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G-rich, a Drosophila selenoprotein, is a Golgi-resident type III membrane protein.

Abstract：

:G-rich is a Drosophila melanogaster selenoprotein, which is a homologue of human and mouse SelK. Subcellular localization analysis using GFP-tagged G-rich showed that G-rich was localized in the Golgi apparatus. The fusion protein was co-localized with the Golgi marker proteins but not with an endoplasmic reticulum (ER) marker protein in Drosophila SL2 cells. Bioinformatic analysis of G-rich suggests that this protein is either type II or type III transmembrane protein. To determine the type of transmembrane protein experimentally, GFP-G-rich in which GFP was tagged at the N-terminus of G-rich, or G-rich-GFP in which GFP was tagged at the C-terminus of G-rich, were expressed in SL2 cells. The tagged proteins were then digested with trypsin, and analyzed by Western blot analysis. The results showed that the C-terminus of the G-rich protein was exposed to the cytoplasm indicating it is a type III microsomal membrane protein. G-rich is the first selenoprotein identified in the Golgi apparatus.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Chen CL,Shim MS,Chung J,Yoo HS,Ha JM,Kim JY,Choi J,Zang SL,Hou X,Carlson BA,Hatfield DL,Lee BJ

doi

10.1016/j.bbrc.2006.07.203

subject

Has Abstract

pub_date

2006-10-06 00:00:00

pages

1296-301

issue

4

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(06)01742-6

journal_volume

348

pub_type

杂志文章

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