Phospholipase C: a putative mechanotransducer for endothelial cell response to acute hemodynamic changes.

abstract：:Comparative fluorimetric studies on the long-term (8-hour) action of beta[1-42]amyloid and its shorter fragments beta[1-40], beta[25-35] and beta[31-35] on the steady-state intracellular Ca2+ concentration in primary cultures of rat astroglial cells using the Ca2+-sensitive fluorescent probe Fura-2 AM revealed higher ...

journal_title：Biochemical and biophysical research communications

authors： Laskay G,Zarándi M,Varga J,Jost K,Fónagy A,Torday C,Latzkovits L,Penke B

更新日期：1997-06-27 00:00:00

abstract：:Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) may play important roles during hepatitis B virus (HBV) infection. In this study, we used a recombinant human soluble death receptor 5 (sDR5) to explore its effect in a mouse model of HBV-induced acute hepatitis. By measuring blood transaminase activity a...

journal_title：Biochemical and biophysical research communications

authors： Liu YG,Liu SX,Liang XH,Zhang Q,Gao LF,Han LH,Cao YL,Hou N,Du J,Sun WS

更新日期：2007-01-12 00:00:00

abstract：:RNF8 is a nuclear protein having an N-terminal forkhead-associated (FHA) domain and a C-terminal RING-finger (RF) domain. Depletion of RNF8 caused cell growth inhibition and cell cycle arrest at not only S but also G2/M phases. In addition, cell death was frequently observed in RNF8-depleted cells. Analyses of time-la...

journal_title：Biochemical and biophysical research communications

authors： Yoshioka T,Kimura M,Saio M,Era S,Okano Y

更新日期：2011-06-24 00:00:00

abstract：:Bovine embryonic stem-like cells (ES-like cells) appear to maintain a normal diploid karyotype indefinitely during culture in vitro and to express marker proteins that are characteristic of ES cells from mice, namely, alkaline phosphatase (AP), stage-specific embryonic antigen-1 (SSEA-1), STAT-3, and Oct 4. After prol...

journal_title：Biochemical and biophysical research communications

authors： Saito S,Sawai K,Ugai H,Moriyasu S,Minamihashi A,Yamamoto Y,Hirayama H,Kageyama S,Pan J,Murata T,Kobayashi Y,Obata Y,Yokoyama KK

更新日期：2003-09-12 00:00:00

abstract：:In the present studies, cAMP, a secondary messenger historically viewed as a negative mediator of immune responses, was demonstrated to possess immunoenhancing activity at low concentrations. In parallel experiments the adenylate cyclase inhibitor, 2',3'-dideoxyadenosine, produced a marked inhibition of humoral and pr...

journal_title：Biochemical and biophysical research communications

authors： Koh WS,Yang KH,Kaminski NE

更新日期：1995-01-17 00:00:00

abstract：:We previously described the isolation of CDK4, representing the 3(')end of an unknown mRNA, using differential display [Biophys. Res. Commun. 232 (1997) 49]. To determine the identity of the CDK4 gene, full-length CDK4 cDNA clones were isolated from a diabetic kidney cDNA library. DNA sequencing revealed that CDK4 rep...

journal_title：Biochemical and biophysical research communications

authors： Page RA,Malik AN

更新日期：2003-10-17 00:00:00

abstract：:A PCR-based Sma I restriction fragment length polymorphism (RFLP) in the human D4 dopamine receptor (DRD4) gene was investigated in 200 subjects each of Japanese and Mongolian populations. When compared to allele frequencies for Caucasians previously reported by Petronis et al., Japanese and Mongolian populations show...

journal_title：Biochemical and biophysical research communications

authors： Nakatome M,Honda K,Tun Z,Wakasugi C

更新日期：1996-03-27 00:00:00

abstract：:Oct4-Sox2-Nanog transcriptional networks are critical for the maintenance of embryonic stem (ES) cell self-renewal and induction of pluripotency. However, in transcription factor-induced reprogramming of somatic cells into induced pluripotent stem cells (iPSCs), Nanog is initially dispensable and Oct4 remains the sole...

journal_title：Biochemical and biophysical research communications

authors： Moon JH,Yun W,Kim J,Hyeon S,Kang PJ,Park G,Kim A,Oh S,Whang KY,Kim DW,Yoon BS,You S

更新日期：2013-02-15 00:00:00

abstract：:Infiltration of the tumor microenvironment by macrophages is associated with poor outcomes in breast cancer and other solid tumors, however the identity and roles of many of the soluble factors these macrophages produce remains to be elucidated in detail. In addition to producing angiogenic factors (e.g. VEGF), protea...

journal_title：Biochemical and biophysical research communications

authors： Levano KS,Jung EH,Kenny PA

更新日期：2011-07-22 00:00:00

abstract：:Heat shock proteins are detectable in human atherosclerotic plaques, especially in endothelial cells. In this report we show by immunofluorescence that incubation "in vitro" with OxLDL is a stress capable of inducing the expression of heat shock protein 70 in both the EAhy-926 cell line and human umbilical vein endoth...

