Effects of sequence selective drugs on the gel mobility of a bent DNA fragment.

abstract：:Insulin gene was induced into differentiated hepatic cell line. These cells released immunoreactive insulin (IRI) of which molecular weight was greater than mature insulin. The secretion of IRI was responsive to glucose concentration and inhibited by glucose metabolism antagonist, 2-deoxy glucose (2-DOG). The mRNA of ...

journal_title：Biochemical and biophysical research communications

authors： Hayashi M,Taguchi M,Fujita T

更新日期：1997-04-17 00:00:00

abstract：:Cystic fibrosis (CF) is caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene that encodes a small conductance cAMP-activated chloride ion channel. In the CF pancreatic duct, mutations in CFTR cause a reduction in bicarbonate secretion. This is thought to result from CFTR operating...

journal_title：Biochemical and biophysical research communications

authors： Hyde K,Harrison D,Hollingsworth MA,Harris A

更新日期：1999-09-24 00:00:00

abstract：:In this study, we assessed the profile of cell proliferation, Na,K-ATPase activity and apoptosis in primary cultures of proximal tubule cells. Freshly isolated proximal tubules exhibited apoptosis very early in culture: floated cells within 6 hr; attached tubule cells within 20 hr. The onset of apoptosis in either att...

journal_title：Biochemical and biophysical research communications

authors： Tang MJ,Cheng YR,Lin HH

更新日期：1996-01-26 00:00:00

abstract：:Neurogenin-3 (ngn-3) expression is critical for endocrine development in the developing pancreas. We found that when ngn-3 was inhibited in an E11.5 pancreas, using either morpholino antisense or siRNA, it led to a significant decrease in endocrine differentiation after seven days in culture. Endocrine differentiation...

journal_title：Biochemical and biophysical research communications

authors： Prasadan K,Tulachan S,Guo P,Shiota C,Shah S,Gittes G

更新日期：2010-06-11 00:00:00

abstract：:Human MCF7 breast tumor cells grew as estrogen-dependent tumors in nude mice. In contrast, they were not estrogen-dependent for proliferation in serumless culture media. Charcoal-dextran stripped female human serum supplemented media (5% to 40%) inhibited their proliferation in a dose dependent pattern. Estrogens reve...

journal_title：Biochemical and biophysical research communications

authors： Soto AM,Sonnenschein C

更新日期：1984-08-16 00:00:00

abstract：:MicroRNAs (miRNAs) are involved in controlling hepatocyte proliferation during liver regeneration. In this study, we established the miRNAs-expression patterns of primary hepatocytes in vitro under stimulation of epidermal growth factor (EGF), and found that microRNA-21 (miR-21) was appreciably up-regulated and peaked...

journal_title：Biochemical and biophysical research communications

authors： Yan-nan B,Zhao-yan Y,Li-xi L,jiang Y,Qing-jie X,Yong Z

更新日期：2014-01-17 00:00:00

abstract：:Natural Killer (NK) cells are highly mobile, specialized sub-populations of lymphocytic cells that survey their host to identify and eliminate infected or tumor cells. They are one of the key players in innate immunity and do not need prior activation through antigen recognition to deliver cytotoxic packages and relea...

journal_title：Biochemical and biophysical research communications

authors： Tompkins N,MacKenzie B,Ward C,Salgado D,Leidal A,McCormick C,Pohajdak B

更新日期：2014-08-22 00:00:00

abstract：:An endonuclease that specifically removes 8-hydroxyguanine (oh8Gua) from DNA has been isolated from Escherichia coli. As the amount of oh8Gua produced in DNA of X-ray-irradiated mice is known to decrease with time after irradiation, an attempt was made to find a similar activity in human polymorphonuclear neutrophils ...

journal_title：Biochemical and biophysical research communications

authors： Chung MH,Kim HS,Ohtsuka E,Kasai H,Yamamoto F,Nishimura S

更新日期：1991-08-15 00:00:00

abstract：:Tumor necrosis factor receptor-associated factor 2 (TRAF2) has been demonstrated that it plays a significant role in cell death receptor signal transduction. The purpose of this study was to investigate the expression of TRAF2 and its possible role in FJOA. We observed an up-regulation of TRAF2 in FJOA by immunohistoc...

journal_title：Biochemical and biophysical research communications

authors： Jiang J,Zhang J,Wu C,Guo X,Chen C,Bao G,Sun Y,Chen J,Xue P,Xu G,Cui Z

更新日期：2018-09-10 00:00:00

abstract：:While chemotherapy is an important and widely used therapeutic for cancer, it may facilitate cancer metastasis. Herein, we report that human lung cancer cells exert higher invasion and metastasis after chemotherapy. In a human lung cancer xenograft model, chemotherapy promotes the cancer invasion and metastasis in HMG...

