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Generation of neutralizing monoclonal antibodies against Enterovirus 71 using synthetic peptides.

Abstract：

:Enterovirus 71 (EV71) has led to recent outbreaks of hand, foot and mouth disease (HFMD) in China, resulting in high mortality. In this study, several monoclonal antibodies were generated by immunizing mice with two synthetic peptides, SP55 and SP70, containing amino acids 163-177 and 208-222 of VP1. The specificities of the anti-EV71 peptide monoclonal antibodies were confirmed by Western blot analysis and immunocytochemistry against EV71 virus. Most importantly, we have identified a monoclonal antibody, clone 22A12, which shows strong neutralizing activity against EV71 in an in vitro neutralization assay. Because there is no vaccine available and treatment is very limited, mouse anti-EV71 monoclonal antibody, clone 22A12, could be a promising candidate to be humanized and used for treatment of EV71 infection.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Li X,Mao C,Ma S,Wang X,Sun Z,Yi Y,Guo M,Shen X,Sun L,Bi S

doi

10.1016/j.bbrc.2009.09.103

subject

Has Abstract

pub_date

2009-12-25 00:00:00

pages

1126-8

issue

4

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(09)01920-2

journal_volume

390

pub_type

杂志文章

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