journal_title：Biochemical and biophysical research communications

authors： Zhu W,Roma P,Pellegatta F,Catapano AL

更新日期：1994-04-15 00:00:00

abstract：:Dopamine transporter (DAT) plays a key role in terminating synaptic dopaminergic transmission. DAT acts exclusively on the plasma membrane of presynaptic dopaminergic neurons and DAT gene is an appropriate model for the study of dopaminergic neuron-specific regulation of gene activity. DAT represents an important targ...

journal_title：Biochemical and biophysical research communications

authors： Kouzmenko AP,Pereira AM,Singh BS

更新日期：1997-11-26 00:00:00

abstract：:To assess the relationship between cell proliferation and cell death, the mRNA accumulation of ornithine decarboxylase (ODC) and sulfated glycoprotein 2 (SGP-2) were measured in human peripheral blood lymphocytes (HPBL) 2-6 hours after stimulation with phytohemagglutinin (PHA). ODC is the rate limiting enzyme of polya...

journal_title：Biochemical and biophysical research communications

authors： Grassilli E,Bettuzzi S,Monti D,Ingletti MC,Franceschi C,Corti A

更新日期：1991-10-15 00:00:00

abstract：:Formate dehydrogenase ( FDH ) from Clostridium thermoaceticum is a known tungsten enzyme. FDH was tested for the presence of nitrogenase-type cofactor and nitrate reductase-type cofactor by the Azotobacter vinelandii UW-45 and Neurospora crassa nit-1 reconstitution assays, respectively. Tungsten formate dehydrogenase ...

journal_title：Biochemical and biophysical research communications

authors： Deaton JC,Solomon EI,Durfor CN,Wetherbee PJ,Burgess BK,Jacobs DB

更新日期：1984-06-29 00:00:00

abstract：:We examined the intracellular localization of the myosin binding subunit (MBS) of smooth muscle myosin phosphatase. In MDCK cells in a confluent monolayer of polarized epithelial sheet, MBS was concentrated to the cell-cell adhesion sites. Double-immunofluorescence analysis with anti-MBS and anti-beta-catenin antibodi...

journal_title：Biochemical and biophysical research communications

authors： Inagaki N,Nishizawa M,Ito M,Fujioka M,Nakano T,Tsujino S,Matsuzawa K,Kimura K,Kaibuchi K,Inagaki M

更新日期：1997-01-23 00:00:00

abstract：:Recently we have identified mRNA encoding a hitherto unknown mammalian X,K-ATPase beta-subunit expressed predominantly in muscle tissue (Pestov, N. B. et al. (1999) FEBS Lett. 456, 243-248). Here we demonstrate the existence of the predicted protein, designated as beta(m) (beta(muscle)), in human adult skeletal muscle...

journal_title：Biochemical and biophysical research communications

authors： Pestov NB,Korneenko TV,Zhao H,Adams G,Shakhparonov MI,Modyanov NN

更新日期：2000-10-22 00:00:00

abstract：:Oxidosqualene:protostadienol cyclase (OSPC) from the fungus Aspergillus fumigatus, catalyzes the cyclization of (3S)-2,3-oxidosqualene into protosta-17(20)Z,24-dien-3beta-ol which is the precursor of the steroidal antibiotic helvolic acid. To shed light on the structure-function relationship between OSPC and oxidosqua...

journal_title：Biochemical and biophysical research communications

authors： Kimura M,Kushiro T,Shibuya M,Ebizuka Y,Abe I

更新日期：2010-01-01 00:00:00

abstract：:A novel single-chained antifungal protein with a molecular weight of 13 kDa displaying an N-terminal sequence with marked similarity to embryo-abundant protein from the white spruce was isolated from the seeds of Ginkgo biloba using ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blu...

journal_title：Biochemical and biophysical research communications

authors： Wang H,Ng TB

更新日期：2000-12-20 00:00:00

abstract：:We reported previously that GRF stimulates release of neurotensin (NT) by a clonal line of rat C cells (44-2 C). We report here that GRF stimulates calcitonin (CT) and cAMP release in these cells. For release experiments, replicate cultures are incubated for 5-180 min in serum-free defined medium. CT, NT and cAMP are ...

journal_title：Biochemical and biophysical research communications

authors： Zeytin F,Brazeau P

更新日期：1984-09-17 00:00:00

abstract：:Terminal Schwann cells (TSCs) that cover motor neuron terminals are known to play important roles in maintaining neuromuscular junctions, as well as in the repair process after nerve injury. However, molecular characteristics of TSCs remain unknown, because of the difficulties in analyzing them due to their paucity. W...

journal_title：Biochemical and biophysical research communications

authors： Oda R,Yaoi T,Okajima S,Kobashi H,Kubo T,Fushiki S

更新日期：2003-09-05 00:00:00

abstract：:Chronic kidney disease (CKD) has recently emerged as a major risk factor for cardiovascular pathology. CKD patients display accelerated atherosclerotic process, leading to circulatory complications. However, it is currently not clear how uremic conditions accelerate atherosclerosis. Apoptosis is an important homeostat...

journal_title：Biochemical and biophysical research communications

authors： Trécherel E,Godin C,Louandre C,Benchitrit J,Poirot S,Mazière JC,Massy ZA,Galmiche A

