当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > UVA-induced photoaging inhibits autophagic degradation by impairing lysosomal function in dermal fibroblasts.

UVA-induced photoaging inhibits autophagic degradation by impairing lysosomal function in dermal fibroblasts.

Abstract：

:Autophagy has been associated with a variety of diseases especially aging. Human dermal fibroblasts (HDFs) can internalize and then degrade elastin, collagen and advanced glycation end products (AGEs) in lysosomes, which plays prominent roles in extracellular matrix homeostasis and AGEs removal in the dermis. Although autophagy has been reported to be decreased in photoaged fibroblasts, the underlying mechanism and its relevance to photoaging remain elusive. Here, we showed that GFP-LC3 puncta per cell, LC3Ⅰ/Ⅱ conversion and p62 expression were significantly increased, whereas beclin1 expression was not altered in UVA-induced photoaged fibroblasts compared with non-photoaged control. Moreover, autophagic flux was not significantly affected by chloroquine treatment, but was remarkably induced by rapamycin treatment in photoaged fibroblasts, suggesting that UVA-induced photoaging might inhibit autophagy at the degradation stage. Further lysosomal function studies demonstrated that degradation of formed autophagosomes, LC3Ⅱprotein and DQ-Green BSA was all dramatically decreased in photoaged fibroblasts. LysoSensor yellow/blue DND 160 staining and flow cytometry assays demonstrated that photoaging obviously attenuated lysosomal acidification. Also, decreased expression of cathepsin B, L and D was found in photoaged fibroblasts. These data suggest that lowered lysosomal acidity and decreased cathepsins expression might contribute to the inhibition of autophagic degradation, which might be crucial in the development of photoaging through impairing intracellular degradation.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Huang Y,Li Y,Qu Y,Zheng Y,Ouyang M,Zhang Y,Lai W,Xu Q

doi

10.1016/j.bbrc.2019.08.103

subject

Has Abstract

pub_date

2019-10-22 00:00:00

pages

611-618

issue

4

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(19)31626-2

journal_volume

518

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Nonresponsiveness of immature B lymphocytes to anti-immunoglobulin is reversed by pronase.

    abstract：:Splenic B cells are induced to proliferate upon culture with antibody having specificity for surface membrane immunoglobulins. Cells treated with pronase, washed and then cultured with antibody, exhibited a greater than 5-fold enhancement of DNA synthesis whereas pronase treatment, per se, was not mitogenic. The prona...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(84)91609-7

    authors： Gollapudi SV,Ramanadham M,Kern M

    更新日期：1984-02-29 00:00:00

  • Isocitrate dehydrogenase 2 deficiency induces endothelial inflammation via p66sh-mediated mitochondrial oxidative stress.

    abstract：:Isocitrate dehydrogenase 2 (IDH2) is an essential enzyme in the mitochondrial antioxidant system, which produces nicotinamide adenine dinucleotide phosphate, and thereby defends against oxidative stress. We have shown that IDH2 downregulation results in mitochondrial dysfunction and reactive oxygen species (ROS) gener...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.07.117

    authors： Choi SJ,Piao S,Nagar H,Jung SB,Kim S,Lee I,Kim SM,Song HJ,Shin N,Kim DW,Irani K,Jeon BH,Park JW,Kim CS

    更新日期：2018-09-10 00:00:00

  • Phorbol ester induces differential membrane-association of protein kinase C subspecies in human platelets.

    abstract：:Human platelets contained proteins which cross-reacted with antisera specific for brain protein kinase C-alpha and -beta. When platelets were incubated with 12-O-tetradecanoylphorbol-13-acetate there was a rapid accumulation of protein kinase C-alpha in the particulate fraction associated with a loss of this subspecie...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)92635-1

    authors： Fournier A,Hardy SJ,Clark KJ,Murray AW

    更新日期：1989-06-15 00:00:00

  • Up-regulation of endothelial nitric oxide synthase through beta(2)-adrenergic receptor--the role of a beta-blocker with NO-releasing action.

