Cell cycle synchronization of embryonic stem cells: effect of serum deprivation on the differentiation of embryonic bodies in vitro.

abstract：:Little is known about the gene expression in human oocytes and early embryo development because of the rare availability of the materials. The recent advancement of biotechnology has allowed one to analyze the gene expression in single human oocytes and preimplantation embryos. Gene expression of human lactate dehydro...

journal_title：Biochemical and biophysical research communications

authors： Li SS,Liu YH,Tseng CN,Singh S

更新日期：2006-02-03 00:00:00

abstract：:The intrinsic fluorescence properties of human alpha apohemoglobin at protein concentrations from 1 to 5 microM in 0.1 M potassium phosphate buffer, pH 7 or 8 at 5 degrees C were monitored in the absence and presence of a fixed concentration (5 microM) of a fluorescence quenching heme-containing native or Des (146-His...

journal_title：Biochemical and biophysical research communications

authors： Chiu F,Vasudevan G,Morris A,McDonald MJ

更新日期：1998-01-14 00:00:00

abstract：:Cells of the monocyte series respond to follicle stimulating hormone (FSH) by poorly characterized mechanisms. We studied FSH-receptors (FSH-R) and FSH response in nontransformed human monocytes and in osteoclasts differentiated from these cells. Western blot and PCR confirmed FSH-R expression on monocytes or osteocla...

journal_title：Biochemical and biophysical research communications

authors： Robinson LJ,Tourkova I,Wang Y,Sharrow AC,Landau MS,Yaroslavskiy BB,Sun L,Zaidi M,Blair HC

更新日期：2010-03-26 00:00:00

abstract：:Dengue Virus (DENV) infection is an important mosquito-borne viral disease and its clinical symptoms range from a predominantly febrile disease, dengue fever (DF), to dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). Increased levels of cytokines - the so-called 'cytokine storm', contribute to the pathog...

journal_title：Biochemical and biophysical research communications

authors： Suttitheptumrong A,Khunchai S,Panaampon J,Yasamut U,Morchang A,Puttikhunt C,Noisakran S,Haegeman G,Yenchitsomanus PT,Limjindaporn T

更新日期：2013-06-28 00:00:00

abstract：:Insulin and glucagon secretion from the islets of Langerhans is highly regulated. Although an increased plasma glucose level is the major stimulus for insulin exocytosis, roles for glutamine and glutamate have been suggested. Interestingly, the islet cells display elements associated with synaptic transmission. In the...

journal_title：Biochemical and biophysical research communications

authors： Gammelsaeter R,Jenstad M,Bredahl MK,Gundersen V,Chaudhry FA

更新日期：2009-04-10 00:00:00

abstract：:Hypothalamic peptides, gonadotropin-releasing hormone (GnRH) and gonadotropin inhibitory hormone (GnIH), play pivotal roles in the control of reproduction and gonadal maturation in fish. In the present study we tested the possibility that stress-mediated reproductive dysfunction in teleost may involve changes in GnRH ...

journal_title：Biochemical and biophysical research communications

authors： Choi YJ,Habibi HR,Kil GS,Jung MM,Choi CY

更新日期：2017-04-01 00:00:00

abstract：:Pathways formed of delocalized π-electron systems and polar groups of polypeptide chains bridged by hydrogen bonds are referred as π-H pathways. Suitable for electron transfer, these pathways in cytochrome c oxidase connect CuA, the source of electrons distributed in cytochrome c oxidase, with the metal centers, heme ...

journal_title：Biochemical and biophysical research communications

authors： Ramasarma T,Vaigundan D

更新日期：2019-03-05 00:00:00

abstract：:We previously found that p53 binds to the catalytic subunit of the p34(cdc2)/cyclin B1-kinase. In the present study we analyzed the functional consequences of this interaction. Binding of wild-type p53 to p34(cdc2)/cyclin B1 results in a significant decrease of its histone H1 kinase activity. Binding of p53 to the kin...

journal_title：Biochemical and biophysical research communications

authors： Ababneh M,Götz C,Montenarh M

更新日期：2001-05-04 00:00:00

abstract：:Human thyroperoxidase (TPO) ectodomain is successively made of myeloperoxidase-, complement control protein repeat-, and epidermal growth factor-like gene modules. However, the TPO immunodominant region targeted by autoantibodies from patients with an autoimmune thyroid disease has not been mapped on the molecule. Her...

journal_title：Biochemical and biophysical research communications

authors： Blanchin S,Estienne V,Guo J,Rapoport B,McLachlan SM,Carayon P,Ruf J

更新日期：2002-08-02 00:00:00

abstract：:The ATP-sensitive potassium channel of insulin-secreting pancreatic beta-cells is a complex of Kir6.2, a member of the inwardly rectifying potassium channel superfamily, and the sulfonylurea receptor. We have isolated cDNA clones encoding rat Kir6.2. Co-expression of rat Kir6.2 and sulfonylurea receptor in human embry...

