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Conjugal transfer of plasmid and transposon DNA from Escherichia coli into Porphyromonas gingivalis.

Abstract：

:Using the broad host-range vector R751 to provide transfer functions, plasmid pVAL-1 and transposon Tn4351 were conjugally mobilized from Escherichia coli into Porphyromonas gingivalis. Transfer frequencies for both elements varied between 10(-6) and 10(-11), depending upon the recipient. The behavior of pVAL-1 and Tn4351 in P. gingivalis was essentially as described previously in Bacteroides spp. These data indicate that plasmid and transposon DNA can be conjugally transferred into P. gingivalis and that these elements can be used to genetically manipulate the organism in examining putative virulence determinants that may participate in the induction or exacerbation of periodontal disease.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Dyer DW,Bilalis G,Michel JH,Malek R

doi

10.1016/0006-291x(92)90847-e

subject

Has Abstract

pub_date

1992-07-31 00:00:00

pages

1012-9

issue

2

eissn

0006-291X

issn

1090-2104

pii

0006-291X(92)90847-E

journal_volume

186

pub_type

杂志文章

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