更新日期：2012-01-06 00:00:00

abstract：:We report high resolution images of RecA-double stranded (ds) DNA complexes obtained by atomic force microscopy (AFM). When a carbon nanotube (CNT) tip was used, AFM images visualized the 10-nm pitch of RecA-dsDNA complexes and RecA filaments as three-dimensional surface topography without reconstruction analysis. The...

journal_title：Biochemical and biophysical research communications

authors： Umemura K,Komatsu J,Uchihashi T,Choi N,Ikawa S,Nishinaka T,Shibata T,Nakayama Y,Katsura S,Mizuno A,Tokumoto H,Ishikawa M,Kuroda R

更新日期：2001-02-23 00:00:00

abstract：:The wound healing process is initiated as soon as tissue is injured. Herein, we demonstrate that c-fos and c-myc mRNA transcripts are promptly increased in the wounded tissue in vivo and in vitro. A buffer solution from scraped serum-starved quiescent fibroblasts, when added to resting fibroblasts, caused the increase...

journal_title：Biochemical and biophysical research communications

authors： Tsuboi K,Yamaoka S,Maki M,Ohshio G,Tobe T,Hatanaka M

更新日期：1990-05-16 00:00:00

abstract：:Cartilage-derived growth factors, enhance proteoglycan synthesis in cultured chick-embryo chondrocytes, and have almost no effect on cell proliferation. Addition of cartilage derived growth factors to cartilage cells loaded with the fluorescent Ca2+ indicator quin 2, caused a rapid, concentration dependent decrease in...

journal_title：Biochemical and biophysical research communications

authors： Eilam Y,Beit-Or A,Nevo Z

更新日期：1985-10-30 00:00:00

abstract：:The oxidation of arachidonic acid in a superoxide-generating environment results in the formation of a potent chemoattractant that appears to be identical to a chemotactic material generated by hepatocytes when they metabolize alcohol. The product was extracted, chromatographed and characterized by physical methods in...

journal_title：Biochemical and biophysical research communications

authors： Roll FJ,Perez HD,Serhan CN

更新日期：1992-07-15 00:00:00

abstract：BACKGROUND AND AIMS:Concanavalin A (Con A) activates T lymphocytes and induces CD4+ T cell-mediated hepatic injury in mice. Pro-inflammatory cytokines, such as tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), and interleukin-6 (IL-6), are critical mediators in this experimental model. Activation o...

journal_title：Biochemical and biophysical research communications

authors： Odashima M,Otaka M,Jin M,Horikawa Y,Matsuhashi T,Ohba R,Linden J,Watanabe S

更新日期：2006-09-08 00:00:00

abstract：:Very-long-chain acyl-CoA synthetases (VLCS) activate very-long-chain fatty acids (VLCFA) containing 22 or more carbons to their CoA derivatives. We cloned the human ortholog (hVLCS) of the gene encoding the rat liver enzyme (rVLCS). Both hVLCS and rVLCS contain 620 amino acids, are expressed primarily in liver and kid...

journal_title：Biochemical and biophysical research communications

authors： Steinberg SJ,Wang SJ,Kim DG,Mihalik SJ,Watkins PA

更新日期：1999-04-13 00:00:00

abstract：:Norepinephrine (NE) modulates brain functions depending on both the internal and external environment. While the neuromodulatory actions of NE have been well characterized, the response and involvement of cortical astrocytes to physiological noradrenergic systems remain largely unknown, especially at the molecular lev...

journal_title：Biochemical and biophysical research communications

authors： Nuriya M,Morita A,Shinotsuka T,Yamada T,Yasui M

更新日期：2018-10-12 00:00:00

abstract：:Osteoclast activity is enhanced in acidic environments following systemic or local inflammation. However, the regulatory mechanism of receptor activator of NF-κB ligand (RANKL) expression in osteoblasts under acidic conditions is not fully understood. In the present paper, we detected the mRNA expression of the G-prot...

journal_title：Biochemical and biophysical research communications

authors： Okito A,Nakahama K,Akiyama M,Ono T,Morita I

更新日期：2015-03-06 00:00:00

abstract：:Fas-associated protein with death domain (FADD) has been implicated in T lymphocytes, but the nature of FADD-dependent mechanism in early T cell development has not been completely elucidated. In this study, using T-cell specific deletion mice, we observed that FADD deficiency in thymocytes led to increased apoptosis ...

journal_title：Biochemical and biophysical research communications

authors： Zhang XY,Yang BY,Wang JY,Mo X,Zhang J,Hua ZC

更新日期：2014-08-22 00:00:00

abstract：:IL-10 producing B (B10) cells, a subset of regulatory B (Breg) cells, produce IL-10 and play immunosuppressive roles in antitumor immunity. B10 cells are associated with enhanced tumor-aggressiveness and a poorer prognosis. To specifically inhibit the IL-10 secreted by B cells, we constructed the recombinant plasmid p...

journal_title：Biochemical and biophysical research communications

authors： Zhao R,Liu M,Li X,Chen H,Deng J,Huang C,Zhang XL,Pan Q

更新日期：2018-12-02 00:00:00