当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Transcriptional regulation of mouse MARCKS promoter in immortalized hippocampal cells.

Transcriptional regulation of mouse MARCKS promoter in immortalized hippocampal cells.

Abstract：

:Mouse MARCKS is a prominent myristoylated alanine-rich C kinase substrate implicated in brain development, calcium/calmodulin signaling, and membrane cytoskeletal restructuring, and is developmentally regulated in a cell- and tissue-specific fashion. In this study, transcriptional regulation of mouse MARCKS promoter in the neuronally derived immortalized hippocampal cells (HN33) was examined for a portion of 5'-flanking genomic sequence from -993 to +1 relative to the translation start site. Transfection experiments carried out in this neural cell line identified, for the first time, that the distal promoter segment from -993 to -713 plays a crucial role as an enhancer/activator element in the up-regulation of the basal transcription activity driven by MARCKS core promoter sequence. Motif analyses revealed at least 12 overlapping potential transcription factor binding sites in this region, among which a prominent GA-rich sequence centered at -765 has been shown to be functionally important in the binding of Sp1 protein-like complex. Deletion of the GA-rich segment significantly reduced the MARCKS promoter activity. Further, competitive EMSA indicated two additional sites within the -993/-713 that may also interact with Sp1 protein, demonstrating that the activator function of -993/-713 is under control of multiple Sp1 transcription factors. Unlike the distal promoter sequence, the proximal core promoter sequence (-649/-438) contains a GC-rich box and a Z-DNA-forming segment and is critical to basal transcription. The deletion of -649/-438 segment has been shown to drastically impair the promoter activity even in the presence of -993/-713, suggesting that its presence is also important to the function of -993/-713. These data emphasize that the synergistic interaction between distal and proximal promoter sequences is indispensable for the optimal MARCKS promoter function in the immortalized hippocampal cells. The discovery of the activator function of the MARCKS distal promoter region, and its potential interaction with multiple Sp proteins may provide a new clue to the understanding of Macs transcriptional regulation in brain.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Wang L,Liu X,Lenox RH

doi

10.1006/bbrc.2002.6655

subject

Has Abstract

pub_date

2002-04-12 00:00:00

pages

969-79

issue

4

eissn

0006-291X

issn

1090-2104

pii

S0006291X02966556

journal_volume

292

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Mitochondrial malate dehydrogenase and its precursor have different conformations.

    abstract：:Antiserum prepared against the denatured form of mammalian malate dehydrogenase was found to immunoprecipitate the denatured but not the native form of the mature enzyme. In contrast, the antiserum immunoprecipitated the enzyme's precursor, synthesized in a rabbit reticulocyte lysate, either before or after denaturati...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(86)80370-9

    authors： Chien SM,Freeman KB

    更新日期：1986-11-26 00:00:00

  • The effect of variable domain orientation and arrangement on the antigen-binding activity of a recombinant human bispecific diabody.

    abstract：:In recent years a variety of recombinant methods have been developed for efficient production of bispecific antibodies (BsAb) in various formats. Bispecific diabody (bDAb), a 55-60 kDa molecule comprising two non-covalently associated cross-over single chain Fv (scFv) polypeptides, represents one of the most promising...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.04.060

    authors： Lu D,Jimenez X,Witte L,Zhu Z

    更新日期：2004-05-28 00:00:00

  • Comparison of mucosal absorption-enhancing activity between a claudin-3/-4 binder and a broadly specific claudin binder.

    abstract：:Intercellular spaces between adjacent mucosal epithelial cells are sealed by tight junctions (TJs) that prevent the free movement of solutes across the epithelium. Claudins (CLs), a family of 27 integral membrane proteins, are essential components for TJ seals. We previously used a CL-3/-4 binder, the C-terminal fragm...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.05.060

    authors： Matsuhisa K,Kondoh M,Suzuki H,Yagi K

    更新日期：2012-06-29 00:00:00

  • EGCG inhibits activation of the insulin-like growth factor-1 receptor in human colon cancer cells.

