当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Differential expression of galectins in normal, benign and malignant prostate epithelial cells: silencing of galectin-3 expression in prostate cancer by its promoter methylation.

Differential expression of galectins in normal, benign and malignant prostate epithelial cells: silencing of galectin-3 expression in prostate cancer by its promoter methylation.

Abstract：

:Galectins (gal), a family of soluble beta-galactoside-binding proteins present at the cell surface, are involved in cancer progression and metastasis. Here we investigated the expression of several galectins in normal (PrEC), benign (BPH-1), and malignant (LNCaP) prostate epithelial cells and found that all galectins, except gal1 are differentially expressed. The gal3, 7, and 9 are highly expressed in PrEC, but not in LNCaP cells. Out of seven isoforms of gal8, the proto isoform gal8e and our newly discovered proto isoform gal8g were upregulated in LNCaP cells compared to PrEC, whereas the two tandem-repeat isoforms gal8a and gal8b were equally expressed in these cells. To determine if the silencing of gal3 in LNCaP cells was due to promoter methylation, LNCaP cells were treated with azacytidine. Azacytidine treatment induced the expression of gal3 in LNCaP cells, indicating that the gal3 gene was silenced by methylation of its promoter. To examine further, we evaluated cytosine methylation in gal3 promoter in LNCaP, normal prostate and placenta DNA and observed that it is highly methylated in LNCaP but not in normal cells and azacytidine completely abolished this methylation in LNCaP cells. Similar to prostate cancer cells, gal3 promoter was highly methylated in human prostate cancer tissue but not in normal tissue. To our knowledge, this is the first report indicating that gal3 expression is regulated by promoter methylation in LNCaP cells and prostate tumors. The methylation of gal3 promoter may constitute a powerful tool for early diagnosis of prostate cancer.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Ahmed H,Banerjee PP,Vasta GR

doi

10.1016/j.bbrc.2007.04.114

subject

Has Abstract

pub_date

2007-06-22 00:00:00

pages

241-6

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(07)00835-2

journal_volume

358

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Extracellular calcium-sensing receptor (CaR) expression and its potential role in parathyroid hormone-related peptide (PTHrP) secretion in the H-500 rat Leydig cell model of humoral hypercalcemia of malignancy.

    abstract：:Humoral hypercalcemia of malignancy (HHM) occurs when secretion of parathyroid hormone-related peptide (PTHrP) by cancer cells causes hypercalcemia in the absence of skeletal metastases. High extracellular calcium (Ca(2+)(o)) increases secretion of PTH-like bioactivity by rat H-500 leydig cells, a transplantable model...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.2157

    authors： Sanders JL,Chattopadhyay N,Kifor O,Yamaguchi T,Brown EM

    更新日期：2000-03-16 00:00:00

  • Nuclear translocation of human angiogenin in cultured human umbilical artery endothelial cells is microtubule and lysosome independent.

    abstract：:Exogenous angiogenin undergoes rapid nuclear translocation in cultured human umbilical artery endothelial cells at 37 degrees C but not at 4 degrees C. Treatment of cells with colchicine, nocodazole and taxol, which disrupt the microtubule system, does not affect the nuclear translocation process of angiogenin, sugges...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.7290

    authors： Li R,Riordan JF,Hu G

    更新日期：1997-09-18 00:00:00

  • Transposon-independent increase of transcription by the Sleeping Beauty transposase.

    abstract：:When a plasmid encoding the Sleeping Beauty (SB) transposase (pCMV-SB) was cointroduced with luciferase expression plasmid DNA into mouse skeletal muscle at a molar ratio of 4:1, luciferase gene expression was 5 times higher than the expression without pCMV-SB on day 28. This enhancement was not dependent on the prese...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.03.116

    authors： Masuda K,Yamamoto S,Endoh M,Kaneda Y

    更新日期：2004-05-07 00:00:00

  • Nucleophosmin/B23 regulates PCNA promoter through YY1.

