Autoregulation of bone sialoprotein gene in pre-osteoblastic and non-osteoblastic cells.

abstract：:PI3K activation is commonly observed in many human cancer cells. Survivin expression is elevated in cancer cells, and induced by some growth factors through PI3K activation. However, it is not clear whether PI3K activation is sufficient to induce survivin expression. To investigate the role of PI3K pathway in the regu...

journal_title：Biochemical and biophysical research communications

authors： Zhao P,Meng Q,Liu LZ,You YP,Liu N,Jiang BH

更新日期：2010-04-30 00:00:00

abstract：:The fibroblast growth factors (FGFs) play important roles in morphogenesis, angiogenesis, tissue remodeling, and carcinogenesis. Human FGF-20 has been cloned and characterized in this study. FGF-20 encodes a 211-amino-acid polypeptide with the FGF-core domain. A strong hydrophobic region was found in the FGF-core doma...

journal_title：Biochemical and biophysical research communications

authors： Kirikoshi H,Sagara N,Saitoh T,Tanaka K,Sekihara H,Shiokawa K,Katoh M

更新日期：2000-08-02 00:00:00

abstract：:We have previously described a novel putative 47kDa substrate for the protein kinase Akt (designated AS47) in 3T3-L1 adipocytes. In the present study, we have found by co-immunoprecipitation that AS47 was associated with cyclic nucleotide phosphodiesterase 3B (PDE3B) in lysates of 3T3-L1 adipocytes. The patterns of ex...

journal_title：Biochemical and biophysical research communications

authors： Chavez JA,Gridley S,Sano H,Lane WS,Lienhard GE

更新日期：2006-04-21 00:00:00

abstract：:Monodisperse bovine prothrombin was prepared and its molecular states under several conditions examined. The protein showed no tendency to self-associate in the absence of calcium. Calcium (4 mM) caused small increases in the apparent molecular weight of the protein which may or may not represent protein dimerization ...

journal_title：Biochemical and biophysical research communications

authors： Silversmith RE,Wei GJ,Nelsestuen GL

更新日期：1983-02-28 00:00:00

abstract：:Many plasma membrane proteins, including the epidermal growth factor (EGF) receptor, possess basic regions on the cytoplasmic surface of the membrane. To examine the function of these positively charged regions, we constructed mutated EGF receptor genes lacking this region by substitution of the basic amino acid resid...

journal_title：Biochemical and biophysical research communications

authors： Yamane K,Toyoshima C,Nishimura S

更新日期：1992-05-15 00:00:00

abstract：:In the present study we have examined the proteins involved in the insulin signaling cascade during and after differentiation of human adipocyte precursor cells and their correlation with glucose uptake. The differentiation of human adipocytes was characterized by a two- to threefold stimulation of glucose transport i...

journal_title：Biochemical and biophysical research communications

authors： Pederson T,Rondinone CM

更新日期：2000-09-16 00:00:00

abstract：:Lateral diffusion coefficients (D) of two surface differentiation antigens (sIgM and Bp35) were determined on interferon-sensitive (-IFs) or resistant (-IFr) Daudi cells by fluorescence photobleaching, using monospecific FITC-anti-IgM or PE-anti-Leu 16 probes. For untreated Daudi -IFs, mean (D) were 5.8 and 5.3 (x10(-...

journal_title：Biochemical and biophysical research communications

authors： Balint E,Aszalos A,Grimley PM

更新日期：1988-12-15 00:00:00

abstract：:We hypothesized that the mutational strand asymmetry is more strongly exerted upon the mitochondrial cytochrome b (Cytb) gene, which is distant from the origin of the light-strand replication (Ori(L)), than upon the ATPase subunit 6 (ATP6) gene, which is close to the Ori(L). To test this hypothesis, we determined the ...

journal_title：Biochemical and biophysical research communications

authors： Fuku N,Oshida Y,Takeyasu T,Guo LJ,Kurata M,Yamada Y,Sato Y,Tanaka M

更新日期：2002-02-01 00:00:00

abstract：:We have obtained two new mAbs to the carboxy-terminal region of fibronectin, namely P3D4 and P1F11, and have studied their binding sites and their ability to block lymphocyte adhesion to fibronectin. ELISA and Western blot analyses showed that P3D4 reacts with both fibronectin chains and both Hep II-containing fragmen...

journal_title：Biochemical and biophysical research communications

authors： Garcia-Pardo A,Sánchez-Aparicio P,Wayner EA

更新日期：1992-07-15 00:00:00

abstract：:Papiliocin is a 37-residue peptide isolated from the swallowtail butterfly Papilio xuthus. In this study, we found that Papiliocin induced the accumulation of reactive oxygen species (ROS) and hydroxyl radicals known to be important regulators of apoptosis in Candida albicans. To examine the relationship between the a...

journal_title：Biochemical and biophysical research communications

authors： Hwang B,Hwang JS,Lee J,Kim JK,Kim SR,Kim Y,Lee DG

更新日期：2011-04-29 00:00:00

abstract：:p73 is a member of the p53 family of transcription factors which plays an essential role in tumor suppression. p73 is associated with the sensitivity of cancer cells to chemotherapy and the prognosis of many cancers. In this study, we showed the ubiquitination-dependent degradation of p73 by the mitochondrial E3 ubiqu...

