Purification and characterization of 2-keto-3-deoxy-6-phosphogluconate aldolase from Azotobacter vinelandii: evidence that the enzyme is bifunctional towards 2-keto-4-hydroxy glutarate cleavage.

abstract：:Using a cDNA glass microarray, the expression of 1081 genes in immature and mature dendritic cells (DCs) of two different individuals has been studied. The upregulation of mRNA transcripts of genes encoding the transcription factor ZFM1, Mos proto-oncogene serine/threonine-protein kinase, B-cell-specific transcription...

journal_title：Biochemical and biophysical research communications

authors： Lapteva N,Nieda M,Ando Y,Ide K,Hatta-Ohashi Y,Dymshits G,Ishikawa Y,Juji T,Tokunaga K

更新日期：2001-07-27 00:00:00

abstract：:ARF-related protein 1 (ARFRP1) is a membrane-associated GTPase which inhibits the ARF/Sec7-dependent activation of phospholipase D. We have recently shown that deletion of Arfrp1 in mice results in increased apoptosis of mesodermal cells during gastrulation, leading to early embryonic lethality. Here we describe the o...

journal_title：Biochemical and biophysical research communications

authors： Mueller AG,Joost HG,Schürmann A

更新日期：2002-03-22 00:00:00

abstract：:Most fish protamines contain two phosphorylatable sites both of which incorporate phosphate in vivo. Here we show that in two protamines (salmine A1 and clupeine Y1) the site more distant from the N-terminus (residues 20-21) is unaffected by cAMP-dependent protein kinase while it represents the main target for protein...

journal_title：Biochemical and biophysical research communications

authors： Ferrari S,Pinna LA

更新日期：1987-05-14 00:00:00

abstract：:Four clonal cell lines subcloned from a clonal population of fetal rat calvaria cells show a loss of type I collagen synthesis. Northern blot analysis showed that the level of alpha 1(I) collagen mRNA expression in each of the clonal populations parallels the level of collagen protein expression in each of these cell ...

journal_title：Biochemical and biophysical research communications

authors： Waye MM,Robinson R,Orfanides AG,Aubin JE

更新日期：1989-08-15 00:00:00

abstract：:We have identified a 35-amino acid antibiotic cecropin secreted by an established mosquito cell line. C7-10 cells from the vector mosquito, Aedes albopictus, were incubated with heat-killed Escherichia coli, and materials secreted into the cell culture supernatant were recovered by acid precipitation. Following batch ...

journal_title：Biochemical and biophysical research communications

authors： Sun D,Eccleston ED,Fallon AM

更新日期：1998-08-19 00:00:00

abstract：:The eukaryotic DNA cytosine-5-methyltransferase (E.C.2.1.1.37) is known to methylate cytosine in DNA mainly, but not exclusively in C-G. In the present study the minor, non-C-G recognition sequences of a rat DNA methyltransferase were analyzed by Maxam-Gilbert sequencing of in vitro methylated SV40 DNA. The enzyme met...

journal_title：Biochemical and biophysical research communications

authors： Hubrich-Kühner K,Buhk HJ,Wagner H,Kröger H,Simon D

更新日期：1989-05-15 00:00:00

abstract：:The organization of caveolae ultrastructures in the plasma membrane and the functions they dictate are mediated by membrane-embedded caveolins (caveolin-1, 2, 3) and peripherally attached cavins (cavin-1, 2, 3, 4). Mutations in caveolin and cavin genes are associated with a variety of human diseases. Cavin-1/PTRF muta...

journal_title：Biochemical and biophysical research communications

authors： Mohapatra A,Lokappa SB,Chaudhary N

更新日期：2020-01-15 00:00:00

abstract：:Hepcidin is a main regulator of iron metabolism, of which abnormal expression affects intestinal absorption and reticuloendothelial sequestration of iron by interacting with ferroportin. It is also noted that abnormal iron accumulation is one of the key factors to facilitate promotion and progression of cancer includi...

journal_title：Biochemical and biophysical research communications

authors： Toki Y,Sasaki K,Tanaka H,Yamamoto M,Hatayama M,Ito S,Ikuta K,Shindo M,Hasebe T,Nakajima S,Sawada K,Fujiya M,Torimoto Y,Ohtake T,Kohgo Y

更新日期：2016-08-05 00:00:00

abstract：:Based on two partial cDNA sequences, a full-length cDNA sequence for an actin-binding like protein previously named ABPL has been isolated and characterized. ABPL is homologous to the human actin-binding proteins ABP-280 and ABP-278. The predicted sequence for ABPL is 2,705 amino acids in length with a calculated mole...

journal_title：Biochemical and biophysical research communications

authors： Xie Z,Xu W,Davie EW,Chung DW

更新日期：1998-10-29 00:00:00

abstract：:The purpose of the present study is to characterize the ENaC-mediated Na+ absorption in human upper airway epithelia, nasal cavity, and paranasal sinus. To perform the purpose, we obtained epithelial cells from human nasal polyp (NP) and paranasal sinus mucosa (PSM) by endoscopic surgery. We measured the short-circuit...

