Hormone-sensitive lipase is critical mediators of acute exercise-induced regulation of lipolysis in rat adipocytes.

abstract：:A complementary DNA clone encoding serum amyloid A protein has been isolated from an acute rabbit liver cDNA library. Complete nucleotide sequence analysis reveals that the cloned gene contains a 24 bases 5' untranslated region, 369 bases coding region and a 106 bases 3' untranslated region. Primer extension analysis ...

journal_title：Biochemical and biophysical research communications

authors： Ray BK,Ray A

更新日期：1991-07-15 00:00:00

abstract：:Having ascertained using JC-1 as a probe that, in distinction with the controls, during capacitation boar sperm maintains high mitochondrial membrane potential (ΔΨ), to gain some insight into the role of mitochondria in capacitation, we monitored ΔΨ generation due to externally added metabolites either in hypotonicall...

journal_title：Biochemical and biophysical research communications

authors： Paventi G,Lessard C,Bailey JL,Passarella S

更新日期：2015-07-03 00:00:00

abstract：:Caspase-8 is a key mediator in various biological processes such as apoptosis, necroptosis, inflammation, T/B cells activation, and cell motility. Caspase-8 is characterized by the N-terminal tandem death effector domains (DEDs) and the C-terminal catalytic protease domain. The DEDs mediate diverse functions of caspas...

journal_title：Biochemical and biophysical research communications

authors： Shen C,Yue H,Pei J,Guo X,Wang T,Quan JM

更新日期：2015-07-31 00:00:00

abstract：:The major active protein phosphatase present in a rabbit skeletal muscle extract is associated with the glycogen particle and migrates in sucrose density gradient centrifugation as a Mr = 70,000 protein and contains modulator activity. Addition of extra modulator protein causes a time- and concentration-dependent conv...

journal_title：Biochemical and biophysical research communications

authors： Vandenheede JR,Yang SD,Merlevede W

更新日期：1983-09-30 00:00:00

abstract：:A new and simple method for the detection of antigenic proteins blotted onto nitrocellulose was developed. After transfer of spinach stromal proteins and purified phosphoribulokinase immunolabeling was performed with phosphoribulokinase antiserum, followed by a) Protein A-labeled colloidal gold particles, and b) by ho...

journal_title：Biochemical and biophysical research communications

authors： Surek B,Latzko E

更新日期：1984-05-31 00:00:00

abstract：:Tyrosine-specific phosphorylated proteins found exclusively on the cell surface of human astrocytomas were previously identified with murine monoclonal antibodies, designated as GA-17, GB-4 and GC-3. The purpose of this study was to further characterize the antigens and investigate the relationship between them and c-...

journal_title：Biochemical and biophysical research communications

authors： Kondo S,Miyatake S,Matsumoto M,Nakatsu S,Iwasaki K,Oda Y,Kikuchi H,Namba Y

更新日期：1992-01-31 00:00:00

abstract：:We have already cloned the cDNA for the HPC-1 antigen, a neuron-specific protein antigen from the rat brain. Here we report the molecular cloning of the bovine HPC-1 antigen homologue, and much strong sequence conservation between rat and bovine. By searching the recent protein data base, it was found that the HPC-1 a...

journal_title：Biochemical and biophysical research communications

authors： Inoue A,Akagawa K

更新日期：1992-09-16 00:00:00

abstract：:The metalated forms of metallothionein are well studied (particularly Zn-MT, Cu-MT and Cd-MT), but almost nothing is known about the chemical and structural properties of apometallothioneins despite their importance in initial metalation and subsequent demetalation. Electrospray ionization mass spectrometry was used t...

journal_title：Biochemical and biophysical research communications

authors： Summers KL,Mahrok AK,Dryden MD,Stillman MJ

更新日期：2012-08-24 00:00:00

abstract：:When the F1-ATPase from the thermophilic bacterium, PS3, was inactivated by 90% with 7-chloro-4-nitro[14C]benzofurazan ([14C]Nbf-Cl) at pH 7.3 and then gel-filtered, 1.25 mols of [14C]Nbf-O-Tyr and less than 0.1 mol of Nbf-N-Lys were formed per mol of enzyme. After adjusting the pH of the gel-filtered, modified enzyme...

journal_title：Biochemical and biophysical research communications

authors： Andrews WW,Yoshida M,Hill FC,Allison WS

更新日期：1984-09-28 00:00:00

abstract：:Previous work has demonstrated that pre-treatment of platelets with phorbol esters that activate protein kinase C eg phorbol 12-myristate 13-acetate (PMA) results in an inhibition of inositol phospholipid breakdown and granule secretion induced by physiological agonists such as thrombin and collagen. In the present st...

