Human B lymphocytes possess 5-lipoxygenase activity and convert arachidonic acid to leukotriene B4.

Abstract:

:Incubation of cell sonicates from monoclonal B cells with arachidonic acid led to the formation of leukotriene (LT) B4 and 5-hydroxy-eicosatetraenoic acid (5-HETE). In contrast, stimulation of intact B cells with the calcium ionophore A23187 +/- arachidonic acid did not, under similar conditions, lead to formation of LTB4. The identification of these products was based on reverse phase- and straight phase-HPLC analysis, UV-spectroscopy and gas chromatography-mass spectrometry. Cell sonicates of highly enriched human tonsillar B lymphocytes also converted arachidonic acid to LTB4 and 5-HETE. Activation of these cells with B cell mitogen and cytokines for three days led to an upregulation of 5-lipoxygenase activity. This study provides evidence for the biosynthesis of LTB4 from arachidonic acid in B cell lines and in normal human tonsillar B lymphocytes.

authors

Jakobsson PJ,Odlander B,Steinhilber D,Rosén A,Claesson HE

doi

10.1016/0006-291x(91)91814-s

subject

Has Abstract

pub_date

1991-07-15 00:00:00

pages

302-8

issue

1

eissn

0006-291X

issn

1090-2104

pii

0006-291X(91)91814-S

journal_volume

178

pub_type

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