journal_title：Biochemical and biophysical research communications

authors： Yasuda M,Niisato N,Miyazaki H,Iwasaki Y,Hama T,Dejima K,Hisa Y,Marunaka Y

更新日期：2007-10-26 00:00:00

abstract：:In normal development, the rate of cell differentiation is tightly controlled and critical for normal development and stem cell differentiation. However, the underlying mechanisms regulating the rate of the differentiation are unknown, and manipulation of the rate of the stem cell differentiation is currently difficul...

journal_title：Biochemical and biophysical research communications

authors： Minakawa T,Kanki Y,Nakamura K,Yamashita JK

更新日期：2020-03-26 00:00:00

abstract：:We recently developed the cellular model MVLN-15 in which estrogenic action can be detected by bioluminescence. Using this cellular model, we characterized the inhibitory effect of retinoic acid on the estrogen-dependent induction of luciferase transcription. We present evidence that i) the inhibitory effect of retino...

journal_title：Biochemical and biophysical research communications

authors： Demirpence E,Pons M,Balaguer P,Gagne D

更新日期：1992-02-28 00:00:00

abstract：:Mesenchymal stem cells (MSCs) have been isolated from various tissues and used for elucidating the pathogenesis of numerous diseases. In our previous in vitro study, we showed the existence of MSCs in human spinal ligaments and hypothesized that these MSCs contributed to the pathogenesis of ossification of spinal liga...

journal_title：Biochemical and biophysical research communications

authors： Chin S,Furukawa K,Ono A,Asari T,Harada Y,Wada K,Tanaka T,Inaba W,Mizukami H,Motomura S,Yagihashi S,Ishibashi Y

更新日期：2013-07-12 00:00:00

abstract：:Mitochondria dysfunction plays a significant role in the apoptosis of retinal cells. Diabetes activates retinal matrix metalloproteinases (MMP-9 and MMP-2), damages retinal mitochondria and activates the apoptotic machinery. This study is to investigate the temporal relationship between the activation of retinal MMPs ...

journal_title：Biochemical and biophysical research communications

authors： Santos JM,Tewari S,Lin JY,Kowluru RA

更新日期：2013-09-06 00:00:00

abstract：:Exposure of pancreatic islet B-cells to D-glucose and many other insulinotropic agents results in an increase of cytoplasmic calcium concentration, which triggers the exocytosis of secretroy granules. Previous studies have demonstrated that calcium itself, at concentrations ranging from 2 to 18 mM, is able to induce a...

journal_title：Biochemical and biophysical research communications

authors： Rasschaert J,Malaisse WJ

更新日期：1999-11-02 00:00:00

abstract：:Prostaglandin E(2) (PGE(2)) has been shown to negatively regulate adipogenesis. To explore to what extent PGE(2) inhibits the differentiation of cells to adipocytes and to examine whether its effect could be due to EP4 receptor signaling, we used microarrays to analyze the gene expression profiles of 3T3-L1 cells expo...

journal_title：Biochemical and biophysical research communications

authors： Sugimoto Y,Tsuboi H,Okuno Y,Tamba S,Tsuchiya S,Tsujimoto G,Ichikawa A

更新日期：2004-09-24 00:00:00

abstract：:Two new human genes encoding putative G protein-coupled receptors were cloned from genomic DNA following a degenerate PCR strategy. The predicted amino acid sequences of the proteins encoded by these genes have the characteristic motifs of chemokine receptors. This prompted us to name these genes CKR-L1 and CKR-L3. Ne...

journal_title：Biochemical and biophysical research communications

authors： Zaballos A,Varona R,Gutiérrez J,Lind P,Márquez G

更新日期：1996-10-23 00:00:00

abstract：:2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) and related compounds modulate various endocrine functions by enhancing ligand metabolism, altering hormone synthesis, down regulating receptor levels, and interfering with gene transcription. In the present study, we investigated the effects of TCDD on testosterone signal tr...

journal_title：Biochemical and biophysical research communications

authors： Jana NR,Sarkar S,Ishizuka M,Yonemoto J,Tohyama C,Sone H

更新日期：1999-03-24 00:00:00

abstract：:Long term function of human lung allografts is hindered by development of chronic rejection manifested as Bronchiolitis Obliterans Syndrome (BOS). We have previously identified the development of antibodies (Abs) following lung transplantation to K-alpha1-tubulin (KAT), an epithelial surface gap junction cytoskeletal ...

journal_title：Biochemical and biophysical research communications

authors： Tiriveedhi V,Angaswamy N,Weber J,Mohanakumar T

更新日期：2010-08-20 00:00:00

abstract：:The estrogen synthetase (aromatase, cytochrome P-450AROM) gene has been isolated from human genomic libraries and characterized. The restriction map of 43 positive clones obtained indicated that this enzyme is present as a single copy gene. The aromatase gene is unexpectedly large compared with other forms of the cyto...