    abstract：:We determined the existence and role of beta(2)-adrenergic receptor in cultured BAECs through the effect of a beta-blocker having NO releasing action; 3,4-dihydro-8(2-hydroxy-3-isopropylamino)-propoxy-3-nitroxy-2H-1-benzopyran; nipradilol on eNOS and eNOS regulatory protein caveolin-1. beta(2) receptor exists in BAECs...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.4177

    authors： Jayachandran M,Hayashi T,Sumi D,Thakur NK,Kano H,Ignarro LJ,Iguchi A

    更新日期：2001-01-26 00:00:00

  • Heterooligomers of the muscarinic receptor and G proteins purified from porcine atria.

    abstract：:Muscarinic receptor extracted from porcine atria in digitonin-cholate copurified with Galpha(o), Galpha(i1-3), and caveolins. The presence of complexes was confirmed by coimmunoprecipitation of the receptor, alpha-subunits, and caveolins in various combinations. Homooligomers of alpha(i2) were detected on Western blot...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.06.105

    authors： Ma AW,Pawagi AB,Wells JW

    更新日期：2008-09-12 00:00:00

  • Inhibition of B7-H3 reverses oxaliplatin resistance in human colorectal cancer cells.

    abstract：:B7-H3, an immunoregulatory protein, has been found highly expressed in several cancer types, and involved in cancer cell migration and invasion. Here, we investigated the role of B7-H3 in oxaliplatin resistance in colorectal cancer (CRC) cells. Transient silencing of B7-H3 enhanced oxaliplatin sensitivity by increasin...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.07.001

    authors： Zhang P,Chen Z,Ning K,Jin J,Han X

    更新日期：2017-08-26 00:00:00

  • Structural studies of the SET domain from RIZ1 tumor suppressor.

    abstract：:RIZ1 is a transcriptional regulator and tumor suppressor that catalyzes methylation of lysine 9 of histone H3. It contains a distinct SET domain, sometimes referred to as PR (PRDI-BF1 and RIZ1 homology) domain, that is responsible for its catalytic activity. We determined the solution structure of the PR domain from R...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.12.034

    authors： Briknarová K,Zhou X,Satterthwait A,Hoyt DW,Ely KR,Huang S

    更新日期：2008-02-15 00:00:00

  • Follistatin-like 1 attenuates differentiation and survival of erythroid cells through Smad2/3 signaling.

    abstract：:Hematopoiesis is a complex process tightly controlled by sets of transcription factors in a context-dependent and stage-specific manner. Smad2/3 transcription factor plays a central role in differentiation and survival of erythroid cells. Here we report that follistatin-like 1 (FSTL1) treatment impairs hemin-induced e...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.09.044

    authors： Wu J,Dong Y,Teng X,Cheng M,Shen Z,Chen W

    更新日期：2015-10-30 00:00:00

  • Specificity of thyroglobulin interactions with thyroid cells and membranes.

    abstract：:Homologous species specificity is demonstrated with bovine and human thyroglobulin in which the two terminal sugars of the B carbohydrate chain, sialic acid and galactose have been removed by enzymatic hydrolysis. The species specificity is demonstrated by measuring the ability of the deglycosylated thyroglobulin deri...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(83)90654-x

    authors： Rotella CM,Tanini A,Consiglio E,Shifrin S,De Luca M,Toccafondi R,Kohn LD

    更新日期：1983-08-12 00:00:00

  • Purification and characterization of 2-keto-3-deoxy-6-phosphogluconate aldolase from Azotobacter vinelandii: evidence that the enzyme is bifunctional towards 2-keto-4-hydroxy glutarate cleavage.

    abstract：:2-keto-3-deoxy-6-phosphogluconate aldolase (E.C. 4.1.2.14) has been purified in two chromatographic steps to 99% purity in 73% overall yield from Azotobacter vinelandii. The pure enzyme is a 70 kD trimeric Class I aldolase, inhibitable by bromopyruvate or pyruvate plus sodium borohydride, with a specific activity of 6...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.1471

    authors： Taha TS,Deits TL

    更新日期：1994-04-15 00:00:00

  • Degrading activity for human parathyroid hormone [PTH-(1-84)] in rat osteoblast-like osteosarcoma cell line UMR106.

