当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Identification of an essential lysine residue in the beta subunit of the F1-ATPase from the thermophilic bacterium, PS3, using 7-chloro-4-nitro[14C]benzofurazan.

Identification of an essential lysine residue in the beta subunit of the F1-ATPase from the thermophilic bacterium, PS3, using 7-chloro-4-nitro[14C]benzofurazan.

Abstract：

:When the F1-ATPase from the thermophilic bacterium, PS3, was inactivated by 90% with 7-chloro-4-nitro[14C]benzofurazan ([14C]Nbf-Cl) at pH 7.3 and then gel-filtered, 1.25 mols of [14C]Nbf-O-Tyr and less than 0.1 mol of Nbf-N-Lys were formed per mol of enzyme. After adjusting the pH of the gel-filtered, modified enzyme to 9.0 and incubating it for 14 hrs. at 23 degrees C to promote O----N migration, 0.68 mol of Nbf-N-Lys were formed per mol of enzyme while about 16% of the original activity reappeared. Isolation of the subunits after the O----N migration showed that 90% of the incorporated 14C was present in the beta subunit, which contained 0.21 mols of [14C]Nbf-N-Lys per mol. A tryptic peptide which contained the majority of the 14C incorporated into the beta subunit was isolated and subjected to automatic amino acid sequence analysis contained 38 residues. The amino acid sequence immediately around the lysine residue labeled with [14C]Nbf-, K*, was found to be: ...I-G-L-F-G-G-A-G-V-G-K*-T-V-L-I-G... .

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Andrews WW,Yoshida M,Hill FC,Allison WS

doi

10.1016/s0006-291x(84)80238-7

subject

Has Abstract

pub_date

1984-09-28 00:00:00

pages

1040-6

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(84)80238-7

journal_volume

123

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Molecular cloning and DNA sequence analysis of the human guanine nucleotide-binding protein Go alpha.

    abstract：:The nucleotide sequence of human Go alpha was determined from a partial human brain cDNA clone and the sequence of the first two 5' coding exons of a human genomic Go alpha clone. Comparison of this sequence with bovine and rat Go alpha shows greater than 90% homology at the nucleotide and deduced amino acid level. Th...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(88)90463-9

    authors： Lavu S,Clark J,Swarup R,Matsushima K,Paturu K,Moss J,Kung HF

    更新日期：1988-01-29 00:00:00

  • Deimination of arginine residues in nucleophosmin/B23 and histones in HL-60 granulocytes.

    abstract：:Peptidylarginine deiminases (PADs) convert arginine residues in proteins into citrulline residues Ca(2+)-dependently. PAD V was recently found in granulocyte-differentiated HL-60 cells. To find a target of PAD V, we incubated HL-60 granulocytes with the calcium ionophore A23187 and studied deiminated proteins by immun...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.6303

    authors： Hagiwara T,Nakashima K,Hirano H,Senshu T,Yamada M

    更新日期：2002-01-25 00:00:00

  • Mutational analysis of cysteine residues within the extracellular domains of the human vasoactive intestinal peptide (VIP) 1 receptor identifies seven mutants that are defective in VIP binding.

    abstract：:We have used site-directed mutagenesis to investigate the requirement of cysteine residues in the extracellular domains of the human VIP 1 receptor for binding VIP. Cys37, Cys50, Cys63, Cys72, Cys86, Cys105 and Cys122 (N-terminal extracellular domain), Cys208 and Cys215 (second extracellular loop) and Cys288 (third ex...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.1897

    authors： Gaudin P,Couvineau A,Maoret JJ,Rouyer-Fessard C,Laburthe M

    更新日期：1995-06-26 00:00:00

  • Localization and effect of ectopic expression of CPT1c in CNS feeding centers.

    abstract：:Hypothalamic neurons monitor peripheral energy status and produce signals to adjust food intake and energy expenditure to maintain homeostasis. However, the molecular mechanisms by which these signals are generated remain unclear. Fluctuations in the level of hypothalamic malonyl-CoA are known to serve as an intermedi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.05.161

    authors： Dai Y,Wolfgang MJ,Cha SH,Lane MD

    更新日期：2007-08-03 00:00:00

  • (-)-Epigallocatechin gallate inhibition of osteoclastic differentiation via NF-kappaB.

    abstract：:People who regularly drink tea have been found to have a higher bone mineral density (BMD) and to be at less risk of hip fractures than those who do not drink it. Green tea catechins such as (-)-epigallocatechin gallate (EGCG) have been reported to increase osteogenic functioning in mesenchymal stem cells. However, it...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.01.007

    authors： Lin RW,Chen CH,Wang YH,Ho ML,Hung SH,Chen IS,Wang GJ

    更新日期：2009-02-20 00:00:00

  • Oscillatory shear alters endothelial hydraulic conductivity and nitric oxide levels.