journal_title：Biochemical and biophysical research communications

authors： Tokuyama Y,Fan Z,Furuta H,Makielski JC,Polonsky KS,Bell GI,Yano H

更新日期：1996-03-27 00:00:00

abstract：:Neutrophils stimulated with a low concentration of chemotactic peptide N-formyl-met-leu-phe respond with a brief pulse of actin polymerization and a persistent change in morphology from round to polarized. The recovery of the actin polymerization response is unlikely to be due to usual receptor desensitization mechani...

journal_title：Biochemical and biophysical research communications

authors： Model MA,Omann GM

更新日期：1996-07-16 00:00:00

abstract：:Calcitonin gene-related peptide (CGRP) in porcine ventricular muscles. positive inotropic effects in the isolated, electrically driven false tendon of the porcine heart. Specific CGRP-binding sites were present in solubilized membrane fractions; the dissociation constant (Kd) and the maximum binding (Bmax) were 50.4 p...

journal_title：Biochemical and biophysical research communications

authors： Miyauchi T,Sano Y,Hiroshima O,Yuzuriha T,Sugishita Y,Ishikawa T,Saito A,Goto K

更新日期：1988-08-30 00:00:00

abstract：:BRCA1-BRCA2-containing complex subunit 3 (BRCC3), a Lys-63-specific deubiquitinase, is a member of the JAMM/MPN family of zinc metalloproteases. BRCC3 have been shown to promote the inflammasome activation by deubiquitinating NOD-like receptor containing pyrin domain 3 (NLRP3). We reported the involvement of neuronal ...

journal_title：Biochemical and biophysical research communications

authors： Cheng X,Xu S,Zhang C,Qin K,Yan J,Shao X

更新日期：2020-02-12 00:00:00

abstract：:In the present study we show that neutral hexanoyl-(glyco)sphingolipids inhibit P-glycoprotein (Pgp) activity in human ovarian 2780AD cells. By contrast, hexanoylceramide and the gangliosides GM(3) and GM(2) had no effect on Pgp activity, whereas sphingosine had a stimulating effect. In the case of hexanoylglucosylcer...

journal_title：Biochemical and biophysical research communications

authors： Veldman RJ,Sietsma H,Klappe K,Hoekstra D,Kok JW

更新日期：1999-12-20 00:00:00

abstract：:Many amplified-chromosome regions in human cancers have been identified, while there are only a limited number of reports for amplified genes in a single amplicon. We recently isolated four cDNA clones, A39, B47, C51, and CAB1, from 500 kilobases of yeast artificial chromosome DNA containing the c-ERBB-2 gene. B47 con...

journal_title：Biochemical and biophysical research communications

authors： Kishi T,Sasaki H,Akiyama N,Ishizuka T,Sakamoto H,Aizawa S,Sugimura T,Terada M

更新日期：1997-03-06 00:00:00

abstract：:By using a radioimmunoassay (RIA) system newly established for human brain natriuretic peptide (BNP), a high concentration of immunoreactive (ir-) human BNP (hBNP) has been found in cardiac atrium (1). Two molecular forms of ir-hBNP of 4K and 13-15K were isolated from atrial extracts by using anti-hBNP IgG immunoaffin...

journal_title：Biochemical and biophysical research communications

authors： Hino J,Tateyama H,Minamino N,Kangawa K,Matsuo H

更新日期：1990-03-16 00:00:00

abstract：:The conversion of the endogenous cellular prion protein to an abnormally folded isoform is a hallmark of transmissible spongiform encephalopathies. It occurs when a misfolded prion protein contacts the cellular PrP. Among the molecular partners suggested to be involved in the misfolding process, the glycosaminoglycans...

journal_title：Biochemical and biophysical research communications

authors： Martin R,Chantepie S,Chapuis J,Le-Duc A,Maftah A,Papy-Garcia D,Laude H,Petit JM,Gallet PF

更新日期：2011-10-28 00:00:00

abstract：:We explored the role of the recently discovered "early growth response gene-1 (Egr-1)" in the induction of myocardial protein synthesis by endothelin-1. Endothelin-1 stimulated protein synthesis (i.e. 3H-phenylalanine incorporation) in isolated adult rat cardiomyocytes more than 2-fold. Addition of a 15mer Egr-1 antis...

journal_title：Biochemical and biophysical research communications

authors： Neyses L,Nouskas J,Vetter H

更新日期：1991-11-27 00:00:00

abstract：:Neurons in the adult central nervous system (CNS) have a poor intrinsic axon growth potential after injury, but the underlying mechanisms are largely unknown. Wingless-related mouse mammary tumor virus integration site (WNT) family members regulate neural stem cell proliferation, axon tract and forebrain development i...

journal_title：Biochemical and biophysical research communications

authors： Duan RS,Liu PP,Xi F,Wang WH,Tang GB,Wang RY,Saijilafu,Liu CM

更新日期：2018-05-05 00:00:00

abstract：:Liver kinase B1 (LKB1), a tumour suppressor, participates in many cellular processes, including cell survival, growth, apoptosis, transformation, and metabolism. Upon performing yeast two-hybrid screening, co-immunoprecipitation, and GST pull-down, we identified that BRCA1-associated protein 1 (BAP1), a deubiquitinase...

