Titration of variant DNA sequences differing by a single point-mutation by selective dot-blot hybridization with synthetic oligonucleotides.

abstract：:A system for the expression and purification of soluble VP8*, part of the human rotavirus (HRV) spike protein, was established by expressing VP8* as a fusion protein with glutathione S-transferase (GST). VP8 cDNA, from the Wa strain of HRV, was prepared by RT-PCR, cloned into a pUC18 plasmid, and inserted into a pGEX-...

journal_title：Biochemical and biophysical research communications

authors： Kovacs-Nolan J,Sasaki E,Yoo D,Mine Y

更新日期：2001-04-20 00:00:00

abstract：:The effect of the length of ANF peptides on the inhibition of the norepinephrine-induced contraction was studied. Starting from the 26 residues ANF (Arg101-Tyr126), shorter N- and/or C-terminal fragments were produced, either by N-terminal chemical cleavage or C-terminal enzymatic digestion of ANF or both respectively...

journal_title：Biochemical and biophysical research communications

authors： Garcia R,Thibault G,Seidah NG,Lazure C,Cantin M,Genest J,Chrétien M

更新日期：1985-01-16 00:00:00

abstract：:Although Pol III promoters synthesize shRNA and elicit RNAi efficiently, however, a major limitation is that they are constitutively expressed in all cell types. To circumvent this problem, in the present study, we described a novel shRNA vector based on Pol II/T7 dual-promoter couple system: the transcription of shRN...

journal_title：Biochemical and biophysical research communications

authors： Peng Y,Lu JX,Shen XF

更新日期：2007-08-24 00:00:00

abstract：:Using in vitro assays, we show that nuclear proteins related to the Sp1 and GT-1 factors bind to a CACCC box sequence in the human beta-globin enhancer, adjacent to binding sites for the erythroid-specific factor NFE1 and the ubiquitous factor CP1. The same proteins are known to bind to the proximal, but not to the di...

journal_title：Biochemical and biophysical research communications

authors： Giglioni B,Comi P,Ronchi A,Mantovani R,Ottolenghi S

更新日期：1989-10-16 00:00:00

abstract：:Aminopeptidase A (APA; EC 3.4.11.7) is a transmembrane metalloprotease with several functions in tumor angiogenesis. To investigate the role of APA in the process of ischemia-induced angiogenesis, we evaluated the cellular angiogenic responses under hypoxic conditions and the process of perfusion recovery in the hindl...

journal_title：Biochemical and biophysical research communications

authors： Kubota R,Numaguchi Y,Ishii M,Niwa M,Okumura K,Naruse K,Murohara T

更新日期：2010-11-12 00:00:00

abstract：:Newly translated proteins must undergo proper folding to ensure their function. To enter a low energy state, misfolded proteins form aggregates, which are associated with many degenerative diseases, such as Huntington's disease and chronic kidney disease (CKD). Recent studies have shown the use of low molecular weight...

journal_title：Biochemical and biophysical research communications

authors： Upagupta C,Carlisle RE,Dickhout JG

更新日期：2017-04-22 00:00:00

abstract：:Cardiac hypertrophy is a major predictor of heart failure and of morbidity and mortality in developed countries. Many hormones and growth factors induce cardiac hypertrophy via activation of members of the phospholipase C (PLC) family. The expression pattern of the PLCbeta isozyme subfamily was investigated in neonata...

journal_title：Biochemical and biophysical research communications

authors： Schnabel P,Mies F,Nohr T,Geisler M,Böhm M

更新日期：2000-08-18 00:00:00

abstract：:The identification and mapping of genes within the Down syndrome region is an important step toward a complete understanding of the pathogenesis of this disorder. The objective of the present work is to identify and map genes within the Down syndrome region-2. Chromosome 21 cosmid clones corresponding to "cosmid pocke...

journal_title：Biochemical and biophysical research communications

authors： Vidal-Taboada JM,Bergoñón S,Sánchez M,López-Acedo C,Groet J,Nizetic D,Egeo A,Scartezzini P,Katsanis N,Fisher EM,Delabar JM,Oliva R

更新日期：1998-02-13 00:00:00

abstract：:GOTO cells, a neuroblastoma cell line retaining the ability to differentiate into neuronal or Schwann cells, were found to be rich in membrane rafts containing ganglioside GM2 and hypersensitive to lipid raft-disrupting methyl-beta-cyclodextrin (MbetaCD); the GM2-rich rafts and sensitivity to MbetaCD were markedly dim...

journal_title：Biochemical and biophysical research communications

authors： Tomioka R,Minami N,Kushida A,Horibe S,Izumi I,Kato A,Fukushima K,Ideo H,Yamashita K,Hirose S,Saito Y

更新日期：2009-11-06 00:00:00

abstract：:L-arginine may aid in the liver detoxification and may benefit in the treatment of liver disorders such as liver injury. The present study was to investigate the possible protective and curative effects of L-arginine on carbon tetrachloride (CCl(4)) induced hepatotoxicity. Mice received a single dose of CCl(4). L-argi...

