Phosphorylation pattern of a 25 Kdalton stress protein from rat myoblasts.

Abstract:

:Phosphorylation of a 25 Kdalton nuclear stress protein from rat muscle was examined by two-dimensional gel electrophoresis and one-dimensional peptide mapping. These studies show that three 25 Kdalton stress proteins found in stressed rat myoblasts are actually the same protein with charge variation brought about by multiple phosphorylations. Furthermore, the predominant charge variant of 25 Kdalton protein found in cells is dependent on the intensity of the stress applied to cells.

authors

Kim YJ,Shuman J,Sette M,Przybyla A

doi

10.1016/0006-291x(83)91651-0

subject

Has Abstract

pub_date

1983-12-28 00:00:00

pages

682-7

issue

3

eissn

0006-291X

issn

1090-2104

pii

0006-291X(83)91651-0

journal_volume

117

pub_type

杂志文章
  • A novel engineered interchain disulfide bond in the constant region enhances the thermostability of adalimumab Fab.

    abstract::We constructed a system for expressing the Fab of the therapeutic human monoclonal antibody adalimumab at a yield of 20 mg/L in the methylotrophic yeast Pichia pastoris. To examine the contribution of interchain disulfide bonds to conformational stability, we prepared adalimumab Fab from which the interchain disulfide...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2017.10.140

    authors: Nakamura H,Oda-Ueda N,Ueda T,Ohkuri T

    更新日期:2018-01-01 00:00:00

  • GTP is required to stabilize and display transamidation activity of transglutaminase 2.

    abstract::Transglutaminase 2 (TGase 2) is a bifunctional enzyme that catalyzes calcium-dependent transamidation and GTP binding/hydrolysis. The transamidation activity is proposed to be associated with several neurodegenerative disorders such as Alzheimer's and Hungtinton's disease. However, the regulation mechanism by which TG...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/S0006-291X(02)00582-X

    authors: Jeon JH,Cho SY,Kim CW,Shin DM,Kweon JC,Choi KH,Park SC,Kim IG

    更新日期:2002-06-21 00:00:00

  • Signal peptide of eosinophil cationic protein is toxic to cells lacking signal peptide peptidase.

    abstract::Eosinophil cationic protein (ECP) is a toxin secreted by activated human eosinophils. The properties of mature ECP have been well studied but those of the signal peptide of ECP (ECPsp) are not clear. In this study, several chimeric proteins containing N-terminal fusion of ECPsp were generated, and introduced into Esch...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2004.07.160

    authors: Wu CM,Chang MD

    更新日期:2004-09-17 00:00:00

  • High glucose increases B1-kinin receptor expression and signaling in endothelial cells.

    abstract::The loss of endothelial function is the initiating factor in the development of diabetic vascular disease. Kinins control endothelial function by the activation of two receptors: the B2 which is constitutively expressed, and the B1 which is highly induced in pathological conditions. In the present study, we observed t...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2006.04.127

    authors: Rodriguez AI,Pereira-Flores K,Hernández-Salinas R,Boric MP,Velarde V

    更新日期:2006-06-30 00:00:00

  • Halothane metabolism: Kupffer cells carry and partially process trifluoroacetylated protein adducts.

    abstract::Kupffer cells, prepared 18 h after pretreatment of rats with a single dose of halothane, did carry TFA-adducts which were recognized on Western blots by a anti-TFA-antibody. Based on apparent molecular weight, the pattern of the major TFA-adducts within Kupffer cells was similar to that observed in hepatocytes. When k...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(05)81228-8

    authors: Christen U,Bürgin M,Gut J

    更新日期:1991-02-28 00:00:00

  • Drosophila p24 and Sec22 regulate Wingless trafficking in the early secretory pathway.

    abstract::The Wnt signaling pathway is crucial for development and disease. The regulation of Wnt protein trafficking is one of the pivotal issues in the Wnt research field. Here we performed a genetic screen in Drosophila melanogaster for genes involved in Wingless/Wnt secretion, and identified the p24 protein family members B...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2015.04.151

    authors: Li X,Wu Y,Shen C,Belenkaya TY,Ray L,Lin X

    更新日期:2015-08-07 00:00:00

  • Adrenomedullin stimulates cyclic AMP formation in rat vascular smooth muscle cells.

    abstract::A newly identified human peptide, adrenomedullin (AM) increased cAMP levels with dose and time dependencies in a manner similar to that of human calcitonin gene-related peptide (CGRP) in rat vascular smooth muscle cells (VSMC). The EC50 value of human AM is 2 x 10(-8) M which is slightly higher than that of CGRP (8.5 ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1994.1496

    authors: Ishizaka Y,Ishizaka Y,Tanaka M,Kitamura K,Kangawa K,Minamino N,Matsuo H,Eto T