    abstract：:This study addresses the role of nitric oxide (NO) and downstream signaling pathways in mediating the influences of oscillatory shear stress on the hydraulic conductivity (L(p)) of bovine aortic endothelial cell (BAEC) monolayers. Exposure of BAEC monolayers to 20 dyne/cm2 steady shear stress for 3 h induced a 3.3-fol...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/S0006-291X(02)00410-2

    authors： Hillsley MV,Tarbell JM

    更新日期：2002-05-24 00:00:00

  • Alpha-L-fucosidase from bull seminal plasma: its purification and acrosome reaction promoting property.

    abstract：:Alpha-L-fucosidase was purified from the bull seminal plasma by chromatography on DEAE-disk, octyl sepharose hydrophobic column and HPLC. The enzyme appeared to be pure as judged by the polyacrylamide gel electrophoresis both under the nondenaturing and denaturing conditions. The pure enzyme promoted the acrosome reac...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(86)90333-5

    authors： Srivastava PN,Arbtan K,Takei GH,Huang TT,Yanagimachi R

    更新日期：1986-06-30 00:00:00

  • Affinity labeling of delta opioid receptors by an enkephalin-derivative alkylating agent, DSLET-Mal.

    abstract：:Opioid binding properties of Tyr-D-Ser-Gly-Phe-Leu-Thr-NH-NH-Gly-Mal (DSLET-Mal), a novel enkephalin-framed affinity label, was determined in rat brain membranes. In competition studies the ligand showed high affinity for the delta opioid sites, labelled by [(3)H][Ile(5,6)]deltorphin II (K(i) = 8 nM), whereas its bind...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1682

    authors： Szatmári I,Orosz G,Medzihradszky K,Borsodi A

    更新日期：1999-11-19 00:00:00

  • Human dynamin-like protein interacts with the glycogen synthase kinase 3beta.

    abstract：:Members of the dynamin superfamily are implicated in vesicle trafficking. Using human glycogen synthase kinase 3 beta (Gsk-3 beta) as bait in the yeast two-hybrid system, we identified a novel human dynamin-like protein IV (HdynIV). When the full-length cDNA of HdynIV was sequenced, it showed that HdynIV's carboxyl te...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.9253

    authors： Hong YR,Chen CH,Cheng DS,Howng SL,Chow CC

    更新日期：1998-08-28 00:00:00

  • Identification of initiating agents in myoglobin-induced lipid peroxidation.

    abstract：:Reaction of metmyoglobin with peroxides is known to involve the formation, possibly via a porphyrin radical-cation, of a ferryl (iron(IV)-oxo) species and a protein radical; there is a little information available as to which of these species initiates the damage which is observed on incubating membrane fractions with...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)91730-z

    authors： Newman ES,Rice-Evans CA,Davies MJ

    更新日期：1991-09-30 00:00:00

  • Virus particle detection by solid phase immunocapture and atomic force microscopy.

    abstract：:A novel application of atomic force microscopy (AFM) in the rapid, label-free detection and identification of viruses is described. Multiplexed, miniaturized antibody domains were constructed using "ink-jet" protein arraying technology. The solid-phase affinity substrate termed the "ViriChip" was used in the immunocap...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2003.10.022

    authors： Nettikadan SR,Johnson JC,Mosher C,Henderson E

    更新日期：2003-11-14 00:00:00

  • Engineered U7 snRNA mediates sustained splicing correction in erythroid cells from β-thalassemia/HbE patients.

    abstract：:Repair of a splicing defect of β-globin pre-mRNA harboring hemoglobin E (HbE) mutation was successfully accomplished in erythroid cells from patients with β-thalassemia/HbE disorder by a synthetic splice-switching oligonucleotide (SSO). However, its application is limited by short-term effectiveness and requirement of...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.03.102

    authors： Preedagasamzin S,Nualkaew T,Pongrujikorn T,Jinawath N,Kole R,Fucharoen S,Jearawiriyapaisarn N,Svasti S

    更新日期：2018-04-30 00:00:00

  • Influence of GTP on system specific chaperone - Twin arginine signal peptide interaction.

    abstract：:Many bacterial respiratory redox enzymes depend on the twin-arginine translocase (Tat) system for translocation and membrane insertion. Tat substrates contain an N-terminal twin-arginine (SRRxFLK) motif serving as the targeting signal towards the translocon. Many Tat substrates have a system specific chaperone - redox...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.08.079

    authors： Cherak SJ,Turner RJ

    更新日期：2015-10-02 00:00:00

  • Follitropin signal transduction: alternative splicing of the FSH receptor gene produces a dominant negative form of receptor which inhibits hormone action.

