Phospholipase C mimics tumor promoter-induced chemiluminescence in murine epidermal cells.

abstract：:Exposure of rat atrial slices to 10(-5) M norepinephrine (NE) for 10 minutes increases 45Ca++ accumulation from 1.64 +/- 0.10 to 2.23 +/- 0.06 nmol/mg tissue. In the presence of leucine enkephalin (10(-8) M), NE-stimulated 45Ca++ uptake is reduced to 1.44 +/- 0.10 nmol/mg tissue. The effect of leu-enkephalin is revers...

journal_title：Biochemical and biophysical research communications

authors： Ruth JA,Cuizon JV,Eiden LE

更新日期：1983-12-16 00:00:00

abstract：:Endo-beta-1,4-xylanase X-I is a major hydrolase produced by the aleurone tissue of germinating barley grain. It was previously reported that this cytosolic enzyme is synthesized as an inactive precursor which is proteolytically processed to active forms upon its programmed cell death dependent release. We here demonst...

journal_title：Biochemical and biophysical research communications

authors： Van Campenhout S,Pollet A,Bourgois TM,Rombouts S,Beaugrand J,Gebruers K,De Backer E,Courtin CM,Delcour JA,Volckaert G

更新日期：2007-05-11 00:00:00

abstract：:The Crk and CrkL adaptor proteins have SH2 and SH3 domains and play essential overlapping, as well as distinct, roles in many biological processes, ranging from cell structure and motility to proliferation. Conditional ablation of both Crk and CrkL in neuronal progenitor cells, using a Nestin-Cre transgene, resulted i...

journal_title：Biochemical and biophysical research communications

authors： Park T,Curran T

更新日期：2020-08-27 00:00:00

abstract：:The conversion of androgens to estrogens by CYP19 (cytochrome P450AROM, aromatase) is an important step in the mechanism of androgen action in the brain. CYP19 expression has been demonstrated in various animal species, but studies in human postnatal brain tissue are lacking. Therefore, we investigated CYP19 mRNA expr...

journal_title：Biochemical and biophysical research communications

authors： Stoffel-Wagner B,Watzka M,Steckelbroeck S,Schwaab R,Schramm J,Bidlingmaier F,Klingmüller D

更新日期：1998-03-27 00:00:00

abstract：:Processing of 37 precursor maltose-binding protein (preMBP) species by purified signal peptidase I (SPase I) was assayed. The in vitro reaction was inefficient compared to processing in Escherichia coli cells. The extent of preMBP processing in vitro was higher when SPase I was present during translation as compared t...

journal_title：Biochemical and biophysical research communications

authors： Talarico TL,Barkocy-Gallagher GA,Ray PH,Bassford PJ Jr

更新日期：1993-12-30 00:00:00

abstract：:In virus-infected cells, viral RNA with non-self structural pattern is recognized by DExD/Hbox RNA helicase, RIG-I. Once RIG-I senses viral RNA, it triggers a signaling cascade, resulting in the activation of genes including type I interferon, which activates antiviral responses. Overexpression of N-terminal caspase a...

journal_title：Biochemical and biophysical research communications

authors： Kageyama M,Takahasi K,Narita R,Hirai R,Yoneyama M,Kato H,Fujita T

更新日期：2011-11-11 00:00:00

abstract：:Based on a homology search with endostatin, the C-terminus 185 aa of collagen XVIII, we report the cloning, expression, and antiangiogenic activity of a 22 kDa human collagen XV fragment, that we have named restin. Restin was expressed in the prokaryotic pET expression system. We have shown that restin inhibits the mi...

journal_title：Biochemical and biophysical research communications

authors： Ramchandran R,Dhanabal M,Volk R,Waterman MJ,Segal M,Lu H,Knebelmann B,Sukhatme VP

更新日期：1999-02-24 00:00:00

abstract：:A series of C-terminal linear peptides of endothelin (ET)-1 and their N alpha-succinyl (Suc) analogs were synthesized and their binding affinities for the two subtypes of ET receptor, ETA and ETB, in porcine lung membranes were examined. Among the synthetic analogs, Suc-[Glu9,Ala11,15]-ET-1(8-21), IRL 1620, was the mo...

journal_title：Biochemical and biophysical research communications

authors： Takai M,Umemura I,Yamasaki K,Watakabe T,Fujitani Y,Oda K,Urade Y,Inui T,Yamamura T,Okada T

更新日期：1992-04-30 00:00:00

abstract：:ZinT (B1973), previously known as YodA, was originally characterised as a cadmium-induced periplasmic protein under the regulation of Fur and SoxS. Here we describe a decrease in zinT transcript in response to elevated copper concentrations and the zinc and copper dependent phenotype of a DeltazinT strain. Cadmium sen...

journal_title：Biochemical and biophysical research communications

authors： Kershaw CJ,Brown NL,Hobman JL

更新日期：2007-12-07 00:00:00

abstract：:The mode of action of dihydroxyanthraquinone, a new antitumor drug, on eucaryotic chromosome structure and function was investigated using simian virus 40 as a model system. Dihydroxyanthraquinone specifically inhibited initiation of viral replicons. Little or no viral DNA synthesis was recovered in cells after the re...