    abstract：:Ectopic over-expression of nucleophosmin/B23 caused a marked up-regulation in the amounts of Yin Yang 1 (YY1) and proliferating cellular nuclear antigen (PCNA) proteins. Transfection with nucleophosmin/B23-targeting siRNA induced a decrease in the intracellular amounts of nucleophosmin/B23, YY1, and PCNA. PCNA express...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.07.150

    authors： Weng JJ,Yung BY

    更新日期：2005-09-30 00:00:00

  • Purification and NH2-terminal sequence of cytochrome P-450 from kidney microsomes of untreated male rats.

    abstract：:The major form of cytochrome P-450, P-450K-5, was purified from kidney microsomes of untreated male rats with high-performance liquid chromatography with anion-exchange and hydroxylapatite columns. The monomeric molecular weight of P-450K-5 was 52000 on SDS-polyacrylamide gel electrophoresis and the CO-reduced absorpt...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(86)80230-3

    authors： Imaoka S,Funae Y

    更新日期：1986-12-15 00:00:00

  • Delivery of ferric ion to mouse spermatozoa is mediated by lipocalin internalization.

    abstract：:The aim of this study was to illustrate the further process of 24p3 protein after association with epididymal spermatozoa. We have previously identified a caput-initiated 24p3 protein, which interacts with the spermatozoa surface in vitro. In the present study, we investigate another role of the 24p3 protein with sper...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.05.091

    authors： Elangovan N,Lee YC,Tzeng WF,Chu ST

    更新日期：2004-07-09 00:00:00

  • Two forms of AMP deaminase from chicken liver.

    abstract：:Chromatography of chicken liver AMP deaminase on phosphocellulose and DEAE-Sephacel revealed the existence of two separate peaks of enzyme activity. Significant differences have been observed between form I and II of the enzyme in respect to substrate specificity and their kinetic and regulatory properties. ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(83)90662-9

    authors： Spychała J,Makarewicz W

    更新日期：1983-08-12 00:00:00

  • Stretch-induced alterations of human Kir2.1 channel currents.

    abstract：:The inward rectifier potassium channel, Kir2.1, contributes to the I(K1) current in cardiac myocytes and is closely associated with atrial fibrillation. Strong evidences have shown that atrial dilatation or stretch may result in atrial fibrillation. However, the role of Kir2.1 channels in the stretch-mediated atrial f...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.10.049

    authors： He Y,Xiao J,Yang Y,Zhou Q,Zhang Z,Pan Q,Liu Y,Chen Y

    更新日期：2006-12-15 00:00:00

  • FBXO11 represses cellular response to hypoxia by destabilizing hypoxia-inducible factor-1α mRNA.

    abstract：:The transcriptional factor hypoxia-inducible factor-1α (HIF-1α) is induced under hypoxia and plays crucial roles in cancer progression and angiogenesis. Protein arginine methyltransferases (PRMTs), 11 isoforms of which have been identified so far, modulates the functions of diverse proteins by catalyzing arginine meth...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.07.037

    authors： Ju UI,Park JW,Park HS,Kim SJ,Chun YS

    更新日期：2015-09-04 00:00:00

  • Evidence that in situ generated reactive oxygen species act as a potent stage I tumor promoter in mouse skin.

    abstract：:A body of indirect evidence has suggested the involvement of reactive oxygen species (ROS) in tumor promotion. However, direct evidence for the involvement of in situ generated ROS in tumor promotion is lacking at present. This study provides the first in situ evidence for the involvement of ROS in stage I tumor promo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.1555

    authors： Giri U,Sharma SD,Abdulla M,Athar M

    更新日期：1995-04-17 00:00:00

  • ATR microspectroscopy with multivariate analysis segregates grades of exfoliative cervical cytology.