    abstract：:The IGF/IGF-1R system, which includes the IGF, IGF-1R, and IGFBPs proteins, plays an important role in the development and growth of colorectal cancer. We previously reported that in the HT29 human colon cancer cell line EGCG, the major biologically active component of green tea, inhibits activation of the RTKs EGFR, ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.06.182

    authors： Shimizu M,Deguchi A,Hara Y,Moriwaki H,Weinstein IB

    更新日期：2005-09-02 00:00:00

  • Phospholipase C: a putative mechanotransducer for endothelial cell response to acute hemodynamic changes.

    abstract：:Endothelial cells (EC) in vivo are exposed to a multitude of physical forces with each pulse of the cardiac cycle. Ongoing studies support the concept that EC respond to these forces through specific signal transduction pathways. Previous investigations in our laboratory have shown that EC respond to the initiation of...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.1087

    authors： Brophy CM,Mills I,Rosales O,Isales C,Sumpio BE

    更新日期：1993-01-29 00:00:00

  • Structure of acrosome reaction-inducing substance in the jelly coat of starfish eggs: a mini review.

    abstract：:Our knowledge at present on the structure of acrosome-reaction inducing substance (ARIS) in the jelly coat of starfish eggs is summarized. ARIS ia a proteoglycan-like molecule consisting of very long, linear, and highly sulfated glycans and three ARIS proteins, ARIS1-3. Detailed structures of the major glycan of ARIS ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章,评审

    doi：10.1016/j.bbrc.2012.08.033

    authors： Hoshi M,Moriyama H,Matsumoto M

    更新日期：2012-08-31 00:00:00

  • Calcitonin gene-related peptide(CGRP) stimulates the release of atrial natriuretic peptide(ANP) from isolated rat atria.

    abstract：:The effect of calcitonin gene-related peptide(CGRP) on the release of atrial natriuretic peptide(ANP) was studied in spontaneously beating, isolated rat atria. CGRP stimulated the ANP release in a dose-dependent manner. When the atria were incubated with a combination of phentolamine, propranolol, and atropine, these ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(88)81304-4

    authors： Yamamoto A,Kimura S,Hasui K,Fujisawa Y,Tamaki T,Fukui K,Iwao H,Abe Y

    更新日期：1988-09-30 00:00:00

  • Effect of triiodothyronine on muscle cell differentiation and blood glucose level in hyperglycemic KK mice.

    abstract：:KK mice are genetically diabetic animals, showing glucose intolerance and insulin resistance. We examined the effects of 3,3',5-triiodo-L-thyronine (T3) on the blood glucose level and on mRNA levels of muscle cell differentiation markers in hyperglycemic KK mice. T3 treatment (T1, 1 mg; T3, 3 mg; T10, 10 mg/kg/day) of...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.6882

    authors： Shimokawa T,Kato M,Shioduka K,Irie J,Ezaki O

    更新日期：1997-06-27 00:00:00

  • Evidence that two reports of mtDNA cytochrome c oxidase "mutations" in Alzheimer's disease are based on nDNA pseudogenes of recent evolutionary origin.

    abstract：:Recently, two reports [R. E. Davis et al. (1997) Proc. Natl. Acad. Sci. USA 94, 4564-4569 and E. Fahy et al. (1997) Nucleic Acids Res. 25, 3102-3109] described a series of heteroplasmic mitochondrial DNA (mtDNA) mutations in the genes encoding two cytochrome c oxidase subunits (CO1 and CO2) which segregated in higher ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.8353

    authors： Davis JN 2nd,Parker WD Jr

    更新日期：1998-03-27 00:00:00

  • NC1 domain of human type VIII collagen (alpha 1) inhibits bovine aortic endothelial cell proliferation and causes cell apoptosis.

