Protein-binding DNA regions inside and in the vicinity of the cytochrome P450bm-3 gene from Bacillus megaterium.

abstract：:Tamoxifen, a widely used nonsteroidal antiestrogen in the treatment of breast cancer, forms several metabolites. 4-Hydroxytamoxifen (4-OHTam), a metabolite found in the bloodstream, has much higher affinity for the estrogen receptor than tamoxifen itself. Oxidative activation of 4-OHTam induces DNA damage. DNA isolate...

journal_title：Biochemical and biophysical research communications

authors： Sharma M

更新日期：2000-06-24 00:00:00

abstract：:E-selectin is a membrane protein expressed by endothelial cells activated by cytokines released during the inflammatory process; it plays an important role in neutrophil emigration into inflamed tissues. To further explore in vivo the function of E-selectin, we have generated transgenic mouse line expressing E-selecti...

journal_title：Biochemical and biophysical research communications

authors： Araki M,Araki K,Miyazaki Y,Iwamoto M,Izui S,Yamamura K,Vassalli P

更新日期：1996-07-25 00:00:00

abstract：:Interleukin-10 (IL-10) is a cytokine with many regulatory functions. In particular, IL-10 exerts neutralizing effect on other cytokines, and therefore IL-10 is thought to have important therapeutic implications. Recent reports suggest that IL-10 regulates not only immunocytes but also collagen and collagenase gene exp...

journal_title：Biochemical and biophysical research communications

authors： Yamamoto T,Eckes B,Krieg T

更新日期：2001-02-16 00:00:00

abstract：:The nucleotide sequence of the 5S rRNA from a mushroom, Coprinus cinereus, was determined to be: (sequence in text). This sequence is consistent with current models for the secondary structure of 5S RNAs and indicates a very high degree of sequence conservation among the most highly evolved fungi. Sequence heterogenei...

journal_title：Biochemical and biophysical research communications

authors： Liu WY,Nazar RN

更新日期：1983-10-14 00:00:00

abstract：:A polymerase chain reaction-generated mouse ACTH-R probe was used to screen a mouse genomic library, and a clone of 13kb containing the entire coding sequence was isolated. The coding sequence shows 84% homology with the human gene at the DNA level and encodes a peptide with 89% homology to the human ACTH-R. This gene...

journal_title：Biochemical and biophysical research communications

authors： Cammas FM,Kapas S,Barker S,Clark AJ

更新日期：1995-07-26 00:00:00

abstract：:Chromatography of a DNA polymerase preparation from mitochondria of Saccharomyces cerevisiae on DNA-cellulose column, using Tris-HCl (pH 7.5) buffer containing 0.6 M NaCl as eluent, was found to yield a fraction exhibiting DNA primase-like activity free of DNA polymerase. This fraction could support the synthesis of 1...

journal_title：Biochemical and biophysical research communications

authors： Desai SD,Pasupathy K,Chetty KG,Pradhan DS

更新日期：1989-04-28 00:00:00

abstract：:Pain is a common symptom of injuries and inflammatory-related conditions. The perception of pain, commonly known as nociception, depends on integrated receptors and molecular pathways. Inflammatory mediators are involved in the genesis, persistence, and severity of pain. Noxious stimuli can trigger a cascade of inflam...

journal_title：Biochemical and biophysical research communications

authors： Haddad JJ

更新日期：2007-02-09 00:00:00

abstract：:Neutrophils stimulated with a low concentration of chemotactic peptide N-formyl-met-leu-phe respond with a brief pulse of actin polymerization and a persistent change in morphology from round to polarized. The recovery of the actin polymerization response is unlikely to be due to usual receptor desensitization mechani...

journal_title：Biochemical and biophysical research communications

authors： Model MA,Omann GM

更新日期：1996-07-16 00:00:00

abstract：:Four peptides possessing both natriuretic activity and smooth muscle relaxant activity were isolated from rat atrium and their amino acid sequences determined. The four peptides designated ANF-I, ANF-II, ANF-III and ANF-IV containing 35, 31, 30 and 25 amino acid residues, respectively, were obtained in a molar ratio o...

journal_title：Biochemical and biophysical research communications

authors： Misono KS,Grammer RT,Fukumi H,Inagami T

更新日期：1984-09-17 00:00:00

abstract：:The muscarinic acetylcholine receptor was solubilized, in a sensitive form for GTP and Na+, from bovine cerebral cortex using a zwitterionic detergent 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane sulfonate. The solubilized muscarinic receptor displayed characteristics as follows: (1) high affinity to nanomolar co...

journal_title：Biochemical and biophysical research communications

authors： Kuno T,Shirakawa O,Tanaka C

更新日期：1983-05-16 00:00:00

abstract：:The expansion of a CAG trinucleotide repeat (TNR) sequence has been linked to several neurological disorders, for example, Huntington's disease (HD). In HD, healthy individuals have 5-35 CAG repeats. Those with 36-39 repeats have the premutation allele, which is known to be prone to expansion. In the disease state, gr...