    abstract：:We have studied the functional properties of an alternately spliced form of sheep testicular FSH receptor cDNA that codes for a protein similar to a previously described active receptor but differs in the carboxy terminus in sequence and is also shorter by 25 residues. The receptor expressed in HEK 293 cells fails to ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.1419

    authors： Sairam MR,Jiang LG,Yarney TA,Khan H

    更新日期：1996-09-24 00:00:00

  • Astroglial Ca2+ signaling is generated by the coordination of IP3R and store-operated Ca2+ channels.

    abstract：:Astrocytes play key roles in the central nervous system and regulate local blood flow and synaptic transmission via intracellular calcium (Ca2+) signaling. Astrocytic Ca2+ signals are generated by multiple pathways: Ca2+ release from the endoplasmic reticulum (ER) via the inositol 1, 4, 5-trisphosphate receptor (IP3R)...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.03.096

    authors： Sakuragi S,Niwa F,Oda Y,Mikoshiba K,Bannai H

    更新日期：2017-05-13 00:00:00

  • Expression of the fetal-oncogenic fibroblast growth factor-8/17/18 subfamily in human hematopoietic tumors.

    abstract：:The fibroblast growth factors (FGFs) are involved in hematopoiesis and tumorigenesis. However, little is known about the contribution of the FGFs identified within the past 10 years to leukemogenesis. To elucidate whether these FGFs (FGF-8, -9, -10, -11, -12, -13, -14, -16, -17, -18, -19, -20, and -21) are expressed i...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.07.153

    authors： Nezu M,Tomonaga T,Sakai C,Ishii A,Itoga S,Nishimura M,Matsuo Y,Tagawa M,Nomura F

    更新日期：2005-09-30 00:00:00

  • Immortalization of human myogenic progenitor cell clone retaining multipotentiality.

    abstract：:Human myogenic cells have limited ability to proliferate in culture. Although forced expression of telomerase can immortalize some cell types, telomerase alone delays senescence of human primary cultured myogenic cells, but fails to immortalize them. In contrast, constitutive expression of both telomerase and the E7 g...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.08.006

    authors： Hashimoto N,Kiyono T,Wada MR,Shimizu S,Yasumoto S,Inagawa M

    更新日期：2006-10-06 00:00:00

  • Constitutive hypophosphorylation of extracellular signal-regulated kinases-1/2 and down-regulation of c-Jun in human gastric adenocarcinoma.

    abstract：:Hyperphosphorylation of extracellular signal-regulated protein kinases-1/2 (ERK1/2) is known to promote cancer cell proliferation. We therefore investigated the constitutive phosphorylation levels of ERK1/2 and the expression of its downstream targets c-Fos, c-Jun, and cyclooxygenase-2 (COX-2) in biopsied human gastri...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.06.025

    authors： Wu WK,Sung JJ,Yu L,Li ZJ,Chu KM,Cho CH

    更新日期：2008-08-22 00:00:00

  • Analysis of allogeneic and syngeneic rat heart transplants using 23Na magnetic resonance spectroscopy.

    abstract：:This study defines the total sodium-23 magnetic resonance spectroscopy (23Na MRS) signal from in vivo heterotopic rat heart transplants in the early post-transplant period and examines the utility of this noninvasive method for monitoring allograft rejection. Measurements were performed at 4.7 T. Syngeneic (n = 6) and...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.0902

    authors： Waldrop SM,Alexander DZ,Lowry R,Winn KJ,Pearson TC,Constantinidis I

    更新日期：1996-06-14 00:00:00

  • Murine leukemia virus particles activate Rac1 in HeLa cells.

    abstract：:A number of viruses, when they bind to cells, activate intracellular signals that facilitate post-binding steps of infection. To determine if retroviruses activate intracellular signaling, we transduced HeLa cells with amphotropic retroviruses produced by TelCeB6 cells and examined cell lysates for activated Rac1. We ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.05.027

    authors： Krishna D,Le Doux JM

    更新日期：2006-07-07 00:00:00

  • Transgenic overexpression of USP15 in the heart induces cardiac remodeling in mice.

    abstract：:We found a novel protein-protein interaction between ubiquitin-specific protease 15 (USP15) and skeletal muscle LIM protein 1 (SLIM1): USP15 and SLIM1 directly bound under cell-free conditions and co-immunoprecipitated from the lysates of the cells, where they were co-expressed; and USP15 deubiquitinated SLIM1, result...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.01.012

    authors： Isumi Y,Hirata T,Saitoh H,Miyakawa T,Murakami K,Kudoh G,Doi H,Ishibashi K,Nakajima H

    更新日期：2011-02-11 00:00:00

  • Structural requirements for O2 sensing by the human tandem-P domain channel, hTREK1.

