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Quantitative analysis of the transcription factor AP2 binding to DNA by atomic force microscopy.

Abstract：

:Atomic force microscopy (AFM) allows to study the molecular structure of biological macromolecules with nm to A resolutions without crystallization. We show here the applicability of AFM in the quantitative analysis of the molecular mechanisms of DNA/protein interaction: (i) Protein-binding sites can be mapped over a few kilobases of target DNA. (ii) Multimerization state of DNA-binding proteins can be determined simply by measuring the sizes of proteins bound to the DNA. These features are significant advantages over the capabilities provided by conventional techniques in biochemistry and molecular and structural biology.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Nettikadan S,Tokumasu F,Takeyasu K

doi

10.1006/bbrc.1996.1409

subject

Has Abstract

pub_date

1996-09-24 00:00:00

pages

645-9

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(96)91409-6

journal_volume

226

pub_type

杂志文章

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