journal_title：Biochemical and biophysical research communications

authors： Jarem DA,Delaney S

更新日期：2011-12-09 00:00:00

abstract：:To identify genes responding to the cholesterol-rich diet, differentially expressed hepatic genes have been searched from a diet-induced hypercholesterolemic rabbit by differential display reverse transcription-polymerase chain reaction (DDRT-PCR). Among the many screened genes, Rab7 gene was shown to be distinctively...

journal_title：Biochemical and biophysical research communications

authors： Kim JY,Jang MK,Lee SS,Choi MS,Bok SH,Oh GT,Park YB

更新日期：2002-04-26 00:00:00

abstract：:Reversible phosphorylation of RNA polymerase (RNAP) II's largest subunit C-terminal domain (CTD) is a key event during mRNA metabolism. The CTD phosphatase, FCP1, catalyzes the dephosphorylation of RNAP II and is thought to play a major role in polymerase recycling. In this study, we isolated a novel phosphatase gene ...

journal_title：Biochemical and biophysical research communications

authors： Zheng H,Ji C,Gu S,Shi B,Wang J,Xie Y,Mao Y

更新日期：2005-06-17 00:00:00

abstract：:Identifying Mycobacterium tuberculosis membrane proteins involved in binding to and invasion of host cells is important in designing subunit-based anti-tuberculosis vaccines. The Rv2969c gene sequence was identified by PCR in M. tuberculosis complex strains, being transcribed in M. tuberculosis H37Rv, M. tuberculosis ...

journal_title：Biochemical and biophysical research communications

authors： Patarroyo MA,Plaza DF,Ocampo M,Curtidor H,Forero M,Rodriguez LE,Patarroyo ME

更新日期：2008-08-08 00:00:00

abstract：:This study presents a kinetic analysis of the response to wounding in rice plants. In particular, jasmonic acid, salicylic acid, and lipoxygenase activity were measured in leaves of wounded rice plants during the early tillering phase. The results show that endogenous jasmonic acid transiently increases to a maximum 3...

journal_title：Biochemical and biophysical research communications

authors： Lee A,Cho K,Jang S,Rakwal R,Iwahashi H,Agrawal GK,Shim J,Han O

更新日期：2004-06-04 00:00:00

abstract：:The genetic encoding of synthetic or "non-natural" amino acids promises to diversify the functions and structures of proteins. We applied rapid codon-reassignment for creating Escherichia coli strains unable to terminate translation at the UAG "stop" triplet, but efficiently decoding it as various tyrosine and lysine ...

journal_title：Biochemical and biophysical research communications

authors： Mukai T,Yanagisawa T,Ohtake K,Wakamori M,Adachi J,Hino N,Sato A,Kobayashi T,Hayashi A,Shirouzu M,Umehara T,Yokoyama S,Sakamoto K

更新日期：2011-08-12 00:00:00

abstract：:Septic shock induced by lipopolysaccharide (LPS) is characterized by serious systemic inflammatory response and robust production of pro-inflammatory cytokines from activated macrophages. Damage-associated molecular patterns (DAMPs) secreted by activated macrophages are key contributors to septic shock. However, the c...

journal_title：Biochemical and biophysical research communications

authors： He Y,Peng Y,Tao L,Peng Z,Yang H

更新日期：2020-07-05 00:00:00

abstract：:Nasopharyngeal carcinoma (NPC) is a cancer arising from the nasopharynx epithelium. Long non-coding RNAs (lnc RNA) play a critical role in various biological processes such as cell growth, embryonic development, and tumorigenesis. In the study, for the first time, we discovered that lnc RNA taurine upregulated gene 1 ...

journal_title：Biochemical and biophysical research communications

authors： Qian W,Ren Z,Lu X

更新日期：2019-01-29 00:00:00

abstract：:Electrophoretic mobility shift assays revealed minimal levels of NF-kappaB activity in rat distal lung epithelial cells cultured at fetal (23 mmHg) or adult alveolar (100 mmHg) P(O2), but revealed significant activation of this transcription factor in cells exposed to a rise in P(O2) mimicking that experienced at birt...

journal_title：Biochemical and biophysical research communications

authors： Haddad JJ,Collett A,Land SC,Olver RE,Wilson SM

更新日期：2001-03-09 00:00:00

abstract：:The isolation and characterization of cofactor-independent phosphoglycerate mutase gene from maize (Zea mays) is here reported. This gene sequence constitutes the first described from this enzyme. The gene spans 5.4 kilo base pairs and has nine exons in the translated region. Canonical TATA and CCAAT and Sp1-like boxe...