journal_title：Biochemical and biophysical research communications

authors： Harada N,Yamada K,Saito K,Kibe N,Dohmae S,Takagi Y

更新日期：1990-01-15 00:00:00

abstract：:Spectroscopic analysis of the interaction of the epimeric 20-hydroperoxy derivatives of cholesterol with bovine adrenocortical cytochrome P-450scc preparations suggested the formation of a transient species. The intermediate was detected at 4 degrees C and characterized by a minimum at 412 nm in the difference spectru...

journal_title：Biochemical and biophysical research communications

authors： Larroque C,van Lier JE

更新日期：1983-04-29 00:00:00

abstract：:Several protein quality control systems in bacteria and/or mitochondrial matrix from lower eukaryotes are absent in higher eukaryotes. These are transfer-messenger RNA (tmRNA), The N-end rule ATP-dependent protease ClpAP, and two more ATP-dependent proteases, HslUV and ClpXP (in yeast). The lost proteases resemble the...

journal_title：Biochemical and biophysical research communications

authors： Lehmann G,Ziv T,Braten O,Admon A,Udasin RG,Ciechanover A

更新日期：2016-06-17 00:00:00

abstract：:To assess the relationship between cell proliferation and cell death, the mRNA accumulation of ornithine decarboxylase (ODC) and sulfated glycoprotein 2 (SGP-2) were measured in human peripheral blood lymphocytes (HPBL) 2-6 hours after stimulation with phytohemagglutinin (PHA). ODC is the rate limiting enzyme of polya...

journal_title：Biochemical and biophysical research communications

authors： Grassilli E,Bettuzzi S,Monti D,Ingletti MC,Franceschi C,Corti A

更新日期：1991-10-15 00:00:00

abstract：:We investigated the capacity of K-252a, an inhibitor of rat brain protein kinase C (PKC), to influence polymorphonuclear neutrophil (PMN) PKC and PMN activation with chemically and structurally dissimilar agonists. K-252a inhibited PMN PKC (IC50 = 0.58 microM), and caused a concentration-dependent (0.1-10 microM) inhi...

journal_title：Biochemical and biophysical research communications

authors： Smith RJ,Justen JM,Sam LM

更新日期：1988-05-16 00:00:00

abstract：:Nonsteroidal anti-inflammatory drugs (NSAIDs) have a preventive effect against colorectal cancer. Although inhibition of cyclooxygenase-2 plays a crucial role in the suppression of tumors, precise mechanisms of their action remain to be disclosed. To identify genes involved in the growth-suppressive effect of NSAIDs, ...

journal_title：Biochemical and biophysical research communications

authors： Iizaka M,Furukawa Y,Tsunoda T,Akashi H,Ogawa M,Nakamura Y

更新日期：2002-03-29 00:00:00

abstract：:Custom-designed zinc finger nucleases (ZFNs) are becoming powerful tools in gene targeting-the process of replacing a gene within a genome by homologous recombination. Here, we have studied the DNA cleavage by one such ZFN, DeltaQNK-FN, in order to gain insight into how ZFNs cleave DNA and how two inverted sites promo...

journal_title：Biochemical and biophysical research communications

authors： Mani M,Smith J,Kandavelou K,Berg JM,Chandrasegaran S

更新日期：2005-09-09 00:00:00

abstract：:We report a 39 kDa substrate for cholera and pertussis toxins is present in D. discoideum membranes. This protein did not co-migrate with alpha subunits of either Gs (45 kDa and 52 kDa) or Gi (41 kDa) from control mammalian cells. The presence of GTP or its non-hydrolyzable analogs enhanced the ADP-ribosylation in res...

journal_title：Biochemical and biophysical research communications

authors： Khachatrian L,Klein C,Howlett A

更新日期：1987-12-31 00:00:00

abstract：:The multidrug resistance proteins P-glycoprotein (Pgp) and MRP1 are drug-efflux pumps. In this study, we compared the nucleotide triphosphatase activities of the isolated N-terminal nucleotide binding domains (NBD1) of Pgp and MRP1, and explored the potential role of the phosphorylation target domain of Pgp on the reg...

journal_title：Biochemical and biophysical research communications

authors： Wilkes DM,Wang C,Aristimuño PC,Castro AF,Altenberg GA

更新日期：2002-08-16 00:00:00

abstract：:While chemotherapy is an important and widely used therapeutic for cancer, it may facilitate cancer metastasis. Herein, we report that human lung cancer cells exert higher invasion and metastasis after chemotherapy. In a human lung cancer xenograft model, chemotherapy promotes the cancer invasion and metastasis in HMG...

journal_title：Biochemical and biophysical research communications

authors： Wang K,Shan S,Wang S,Gu X,Zhou X,Ren T

更新日期：2018-06-07 00:00:00

abstract：:Carnitine deficiency associated with impairment of muscle function has been described in infants and young adults as a result of inherited or acquired disease but there are no reports on carnitine levels in healthy aging people. In this paper we show a marked reduction of carnitine and its derivatives in muscle, and o...

journal_title：Biochemical and biophysical research communications

authors： Costell M,O'Connor JE,Grisolía S

更新日期：1989-06-30 00:00:00