Abstract:
:Calcitonin gene-related peptide (CGRP) in porcine ventricular muscles. positive inotropic effects in the isolated, electrically driven false tendon of the porcine heart. Specific CGRP-binding sites were present in solubilized membrane fractions; the dissociation constant (Kd) and the maximum binding (Bmax) were 50.4 pM and 180 fmol/mg protein, respectively. SDS-PAGE analysis of CGRP-binding sites revealed the molecular mass of 70 K and 120 K. Few CGRP-like immunoreactive nerves were present in the ventricular muscle layer. These results indicate that CGRP activates specific receptor sites on the ventricular muscles and causes positive inotropic responses. CGRP receptors in ventricles are likely to be activated by circulating CGRP.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Miyauchi T,Sano Y,Hiroshima O,Yuzuriha T,Sugishita Y,Ishikawa T,Saito A,Goto Kdoi
10.1016/s0006-291x(88)81082-9subject
Has Abstractpub_date
1988-08-30 00:00:00pages
289-94issue
1eissn
0006-291Xissn
1090-2104pii
S0006-291X(88)81082-9journal_volume
155pub_type
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