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Characterization of the 5' flanking region of rat glucokinase gene.

Abstract：

:The rat glucokinase (GK) gene containing the first exon was isolated and its 5' flanking region was characterized by the bacterial chloramphenicol acetyltransferase (CAT) assay. A transient expression assay with a series of 5' deletion constructs (-5.5 k to -48) of GK-CAT fusion genes indicated that the 5' flanking sequence up to nucleotide -87 was sufficient for promoter activity in adult rat hepatocytes, but its activity was much weaker than that of the SV40 enhancer/promoter. Similar promoter activity was also detected in dRLh-84 hepatoma cells, which do not express glucokinase. Insulin treatment caused no change in the CAT activity of hepatocytes transfected with the fusion genes. These results suggest that the 5' flanking region of the glucokinase gene up to -5.5 k does not contain enhancer elements responsible for tissue-specific expression or insulin regulation.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Noguchi T,Takenaka M,Yamada K,Matsuda T,Hashimoto M,Tanaka T

doi

10.1016/0006-291x(89)91803-2

subject

Has Abstract

pub_date

1989-11-15 00:00:00

pages

1247-52

issue

3

eissn

0006-291X

issn

1090-2104

pii

0006-291X(89)91803-2

journal_volume

164

pub_type

杂志文章

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