journal_title：Biochemical and biophysical research communications

authors： Pérez de la Ossa P,Graña X,Ruiz-Lozano P,Climent F

更新日期：1994-09-15 00:00:00

abstract：:Ubiquitin (Ub) carboxyl-terminal hydrolase L1 (UCH-L1) has dual functions, such as hydrolase activity on the chemical bonds formed by the C-terminal Gly of Ub and dimerization-dependent ubiquitin ligase activity. Accumulating evidence suggests that dual activities of UCH-L1 were intimately associated with Parkinson's ...

journal_title：Biochemical and biophysical research communications

authors： Park DW,Nam MK,Rhim H

更新日期：2011-11-11 00:00:00

abstract：:In this study, we used, for the first time, atomic force microscope (AFM) images to investigate the mode of action of DNA topoisomerase I (topo I) in the presence and absence of its inhibitors: camptothecin (CPT) and tyrphostin AG-1387. The results revealed that in the absence of the inhibitors, the enzyme relaxed sup...

journal_title：Biochemical and biophysical research communications

authors： Argaman M,Bendetz-Nezer S,Matlis S,Segal S,Priel E

更新日期：2003-02-14 00:00:00

abstract：:Meiotic cells of Coprinus contain a protein that can be identified by its ability to enhance activity of the meiotic DNA polymerase reported previously (8). Its activity is found only during the prophase stages in meiotic cells, and is accompanied by the meiotic polymerase. The protein, which was purified to near homo...

journal_title：Biochemical and biophysical research communications

authors： Gomi K,Sakaguchi K

更新日期：1994-02-15 00:00:00

abstract：:Therapeutic effects of combination of angiogenic growth factors for the treatment of ischemia after myocardial infarction are largely unknown. Plasmids expressing basic fibroblast growth factor (bFGF), platelet-derived growth factor (PDGF-BB) or their combination with a 1:1 mass ratio were injected into hearts with 7-...

journal_title：Biochemical and biophysical research communications

authors： Hao X,Månsson-Broberg A,Gustafsson T,Grinnemo KH,Blomberg P,Siddiqui AJ,Wärdell E,Sylvén C

更新日期：2004-03-19 00:00:00

abstract：:Bacillus alcalophilus, an obligately alkalophilic bacterium that grows at pH 11.0, has an intracellular pH of 9.5 or less. Unlike all other living organisms, polyamines (putrescine, spermidine and spermine) in B. alcalophilus, if present, will be largely unprotonated. HPLC analysis indicated that spermidine is the maj...

journal_title：Biochemical and biophysical research communications

authors： Chen KY,Cheng S

更新日期：1988-01-15 00:00:00

abstract：:L-amino acids represent the most common amino acid form, most notably as protein residues, whereas D-amino acids, despite their rare occurrence, play significant roles in many biological processes. Amino acid racemases are enzymes that catalyze the interconversion of L- and/or D-amino acids. McyF is a pyridoxal 5'-pho...

journal_title：Biochemical and biophysical research communications

authors： Cao DD,Zhang CP,Zhou K,Jiang YL,Tan XF,Xie J,Ren YM,Chen Y,Zhou CZ,Hou WT

更新日期：2019-07-05 00:00:00

abstract：:The 5mC DNA methyltransferase M.HhaI can be split into two individually inactive N- and C-terminal fragments that together can form an active enzyme in vivo capable of efficiently methylating DNA. This active fragment pair was identified by creating libraries of M.HhaI gene fragment pairs and then selecting for the pa...

journal_title：Biochemical and biophysical research communications

authors： Choe W,Chandrasegaran S,Ostermeier M

更新日期：2005-09-09 00:00:00

abstract：:Ornithine decarboxylase (ODC) was induced in the liver, lung and brain of the mouse injected intraperitoneally with 12-O-tetradecanoylphorbol 13-acetate (TPA), showing maximal enzyme activity four hours after the injection. The increase of ODC activity was due to the enhanced syntheses of mRNA and protein. The inducti...

journal_title：Biochemical and biophysical research communications

authors： Hibasami H,Tsukada T,Maekawa S,Sakurai M,Nakashima K

更新日期：1987-04-29 00:00:00

abstract：:Serum-free medium from batch cultures of Sf9 insect cells was examined for the occurrence of proteins related to the insulin-like growth factor family. We found that the Sf9 cell line constitutively produced and secreted a soluble protein with a MW of 27 kDa that exerted specific binding to human insulin-like growth f...

journal_title：Biochemical and biophysical research communications

authors： Doverskog M,Tally M,Häggström L

更新日期：1999-11-30 00:00:00

abstract：:Contraction of rat uterine smooth muscle related to phosphorylation state of myosin light chain under various conditions was investigated. In the Ca2(+)-containing medium, both high K+ and oxytocin induced marked contraction of the muscle accompanied by pronounced phosphorylation of myosin light chain. In the Ca2(+)-f...

journal_title：Biochemical and biophysical research communications

authors： Oishi K,Takano-Ohmuro H,Minakawa-Matsuo N,Suga O,Karibe H,Kohama K,Uchida MK

更新日期：1991-04-15 00:00:00