Binding site of the rat liver specific monoclonal antibody.

abstract：:Hedgehog (Hh) signaling plays key roles in animal development and tissue homeostasis. Binding of the secreted ligand to its Ptch1 receptor triggers Hh signaling through distinct canonical or noncanonical signaling pathways. Canonical Hh signaling leads to the activation of Gli transcription factors to induce Hh target...

journal_title：Biochemical and biophysical research communications

authors： Lee H,Ko HW

更新日期：2016-11-25 00:00:00

abstract：:Amphibian skin contains rich bradykinin-related peptides, but the mode of biosynthesis of these peptides is unknown. In the present study, a novel bradykinin-related peptide, termed bombinakinin M, was purified from skin secretions of the Chinese red belly toad Bombina maxima. Its primary sequence was established as D...

journal_title：Biochemical and biophysical research communications

authors： Lai R,Liu H,Hui Lee W,Zhang Y

更新日期：2001-08-17 00:00:00

abstract：:A novel protein kinase activity was characterized from the cytosolic fraction of isolated rabbit gastric glands. The kinase phosphorylated a major 33,000 Da endogenous protein (pp33) and was stimulated by Zn2+ and Mn2+ with Kact of 1.0 and 7.5 mM, respectively. Mg2+ and Ca2+ failed to stimulate any pp33 kinase activit...

journal_title：Biochemical and biophysical research communications

authors： Goldenring JR,Oddsdottir M,Modlin IM

更新日期：1987-01-30 00:00:00

abstract：:Rho family proteins are constitutively activated in the highly invasive human fibrosarcoma HT1080 cells. We now investigated the specific roles of Rac1 and Rac2 in regulating morphology, F-actin organization, adhesion, migration, and chemotaxis of HT1080 cells. Downregulation of Rac1 using specific siRNA probes result...

journal_title：Biochemical and biophysical research communications

authors： Niggli V,Schlicht D,Affentranger S

更新日期：2009-09-04 00:00:00

abstract：:Depending on culture in either "low Ca++" (0.25 mM) or "normal Ca++" (1.8 mM) medium, human colon adenocarcinoma-derived CaCo-2 cells exhibit differential sensitivity to the antiproliferative action of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) and of two side-chain modified analogs, 1,25S,26-trihydroxy-delta 22-vitamin D...

journal_title：Biochemical and biophysical research communications

authors： Cross HS,Huber C,Peterlik M

更新日期：1991-08-30 00:00:00

abstract：:Atomic force microscopy (AFM) allows to study the molecular structure of biological macromolecules with nm to A resolutions without crystallization. We show here the applicability of AFM in the quantitative analysis of the molecular mechanisms of DNA/protein interaction: (i) Protein-binding sites can be mapped over a ...

journal_title：Biochemical and biophysical research communications

authors： Nettikadan S,Tokumasu F,Takeyasu K

更新日期：1996-09-24 00:00:00

abstract：:HOX genes are transcription factors that play important roles in body patterning and many cellular processes during embryonic, fetal, and adult development. Given their important function in normal tissues, it is reasonable to assume that abnormal expression of HOX genes in adults could lead to serious diseases such a...

journal_title：Biochemical and biophysical research communications

authors： Lee JY,Kim JM,Jeong DS,Kim MH

更新日期：2018-09-18 00:00:00

abstract：AIM:To investigate the effect of endogenous PTH deficiency on osteoclasts during fracture healing and its mechanism. METHODS:A femoral fracture model was used to determine the role of endogenous PTH in fracture healing. Immunohistochemistry, qPCR, and Western blot were used to determine the potential functions and mec...

journal_title：Biochemical and biophysical research communications

authors： Sun P,Wang M,Yin GY

更新日期：2020-05-14 00:00:00

abstract：:It has been known that Porphyromonas gingivalis has an obligate requirement for hemin or selected heme- or Fe-containing compounds for its growth. In addition, the influence of hemin on the expression of several putative virulence factors produced by this bacterium has also been recently documented; however, the mecha...

journal_title：Biochemical and biophysical research communications

authors： Shibata Y,Hiratsuka K,Hayakawa M,Shiroza T,Takiguchi H,Nagatsuka Y,Abiko Y

更新日期：2003-01-10 00:00:00

abstract：:Glycogen synthase kinase (GSK)-3β interaction protein (GSKIP), a key regulator of signaling transduction, is implicated in multiple pathological processes. However, whether GSKIP is involved in myocardial infarction is unknown. The present study was designed to determine the potential involvement of GSKIP in myocardia...

journal_title：Biochemical and biophysical research communications

authors： Yan L,Cheng G,Yang G

更新日期：2020-10-29 00:00:00

abstract：:AK2 is an adenylate phosphotransferase that localizes at the intermembrane spaces of the mitochondria, and its mutations cause a severe combined immunodeficiency with neutrophil maturation arrest named reticular dysgenesis (RD). Although the dysfunction of hematopoietic stem cells (HSCs) has been implicated, earlier d...

