当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Degrading activity for human parathyroid hormone [PTH-(1-84)] in rat osteoblast-like osteosarcoma cell line UMR106.

Degrading activity for human parathyroid hormone [PTH-(1-84)] in rat osteoblast-like osteosarcoma cell line UMR106.

Abstract：

:The degrading activity for human parathyroid hormone [hPTH-(1-84)] was studied in a rat osteoblast-like osteosarcoma cell line UMR106. At 37 C,UMR106 cells degraded hPTH-(1-84) into fragments in a time-dependent manner, which was shown by a radioimmunoassay with the use of antibody recognizing the C-terminal and middle regions of PTH molecule, whereas the degradation was completely suppressed at 4 C and failed to occur in the absence of the cells. The Lineweaver-Burk plot of this degrading activity at 37 C showed a fairly good linearity and gave a Km value of 5.1 X 10(-7) M. Reverse-phase high-performance liquid chromatography (HPLC) analysis of immunoreactive PTH fragments in the medium disclosed two peaks aside from intact PTH, indicating a limited PTH-hydrolyzing activity of UMR106 cells cleaving the molecule between at least two separate positions. This study suggests the possible involvement of osteoblasts on the metabolism of intact PTH.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Yamaguchi T,Baba H,Fukase M,Kinoshita Y,Fujimi T,Fujita T

doi

10.1016/s0006-291x(86)80238-8

subject

Has Abstract

pub_date

1986-12-15 00:00:00

pages

762-8

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(86)80238-8

journal_volume

141

pub_type

杂志文章

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