    abstract：:Although cervical cancer screening in the UK has led to reductions in the incidence of invasive disease, this programme remains flawed. We set out to examine the potential of infrared (IR) microspectroscopy to allow the profiling of cellular biochemical constituents associated with disease progression. Attenuated tota...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.11.005

    authors： Walsh MJ,Singh MN,Pollock HM,Cooper LJ,German MJ,Stringfellow HF,Fullwood NJ,Paraskevaidis E,Martin-Hirsch PL,Martin FL

    更新日期：2007-01-05 00:00:00

  • Inhibitory selectivity of canecystatin: a recombinant cysteine peptidase inhibitor from sugarcane.

    abstract：:The cDNA of a cystein peptidase inhibitor was isolated from sugarcane and expressed in Escherichia coli. The protein, named canecystatin, has previously been shown to exert antifungal activity on the filamentous fungus Trichoderma reesei. Herein, the inhibitory specificity of canecystatin was further characterized. It...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.06.053

    authors： Oliva ML,Carmona AK,Andrade SS,Cotrin SS,Soares-Costa A,Henrique-Silva F

    更新日期：2004-08-06 00:00:00

  • p53 independent G(1) arrest induced by DL-alpha-difluoromethylornithine.

    abstract：:Ornithine decarboxylase (ODC), which catalyzes polyamine biosynthesis, plays an essential role in cell growth. DL-alpha-Difluoromethylornithine (DFMO), a synthetic inhibitor of ODC, inhibits cell growth. However, the exact mechanism by which polyamine depletion by DFMO results in growth inhibition remains to be elucid...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.4227

    authors： Nemoto T,Kamei S,Seyama Y,Kubota S

    更新日期：2001-01-26 00:00:00

  • Dissecting the cosubstrate structure requirements of the Staphylococcus aureus aminoglycoside resistance enzyme ANT(4').

    abstract：:Aminoglycosides are important antibiotics used against a wide range of pathogens. As a mechanism of defense, bacteria have evolved enzymes able to inactivate these drugs by regio-selectively adding a variety of functionalities (acetyl, phospho, and nucelotidyl groups) to their scaffolds. The aminoglycoside nucleotidyl...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.10.119

    authors： Porter VR,Green KD,Zolova OE,Houghton JL,Garneau-Tsodikova S

    更新日期：2010-12-03 00:00:00

  • CYP4T1--a cytochrome P450 expressed in rainbow trout (Oncorhynchus mykiss) liver.

    abstract：:Total RNA isolated from a rainbow trout (Oncorhynchus mykiss) liver was subjected to RT/PCR using degenerate primers designed from homologous regions amongst cytochrome P450 CYP4 proteins. PCR amplification resulted in a single electrophoretic band which was excised, purified and sequenced directly, using cycle sequen...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.6957

    authors： Falckh PH,Wu QK,Ahokas JP

    更新日期：1997-07-18 00:00:00

  • Human kidney anion exchanger 1 interacts with adaptor-related protein complex 1 μ1A (AP-1 mu1A).

    abstract：:Kidney anion exchanger 1 (kAE1) mediates chloride (Cl⁻) and bicarbonate (HCO₃⁻) exchange at the basolateral membrane of kidney α-intercalated cells. Impaired trafficking of kAE1 leads to defect of the Cl⁻/HCO₃⁻ exchange at the basolateral membrane and failure of proton (H+) secretion at the apical membrane, causing a ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.09.015

    authors： Sawasdee N,Junking M,Ngaojanlar P,Sukomon N,Ungsupravate D,Limjindaporn T,Akkarapatumwong V,Noisakran S,Yenchitsomanus PT

    更新日期：2010-10-08 00:00:00

  • Detection of activated Caspase-3 by a cleavage site-directed antiserum during naturally occurring DRG neurons apoptosis.

    abstract：:We prepared a cleavage site-directed antiserum against Caspase-3 (anti-p20/17), which reacts with the p20/17 fragment (p20/17) activated by cleavage but not proCaspase-3 (p32), and examined the relationship between the activation of Caspase-3 and apoptosis. We identified p20/17-positive cells where cell death occurs n...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.8815

    authors： Kouroku Y,Urase K,Fujita E,Isahara K,Ohsawa Y,Uchiyama Y,Momoi MY,Momoi T