journal_title：Biochemical and biophysical research communications

authors： Su RT,Ma XJ,Cheng CC

更新日期：1985-07-16 00:00:00

abstract：:The discovery of clock genes revealed the major molecular components responsible for circadian time-keeping in mammals, but the mechanism by which autonomic nervous system may control circadian rhythm and its relationship to metabolism is unclear. As the Cry1 and Cry2 genes are indispensable for molecular core oscilla...

journal_title：Biochemical and biophysical research communications

authors： Ikeda H,Yong Q,Kurose T,Todo T,Mizunoya W,Fushiki T,Seino Y,Yamada Y

更新日期：2007-12-21 00:00:00

abstract：:Recently, copper (Cu) was found to be unusually accumulated, suggesting the induction of metallothionein (MT) in the liver of LEC rats (Long-Evans rats with a cinnamon-like coat color), which develop spontaneous jaundice with hereditary hepatitis. Thus, the direct relationship between the unusual Cu accumulation and t...

journal_title：Biochemical and biophysical research communications

authors： Sakurai H,Kamada H,Fukudome A,Kito M,Takeshima S,Kimura M,Otaki N,Nakajima K,Kawano K,Hagino T

更新日期：1992-06-15 00:00:00

abstract：:Lanosterol synthase [(S)-2,3-epoxysqualene mutase (cyclizing, lanosterol forming), EC 5.4.99.7] catalyzes the cyclization of (S)-2,3-oxidosqualene to lanosterol in the reaction that forms the sterol nucleus. We report herein the cloning and characterization of the human gene (OSC) encoding lanosterol synthase, a predi...

journal_title：Biochemical and biophysical research communications

authors： Baker CH,Matsuda SP,Liu DR,Corey EJ

更新日期：1995-08-04 00:00:00

abstract：:Computing the ligand-protein binding affinity (or the Gibbs free energy) with chemical accuracy has long been a challenge for which many methods/approaches have been developed and refined with various successful applications. False positives and, even more harmful, false negatives have been and still are a common occu...

journal_title：Biochemical and biophysical research communications

authors： Villarreal OD,Yu L,Rodriguez RA,Chen LY

更新日期：2017-01-29 00:00:00

abstract：:The root-knot nematode (RKN) Meloidogyne incognita reproduces on the roots of tobacco (Nicotiana tabacum), damaging crops, reducing crop yield, and causing economic losses annually. The development of resistant genotypes is an alternative strategy to effectively control these losses. However, the molecular mechanism r...

journal_title：Biochemical and biophysical research communications

authors： Xing X,Li X,Zhang M,Wang Y,Liu B,Xi Q,Zhao K,Wu Y,Yang T

更新日期：2017-01-22 00:00:00

abstract：:Cells released from quiescence exhibit increased levels of the DNA-modifying enzyme topoisomerase II, a nuclear protein which is also a target for antitumour drugs such as VP-16 (etoposide) and m-AMSA (4',9'-acridinylamino-methanesulfon-m-anisidide). By using Western blotting, DNA-protein crosslinking and drug-induced...

journal_title：Biochemical and biophysical research communications

authors： Epstein RJ,Smith PJ

更新日期：1989-04-14 00:00:00

abstract：:Vascular calcification increases the risk of developing cardiovascular disease, and it is closely associated with metabolic disorders such as diabetes mellitus and non-alcoholic fatty liver disease. We investigated whether the activators of AMP-activated protein kinase (AMPK), metformin, resveratrol, and exendin-4, im...

journal_title：Biochemical and biophysical research communications

authors： Lee J,Hong SW,Kim MJ,Kwon H,Park SE,Rhee EJ,Lee WY

更新日期：2020-09-17 00:00:00

abstract：:This study describes the purification and characterization of an aminopeptidase from human cerebrospinal fluid capable of degrading delta-sleep-inducing-peptide (DSIP). The enzyme has an apparent molecular weight of approximately 80,000 dalton. It is sensitive towards amastatin, bestatin and EDTA and is optimally acti...

journal_title：Biochemical and biophysical research communications

authors： Nyberg F,Thörnwall M,Hetta J

更新日期：1990-03-30 00:00:00

abstract：:The suppressor of cytokine signalling 1 protein (SOCS-1) belongs to a novel family of cytokine inducible factors which function as inhibitors of the JAK/STAT pathway. While SOCS-1 previously has been described as a single-exon gene, here we present evidence for an additional 5' exon, separated by a 509 bp intron from ...

journal_title：Biochemical and biophysical research communications

authors： Schlüter G,Boinska D,Nieman-Seyde SC

更新日期：2000-02-16 00:00:00

abstract：:The highly reactive electrophile, methylglyoxal (MG), a break down product of carbohydrates, is a major environmental mutagen having potential genotoxic effects. Previous studies have suggested the reaction of MG with free amino groups of proteins forming advanced glycation end products (AGEs). This results in the gen...