    abstract：:Endostatin, a natural angiogenesis inhibitor, had been identified for years. It opened a new approach for cancer therapy. Sequence analysis revealed that endostatin is the NC1 domain (non-triple-helical domain) of collagen XVIII. In this report, the cDNA of NC1 domain of type VIII collagen (alpha 1) was cloned and exp...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.5970

    authors： Xu R,Yao ZY,Xin L,Zhang Q,Li TP,Gan RB

    更新日期：2001-11-23 00:00:00

  • Human myeloma cells acquire resistance to difluoromethylornithine without overproducing ornithine decarboxylase.

    abstract：:An exposure of a human myeloma cell line to 2-difluoromethylornithine the mechanism-based inhibitor of ornithine decarboxylase (EC 4.1.1.17), resulted in a selection of tumor cells readily growing in the presence of 4 mM difluoromethylornithine, a concentration that swiftly halted the growth of the parental cells. Det...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(87)80485-0

    authors： Alhonen-Hongisto L,Leinonen P,Laine R,Jänne J

    更新日期：1987-04-14 00:00:00

  • Silencing Nrf2 impairs glioma cell proliferation via AMPK-activated mTOR inhibition.

    abstract：:Gliomas are the leading cause of death among adults with primary brain malignancies. Treatment for malignant gliomas remains limited, and targeted therapies have been incompletely explored. Nuclear factor erythroid 2-related factor 2 (Nrf2), a key transcription regulator for antioxidant and detoxification enzymes, is ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.12.034

    authors： Jia Y,Wang H,Wang Q,Ding H,Wu H,Pan H

    更新日期：2016-01-15 00:00:00

  • Specific binding of plasminogen to vitronectin. Evidence for a modulatory role of vitronectin on fibrin(ogen)-induced plasmin formation by tissue plasminogen activator.

    abstract：:Vitronectin immobilized onto polystyrene microtiter wells was demonstrated to specifically bind plasminogen in a concentration-dependent manner, yielding an estimated KD = 0.4 microM. Heparin only moderately interfered with the vitronectin-plasminogen interaction, whereas high concentrations of 6-amino-hexanoic acid i...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)91123-a

    authors： Preissner KT

    更新日期：1990-05-16 00:00:00

  • Peripheral blood monocytes from the decompensated liver cirrhosis could migrate into nude mouse liver with human hepatocyte-markers expression.

    abstract：:Adult stem cells provide a promising alternative for the treatment of decompensated liver cirrhosis. Our previous study showed that peripheral blood monocytes (PBMCs) from decompensated liver cirrhosis could differentiate into hepatocyte-like cells in vitro. We here aimed to investigate the differential potential of t...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.04.058

    authors： Yan L,Han Y,Wang J,Liu J,He Y,Wang H,Fan D

    更新日期：2008-07-11 00:00:00

  • Tumor promotion by exposure to radiofrequency electromagnetic fields below exposure limits for humans.

    abstract：:The vast majority of in vitro and in vivo studies did not find cancerogenic effects of exposure to electromagnetic fields (RF-EMF), i.e. emitted by mobile phones and base stations. Previously published results from a pilot study with carcinogen-treated mice, however, suggested tumor-promoting effects of RF-EMF (Tillma...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.02.151

    authors： Lerchl A,Klose M,Grote K,Wilhelm AF,Spathmann O,Fiedler T,Streckert J,Hansen V,Clemens M

    更新日期：2015-04-17 00:00:00

  • Generation of neutralizing monoclonal antibodies against Enterovirus 71 using synthetic peptides.

    abstract：:Enterovirus 71 (EV71) has led to recent outbreaks of hand, foot and mouth disease (HFMD) in China, resulting in high mortality. In this study, several monoclonal antibodies were generated by immunizing mice with two synthetic peptides, SP55 and SP70, containing amino acids 163-177 and 208-222 of VP1. The specificities...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.09.103

    authors： Li X,Mao C,Ma S,Wang X,Sun Z,Yi Y,Guo M,Shen X,Sun L,Bi S

    更新日期：2009-12-25 00:00:00

  • Calreticulin-integrin bidirectional signaling complex.

