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Isolation of plasma membrane fragments from cultured murine melanoma cells.

Abstract：

:The various manipulations involved in the isolation of membrane fragments from culture fluids of murine melanoma cells were examined to discern their effect on membrane fragment structure. Ultracentrifugation and gel chromatography were compared using the presence of marker enzymes and the sensitivity to a non-ionic detergent (Triton X-100). Fractionation of media by gel chromatography resulted in only one major form of membrane particles, while ultracentrifugation, followed by resuspension, produced at least two major populations from the identical material. These results indicate that the optimal procedure for membrane fragment isolation is fractionation by an agarose-containing gel, followed by concentration using PEG 20,000.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Taylor DD,Chou IN,Black PH

doi

10.1016/0006-291x(83)91749-7

subject

Has Abstract

pub_date

1983-06-15 00:00:00

pages

470-6

issue

2

eissn

0006-291X

issn

1090-2104

pii

0006-291X(83)91749-7

journal_volume

113

pub_type

杂志文章

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