journal_title：Biochemical and biophysical research communications

authors： Ahmad S,Moinuddin,Dixit K,Shahab U,Alam K,Ali A

更新日期：2011-04-15 00:00:00

abstract：:Within 4 h following the addition of 3,3',5 triiodo-L-thyronine to monolayer cultures of hepatocytes isolated from hypothyroid rats, a very distinct stimulation of fatty acid and cholesterol synthesis, measured as incorporation of either [1-14C]acetate or [3H]H2O into these lipid fractions, is observed. A smaller but ...

journal_title：Biochemical and biophysical research communications

authors： Gnoni GV,Geelen MJ,Bijleveld C,Quagliariello E,van den Bergh SG

更新日期：1985-04-30 00:00:00

abstract：:Betulinic acid (BA) exhibits various biological activities such as anti-bacterial, anti-inflammatory, anti-human papilloma virus (HPV), and anti-cancer activities. HPV infection is associated with a high risk of cervical cancer, which is the leading cause of deaths among women worldwide. Therefore, BA is an attractive...

journal_title：Biochemical and biophysical research communications

authors： Kim HJ,Cho HS,Ban HS,Nakamura H

更新日期：2020-03-12 00:00:00

abstract：:Corneal epithelial stem cells are believed to reside in the basal layer of the limbal epithelium, but no definitive cell surface markers have been identified. For keratinocytes, stem/progenitor cells are known to be enriched by cell surface markers, integrin alpha(6) and CD71, as a minor subpopulation which shows high...

journal_title：Biochemical and biophysical research communications

authors： Hayashi R,Yamato M,Saito T,Oshima T,Okano T,Tano Y,Nishida K

更新日期：2008-03-07 00:00:00

abstract：:Although TRPV1 and TRPM8 agonists (vanilloid capsaicin and menthol, respectively) at high concentrations inhibit action potential conduction, it remains to be unknown whether TRPA1 agonists have a similar action. The present study examined the actions of TRPA1 agonists, cinnamaldehyde (CA) and allyl isothiocyanate (AI...

journal_title：Biochemical and biophysical research communications

authors： Matsushita A,Ohtsubo S,Fujita T,Kumamoto E

更新日期：2013-04-26 00:00:00

abstract：:Rex-1 is a zinc finger-containing gene that is expressed in embryonal carcinoma (EC) cells and embryonic stem (ES) cells. Upon differentiation with retinoic acid (RA), transcription of the Rex-1 gene decreases rapidly. Analysis of the 5'-flanking region of the Rex-1 gene identified a consensus motif for the octamer fa...

journal_title：Biochemical and biophysical research communications

authors： Rosfjord E,Rizzino A

更新日期：1994-09-30 00:00:00

abstract：:Screening for inhibitors of DNA-dependent protein kinase (DNA-PK) revealed 3-cyano-5-(4-pyridyl)-6-hydrazonomethyl-2-pyridone, designated OK-1035, to be a potent and selective inhibitor. When a synthetic peptide was used as a substrate, OK-1035 caused 50% inhibition of DNA-PK activity at 8 microM, a concentration more...

journal_title：Biochemical and biophysical research communications

authors： Take Y,Kumano M,Hamano Y,Fukatsu H,Teraoka H,Nishimura S,Okuyama A

更新日期：1995-10-04 00:00:00

abstract：:A low molecular weight protein (approximately 25,000 D) exhibiting a yellow fluorescence emission peaking at approximately 540 nm was isolated from Vibrio fischeri (strain Y-1) and purified to apparent homogeneity. FMN is the chromophore, but it exhibits marked red shifts in both the absorption (lambda max = 380, 460 ...

journal_title：Biochemical and biophysical research communications

authors： Macheroux P,Schmidt KU,Steinerstauch P,Ghisla S,Colepicolo P,Buntic R,Hastings JW

更新日期：1987-07-15 00:00:00

abstract：:An intein is a polypeptide that interrupts the functional domains of a protein, called the exteins. The intein can facilitate its own excision from the exteins, concomitant with the ligation of the exteins, in a process called protein splicing. The alpha subunit of the ribonucleotide reductase of the extreme thermophi...

journal_title：Biochemical and biophysical research communications

authors： Kerrigan AM,Powers TL,Dorval DM,Reitter JN,Mills KV

更新日期：2009-09-11 00:00:00

abstract：:We examined how growth hormone-releasing factor (GRF) regulated pituitary GRF receptor gene expression in the conscious rat. GRF receptor mRNA levels were significantly increased by immunoneutralization of endogenous GRF with its specific antiserum. This effect was dose dependent and the maximum level was 3.8-fold hig...

journal_title：Biochemical and biophysical research communications

authors： Miki N,Ono M,Murata Y,Ohsaki E,Tamitsu K,Yamada M,Demura H

更新日期：1996-07-16 00:00:00

abstract：:We previously demonstrated several nicotinic acetylcholine receptor (nAChR) subunits and associated proteins in human sperm. Here, we identified in sperm for the first time two additional nAChR-associated molecules: (1) agrin(SN)Z(+) in human sperm localized in the posterior post-acrosomal, neck, and flagellar mid-pie...

journal_title：Biochemical and biophysical research communications

authors： Kumar P,Ferns MJ,Meizel S

更新日期：2006-04-07 00:00:00