    abstract：:Calreticulin has multiple functions, diverse cellular locations, and putative isoforms. It likely maintains integrin avidity by binding alpha integrin cytoplasmic tails and is a surface lectin which triggers cell spreading. In the present study, we have immunocaptured a cell surface complex from B16 mouse melanoma cel...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.6195

    authors： Zhu Q,Zelinka P,White T,Tanzer ML

    更新日期：1997-03-17 00:00:00

  • Copper ions induce dityrosine-linked dimers in human but not in murine islet amyloid polypeptide (IAPP/amylin).

    abstract：:Dysregulation and aggregation of the peptide hormone IAPP (islet amyloid polypeptide, a.k.a. amylin) into soluble oligomers that appear to be cell-toxic is a known aspect of diabetes mellitus (DM) Type 2 pathology. IAPP aggregation is influenced by several factors including interactions with metal ions such as Cu(II)....

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.01.120

    authors： Dong X,Svantesson T,Sholts SB,Wallin C,Jarvet J,Gräslund A,Wärmländer SKTS

    更新日期：2019-03-19 00:00:00

  • Role of c-Src and focal adhesion kinase in progression and metastasis of estrogen receptor-positive breast cancer.

    abstract：:The non-receptor tyrosine kinases c-Src and focal adhesion kinase (Fak) mediate signal transduction pathways that regulate cell proliferation, survival, invasion, and metastasis. Here, we investigated whether c-Src and Fak are activated during progression of hormone-dependent breast cancer. Maximally active c-Src was ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.12.164

    authors： Planas-Silva MD,Bruggeman RD,Grenko RT,Stanley Smith J

    更新日期：2006-03-03 00:00:00

  • Evidence for a regulatory action of vanadate on protein phosphorylation in brain microvessels.

    abstract：:We investigated the action of vanadate on protein phosphorylation in microvessels isolated from rat brain. We found that a stimulation of protein phosphorylation from 32P-ATP occurs, in the presence of different concentrations of vanadate, 10(-3) M being the most effective dose. This action was time-dependent, and it ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)92289-4

    authors： Catalán RE,Martínez AM,Aragonés MD,Díaz G

    更新日期：1989-09-15 00:00:00

  • Quantitative assessment of cellular uptake and cytosolic access of antibody in living cells by an enhanced split GFP complementation assay.

    abstract：:Considering the number of cytosolic proteins associated with many diseases, development of cytosol-penetrating molecules from outside of living cells is highly in demand. To gain access to the cytosol after cellular uptake, cell-penetrating molecules should be released from intermediate endosomes prior to the lysosoma...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.10.066

    authors： Kim JS,Choi DK,Park SW,Shin SM,Bae J,Kim DM,Yoo TH,Kim YS

    更新日期：2015-11-27 00:00:00

  • β-cell serotonin production is associated with female sex, old age, and diabetes-free condition.

    abstract：:Serotonin is known to be present in pancreatic β-cells and to play several physiological roles, including insulin secretion, β-cell proliferation, and paracrine inhibition of α-cells. However, the serotonin production of different cell lines and islets has not been compared based on age, sex, and diabetes related cond...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.09.130

    authors： Kim YG,Moon JH,Kim K,Kim H,Kim J,Jeong JS,Lee J,Kang S,Park JS,Kim H

    更新日期：2017-11-25 00:00:00

  • Single cell analysis of changes in cytosolic calcium induced by vitamin D3 metabolites in cultured rat mesangial cells.

    abstract：:The acute effects of 1,25-Dihydroxy-vitamin D3 [1,25(OH)2D3] on the concentration of cytoplasmic ionized calcium [Ca2+] of cultured rat mesangial cells were studied at the single cell level by microspectrofluorometry of fura-2-loaded cells. Addition of 1,25(OH)2D3 produced an immediate increase of [Ca2]+. This rise in...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)81140-4

    authors： Boland RL,de Boland AR,Nobiling R,Weinreich T,Ritz E

    更新日期：1991-10-31 00:00:00

  • Cloning and sequencing of human FSH receptor cDNA.