journal_title：Biochemical and biophysical research communications

authors： Min B,Ryu J,Chi SW,Yi GS

更新日期：2015-11-13 00:00:00

abstract：:We have developed a rapid and sensitive method for total DNA measurement using single-stranded DNA binding protein from E coli conjugated with horseradish peroxidase or urease. To detect DNA, the sample is heated or alkali treated to denature the DNA and then filtered through nylon or nitrocellulose membranes. After t...

journal_title：Biochemical and biophysical research communications

authors： Sheldon EL 3rd,Nagainis PA,Kung VT

更新日期：1989-11-30 00:00:00

abstract：:Calpain was generally believed to exist and function only in the cytoplasm. However, m-calpain has now been detected in the extracellular spaces of some kinds of tissue. In this study, we demonstrated the existence of m-calpain in the medium surrounding MC3T3-E1 cultures, and its activity by zymography. At the same ti...

journal_title：Biochemical and biophysical research communications

authors： Nishihara H,Nakagawa Y,Ishikawa H,Ohba M,Shimizu K,Nakamura T

更新日期：2001-07-20 00:00:00

abstract：:The genomes of several vertebrates contain two genes encoding proteins highly similar to threonine synthase (TS), even though the biosynthesis of l-threonine (l-Thr) is not known to occur in these animals. We report a bioinformatic analysis of the two TS-like genes, the recombinant expression of one murine TS homolog ...

journal_title：Biochemical and biophysical research communications

authors： Donini S,Percudani R,Credali A,Montanini B,Sartori A,Peracchi A

更新日期：2006-12-01 00:00:00

abstract：:The treatment of human platelets with the dibutyryl cyclic AMP (dbcAMP) revealed the presence of a 250 kDa protein which enhanced its GTP-binding activity. This protein was purified from platelet membranes by successive chromatographies on DEAE-cellulose, Ultrogel AcA34, Mono Q, HCA-hydroxyapatite, and TSK-3000SW colu...

journal_title：Biochemical and biophysical research communications

authors： Yada Y,Okano Y,Nozawa Y

更新日期：1990-10-15 00:00:00

abstract：:Multiple system atrophy is a neurodegenerative disease caused by abnormal α-synuclein (α-syn) accumulation in oligodendrocytes and neurons. We previously demonstrated that transgenic (Tg) mice that selectively overexpressed human α-syn in oligodendrocytes exhibited neuronal α-syn accumulation. Microtubule β-III tubuli...

journal_title：Biochemical and biophysical research communications

authors： Ito H,Nakayama K,Jin C,Suzuki Y,Yazawa I

更新日期：2012-11-23 00:00:00

abstract：:In this work we have investigated the transfer of radioactive palmitic acid between membrane phospholipids and acyl-L-carnitines in intact human erythrocytes. During the incubation period of labeled erythrocyte in non-defatted bovine serum albumin, radioactivity in phosphatidylcholine and phosphatidylethanolamine incr...

journal_title：Biochemical and biophysical research communications

authors： Arduini A,Tyurin V,Tyuruna Y,Arrigoni-Martelli E,Molajoni F,Dottori S,Federici G

更新日期：1992-08-31 00:00:00

abstract：:The role of heteromeric phosphoinositide (PI) 3-kinase activity in insulin signal transduction was studied by investigating the effects of (i) overexpression of a dominant negative mutant p85 (delta p85) that lacks the binding site for p110 (delta p85-overexpressing cells) and (ii) inhibition of PI 3-kinase activity b...

journal_title：Biochemical and biophysical research communications

authors： Hara K,Yonezawa K,Sakaue H,Kotani K,Kotani K,Kojima A,Waterfield MD,Kasuga M

更新日期：1995-03-17 00:00:00

abstract：:WRKY transcription factors are key regulators in regulating abiotic or biotic stress response in Arabidopsis. Previous studies showed that WRKY30 expression was induced by oxidative stress treatment, fungal elicitor, SA and ABA. However, functions of WRKY30 on viral defense are not well studied. Here, we found that Ar...

journal_title：Biochemical and biophysical research communications

authors： Zou L,Yang F,Ma Y,Wu Q,Yi K,Zhang D

更新日期：2019-09-10 00:00:00

abstract：:α-Isopropylmalate synthase (α-IPMS) is a multi-domain protein catalysing the condensation of α-ketoisovalerate (α-KIV) and acetyl coenzyme A (AcCoA) to form α-isopropylmalate. This reaction is the first committed step in the leucine biosynthetic pathway in bacteria and plants, and α-IPMS is allosterically regulated by...

journal_title：Biochemical and biophysical research communications

authors： Huisman FH,Squire CJ,Parker EJ

更新日期：2013-04-05 00:00:00

abstract：:The proB proA operons (which are blocked by the feedback inhibition of proline production) of Escherichia coli wild type or with the mutation proBosm, blocking feedback inhibition effect of proline production, were cloned in a broad host range shuttle vector pVA 12-2. The hybrid plasmids pLVA(proB+A+) and pNSA(proBosm...

