment of one permanent brain tumor stem cell line that able to form new spheres after culture under adherent monolayer conditions and to recapitulate the properties of the original tumor upon transplantation into immunodeficient mice. Re-formed spheres retained their stem cell properties and isolated single CSCs from these spheres formed spheres/tumors even after long-term cultures (over 2 years). These data suggested that a small population of CSCs preserved its stem cell properties even after serial passages under non-adherent/adherent culture conditions. Evaluation of underlying metabolic events and assessment of the biological features of these viable cell lines will yield useful knowledge on the in situ behavior of brain tumors.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Inagaki A,Soeda A,Oka N,Kitajima H,Nakagawa J,Motohashi T,Kunisada T,Iwama Tdoi
10.1016/j.bbrc.2007.07.037subject
Has Abstractpub_date
2007-09-28 00:00:00pages
586-92issue
3eissn
0006-291Xissn
1090-2104pii
S0006-291X(07)01490-8journal_volume
361pub_type
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