    abstract：:We have isolated and sequenced a cDNA encoding the follicle stimulating hormone (FSH) receptor. The deduced amino acid sequence (678 residues) containing seven putative transmembrane segments which displays sequence similarity to G protein-coupled receptors. The receptor consists of 359 residue extracellular domain wh...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)91682-3

    authors： Minegishi T,Nakamura K,Takakura Y,Ibuki Y,Igarashi M,Minegish T corrected to Minegishi T

    更新日期：1991-03-29 00:00:00

  • Histamine-induced phosphoinositide metabolism in cultured human umbilical vein endothelial cells. Association with thromboxane and prostacyclin release.

    abstract：:Histamine stimulation of cultured human umbilical vein endothelial cells induced dose- and time-dependent increases in glycerophosphoinositol (GroPIns), inositol-1-phosphate (InsP), inositolbisphosphate (InsP2) and inositoltrisphosphate (InsP3) in addition to release of thromboxane A2 and prostacyclin. Increases in In...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(87)80529-6

    authors： Resink TJ,Grigorian GYu,Moldabaeva AK,Danilov SM,Bühler FR

    更新日期：1987-04-14 00:00:00

  • A recombinant adenoviral vector encoding functional vasoactive intestinal peptide.

    abstract：:Vasoactive intestinal peptide (VIP) is a small neuropeptide, which exerts pleiotropic functions. Based on its immunomodulatory, secretory, and possibly trophic effects, VIP is a valuable candidate molecule for the management of autoimmune disease. The purpose of this study was to develop a recombinant viral vector cap...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.04.171

    authors： Lodde BM,Delporte C,Goldsmith CM,Tak PP,Baum BJ

    更新日期：2004-06-18 00:00:00

  • Dynamics of the EAG1 K+ channel selectivity filter assessed by molecular dynamics simulations.

    abstract：:EAG1 channels belong to the KCNH family of voltage gated potassium channels. They are expressed in several brain regions and increased expression is linked to certain cancer types. Recent cryo-EM structure determination finally revealed the structure of these channels in atomic detail, allowing computational investiga...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.01.064

    authors： Bernsteiner H,Bründl M,Stary-Weinzinger A

    更新日期：2017-02-26 00:00:00

  • Rat kidney microsomes convert 25-hydroxyvitamin D3 to an unidentified metabolite.

    abstract：:Kidney microsomes from vitamin D-deficient rats and from thyroparathyroidectomized rats converted 25-hydroxyvitamin D3 to an unidentified metabolite. The addition of the cytosolic fraction enhanced microsomal synthesis of this metabolite two-fold. The kinetics of the conversion in the presence of the cytosolic fractio...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(83)91027-6

    authors： Thierry-Palmer M,Gray TK,Williams ME,Talmage RV

    更新日期：1983-02-10 00:00:00

  • Ca2+-overload inhibits the cardiac SR Ca2+-calmodulin protein kinase activity.

    abstract：:There is increasing evidence to suggest that Ca2+-calmodulin dependent protein kinase (CaMK) regulates the sarcoplasmic reticulum (SR) function and thus plays an important role in modulating the cardiac performance. Because intracellular Ca2+-overload is an important factor underlying cardiac dysfunction in a heart di...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/S0006-291X(02)00287-5

    authors： Netticadan T,Temsah RM,Kawabata K,Dhalla NS

    更新日期：2002-05-03 00:00:00

  • Inhibition of ADP-ribosyltransferase activity of cholera toxin by MDL 12330A and chlorpromazine.

    abstract：:ADP-ribosylation by cholera toxin of the guanine nucleotide binding regulatory protein (Gs) of rat liver membrane adenylate cyclase was inhibited by 0.1-1 mM MDL 12330A or 0.1-1 mM chlorpromazine. Basal as well as cholera toxin activated adenylate cyclase activity in liver membranes was also inhibited by the two drugs...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(84)91312-3

    authors： Bitonti AJ

    更新日期：1984-04-30 00:00:00