    更新日期:1994-04-15 00:00:00

  • Carbohydrate composition of serum transferrin isoforms from patients with high alcohol consumption.

    abstract::Normal human serum transferrin is present in several isoforms, due to differences in glycosylation. Transferrin has two potential glycosylation sites, both normally occupied by oligosaccharide chains. Two of the transferrin isoforms, called carbohydrate deficient transferrin, are specifically increased in patients wit...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1995.1656

    authors: Landberg E,Påhlsson P,Lundblad A,Arnetorp A,Jeppsson JO

    更新日期:1995-05-16 00:00:00

  • Cloning, nucleotide sequence and characterization of a New Zealand rabbit metallothionein-I gene.

    abstract::We have isolated a rabbit metallothionein-I gene from a lambda gt10 library. The coding sequence of this gene is interrupted by two introns occurring at amino acid positions 9 1/3 and 30 1/3. Comparison of the promoter sequence of this gene with the promoters of other metallothionein genes identified a number of oligo...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(88)81210-5

    authors: Tam YC,Chopra A,Hassan M,Thirion JP

    更新日期:1988-05-31 00:00:00

  • A novel siRNA validation system for functional screening and identification of effective RNAi probes in mammalian cells.

    abstract::Small interfering RNAs (siRNAs) have become the most powerful and widely used gene silencing reagents for reverse functional genomics and molecular therapeutics. The key challenge for achieving effective gene silencing in particular for the purpose of the therapeutics is primarily dependent on the effectiveness and sp...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2006.05.164

    authors: Hung CF,Lu KC,Cheng TL,Wu RH,Huang LY,Teng CF,Chang WT

    更新日期:2006-08-04 00:00:00

  • Genome editing in pluripotent stem cells: research and therapeutic applications.

    abstract::Recent progress in human pluripotent stem cell (hPSC) and genome editing technologies has opened up new avenues for the investigation of human biology in health and disease as well as the development of therapeutic applications. Gene editing approaches with programmable nucleases have been successfully established in ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章,评审

    doi:10.1016/j.bbrc.2016.02.113

    authors: Deleidi M,Yu C

    更新日期:2016-05-06 00:00:00

  • MicroRNA-185 inhibits the growth and proliferation of osteoblasts in fracture healing by targeting PTH gene through down-regulating Wnt/β -catenin axis: In an animal experiment.

    abstract::Fracture healing is a repair process of a mechanical discontinuity loss of force transmission, and pathological mobility of bone. Increasing evidence suggests that microRNA (miRNA) could regulate chondrocyte, osteoblast, and osteoclast differentiation and function, indicating miRNA as key regulators of bone formation,...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2018.04.138

    authors: Yao CJ,Lv Y,Zhang CJ,Jin JX,Xu LH,Jiang J,Geng B,Li H,Xia YY,Wu M

    更新日期:2018-06-18 00:00:00

  • In vitro processing by signal peptidase I of precursor maltose-binding protein species with alterations in and around the signal peptide.

    abstract::Processing of 37 precursor maltose-binding protein (preMBP) species by purified signal peptidase I (SPase I) was assayed. The in vitro reaction was inefficient compared to processing in Escherichia coli cells. The extent of preMBP processing in vitro was higher when SPase I was present during translation as compared t...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1993.2598

    authors: Talarico TL,Barkocy-Gallagher GA,Ray PH,Bassford PJ Jr

    更新日期:1993-12-30 00:00:00

  • Similar physical and kinetic properties of rat brain synaptic membrane and cytosol phosphoinositide phospholipases C.

    abstract::Phosphoinositide phospholipase C (PLC) was extracted from the synaptic membrane fraction of rat brain by 1% sodium deoxycholate. The molecular weight and sedimentation coefficient of the membrane PLC were about 160,000 and 6.7 as estimated by gel filtration and sucrose density gradient centrifugation, respectively. Th...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(87)91309-x

    authors: Kozawa O,Hoshijima M,Tanimoto T,Ohmori T,Takai Y

    更新日期:1987-05-29 00:00:00

  • Characterization and evolutionary analysis of ent-kaurene synthase like genes from the wild rice species Oryza rufipogon.

    abstract::Cultivated rice (Oryza sativa) possesses various labdane-related diterpene synthase genes, homologs of ent-copalyl diphosphate synthase (CPS) and ent-kaurene synthase (KS) that are responsible for the biosynthesis of phytohormone gibberellins. The CPS homologs and KS like (KSL) homologs successively converted geranylg...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2016.10.062

    authors: Toyomasu T,Miyamoto K,Shenton MR,Sakai A,Sugawara C,Horie K,Kawaide H,Hasegawa M,Chuba M,Mitsuhashi W,Yamane H,Kurata N,Okada K

    更新日期:2016-11-18 00:00:00

  • Isolation of endo A cDNA from mouse 8-cell stage embryos.

    abstract::To analyse the species of genes expressed in a cleavage stage mouse embryo, we have constructed a cDNA library containing 3.0 x 10(5) independent clones from about 2 x 10(3) embryos at the 8-cell stage of development. Endo A cDNA prepared from parietal yolk sac endoderm like PYS-2 cells was used to screen the library....