journal_title：Biochemical and biophysical research communications

authors： Krishnamurthi S,Kakkar VV

更新日期：1988-11-15 00:00:00

abstract：:Autophagy plays a crucial role in a wide array of physiological processes. To uncover the complex regulatory networks and mechanisms underlying basal autophagy, we performed a quantitative proteomics analysis of autophagy-deficient mouse embryonic fibroblast cells (MEFs) using iTRAQ labeling coupled with on-line 2D LC...

journal_title：Biochemical and biophysical research communications

authors： Zhuo C,Ji Y,Chen Z,Kitazato K,Xiang Y,Zhong M,Wang Q,Pei Y,Ju H,Wang Y

更新日期：2013-08-02 00:00:00

abstract：:Reactive oxygen species (ROS) play a crucial role in various physiological and pathological processes mediated by β-adrenergic receptors (β-ARs) in cardiomyocytes. However, the sources and signaling pathways involved in ROS production induced by acute β-AR activation have not yet been fully defined. In primary neonata...

journal_title：Biochemical and biophysical research communications

authors： Zhang J,Xiao H,Shen J,Wang N,Zhang Y

更新日期：2017-08-05 00:00:00

abstract：:Modification of microtubule (MT) dynamics is important for diverse aspects of cellular function including differentiation, cargo trafficking, migration, and adhesion. MTs also play a crucial role in the progression of neuronal development. The MT deacetylase Sirtuin 2 (Sirt2) and histone deacetylase 6 (HDAC6) regulate...

journal_title：Biochemical and biophysical research communications

authors： Jeong SG,Cho GW

更新日期：2017-01-01 00:00:00

abstract：:The liver X receptor (LXR) functions as a receptor for oxysterols and plays a critical role in the regulation of glucose and lipid metabolism. We recently described a synthetic LXR inverse agonist that displayed efficacy in treatment of hepatic steatosis in a mouse model of non-alcoholic fatty liver disease (NAFLD). T...

journal_title：Biochemical and biophysical research communications

authors： Griffett K,Burris TP

更新日期：2016-10-21 00:00:00

abstract：:In this study we identified a potential pro-apoptotic caspase gene, Bombyx mori(B. mori)ICE-2 (BmICE-2) which encoded a polypeptide of 284 amino acid residues, including a (169)QACRG(173) sequence which surrounded the catalytic site and contained a p20 and a p10 domain. BmICE-2 expressed in Escherichia coli (E. coli) ...

journal_title：Biochemical and biophysical research communications

authors： Yi HS,Pan CX,Pan C,Song J,Hu YF,Wang L,Pan MH,Lu C

更新日期：2014-02-28 00:00:00

abstract：:In this study, we established an in vitro hypoxic system driven by a self-regulated chemical reaction that proved effective for cell culture. The hypoxic device was modified from a 1.5 L polypropylene preservation box normally employed for food storage. Pyrogallic acid, sodium hydroxide, and sodium carbonate were diss...

journal_title：Biochemical and biophysical research communications

authors： Han N,Wang Z,Wang G,Yu J,Chen C,Huang H,Xu R

更新日期：2018-06-07 00:00:00

abstract：:Incorporation of [14C]serine into phosphatidylserine by the base exchange reaction in Ehrlich ascites tumor cells is inhibited by 30% by glucose and by 60% by 2-deoxyglucose (10 mM each). The inhibition by thapsigargin (0.2 microM) amounts to 80%. This inhibition is interpreted as being due to depletion of calcium sto...

journal_title：Biochemical and biophysical research communications

authors： Rakowska M,Wojtczak L

更新日期：1995-02-06 00:00:00

abstract：:Follistatin, activin and inhibin proteins are produced by granulosa cells, but the mechanisms controlling their production remain unclear. Here, we examined how the protein kinase A (PKA) and protein kinase C (PKC) pathways act and interact to regulate production of these proteins. Granulosa cells from immature rats w...

journal_title：Biochemical and biophysical research communications

authors： Miyanaga K,Erickson GF,DePaolo LV,Ling N,Shimasaki S

更新日期：1993-07-15 00:00:00

abstract：:Using recombinant DNA technology, the NH2 and COOH terminal domains of the human Factor VIII molecule were co-expressed in baby hamster kidney 21 (BHK21) cells using the vaccinia virus system. Procoagulant activity was detectable in cell supernatants, thus suggesting that the central portion present in the FVIII prote...

journal_title：Biochemical and biophysical research communications

authors： Pavirani A,Meulien P,Harrer H,Dott K,Mischler F,Wiesel ML,Mazurier C,Cazenave JP,Lecocq JP

更新日期：1987-05-29 00:00:00

abstract：:The close association of inflammation, angiogenesis and cancer progression is now highlighted, and in this study we especially focused on a close association of inflammation and lymphangiogenesis. We found that proinflammatory cytokine, interleukin-1beta (IL-1beta), could induce lymphangiogenesis in mouse cornea throu...

journal_title：Biochemical and biophysical research communications

authors： Watari K,Nakao S,Fotovati A,Basaki Y,Hosoi F,Bereczky B,Higuchi R,Miyamoto T,Kuwano M,Ono M