journal_title：Biochemical and biophysical research communications

authors： Wang K,Shan S,Wang S,Gu X,Zhou X,Ren T

更新日期：2018-06-07 00:00:00

abstract：:Protein phosphatase 2C (PP2C) is one of the four major protein serine/threonine phosphatases of eukaryotes and is implicated in the regulation of various cellular functions. With the goal of elucidating the mechanism responsible for regulating PP2C functions, we investigated the significance of phosphorylation of fiss...

journal_title：Biochemical and biophysical research communications

authors： Kobayashi T,Sadaie M,Ohnishi M,Wang H,Ikeda S,Hanada M,Yanagawa Y,Nakajima T,Tamura S

更新日期：1998-10-09 00:00:00

abstract：:Various dehydrogenation polymers (the so-called synthetic lignins) were synthesized from four different phenylpropenoids by endwise and bulk polymerizations and investigated for their anti-influenza virus activity. All of these materials suppressed the plaque formation of influenza virus infecting MDCK cells and the R...

journal_title：Biochemical and biophysical research communications

authors： Sakagami H,Nagata K,Ishihama A,Oh-hara T,Kawazoe Y

更新日期：1990-11-15 00:00:00

abstract：:Different progestogens are widely used in hormonal therapy and mediate their therapeutic actions via the progesterone receptor (PR). Little published data exist on their relative efficacies and potencies via the PR, while those available may be confounded by off-target receptors, different methodologies and model syst...

journal_title：Biochemical and biophysical research communications

authors： Enfield K,Cartwright M,Toit RL,Avenant C,Africander D,Hapgood JP

更新日期：2020-12-17 00:00:00

abstract：:The yeast transcriptional regulator Hap1 promotes both transcriptional activation and repression. Previous studies have shown that Hap1 binds to the promoter of its own gene and represses its transcription. In this report, we identified the DNA site that allows Hap1-binding with high affinity. This Hap1-binding site c...

journal_title：Biochemical and biophysical research communications

authors： Xin X,Lan C,Lee HC,Zhang L

更新日期：2007-10-12 00:00:00

abstract：:Endothelin-1 (ET-1) is a 21-amino acid vasoactive peptide produced from a 39-amino acid biologically inactive peptide, big ET-1, by the action of endothelin-converting enzyme-1 (ECE-1). We investigated gastric mucosal expression of ECE-1 during a 16 h course of inflammatory responses associated with gastric mucosal in...

journal_title：Biochemical and biophysical research communications

authors： Slomiany BL,Slomiany A

更新日期：2000-03-16 00:00:00

abstract：:Insect development is controlled by the combined actions of ecdysteroid and juvenile hormones. Transcriptional control by ecdysteroid hormones is mediated via two nuclear receptor superfamily members, ecdysone receptor (EcR) and its heterodimeric partner, ultraspiracle (USP). Although the ecdysteroid hormone 20-hydrox...

journal_title：Biochemical and biophysical research communications

authors： Maki A,Sawatsubashi S,Ito S,Shirode Y,Suzuki E,Zhao Y,Yamagata K,Kouzmenko A,Takeyama K,Kato S

更新日期：2004-07-16 00:00:00

abstract：:The Rex-family repressors sense redox levels by alternative binding to NADH or NAD(+). RSP is the homologue of Rex in Thermoanaerobacter ethanolicus JW200(T) and regulates ethanol fermentation in this obligate anaerobe. The dimeric repressor binds to DNA by an open conformation. The crystal structure of RSP/α-NAD(+) c...

journal_title：Biochemical and biophysical research communications

authors： Zheng Y,Ko TP,Yang Y,Shao W,Guo RT

更新日期：2015-01-16 00:00:00

abstract：:Ceramide kinase (CERK) converts ceramide to ceramide-1-phosphate (C1P), which has recently emerged as a new bioactive molecule capable of regulating diverse cellular functions. The N-terminus of the CERK protein encompasses a sequence motif known as a pleckstrin homology (PH) domain. Although the PH domain was previou...

journal_title：Biochemical and biophysical research communications

authors： Kim TJ,Mitsutake S,Igarashi Y

更新日期：2006-04-07 00:00:00

abstract：:Stimulation of human T cell line Jurkat results in rapid tyrosine phosphorylation of a 35-38 kDa protein that is found in large and buoyant detergent-resistant membrane microdomains containing also glycosylphosphatidylinositol (GPI)-anchored proteins, glycolipids and Src-family protein tyrosine kinases ("GPI-microdoma...

journal_title：Biochemical and biophysical research communications

authors： Brdiĉka T,Cerný J,Horejŝí V

更新日期：1998-07-20 00:00:00

abstract：:Cysteinyl leukotrienes (cysLTs), which include leukotriene C4 (LTC4), are the predominant class of LTs synthesized by mast cells. CysLTs can induce many of the abnormalities seen in asthma. LTC4 is generated by the conjugation of LTA4 with reduced glutathione (GSH) by LTC4 synthase. During screening of the effects of ...