    更新日期：1998-06-29 00:00:00

  • Effect of cortisol on gonadotropin inhibitory hormone (GnIH) in the cinnamon clownfish, Amphiprion melanopus.

    abstract：:Hypothalamic peptides, gonadotropin-releasing hormone (GnRH) and gonadotropin inhibitory hormone (GnIH), play pivotal roles in the control of reproduction and gonadal maturation in fish. In the present study we tested the possibility that stress-mediated reproductive dysfunction in teleost may involve changes in GnRH ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.02.078

    authors： Choi YJ,Habibi HR,Kil GS,Jung MM,Choi CY

    更新日期：2017-04-01 00:00:00

  • Oxidation of individual fatty acids yields different profiles of oxidation markers.

    abstract：:Free radicals attack lipid molecules and initiate a chain of reactions that may trigger several diseases such as atherosclerosis and cancer. Several markers of oxidative stress have been proposed, including measurements of lipid peroxides and short-chain aldehydes levels and evaluation of conjugated diene formation. A...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.8463

    authors： Visioli F,Colombo C,Galli C

    更新日期：1998-04-17 00:00:00

  • Ras/MEK pathway is required for NGF-induced expression of tyrosine hydroxylase gene.

    abstract：:Neurotrophins are essential for the development and survival of catecholaminergic neurons. However, the critical pathway for expression of the tyrosine hydroxylase (TH) gene induced by neurotrophin is still unclear. Here we found that Ras/MEK pathway is required for NGF-induced expression of the TH gene in PC12D cells...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.01.068

    authors： Suzuki T,Kurahashi H,Ichinose H

    更新日期：2004-03-05 00:00:00

  • Genetic imbalances in preleukemic thymuses.

    abstract：:To understand the molecular mechanisms involved in preleukemia, the suppression subtractive hybridization method was used in a murine radiation-induced thymic lymphoma model. Seventeen mRNAs overexpressed in preleukemic thymuses were identified: mouse laminin binding protein (p40/37LBP), E25 protein, Rattus norvegicus...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.4731

    authors： Verlaet M,Deregowski V,Denis G,Humblet C,Stalmans MT,Bours V,Castronovo V,Boniver J,Defresne MP

    更新日期：2001-04-27 00:00:00

  • Establishment of a new glial cell line from hippocampus of prion protein gene-deficient mice.

    abstract：:Cellular prion protein (PrP(C)) is expressed not only in neuronal cells but also in non-neuronal cells such as astroglial cells. In the present study, the prion protein (PrP) gene (Prnp)-deficient astroglial cell line GpL1 from hippocampal cells of ZrchI Prnp(-/-) mice were established. Transfection of Prnp suppressed...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.10.087

    authors： Nishimura T,Sakudo A,Xue G,Ikuta K,Yukawa M,Sugiura K,Onodera T

    更新日期：2008-12-26 00:00:00

  • Parallel induction of nitric oxide and glucose-6-phosphate dehydrogenase in activated bone marrow derived macrophages.

    abstract：:The production of nitric oxide (NO.) and the induction of glucose-6-phosphate dehydrogenase by lipopolysaccharides (LPS) from different sources was studied in bone marrow derived macrophages (BMM phi). NO. production was found to be linked to the induction of glucose-6-phosphate dehydrogenase, suggesting the possible ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.2254

    authors： Corraliza IM,Campo ML,Fuentes JM,Campos-Portuguez S,Soler G

    更新日期：1993-10-15 00:00:00

  • 2-Deoxy-d-glucose increases GFAT1 phosphorylation resulting in endoplasmic reticulum-related apoptosis via disruption of protein N-glycosylation in pancreatic cancer cells.