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(88)90223-9

    authors: Nozaki M,Murata K,Morita T,Matsushiro A

    更新日期:1988-08-15 00:00:00

  • Functional dominant-negative mutation of sodium channel subunit gene SCN3B associated with atrial fibrillation in a Chinese GeneID population.

    abstract::Atrial fibrillation (AF) is the most common cardiac arrhythmia in the clinic, and accounts for more than 15% of strokes. Mutations in cardiac sodium channel alpha, beta1 and beta2 subunit genes (SCN5A, SCN1B, and SCN2B) have been identified in AF patients. We hypothesize that mutations in the sodium channel beta3 subu...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2010.06.042

    authors: Wang P,Yang Q,Wu X,Yang Y,Shi L,Wang C,Wu G,Xia Y,Yang B,Zhang R,Xu C,Cheng X,Li S,Zhao Y,Fu F,Liao Y,Fang F,Chen Q,Tu X,Wang QK

    更新日期:2010-07-16 00:00:00

  • Activation of farnesoid X receptor induces RECK expression in mouse liver.

    abstract::Farnesoid X receptor (FXR) belongs to the ligand-activated nuclear receptor superfamily, and functions as a transcription factor regulating the transcription of numerous genes involved in bile acid homeostasis, lipoprotein and glucose metabolism. In the present study, we identified RECK, a membrane-anchored inhibitor ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2013.11.082

    authors: Peng X,Wu W,Zhu B,Sun Z,Ji L,Ruan Y,Zhou M,Zhou L,Gu J

    更新日期:2014-01-03 00:00:00

  • Characterization of a mutant GLUT4 lacking the N-glycosylation site: studies in transfected rat adipose cells.

    abstract::GLUT4, the insulin-responsive glucose transporter expressed primarily in muscle and adipose tissue, contains a single N-glycosylation site. We characterized a mutant GLUT4 lacking the N-glycosylation site (Asn57-->Gln) in primary cultures of rat adipose cells. We transiently transfected cells with expression vectors f...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1996.0015

    authors: Ing BL,Chen H,Robinson KA,Buse MG,Quon MJ

    更新日期:1996-01-05 00:00:00

  • Specific uptake of asialofetuin-tacked liposomes encapsulating interferon-gamma by human hepatoma cells and its inhibitory effect on hepatitis B virus replication.

    abstract::Asialofetuin-tacked liposomes (AF-liposomes) encapsulating interferon (IFN)-gamma were bound and internalized into a human hepatoma cell line, HepG2 cells, selectively through asialoglycoprotein receptor, but not non-tacked liposomes (N-liposomes). AF-liposomal IFN-gamma was more effective for inhibition of viral DNA ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(91)91494-w

    authors: Ishihara H,Hayashi Y,Hara T,Aramaki Y,Tsuchiya S,Koike K

    更新日期:1991-01-31 00:00:00

  • Rat insulinoma-derived pancreatic beta-cells express a functional leptin receptor that mediates a proliferative response.

    abstract::In addition to its interaction at hypothalamic sites to affect feeding and energy expenditure, leptin has been shown to exhibit a proliferative response in erythropoietic cells. The functional leptin receptor is also present in pancreatic islets and we now demonstrate that a commonly used clonal insulin secreting beta...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1997.7399

    authors: Islam MS,Morton NM,Hansson A,Emilsson V

    更新日期:1997-09-29 00:00:00

  • Plant sterols in food: no consensus in guidelines.

    abstract::Plant sterols are supplemented in foods to reduce cardiovascular risk. Randomized controlled trials show 2 g of plant sterols a day reduce serum cholesterol by about 10%. This reduction in serum cholesterol levels is achieved at the expense of increased serum plant sterol levels. Findings in patients with phytosterole...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章,评审

    doi:10.1016/j.bbrc.2014.01.147

    authors: Weingärtner O,Baber R,Teupser D

    更新日期:2014-04-11 00:00:00

  • Glycated albumin and cross-linking of CD44 induce scavenger receptor expression and uptake of oxidized LDL in human monocytes.