journal_title：Biochemical and biophysical research communications

authors： Oshima K,Saiki N,Tanaka M,Imamura H,Niwa A,Tanimura A,Nagahashi A,Hirayama A,Okita K,Hotta A,Kitayama S,Osawa M,Kaneko S,Watanabe A,Asaka I,Fujibuchi W,Imai K,Yabe H,Kamachi Y,Hara J,Kojima S,Tomita M,Soga T,

更新日期：2018-03-04 00:00:00

abstract：:We have investigated the effects of staurosporine (STP), an inhibitor of PKC, on the expression of the HSP70 gene in human colon carcinoma HT-29 cells. Cells were heated at 45 degrees C for 15 min and incubated at 37 degrees C for up to 6 hr with or without STP. When STP (5 micrograms/ml) was added during 37 degrees C...

journal_title：Biochemical and biophysical research communications

authors： Erdos G,Lee YJ

更新日期：1994-07-15 00:00:00

abstract：:Little is known about the gene structure and mRNA expression of cholecystokinin type-A(CCKA) receptor in animals or in human. By screening an EMBL phage library containing partially digested Long-Evans rat DNA, we isolated 7 genomic clones covering a continuous 27 kb region over which a rat CCKA receptor gene spanned....

journal_title：Biochemical and biophysical research communications

authors： Takata Y,Takiguchi S,Funakoshi A,Kono A

更新日期：1995-08-24 00:00:00

abstract：:Bacteriocin LB44 was purified from cell-free supernatant (CFS) of Pediococcus pentosaceus LB44 using activity-guided chromatography techniques. It was stable up to 121 °C, pH 2.0-6.0, sensitive to proteinase K, papain and trypsin, and retained complete activity in the presence of organic solvents tested. The molecular...

journal_title：Biochemical and biophysical research communications

authors： Kaur R,Tiwari SK

更新日期：2018-04-15 00:00:00

abstract：:Protein kinase C appears to play an important, yet complex role in the supramaximal inhibition of pancreatic acinar cell secretion observed in response to cholecystokinin (CCK). The addition of protein kinase C activation to the concentration-response curve of a partial agonist acting at the CCK receptor (a phenethyl ...

journal_title：Biochemical and biophysical research communications

authors： Gaisano HY,Miller LJ

更新日期：1992-08-31 00:00:00

abstract：:The genomic sequence of the halophilic archaeon Halobacterium NRC-1 has been analyzed by the Z curve method. The Z curve is a three-dimensional curve that uniquely represents a given DNA sequence. Based on the known behaviors of the Z curves for the archaea whose replication origins have been identified, the analysis ...

journal_title：Biochemical and biophysical research communications

authors： Zhang R,Zhang CT

更新日期：2003-03-21 00:00:00

abstract：:Transforming growth factor-beta1 and fibroblast growth factor-2 play very important roles in fibroblast proliferation and collagen expression. These processes lead to the formation of joint adhesions through the SMAD and MAPK pathways, in which extracellular signal-regulated kinase (ERK)2 is considered to be crucial. ...

journal_title：Biochemical and biophysical research communications

authors： Li F,Ruan H,Fan C,Zeng B,Wang C,Wang X

更新日期：2010-01-01 00:00:00

abstract：:We found citrulline-containing proteins in mouse peritoneal macrophages undergoing calcium ionophore-induced apoptosis. Such proteins were products of deimination of arginine residues catalyzed by endogenous peptidylarginine deiminase (EC 3.5.3.15) activated by calcium influx. Western blotting analyses of the extract ...

journal_title：Biochemical and biophysical research communications

authors： Asaga H,Yamada M,Senshu T

更新日期：1998-02-24 00:00:00

abstract：:We used a lactose-substituted polystyrene, poly-N-p-vinylbenzyl-D-lactonamide (PVLA), as a substratum for adult rat hepatocytes in primary culture. Spherical-shaped hepatocytes attached on PVLA substratum formed stable multilayer aggregates anchored on substratum through the stimulation of epidermal growth factor (EGF...

journal_title：Biochemical and biophysical research communications

authors： Tobe S,Takei Y,Kobayashi K,Akaike T

更新日期：1992-04-15 00:00:00

abstract：:Recent evidence has revealed a novel signaling mechanism through which brown adipose tissue (BAT)-derived exosomal microRNAs (miRNAs) influence hepatic gene expression. Here, we uncover neuronal control of these miRNAs and identify exosomal miR-132-3p as a regulator of hepatic lipogenesis under cold stress conditions....

journal_title：Biochemical and biophysical research communications

authors： Kariba Y,Yoshizawa T,Sato Y,Tsuyama T,Araki E,Yamagata K

更新日期：2020-09-24 00:00:00

abstract：:Dysregulation of autophagy has been observed in obesity and type 2 diabetes. Salt-inducible kinase 2 (SIK2), a member of the AMPK-related kinase family, is downregulated in adipocytes from obese or insulin resistant individuals and was previously demonstrated to regulate autophagy in cancer and normal cell lines. The ...