    abstract：:The degrading activity for human parathyroid hormone [hPTH-(1-84)] was studied in a rat osteoblast-like osteosarcoma cell line UMR106. At 37 C,UMR106 cells degraded hPTH-(1-84) into fragments in a time-dependent manner, which was shown by a radioimmunoassay with the use of antibody recognizing the C-terminal and middl...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(86)80238-8

    authors： Yamaguchi T,Baba H,Fukase M,Kinoshita Y,Fujimi T,Fujita T

    更新日期：1986-12-15 00:00:00

  • Molecular cloning and DNA sequence analysis of the human guanine nucleotide-binding protein Go alpha.

    abstract：:The nucleotide sequence of human Go alpha was determined from a partial human brain cDNA clone and the sequence of the first two 5' coding exons of a human genomic Go alpha clone. Comparison of this sequence with bovine and rat Go alpha shows greater than 90% homology at the nucleotide and deduced amino acid level. Th...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(88)90463-9

    authors： Lavu S,Clark J,Swarup R,Matsushima K,Paturu K,Moss J,Kung HF

    更新日期：1988-01-29 00:00:00

  • PIH1D1, a subunit of R2TP complex, inhibits doxorubicin-induced apoptosis.

    abstract：:We have previously reported that the two components of R2TP complex, RNA polymerase II-associated protein 3 (RPAP3), and Reptin, regulate apoptosis. Here we characterize another component of the complex, PIH1 domain containing protein 1 (PIH1D1). PIH1D1 interacts with both RPAP3 and Monad in HEK293 or U2OS cells. PIH1...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.11.031

    authors： Inoue M,Saeki M,Egusa H,Niwa H,Kamisaki Y

    更新日期：2010-12-17 00:00:00

  • Cleavage of carcinoembryonic antigen induces metastatic potential in colorectal carcinoma.

    abstract：:Carcinoembryonic antigen (CEA), a widely used tumor marker, is attached by a glycosylphosphatidylinositol (GPI) anchor motif to the cell membrane. Recent study suggested that membrane-bound CEA might be cleaved by glycosylphosphatidylinositol-phospholipase D (GPI-PLD). We studied the effect of GPI-PLD on the cleavage ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.05.084

    authors： Yamamoto Y,Hirakawa E,Mori S,Hamada Y,Kawaguchi N,Matsuura N

    更新日期：2005-07-22 00:00:00

  • Antiviral potency of adenosine analogues: correlation with inhibition of S-adenosylhomocysteine hydrolase.

    abstract：:For a series of adenosine analogues a close correlation (r = 0.986) was found between their antiviral potency (against vesicular stomatitis virus) and their inhibitory effects (Ki/Km) on S-adenosylhomocysteine (AdoHcy) hydrolase; thus, in order of increasing inhibitory potency for both virus replication and AdoHcy hyd...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(85)91438-x

    authors： De Clercq E,Cools M

    更新日期：1985-05-31 00:00:00

  • IFN-gamma acts as anti-angiogenic cytokine in the human cornea by regulating the expression of VEGF-A and sVEGF-R1.

    abstract：:Inflammatory processes within the cornea are known to be associated with corneal neovascularization (CN). We examined the effects of inflammatory mediators on the expression of angiogenic factors by corneal cells. TNF-alpha and IL-1 induced VEGF-A secretion by corneal fibroblasts (HCRF) and this was inhibited signific...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.07.042

    authors： Kommineni VK,Nagineni CN,William A,Detrick B,Hooks JJ

    更新日期：2008-09-26 00:00:00

  • Biological properties of purified recombinant HCV particles with an epitope-tagged envelope.