journal_title：Biochemical and biophysical research communications

authors： Seki K,Hisada T,Kawata T,Kamide Y,Dobashi K,Yamada M,Mori M,Okajima F,Ishizuka T

更新日期：2013-09-27 00:00:00

abstract：:Inspired by recent interest in torsion of the central pair microtubules in eukaryotic flagella, a novel thin-walled elastic beam model is suggested to study critical condition under which uniform bending of a flagellum will cause lateral/torsional buckling of the central pair. The model is directed to the central pair...

journal_title：Biochemical and biophysical research communications

authors： Li C,Ru CQ,Mioduchowski A

更新日期：2006-12-08 00:00:00

abstract：:The exon/intron organization and the structure of the 5' flanking region of the human thyrotropin receptor gene (hTSH-R) were determined. The hTSH-R gene spans more than 60 kb and is split into ten exons. The extracellular domain is encoded by the first nine exons and part of the last exon, whereas the transmembrane a...

journal_title：Biochemical and biophysical research communications

authors： Gross B,Misrahi M,Sar S,Milgrom E

更新日期：1991-06-14 00:00:00

abstract：:Microsomal cytochrome P450 reductase catalyzes the one-electron transfer from NADPH via FAD and FMN to various electron acceptors, such as cytochrome P450s or to some anti-cancer quinone drugs. This results in generation of free radicals and toxic oxygen metabolites, which can contribute to the cytotoxicity of these c...

journal_title：Biochemical and biophysical research communications

authors： Kwaśnicka-Crawford DA,Vincent SR

更新日期：2005-10-21 00:00:00

abstract：:The cDNA coding for the signal peptide of rat liver mitochondrial aldehyde dehydrogenase was sequenced. The deduced amino acid sequence of the signal peptide was MLRAALSTARRGPRLSRLL. From this sequence an amphiphilic helix which had a high hydrophobic moment could be constructed. A comparison to the published cDNA seq...

journal_title：Biochemical and biophysical research communications

authors： Farrés J,Guan KL,Weiner H

更新日期：1988-02-15 00:00:00

abstract：:Cytochrome P450 enzymes (CYP enzymes) catalyse the initial step in biotransformation of xenobiotics like polycyclic aromatic hydrocarbons (PAHs). The marine polychaete Nereis virens has a high capacity for biotransformation of PAHs. In the present study, the complete cDNA sequences of two novel CYP genes isolated from...

journal_title：Biochemical and biophysical research communications

authors： Jørgensen A,Rasmussen LJ,Andersen O

更新日期：2005-10-28 00:00:00

abstract：:Expression of the nm23/NDP-K gene correlates with reduced metastasis in some tumors and with increased proliferation in both nontransformed and transformed cells in culture. Decreased nm23/NDP-K expression results in mitotic arrest in neuroblasts of developing Drosophila. In order to better understand the biological r...

journal_title：Biochemical and biophysical research communications

authors： Sorscher SM,Steeg P,Feramisco JR,Buckmaster C,Boss GR,Meinkoth J

更新日期：1993-08-31 00:00:00

abstract：:Localization of myoplasmic free calcium was measured in fura2-loaded single rat myocardial cells to determine whether the mechanism of norepinephrine desensitization includes redistribution of calcium. Fluorescence intensities at each pixel were quantitated by use of a photon-counting, microchannel plate camera. From ...

journal_title：Biochemical and biophysical research communications

authors： Erne P,Hermsmeyer K

更新日期：1988-02-29 00:00:00

abstract：:Membranes of HL-60 cells were shown to possess saturable binding sites for [3H]inositol(1,3,4,5)tetrakisphosphate, with nanomolar affinity (KD = 90 nM) and a density of 250 fmol/mg protein. The specificity of the binding sites for Ins(1,3,4,5)P4 was assessed by competition studies utilising a variety of inositol polyp...

journal_title：Biochemical and biophysical research communications

authors： Bradford PG,Irvine RF

更新日期：1987-12-16 00:00:00

abstract：:Lysinuric protein intolerance (LPI; MIM 222700) is an autosomal recessive disorder characterized by defective transport of cationic amino acids lysine, arginine and ornithine. The defect is localized in the basolateral membrane of polar epithelial cells of the renal tubules and intestine. The SLC7A7 (solute carrier fa...

journal_title：Biochemical and biophysical research communications

authors： Toivonen M,Mykkänen J,Aula P,Simell O,Savontaus ML,Huoponen K

更新日期：2002-03-15 00:00:00

abstract：:MicroRNAs (miRNAs) are small non-coding RNAs that are emerging as important post-transcriptional gene regulators. miR-206 is unique in that it is expressed only in skeletal muscle, including the myoblastic C2C12 cell line. In C2C12 cells, miR-206 expression was reduced dramatically after bone morphogenetic protein (BM...

journal_title：Biochemical and biophysical research communications

authors： Sato MM,Nashimoto M,Katagiri T,Yawaka Y,Tamura M

更新日期：2009-05-22 00:00:00