journal_title：Biochemical and biophysical research communications

authors： Negoita F,Säll J,Morén B,Stenkula K,Göransson O

更新日期：2019-01-15 00:00:00

abstract：:Although antisense strategy has been at the center of interest in gene therapy, little is known about application of this strategy to cardiac diseases because of the lack of a suitable delivery method into the heart. Therefore, we compared the transfection efficiency of antisense oligodeoxynucleotides (ODN) using HVJ-...

journal_title：Biochemical and biophysical research communications

authors： Aoki M,Morishita R,Higaki J,Moriguchi A,Kida I,Hayashi S,Matsushita H,Kaneda Y,Ogihara T

更新日期：1997-02-24 00:00:00

abstract：:[3H] Nitrendipine binding was partially blocked by the presence of tetrodotoxin in developing spinal cord neurons. In young cultures, 1 micron tetrodotoxin displaced 29% and 26% of [3H] nitrendipine binding from the high and low affinity binding sites, respectively. In one month old cultures, tetrodotoxin had no effec...

journal_title：Biochemical and biophysical research communications

authors： Litzinger MJ,Brenneman DE

更新日期：1984-11-14 00:00:00

abstract：BACKGROUND AND AIMS:Acute graft-versus-host disease (GVHD) is a major complication after allogeneic hematopoietic stem cell transplantation, which often targets gastrointestinal (GI) tract. Osteopontin (OPN) plays an important physiological role in the efficient development of Th1 immune responses and cell survival by ...

journal_title：Biochemical and biophysical research communications

authors： Kawakami K,Minami N,Matsuura M,Iida T,Toyonaga T,Nagaishi K,Arimura Y,Fujimiya M,Uede T,Nakase H

更新日期：2017-04-01 00:00:00

abstract：:Protein phosphatase 2C (PP2C) is one of the four major protein serine/threonine phosphatases of eukaryotes and is implicated in the regulation of various cellular functions. With the goal of elucidating the mechanism responsible for regulating PP2C functions, we investigated the significance of phosphorylation of fiss...

journal_title：Biochemical and biophysical research communications

authors： Kobayashi T,Sadaie M,Ohnishi M,Wang H,Ikeda S,Hanada M,Yanagawa Y,Nakajima T,Tamura S

更新日期：1998-10-09 00:00:00

abstract：:Sorbitol levels in proximal tubule cells cultured for 96 h in the presence of 16.5 mM and 27.5 mM glucose were significantly elevated when compared to cells cultured in 5.5 mM glucose. No changes in the levels of aldose reductase activity, mRNA and immunoreactivity were observed in cells cultured for up to 168 h in me...

journal_title：Biochemical and biophysical research communications

authors： Petrash JM,Flath M,Sens D,Bylander J

更新日期：1992-08-31 00:00:00

abstract：:Human artificial chromosome (HAC) has several advantages as a gene therapy vector, including stable episomal maintenance and the ability to carry large gene inserts. Induced pluripotent stem (iPS) cells also have a great potential for gene therapy, which can be generated from an individual's own tissues and contribute...

journal_title：Biochemical and biophysical research communications

authors： Yakura Y,Ishihara C,Kurosaki H,Kazuki Y,Komatsu N,Okada Y,Doi T,Takeya H,Oshimura M

更新日期：2013-02-08 00:00:00

abstract：:Previous studies demonstrated that macrophages are difficult to transfect. In the present study, we investigated whether J774A.1 macrophages can be efficiently transfected using nucleofector technology. Nucleofection of J774A.1 macrophages with mRNA resulted in transfection efficiencies up to 75% without cell death as...

journal_title：Biochemical and biophysical research communications

authors： Van De Parre TJ,Martinet W,Schrijvers DM,Herman AG,De Meyer GR

更新日期：2005-02-04 00:00:00

abstract：:In vitro metabolism of the herbicide bentazon was studied in microsomal membranes isolated from 6-day-old etiolated corn shoots. Microsomes isolated from shoots of nontreated seeds did not metabolize bentazon when assayed with NADPH or peroxides. However, microsomes isolated from shoots of seeds pretreated with naphth...

journal_title：Biochemical and biophysical research communications

authors： McFadden JJ,Gronwald JW,Eberlein CV

更新日期：1990-04-16 00:00:00

abstract：:SIRT3 is a NAD(+)-dependent histone deacetylaseand and plays a critical role in various human carcinomas. However, its precise role in the pathogenesis of gastric cancer (GC) is still unclear. Western blot and Real-Time PCR were used to detect the protein and mRNA level of SIRT3 in freshly collected samples from GC pa...

journal_title：Biochemical and biophysical research communications

authors： Yang B,Fu X,Shao L,Ding Y,Zeng D

更新日期：2014-01-03 00:00:00