    abstract：:To establish a simple system for purification of recombinant infectious hepatitis C virus (HCV) particles, we designed a chimeric J6/JFH-1 virus with a FLAG (FL)-epitope-tagged sequence at the N-terminal region of the E2 hypervariable region-1 (HVR1) gene (J6/JFH-1/1FL). We found that introduction of an adaptive mutat...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.04.081

    authors： Takahashi H,Akazawa D,Kato T,Date T,Shirakura M,Nakamura N,Mochizuki H,Tanaka-Kaneko K,Sata T,Tanaka Y,Mizokami M,Suzuki T,Wakita T

    更新日期：2010-05-14 00:00:00

  • A cysteine-free firefly luciferase retains luminescence activity.

    abstract：:A mutant of Photinus pyralis luciferase in which all four native cysteine residues are converted to serines retains about 10% of wild-type activity. This mutant should prove useful as a starting point for the introduction of biophysical probes of conformational changes associated with enzyme function. The activities o...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1950

    authors： Kumita JR,Jain L,Safroneeva E,Woolley GA

    更新日期：2000-01-07 00:00:00

  • AtSRP1, SMALL RUBBER PARTICLE PROTEIN HOMOLOG, functions in pollen growth and development in Arabidopsis.

    abstract：:To identify novel roles of SMALL RUBBER PARTICLE PROTEIN Homolog in the non-rubber-producing plant Arabidopsis (AtSRP1), we isolated a T-DNA-insertion knock-out mutant (FLAG_543A05) and investigated its functional characteristics. AtSRP1 is predominantly expressed in reproductive organs and is localized to lipid dropl...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.05.083

    authors： Chi YH,Kim SY,Lee ES,Jung YJ,Park JH,Paeng SK,Oh HT,Melencion SM,Alinapon CV,Lee SY

    更新日期：2016-06-24 00:00:00

  • Sulfiredoxin-1 alleviates high glucose-induced podocyte injury though promoting Nrf2/ARE signaling via inactivation of GSK-3β.

    abstract：:Hyperglycemia-induced podocyte injury plays a vital role in the development of diabetic nephropathy. Sulfiredoxin-1 (Srxn1) is emerging as a cytoprotective protein that protects from various insults in a wide range of cell types. However, whether Srxn1 is involved in regulating hyperglycemia-induced podocyte injury an...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.06.157

    authors： Shen Y,Chen S,Zhao Y

    更新日期：2019-09-03 00:00:00

  • Competitive peptide antagonists of ANF-induced cyclic guanosine monophosphate production.

    abstract：:Atrial natriuretic factor (isoleucine ANF 101-126), cleaved ANF (isoleucine ANF 101-105/106-126) and des (Gln 18, Ser 19, Gly 20, Leu 21, Gly 22) ANF 4-23-NH2 (C-ANF 4-23) stimulated cyclic guanosine monophosphate production (cGMP) by rat aortic vascular smooth muscle cells (VSMC) in culture. Cleaved ANF and ANF C4-23...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)91689-6

    authors： Abell TJ,Richards AM,Yandle TG,Espiner EA,Frampton C,Ikram H

    更新日期：1989-10-16 00:00:00

  • Effect of benzo[a]pyrene diol epoxide on expression of retinoic acid receptor-beta in immortalized esophageal epithelial cells and esophageal cancer cells.

    abstract：:Expression of retinoic acid receptor-beta (RAR-beta) is frequently lost in tobacco-related cancers. Benzo[a]pyrene diol epoxide (BPDE) is an active metabolite of tobacco procarcinogen benzo[a]pyrene and plays an important role in tobacco carcinogenesis. We therefore exposed SV-40 immortalized esophageal epithelial cel...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.4433

    authors： Song S,Xu XC

    更新日期：2001-03-09 00:00:00

  • Lysophosphatidylserine stimulates chemotactic migration in U87 human glioma cells.

    abstract：:Lysophosphatidylserine (LPS) was found to stimulate intracellular calcium increase in U87 human glioma cells. LPS also stimulated chemotactic migration of U87 human glioma cells, which was completely inhibited by pertussis toxin (PTX). Moreover, LPS was also found to stimulate ERK, p38 MAPK, JNK, and Akt activities in...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.06.117

    authors： Lee SY,Lee HY,Kim SD,Jo SH,Shim JW,Lee HJ,Yun J,Bae YS

    更新日期：2008-09-12 00:00:00

  • Establishment of a novel method for enriching osteoblast progenitors from adipose tissues using a difference in cell adhesive properties.