journal_title：Biochemical and biophysical research communications

authors： Neumivakin LV,Solovjev VP,Sokhansanj A,Tilba VA,Moseiko NA,Shachbasian RV,Piruzian ES

更新日期：1995-12-26 00:00:00

abstract：:Myosin light chain kinase (MLCK) is a multifunctional regulatory protein of smooth muscle contraction [IUBMB Life 51 (2001) 337, for review]. The well-established mode for its regulation is to phosphorylate the 20 kDa myosin light chain (MLC 20) to activate myosin ATPase activity. MLCK exhibits myosin-binding activity...

journal_title：Biochemical and biophysical research communications

authors： Gao Y,Kawano K,Yoshiyama S,Kawamichi H,Wang X,Nakamura A,Kohama K

更新日期：2003-05-23 00:00:00

abstract：:Cancer cell molecular mimicry of stem cells (SC) follows with enhanced proliferative and renewal capacities. In support, numerous mediators of SC self-renewal have been evinced to exhibit oncogenic potential. More and more researches showed that the embryonic stem cell self-renewal genes express in various cancer cell...

journal_title：Biochemical and biophysical research communications

authors： Deng L,Xiang X,Yang F,Xiao D,Liu K,Chen Z,Zhang R,Feng G

更新日期：2017-08-19 00:00:00

abstract：:Porphyromonas gingivalis acquires heme through an outer-membrane heme transporter HmuR and heme-binding hemophore-like lipoprotein HmuY. Here, we compare binding of iron(III) mesoporphyrin IX (mesoheme) and iron(III) deuteroporphyrin IX (deuteroheme) to HmuY with that of iron(III) protoporphyrin IX (protoheme) and pro...

journal_title：Biochemical and biophysical research communications

authors： Wojaczyński J,Wójtowicz H,Bielecki M,Olczak M,Smalley JW,Latos-Grażyński L,Olczak T

更新日期：2011-07-29 00:00:00

abstract：:Ginseng, refering to the roots of the species of the genus Panax ginseng, has been widely used in traditional oriental medicine for its wide spectrum of medicinal effects, such as anti-inflammatory, anti-tumorigenic, adaptogenic, and anti-aging activities. Many of its medicinal effects are attributed to the triterpene...

journal_title：Biochemical and biophysical research communications

authors： Min JK,Kim JH,Cho YL,Maeng YS,Lee SJ,Pyun BJ,Kim YM,Park JH,Kwon YG

更新日期：2006-10-27 00:00:00

abstract：:Genetic predisposition is necessary for polycystic kidney disease (PKD) initiation, although there are other, incompletely identified downstream processes that are required for cyst growth. Their characterization may provide a unique opportunity for clinical interventions. One of the poorly studied phenomena in PKD is...

journal_title：Biochemical and biophysical research communications

authors： Arkhipov SN,Pavlov TS

更新日期：2019-05-21 00:00:00

abstract：:Molecular structure of the ligand binding domain of hAhR has been modelled by homology modelling techniques and used for docking simulations with dioxin and nine more xenobiotics and endogenous ligands. The study evidences that different sites may bind these ligands, whereas only one binding site has been previously i...

journal_title：Biochemical and biophysical research communications

authors： Salzano M,Marabotti A,Milanesi L,Facchiano A

更新日期：2011-09-23 00:00:00

abstract：:An organoid is a complex, multi-cell three-dimensional (3D) structure that contains tissue-specific cells. Epithelial stem cells, which are marked by leucine-rich repeat-containing G-protein coupled receptor 5 (Lgr5), have the potential for self-renewal and expansion as organoids. However, in the case of intestinal or...

journal_title：Biochemical and biophysical research communications

authors： Hahn S,Kim MS,Choi SY,Jeong S,Jee J,Kim HK,Jeong SY,Shin H,Kim HS,Park JS,Yoo J

更新日期：2019-01-08 00:00:00

abstract：:Fut8 (α1,6-Fucosyltransferase) heterozygous knock-out (Fut8(+/-)) mice had an increased influx of inflammatory cells into the lungs, and this was associated with an up-regulation of matrix metalloproteinases, MMP-2 and MMP-9, after treatment with porcine pancreatic elastase (PPE), exhibiting an emphysema-prone phenoty...

journal_title：Biochemical and biophysical research communications

authors： Kamio K,Yoshida T,Gao C,Ishii T,Ota F,Motegi T,Kobayashi S,Fujinawa R,Ohtsubo K,Kitazume S,Angata T,Azuma A,Gemma A,Nishimura M,Betsuyaku T,Kida K,Taniguchi N

更新日期：2012-07-20 00:00:00

abstract：:To determine whether insulin resistance increases expression of plasminogen activator inhibitor type-1 (PAI-1) in the heart, studies were performed in 22 mice with and 38 without myocardial infarction. Insulin resistance in transgenic animals genetically rendered insulin resistant was confirmed with the use of intrape...

journal_title：Biochemical and biophysical research communications

authors： Sobel BE,Schneider DJ,Lee YH,Pratley RE

更新日期：2006-07-21 00:00:00