journal_title：Biochemical and biophysical research communications

authors： Saad EA

更新日期：2012-06-22 00:00:00

abstract：:The clottable protein (CP) was isolated from white shrimp, Penaeus vannamei plasma as a 400-kDa protein that splits to two identical 200-kDa subunits when it is reduced with 2-ME. However, using DTT as reducing agent, four main bands were observed; two of them (179 and 125 kDa) had the same N-terminus sequence of the ...

journal_title：Biochemical and biophysical research communications

authors： Reyes-Izquierdo T,Vargas-Albores F

更新日期：2001-09-21 00:00:00

abstract：:Identifying Mycobacterium tuberculosis membrane proteins involved in binding to and invasion of host cells is important in designing subunit-based anti-tuberculosis vaccines. The Rv2969c gene sequence was identified by PCR in M. tuberculosis complex strains, being transcribed in M. tuberculosis H37Rv, M. tuberculosis ...

journal_title：Biochemical and biophysical research communications

authors： Patarroyo MA,Plaza DF,Ocampo M,Curtidor H,Forero M,Rodriguez LE,Patarroyo ME

更新日期：2008-08-08 00:00:00

abstract：:Administration of tryptophan or hydrocortisone to rats caused a several-fold increase in tryptophan-2,3-dioxygenase activity in the liver. Highly purified lysosomes were isolated from livers of tryptophan- or hydrocortisone-treated animals as well as the control rats. Immunoblotting of lysosomal proteins with anti-try...

journal_title：Biochemical and biophysical research communications

authors： Sato A,Endo Y,Natori Y

更新日期：1992-02-28 00:00:00

abstract：:The proliferation of colon cancer cells is mediated in part by epidermal growth factor receptor (EGFR) signaling and requires sustained levels of cellular energy to meet its high metabolic needs. Intracellular lipid droplets (LDs) are a source of energy used for various cellular functions and they are elevated in dens...

journal_title：Biochemical and biophysical research communications

authors： Penrose H,Heller S,Cable C,Makboul R,Chadalawada G,Chen Y,Crawford SE,Savkovic SD

更新日期：2016-01-15 00:00:00

abstract：:To explore the pathophysiologic roles of the obese (ob) gene product, leptin, in the development of obesity and hypertension, we examined ob gene expression and leptin secretion in obese spontaneously hypertensive rats (obese SHR or Koletsky rats) at the stage of established obesity and hypertension. Expression of the...

journal_title：Biochemical and biophysical research communications

authors： Hiraoka J,Hosoda K,Ogawa Y,Ikeda K,Nara Y,Masuzaki H,Takaya K,Nakagawa K,Mashimo T,Sawamura M,Koletsky RJ,Yamori Y,Nakao K

更新日期：1997-02-24 00:00:00

abstract：:The 15-kDa selenoprotein (Sep15) has been implicated in etiology of some types of cancer. Herein, inducible RNAi cell lines were established and cell morphology and motility were analyzed. The majority of Sep15-deficient cells (>95%) formed membrane blebs in a dynamic manner. Blebbing cells transformed cell morphology...

journal_title：Biochemical and biophysical research communications

authors： Bang J,Jang M,Huh JH,Na JW,Shim M,Carlson BA,Tobe R,Tsuji PA,Gladyshev VN,Hatfield DL,Lee BJ

更新日期：2015-01-24 00:00:00

abstract：:Runt-related transcription factor-2 (RUNX2) is expressed as two isoforms (type-I and type-II) differing only in their amino terminal sequences. The amino terminus of type-I contains MRIPV instead of MASNSLFSAVTPCQQSFFW in type-II. Although type-II mRNA has been considered osteoblast specific, the RUNX2 protein isoform...

journal_title：Biochemical and biophysical research communications

authors： Sudhakar S,Katz MS,Elango N

更新日期：2001-08-10 00:00:00

abstract：:Each chromosome occupies its own-specific space called a 'territory' within the interphase nucleus, and the arrangement of chromosome territories (CTs) is important in epigenetic mechanisms. The molecular mechanism to determine the positioning of CTs, however, remains unknown. On the other hand, dioxin is known to be ...

journal_title：Biochemical and biophysical research communications

authors： Oikawa K,Yoshida K,Takanashi M,Tanabe H,Kiyuna T,Ogura M,Saito A,Umezawa A,Kuroda M

更新日期：2008-09-19 00:00:00

abstract：:The effects of chlorpromazine on either the activity of mouse brain nitric oxide synthase or the induction of lung nitric oxide synthase in mice and rats were studied. Chlorpromazine inhibited the nitric oxide synthase activity in mouse brain cytosol. This effect could be reversed by adding an excess of calmodulin. In...

journal_title：Biochemical and biophysical research communications

authors： Palacios M,Padron J,Glaria L,Rojas A,Delgado R,Knowles R,Moncada S

更新日期：1993-10-15 00:00:00

abstract：:Previously by selection and inbreeding of Wistar rats susceptible or resistant to the cataractogenic effect of galactose the S and R rat strains were developed as a model of oxidative stress. It was earlier found S rats have elevated level of OH-radical generation and enhanced lipid peroxidation compared with that for...

