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Specific roles of Rac1 and Rac2 in motile functions of HT1080 fibrosarcoma cells.

Abstract：

:Rho family proteins are constitutively activated in the highly invasive human fibrosarcoma HT1080 cells. We now investigated the specific roles of Rac1 and Rac2 in regulating morphology, F-actin organization, adhesion, migration, and chemotaxis of HT1080 cells. Downregulation of Rac1 using specific siRNA probes resulted in cell rounding, markedly decreased spreading, adhesion, and chemotaxis of HT1080 cells. 2D migration on laminin-coated surfaces in contrast was not markedly affected. Selective Rac2 depletion did not affect cell morphology, cell adhesion, and 2D migration, but significantly reduced chemotaxis. Downregulation of both Rac1 and Rac2 resulted in an even more marked reduction, but not complete abolishment, of chemotaxis indicating distinct as well as overlapping roles of both proteins in chemotaxis. Rac1 thus is selectively required for HT1080 cell spreading and adhesion whereas Rac1 and Rac2 are both required for efficient chemotaxis.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Niggli V,Schlicht D,Affentranger S

doi

10.1016/j.bbrc.2009.06.098

subject

Has Abstract

pub_date

2009-09-04 00:00:00

pages

688-92

issue

4

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(09)01260-1

journal_volume

386

pub_type

杂志文章

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