更新日期：2008-12-19 00:00:00

abstract：:Blockading the negative-regulatory CTLA-4 receptor has emerged as a powerful strategy with clinical potential to enhance T-cell responses. Some experimental tumors, for example, are rejected when anti-CTLA-4 antibodies are administered in vivo. The concise target cells and downstream events, however, remain to be defi...

journal_title：Biochemical and biophysical research communications

authors： Cepero E,Hnatyszyn HJ,Kraus G,Lichtenheld MG

更新日期：1998-06-29 00:00:00

abstract：:We recently reported that oviduct epithelial cells in culture produce endothelin (ET) and postulated that ET could play an important role in the contraction of the oviduct. In the present study using an organ chamber myograph system, we evaluated the contractile effects of ET-1 on bovine oviduct ampullary-isthmus segm...

journal_title：Biochemical and biophysical research communications

authors： Rosselli M,Imthurn B,Macas E,Keller PJ,Dubey RK

更新日期：1994-05-30 00:00:00

abstract：:Enhancer of Zeste 2 (EZH2) is the key enzymatic factor in Polycomb Repressive Complex 2 (PRC2), a transcriptional repressor which contributes to oncogenesis. Recent research has revealed the key role of aberrant EZH2 hyper-activity in human gliomas. Here, we examined the role of the lesser-known PRC2-associated PHD Fi...

journal_title：Biochemical and biophysical research communications

authors： Lu J,Ji H,Tang H,Xu Z

更新日期：2018-09-10 00:00:00

abstract：:Three small serum proteins (SSP-1, -2, and -3), with molecular masses of 6.5-10kDa, were isolated from Habu (Trimeresurus flavoviridis) serum, and the amino acid sequences were determined by protein and cDNA analysis. Despite only limited sequence identity to any mammalian prostatic secretory protein of 94 amino acids...

journal_title：Biochemical and biophysical research communications

authors： Aoki N,Sakiyama A,Deshimaru M,Terada S

更新日期：2007-07-27 00:00:00

abstract：:One of the earliest events following stimulation of human platelets with thrombin is a rise in the cytosolic pH, pHi, mediated by Na+/H+ exchange, and an increase in the cytosolic free Ca2+ concentration, [Ca2+]i. In the present study we investigated whether an increase in pHi alone, induced by the Na+/H+ ionophore mo...

journal_title：Biochemical and biophysical research communications

authors： Siffert W,Akkerman JW

更新日期：1989-06-30 00:00:00

abstract：:Five commonly used cationic liposome formulations were tested for their ability to deliver DNA to established subcutaneous human tumor xenografts in SCID mice. Liposomes were complexed with a mammalian expression plasmid containing the bacterial beta-galactosidase gene and delivered to tumors by direct injection. The ...

journal_title：Biochemical and biophysical research communications

authors： Egilmez NK,Iwanuma Y,Bankert RB

更新日期：1996-04-05 00:00:00

abstract：:GroEL-like particles of Thermus aquaticus are homo-oligomeric complexes of two stacked seven member rings, sedimenting in the gradient at 20S. The apparent molecular mass of the native particles is 820,000 (+/- 30,000). The protein complex is composed with one polypeptide of M(r) 59,000. Immunoblotting results and N-t...

journal_title：Biochemical and biophysical research communications

authors： Mikulík K,Benada O

更新日期：1993-12-15 00:00:00

abstract：:It is well known that the expression of osteocalcin, the only recognized osteoblast-specific protein, is regulated by 1 alpha-25-dihydroxyvitamin D3 through a distinct vitamin D3 receptor and a cis-acting DNA response element. In addition, recent data obtained with plasmid constructs have shown a positive interaction ...

journal_title：Biochemical and biophysical research communications

authors： Oliva A,Della Ragione F,Fratta M,Marrone G,Palumbo R,Zappia V

更新日期：1993-03-31 00:00:00

abstract：:Gene expression is the typical biological end point of interest following transfection. However, transcription may not accurately assess DNA uptake, or the ability of transfected DNA to be acted on by other enzymatic pathways. We have compared DNA uptake to gene expression and the unrelated enzymatic process of DNA do...

journal_title：Biochemical and biophysical research communications

authors： Pastwa E,Lubner EM,Mezhevaya K,Neumann RD,Winters TA

更新日期：2003-06-27 00:00:00

abstract：:Proliferating bovine aortic endothelial cells in culture do not express the insulin-like growth factor 1 binding protein 3 gene, in contrast to the genes encoding binding proteins 2, 4, 5, and 6. The binding protein 3 gene is activated only at cell confluency with continual transcription thereafter, for at least an ei...

journal_title：Biochemical and biophysical research communications

authors： Delafontaine P,Ku L,Anwar A,Hayzer DJ

更新日期：1996-05-15 00:00:00