    abstract：:TREK1 is a member of the tandem-P domain K+ channel family which is expressed almost exclusively in the nervous system. It is modulated by a number of important factors including arachidonic acid and cell swelling. Since both factors are associated with brain ischemia, it has been suggested that activation of TREK1 ma...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.04.042

    authors： Miller P,Kemp PJ,Peers C

    更新日期：2005-06-17 00:00:00

  • Expression, purification and functional characterisation of a Kunitz-type module from chicken type VI collagen.

    abstract：:The primary amino acid sequence of the carboxyl-terminal portion of the alpha 3 chain of chicken type VI collagen (K-VI) presents a 58-residue motif with a high degree of homology with members of the Kunitz serine-proteinase inhibitors family. This module was cloned, expressed in E. coli, purified and compared to the ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.2570

    authors： Bearz A,Tolazzi G,Leonardi A,Pucillo C,Tell G,Colombatti A,Formisano S

    更新日期：1995-10-24 00:00:00

  • beta1 integrins modulate p66ShcA expression and EGF-induced MAP kinase activation in fetal lung cells.

    abstract：:ShcA proteins mediate Erk1/Erk2 activation by integrins and epidermal growth factor (EGF), and are expressed as p46ShcA, p52ShcA, and p66ShcA. Although p52ShcA and p46ShcA mediate Erk1/Erk2 activation, p66ShcA antagonizes Erk activation. p66ShcA is spatially regulated during lung development, leading us to hypothesize...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.02.058

    authors： Smith SM,Crowe DL,Lee MK

    更新日期：2006-04-14 00:00:00

  • Negatively charged residues (Asp378 and Asp379) in the last ten amino acids of the C-terminal tail of Cx43 hemichannels are essential for loop/tail interactions.

    abstract：:Connexin 43 (Cx43)-hemichannel activity is controlled by intramolecular interactions between cytoplasmic loop and C-terminal tail. We previously identified the last 10 amino acids of the C-terminal tail of Cx43 as essential for Cx43-hemichannel activity. We developed a cell-permeable peptide covering this sequence (TA...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.01.066

    authors： D'hondt C,Iyyathurai J,Wang N,Gourdie RG,Himpens B,Leybaert L,Bultynck G

    更新日期：2013-03-22 00:00:00

  • Low-level oxidative stress causes cell-cycle specific arrest in cultured cells.

    abstract：:Oxidative stress is involved in a multitude of pathological conditions. In the present study, cell cycle arrest was demonstrated in monolayer cultures of Chinese hamster ovary cells subjected to low-level oxidative stress induced by the addition of hydrogen peroxide. Fluorescence-activated cell sorting analysis charac...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.1645

    authors： Clopton DA,Saltman P

    更新日期：1995-05-05 00:00:00

  • Amino acid sequence analysis of human S100A7 (psoriasin) by tandem mass spectrometry.

    abstract：:The Ca(2+)-binding proteins regulate a number of cellular and extracellular activities and deregulations of S100 gene expression are associated with several human diseases. For example, S100A7 is upregulated in psoriatic skin, implicating a link with psoriasis, a chronic inflammatory dermatosis. We purified human S100...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.2772

    authors： Bürgisser DM,Siegenthaler G,Kuster T,Hellman U,Hunziker P,Birchler N,Heizmann CW

    更新日期：1995-12-05 00:00:00

  • Classification of ADAMTS binding sites: The first step toward selective ADAMTS7 inhibitors.

    abstract：:Genome-wide association studies identified ADAMTS7 as a risk locus for coronary artery disease. In carotid arteries of rats, neointima formation after balloon-mediated injury goes along with enhanced Adamts7 expression. Vice versa, Adamts7-deficient mice display reduced neointima formation following vascular injury. A...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.02.025

    authors： Müller M,Kessler T,Schunkert H,Erdmann J,Tennstedt S

    更新日期：2016-03-11 00:00:00

  • Sensitization of TNF-induced cytotoxicity in lung cancer cells by concurrent suppression of the NF-kappaB and Akt pathways.

    abstract：:Blockage of either nuclear factor-kappaB (NF-kappaB) or Akt sensitizes cancer cells to TNF-induced apoptosis. In this study, we investigated the undetermined effect of concurrent blockage of these two survival pathways on TNF-induced cytotoxicity in lung cancer cells. The results show that Akt contributes to TNF-induc...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.02.030

    authors： Wang X,Chen W,Lin Y

    更新日期：2007-04-13 00:00:00

  • Influence of divalent cations on the cytoskeletal dynamics of K562 cells determined by nano-scale bead tracking.

    abstract：:Cytoskeletal reorganization processes can be analyzed by studying the nanometer-scale spontaneous motion of beads bound to the cytoskeleton. The bead motion is determined by force fluctuations within the cytoskeletal network that originates from myosin motor activity and dynamic restructuring of cytoskeletal filaments...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.03.145

    authors： Alonso JL,Goldmann WH

    更新日期：2012-05-04 00:00:00