    abstract：:The glycolytic inhibitor 2-deoxy-d-glucose (2DG) causes energy starvation, affecting cell viability in a wide range of cancer cell lines. To determine the action of 2DG in pancreatic cancer, we performed proteomic analysis of pancreatic cancer cell line after 2DG treatment. Eighty proteins showed differential expressi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.05.041

    authors： Ishino K,Kudo M,Peng WX,Kure S,Kawahara K,Teduka K,Kawamoto Y,Kitamura T,Fujii T,Yamamoto T,Wada R,Naito Z

    更新日期：2018-06-27 00:00:00

  • The potentiation by TNF-alpha and PMA of Fc receptor-mediated phagocytosis in neutrophils is independent of reactive oxygen metabolites produced by NADPH oxidase and of protein kinase C.

    abstract：:The results presented in this paper demonstrate that the potentiation of phagocytosis of erythrocyte (E) IgG by TNF-alpha or PMA is not due to an oxygen-dependent mechanism. In fact, the potentiation of phagocytosis occurs normally in human neutrophils 1) when the respiratory burst is inhibited by diphenyleneiodonium,...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.1713

    authors： Della Bianca V,Grzeskowiak M,Renzi E,Rossi F

    更新日期：1993-06-30 00:00:00

  • Neonatal growth cartilage: equine tissue specific gene expression.

    abstract：:Endochondral bone formation is an important process in development and growth of the skeleton; still many of the mechanisms of growth cartilage remain unknown. The aim of this study was to identify genes specifically expressed in growth cartilage by constructing a subtraction cDNA library of the articular-epiphyseal c...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.01.074

    authors： Johannessen MK,Skretting G,Ytrehus B,Røed KH

    更新日期：2007-03-23 00:00:00

  • LncRNA TP73-AS1 predicts the prognosis of bladder cancer patients and functions as a suppressor for bladder cancer by EMT pathway.

    abstract：:Long noncoding RNAs (lncRNAs) have been identified to have more and more important roles in tumorigenesis and may be novel biomarker for cancer therapy. LncRNA TP73-AS1 is a novel identified lncRNA that has been demonstrated to be increased in several cancers, however, its function in bladder cancer remains unknown. T...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.04.010

    authors： Tuo Z,Zhang J,Xue W

    更新日期：2018-05-23 00:00:00

  • Evidence of ABL-kinase domain mutations in highly purified primitive stem cell populations of patients with chronic myelogenous leukemia.

    abstract：:To study the hierarchical levels of stem cell targets for ABL-kinase domain mutations in CML, highly purified CD34+CD38- and CD34+CD38+ cell populations and their LTC-IC-derived progeny were analyzed in four patients at diagnosis (n=1) or in advanced phases (n=3) of their disease. In the single patient with early phas...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.08.169

    authors： Sorel N,Bonnet ML,Guillier M,Guilhot F,Brizard A,Turhan AG

    更新日期：2004-10-22 00:00:00

  • DNA repair in lymphocytes from humans and rats with chronic iron overload.

    abstract：:A marked reduction of the proliferative capability after a mitogenic stimulus and a dramatic decrease of the capacity to repair DNA damages were found in lymphocytes from iron overloaded rats. These immunological parameters were not significantly different from controls in peripheral blood lymphocytes from patients wi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(88)90196-9

    authors： Pietrangelo A,Cossarizza A,Monti D,Ventura E,Franceschi C

    更新日期：1988-07-29 00:00:00

  • Ribosomal protein methyltransferases in the yeast Saccharomyces cerevisiae: Roles in ribosome biogenesis and translation.

    abstract：:A significant percentage of the methyltransferasome in Saccharomyces cerevisiae and higher eukaryotes is devoted to methylation of the translational machinery. Methylation of the RNA components of the translational machinery has been studied extensively and is important for structure stability, ribosome biogenesis, an...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.01.107

    authors： Al-Hadid Q,White J,Clarke S

    更新日期：2016-02-12 00:00:00