journal_title：Biochemical and biophysical research communications

authors： Yang C,Ding H,Yang Y,Yang L,Yang Y,Fang M,Ren J,Hu R,Wang C,Geng W

更新日期：2020-09-03 00:00:00

abstract：:Genes are organized as chromatin domains and positioned in the nucleus through regions of nuclear matrix association, termed MARs. Although well-studied, the mechanisms regulating expression of the beta-globin locus remain an enigma. Here, we show that certain MAR sequences of the beta-globin locus are conserved and r...

journal_title：Biochemical and biophysical research communications

authors： Walter WR,Singh GB,Krawetz SA

更新日期：1998-01-14 00:00:00

abstract：:A secondary structure has been predicted for the hemorrhagic metalloproteases using a method developed in Zurich that extracts structural information from patterns of conservation and variation in homologous protein sequences. This prediction tests the limits of the method when applied to a small number of homologous ...

journal_title：Biochemical and biophysical research communications

authors： Gerloff DL,Jenny TF,Knecht LJ,Benner SA

更新日期：1993-07-15 00:00:00

abstract：:Molecular chaperones prevent the aggregation of partially folded or misfolded forms of protein. alpha-crystallin performs such a function in the ocular lens. To gain insight into the mechanism of the anti-aggregation activity of alpha-crystallin, we performed dynamic light scattering (DLS) measurements investigating i...

journal_title：Biochemical and biophysical research communications

authors： Bettelheim FA,Ansari R,Cheng QF,Zigler JS Jr

更新日期：1999-08-02 00:00:00

abstract：:An expression vector encoding a chimeric envelope protein composed of CD4 and ecotropic retroviral envelope glycoprotein was constructed with the aim of accomplishing targeted gene transfer into HIV-1-infected cells. The chimeric protein was efficiently expressed and transported to the surfaces of various cell types a...

journal_title：Biochemical and biophysical research communications

authors： Suzuki S,Shimada T

更新日期：2000-05-19 00:00:00

abstract：:The human pancreas carcinoma cell line T3M-4 contains activated c-Kirsten (K)-ras oncogene detectable by the DNA-mediated gene transfer technique using NIH/3T3 cells. DNA fragments containing coding lesions have been cloned, and nucleotide sequence analysis suggests that the T3M-4 oncogene has been activated by a sing...

journal_title：Biochemical and biophysical research communications

authors： Hirai H,Okabe T,Anraku Y,Fujisawa M,Urabe A,Takaku F

更新日期：1985-02-28 00:00:00

abstract：:Incubation of rabbit aortic rings with interleukin-1 (100 U/ml) in vitro led to a depressed contractile response to norepinephrine, whether the endothelium was present or not. In both cases norepinephrine-induced contraction was restored in the presence of NG-methyl-L-arginine (300 microM), an inhibitor of nitric oxid...

journal_title：Biochemical and biophysical research communications

authors： Robert R,Chapelain B,Jean T,Néliat G

更新日期：1992-01-31 00:00:00

abstract：:Myogenesis is regulated by the basic helix-loop-helix (bHLH) myogenic regulatory factor MyoD, which induces muscle-specific gene expression by binding to the E-box sequence as a heterodimer with ubiquitous bHLH E2A (E12/E47) proteins. Here, we report that a 31-kDa caspase-generated cleavage product of Crk-associated s...

journal_title：Biochemical and biophysical research communications

authors： Jeong DE,Lee EK,Song WK,Kim W

更新日期：2014-02-21 00:00:00

abstract：:Treatment of quiescent Balb/c 3T3 cells with insulin-like growth factor I (IGF I) resulted in the stimulation of proto-oncogene c-jun transcription. Cells exposed to cycloheximide and IGF I together showed super-induction of c-jun transcripts. Nuclear run-off assay revealed that IGF I up-regulated c-jun only while cyc...

journal_title：Biochemical and biophysical research communications

authors： Chiou ST,Chang WC

更新日期：1992-03-16 00:00:00

abstract：:Irradiation induces severe damage in the hematopoietic system, which leads to bone marrow hyperplasia, pancytopenia, and aggravated tissue formation in bone marrow. Studies have shown that Toll-like receptor 4 (TLR4) has a protective effect against irradiation, but the underlying mechanism remains unclear. In this stu...

journal_title：Biochemical and biophysical research communications

authors： Sun Z,Wu K,Lin Q,Fei H,Jiang H,Chen T,Yuan Y

更新日期：2019-12-03 00:00:00

abstract：:The corticotropin releasing factor (CRF) plays a central role in regulating the activities of hypothalamic-pituitary-adrenal (HPA) axis in the presence of a variety of stressful stimuli via binding to its type 1 receptors (CRFR1). Despite that many peptidic or non-peptidic antagonists of CRFR1 have been developed to s...

journal_title：Biochemical and biophysical research communications

authors： Yu J,Zhuo R,Peng P,Liu X,Yan H,Zhang S,Zheng J,Wei X,Ma X

更新日期：2015-07-31 00:00:00