    abstract：:In the clinical field, cell-based therapies are used to treat bone defects. Adipose tissues contain many osteoblast progenitors, among other cell types. We separated mouse adipose tissue-derived stromal cells (ATSCs) according to their cell adhesive properties. Cells in a fraction adherent to the culture dishes 0.5h a...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.03.061

    authors： Hattori H,Ishihara M,Fukuda T,Suda T,Katagiri T

    更新日期：2006-05-19 00:00:00

  • Suppression of Helicobacter pylori-induced gastritis by green tea extract in Mongolian gerbils.

    abstract：:Since urease of Helicobacter pylori is essential for its colonization, we focused attention on foodstuffs which inhibit the activity of this enzyme. Among plant-derived 77 foodstuff samples tested, some tea and rosemary extracts were found to clearly inhibit H. pylori urease in vitro. In particular, green tea extract ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2003.09.066

    authors： Matsubara S,Shibata H,Ishikawa F,Yokokura T,Takahashi M,Sugimura T,Wakabayashi K

    更新日期：2003-10-24 00:00:00

  • Degradation of gamma D- and gamma s-crystallins in human lenses.

    abstract：:The aim of this study was to determine age-related degradation of gammaD-crystallin and the cleavage sites in the connecting peptide regions of the two domains of gammaD- and gammas-crystallins. The water-soluble (WS) proteins from lenses of donors of different ages and a purified gamma-crystallin fraction were analyz...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.9728

    authors： Srivastava OP,Srivastava K

    更新日期：1998-12-18 00:00:00

  • Nitric oxide mediates interleukin-1-induced prostaglandin E2 production by vascular smooth muscle cells.

    abstract：:We examined the possible participation of nitric oxide (NO) in the activation of cyclooxygenase, a heme-containing enzyme, induced by interleukin-1 (IL-1) in vascular smooth muscle cells (VSMC). IL-1 induced a delayed and prolonged release of both NO and prostaglandin E2 (PGE2) from VSMC. NG-monomethyl-L-arginine (L-N...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.1836

    authors： Inoue T,Fukuo K,Morimoto S,Koh E,Ogihara T

    更新日期：1993-07-15 00:00:00

  • Intra blood-cerebrospinal fluid-barrier detection of hepatitis B virus.

    abstract：:Hepatitis B, a major public health concern worldwide, is caused by hepatitis type B virus, a hepdnavirus that infects only human and certain nonhuman primates, and replicates strictly in hepatocytes. By using the techniques of slot and Southern blot DNA hybridization, and electron microscopy, the presence of HBV was i...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(87)90550-x

    authors： Pao CC,Wu SY,Hung IJ,Ng KT,Liaw YF,Lo SJ

    更新日期：1987-07-31 00:00:00

  • Type 1 angiotensin II receptor-associated protein ARAP1 binds and recycles the receptor to the plasma membrane.

    abstract：:The carboxyl terminus of the type 1 angiotensin II receptor (AT(1)) plays an important role in receptor phosphorylation, desensitization, and internalization. The yeast two-hybrid system was employed to isolate proteins associated with the carboxyl terminal region of the AT(1A) receptor. In the present study, we repor...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2003.09.154

    authors： Guo DF,Chenier I,Tardif V,Orlov SN,Inagami T

    更新日期：2003-10-31 00:00:00

  • rDLG6: a novel homolog of Drosophila DLG expressed in rat brain.

    abstract：:Drosophila DLG (Discs Large Tumor Suppresser Protein) is a component of septate junctions, and disruption of its gene leads to over growth of imaginal discs. Homologs of Drosophila DLG recently isolated from mammalian tissue have been classified as members of the MAGUK (Membrane Associated GUanylate Kinase) superfamil...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1723

    authors： Inagaki H,Maeda S,Lin KH,Shimizu N,Saito T

    更新日期：1999-11-19 00:00:00