journal_title：Biochemical and biophysical research communications

authors： Yelinova V,Glazachev Y,Khramtsov V,Kudryashova L,Rykova V,Salganik R

更新日期：1996-04-16 00:00:00

abstract：:Simultaneous patch-clamp and Ca2+ fluorescence measurements have revealed depolarising oscillations in the membrane potential of arterial (pulmonary) myocytes in response to adenosine 5'-triphosphate (ATP) and endothelin-1 (ET-1). These oscillations (i) are due to the preferential activation of Ca(2+)-activated Cl-, o...

journal_title：Biochemical and biophysical research communications

authors： Bakhramov A,Hartley SA,Salter KJ,Kozlowski RZ

更新日期：1996-10-03 00:00:00

abstract：:We investigated the genomic organization of pancreatic zymogen granule membrane-associated protein GP2, a GPI-anchored protein exhibiting self-aggregation at acidic pH, in order to construct a gene-knockout mouse. Cloning and analysis of lambda clones encoding GP2 from 129 Svj mouse genomic DNA libraries showed that t...

journal_title：Biochemical and biophysical research communications

authors： Kobayashi K,Yanagihara K,Ishiguro K,Fukuoka S

更新日期：2004-09-17 00:00:00

abstract：:Production of endothelin-1 in the cultured neonatal rat cardiac myocytes was investigated. Studies on the cultured cardiac myocytes with reverse transcription-polymerase chain reaction followed by Southern hybridization indicated that mRNA of ET-1(preproET-1 mRNA) was expressed by cardiac myocytes. ET-1 polypeptide wa...

journal_title：Biochemical and biophysical research communications

authors： Suzuki T,Kumazaki T,Mitsui Y

更新日期：1993-03-31 00:00:00

abstract：:Myeloperoxidase (MPO) is a glycosylated heme-containing enzyme present in the azurophilic granules of normal human polymorphonuclear neutrophils. This enzyme plays a major role in the microbicidal activity of the host defense system by catalyzing the formation of the potent oxidant, hypochlorous acid. Although the ami...

journal_title：Biochemical and biophysical research communications

authors： Taylor KL,Uhlinger DJ,Kinkade JM Jr

更新日期：1992-09-30 00:00:00

abstract：:The effects of different lysophospholipids on endothelium-dependent hyperpolarization by acetylcholine were examined in rat mesenteric arteries. Lysophosphatidylcholine with 14 or longer carbon acyl chain significantly inhibited the hyperpolarization, while that with 12 or lesser carbon acyl chain was without effect. ...

journal_title：Biochemical and biophysical research communications

authors： Fukao M,Hattori Y,Kanno M,Sakuma I,Kitabatake A

更新日期：1996-10-14 00:00:00

abstract：:Treatment of newborn murine epidermal cells with phospholipase C results in the generation of a chemiluminescence response similar to that previously described for phorbol ester tumor promoters. Based on inhibitor studies, the oxidant, believed to be superoxide anion, is most likely generated from lipoxygenase metabol...

journal_title：Biochemical and biophysical research communications

authors： Fischer SM,Baldwin JK,Adams LM

更新日期：1985-09-30 00:00:00

abstract：:Sodium dodecyl sulfate (SDS), a representative anionic surfactant, is a commonly used reagent in studies of the cell membrane and cell wall. However, the mechanisms through which SDS affects cellular functions have not yet been fully examined. Thus, to gain further insights into the cellular functions and responses to...

journal_title：Biochemical and biophysical research communications

authors： Zhao F,Yang J,Li J,Li Z,Lin Y,Zheng S,Liang S,Han S

更新日期：2020-06-18 00:00:00

abstract：:Nerve growth factor (NGF) elicits Akt translocation into the nucleus, where it phosphorylates nuclear targets. Here, we describe that Akt phosphorylation can promote the nuclear translocation of Akt and is necessary for its nuclear retention. Overexpression of Akt-K179A, T308A, S473A-mutant failed to show either nucle...

journal_title：Biochemical and biophysical research communications

authors： Xuan Nguyen TL,Choi JW,Lee SB,Ye K,Woo SD,Lee KH,Ahn JY

更新日期：2006-10-20 00:00:00

abstract：:Astrocyte elevated gene-1 (AEG-1), upregulated in various types of malignancies including colorectal cancer (CRC), has been reported to be associated with the carcinogenesis. MicroRNAs (miRNAs) are widely involved in the initiation and progression of cancer. However, the functional significance of AEG-1 and the relati...

journal_title：Biochemical and biophysical research communications

authors： Huang S,Wu B,Li D,Zhou W,Deng G,Zhang K,Li Y

更新日期：2014-02-14 00:00:00

abstract：:The ability of phospholipase C inhibitors to inhibit Ca2+ channel stimulation and Ca2+ release from intracellular stores evoked by norepinephrine in single rat portal vein myocytes was investigated in the aim of identifying the type of phospholipase C involved in the transduction pathways activated by alpha 1A- and al...

journal_title：Biochemical and biophysical research communications

authors： Macrez-Leprêtre N,Morel JL,Mironneau J

更新日期：1996-01-05 00:00:00