    abstract::Functional role of CD44, a principal receptor of hyaluronan, and glycated albumin for differentiation of resting human monocytes isolated by counterflow centrifugal elutriation was investigated. Flow cytometric analysis revealed that amadori-modified glycated albumin induced expression of CD44 as well as macrophage sc...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2005.11.091

    authors: Kishikawa H,Mine S,Kawahara C,Tabata T,Hirose A,Okada Y,Tanaka Y

    更新日期:2006-01-20 00:00:00

  • A unique EF-hand motif in mnemiopsin photoprotein from Mnemiopsis leidyi: implication for its low calcium sensitivity.

    abstract::Up to now, all reported Ca(2+)-regulated photoproteins, except for mnemiopsin, have been cloned and expressed in Escherichia coli. In this study, the cDNA for an isotype of mnemiopsin, from the ctenophore Mnemiopsis leidyi, has been cloned, sequenced, and functionally expressed. The full length cDNA encoding mnemiopsi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2011.08.022

    authors: Jafarian V,Sariri R,Hosseinkhani S,Aghamaali MR,Sajedi RH,Taghdir M,Hassannia S

    更新日期:2011-09-23 00:00:00

  • Characterization of p75(+) ectomesenchymal stem cells from rat embryonic facial process tissue.

    abstract::Several populations of stem cells, including those from the dental pulp and periodontal ligament, have been isolated from different parts of the tooth and periodontium. The characteristics of such stem cells have been reported as well. However, as a common progenitor of these cells, ectomesenchymal stem cells (EMSCs),...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2012.08.109

    authors: Wen X,Liu L,Deng M,Zhang L,Liu R,Xing Y,Zhou X,Nie X

    更新日期:2012-10-12 00:00:00

  • Hypertonicity increases sodium transporters in cortical collecting duct cells independently of PGE2.

    abstract::Cyclooxygenase-2 (COX-2) expression is increased by hypertonicity. Therefore we hypothesized that hypertonicity increased PGE(2) can modulate the sodium transporters (Na(+)/K(+)-ATPase: NKA, epithelial sodium channel: ENaC, and sodium hydrogen exchanger: NHE) in M1 cortical collecting duct (CCD) cells. We demonstrated...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2012.01.028

    authors: Nasrallah R,Paris G,Hébert RL

    更新日期:2012-02-10 00:00:00

  • Triiodothyronine decreases the production of androgen binding protein by rat Sertoli cells.

    abstract::Triiodothyronine (T3) effects on cultured Sertoli cells from immature rats were investigated by evaluating the production of androgen binding protein (ABP) a biochemical marker of Sertoli cell function. The results demonstrate that T3 administration to the rat as well as T3 addition to the culture medium specifically ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(87)90656-5

    authors: Fugassa E,Palmero S,Gallo G

    更新日期:1987-02-27 00:00:00

  • Oxidative stress increases gene expression of heparin-binding EGF-like growth factor and amphiregulin in cultured rat gastric epithelial cells.

    abstract::We investigated the effects of oxidative stress on mRNA levels of heparin-binding epidermal growth factor-like growth factor (HB-EGF) and amphiregulin (AR) in rat gastric epithelial RGM1 cells. In response to stimulation with hydrogen peroxide (100-400 microM), gene expression of HB-EGF and AR increased in a dose-depe...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1996.1391

    authors: Miyazaki Y,Shinomura Y,Tsutsui S,Yasunaga Y,Zushi S,Higashiyama S,Taniguchi N,Matsuzawa Y

    更新日期:1996-09-13 00:00:00

  • Distinct atrial natriuretic factor receptor sites on cultured bovine aortic smooth muscle and endothelial cells.

    abstract::Cultured bovine aortic smooth muscle and endothelial cells each display distinct specific binding sites for radiolabeled atrial natriuretic peptide (ANF). 125I-pro-rANF (103-126)I binding to both cell types is rapid, reversible and competitive. Scatchard plots of the binding data show Bmax values of 5.5 and 0.1 - 2.1 ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(85)80179-0

    authors: Schenk DB,Johnson LK,Schwartz K,Sista H,Scarborough RM,Lewicki JA

    更新日期:1985-03-15 00:00:00

  • Cyclin A regulates a cell-cycle-dependent expression of CKAP2 through phosphorylation of Sp1.

    abstract::CKAP2 plays crucial roles in proper chromosome segregation and maintaining genomic stability. CKAP2 protein showed cell-cycle-dependent expression, which reached a maximum level at the G2/M phase and disappeared at the onset of G1 phase. To elucidate the mechanisms underlying cell cycle-dependent expression of CKAP2, ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2012.03.081

    authors: Kang DS,Hong KM,Park J,Bae CD

    更新日期:2012-04-20 00:00:00