当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Assessment of DNA synthesis in Islet-1⁺ cells in the adult murine heart.

Assessment of DNA synthesis in Islet-1⁺ cells in the adult murine heart.

Abstract：

RATIONALE:Islet-1 positive (Islet-1(+)) cardiac progenitor cells give rise to the right ventricle, atria and outflow tract during murine cardiac development. In the adult heart Islet-1 expression is limited to parasympathetic neurons, few cardiomyocytes, smooth muscle cells, within the proximal aorta and pulmonary artery and sinoatrial node cells. Its role in these cells is unknown. Here we tested the hypothesis that Islet-1(+) cells retain proliferative activity and may therefore play a role in regenerating specialized regions in the heart. METHODS AND RESULTS:DNA synthesis was analyzed by the incorporation of tritiated thymidine ((3)H-thymidine) in Isl-1-nLacZ mice, a transgenic model with an insertion of a nuclear beta-galactosidase in the Islet-1 locus. Mice received daily injections of (3)H-thymidine for 5 days. DNA synthesis was visualized throughout the heart by dipping autoradiography of cryosections. Colocalization of an nLacZ-signal and silver grains would indicate DNA synthesis in Islet-1(+) cells. Whereas Islet(-) non-myocyte nuclei were regularly marked by accumulation of silver grains, colocalization with nLacZ-signals was not detected in >25,000 cells analyzed. CONCLUSIONS:Islet-1(+) cells are quiescent in the adult heart, suggesting that, under normal conditions, even pacemaking cells do not proliferate at higher rates than normal cardiac myocytes.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Weinberger F,Mehrkens D,Starbatty J,Nicol P,Eschenhagen T

doi

10.1016/j.bbrc.2014.11.074

subject

Has Abstract

pub_date

2015-01-02 00:00:00

pages

294-7

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(14)02105-6

journal_volume

456

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Glycosylated human growth hormone (hGH): a novel 24 kDa hGH-N variant.

    abstract：:We have identified a human pituitary protein as a novel glycosylated variant of hGH. Isolation of the denatured protein included separation of human pituitary extract by Sephadex G-100 chromatography in ammonium bicarbonate, followed by Sephadex G-100 chromatography in 10% acetic acid, with subsequent DEAE Sephacryl c...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.1697

    authors： Haro LS,Lewis UJ,Garcia M,Bustamante J,Martinez AO,Ling NC

    更新日期：1996-11-12 00:00:00

  • Global cerebral ischemia induces increased expression of multiple retrotransposons.

    abstract：:Retrotransposons (RTs) account for ~45% of the mammalian genome. They are capable of inserting into new genomic locations, which can result in deleterious outcomes. We examined the response of nine RTs to global cerebral ischemia (GCI) and explored the DNA methylation status of the two significantly altered RTs. Seven...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.03.116

    authors： Wang S,Kelly S

    更新日期：2013-05-10 00:00:00

  • Two cis-acting regulatory sequences in the peroxisome proliferator-responsive enhancer region of rat acyl-CoA oxidase gene.

    abstract：:To clarify the mechanism of transcriptional induction of the rat liver acyl-CoA oxidase gene by hypolipidemic agents, we searched for cis-acting regulatory sequences in the 5' upstream region of the gene by transfection studies. We found that the sequence between -639 and -472 acts as a peroxisome proliferator-respons...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)91645-s

    authors： Osumi T,Wen JK,Hashimoto T

    更新日期：1991-03-29 00:00:00

  • Cyclic strain-induced endothelial MMP-2: role in vascular smooth muscle cell migration.

    abstract：:Matrix metalloproteinases (MMPs) play a vital role in vasculature response to hemodynamic stimuli via the degradation of extracellular matrix substrates. In this study, we investigated the putative role of cyclic strain-induced endothelial MMP-2 (and MMP-9) expression and release in modulating bovine aortic smooth mus...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.05.174

    authors： von Offenberg Sweeney N,Cummins PM,Birney YA,Redmond EM,Cahill PA

    更新日期：2004-07-23 00:00:00

  • hnRNP A2 and hnRNP L bind the 3'UTR of glucose transporter 1 mRNA and exist as a complex in vivo.

    abstract：:Recent work identified an RNA binding protein whose presence in brain tumors correlated with translational repression of Glut1 expression. RNase T1 mapping demonstrated that this protein bound an AU-rich response element (AURE) in the Glut1 3'UTR. Facilitated by its differential expression in brain tumor cytosols, we ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1040

    authors： Hamilton BJ,Nichols RC,Tsukamoto H,Boado RJ,Pardridge WM,Rigby WF

    更新日期：1999-08-11 00:00:00

  • Fcf1p and Fcf2p are novel nucleolar Saccharomyces cerevisiae proteins involved in pre-rRNA processing.

    abstract：:The uncharacterized Saccharomyces cerevisiae proteins Fcf1 and Fcf2, encoded by the ORFs YDR339c and YLR051c, respectively, were identified in a tandem affinity purification experiment of the known 40S factor Faf1p. Most of the proteins associated with TAP-Faf1p are trans-acting factors involved in pre-rRNA processing...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.05.140

    authors： Rempola B,Karkusiewicz I,Piekarska I,Rytka J

    更新日期：2006-07-28 00:00:00

  • A 1H NMR study of the solution conformation of the neuropeptide galanin.

    abstract：:The conformations of the neuropeptide galanin in water and trifluoroethanol solutions have been examined by 1H NMR spectroscopy. Analysis of two-dimensional NMR experiments enabled the assignment of virtually all the 1H resonances of galanin in trifluoroethanol solution and many of the 1H resonances in aqueous solutio...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)90980-2

    authors： Wennerberg AB,Cooke RM,Carlquist M,Rigler R,Campbell ID

    更新日期：1990-02-14 00:00:00

  • Depolarizing stimuli reduce Ca(2+)/calmodulin-dependent protein kinase II activity in islets of Langerhans.

    abstract：:Elevations in intracellular Ca(2+) ([Ca(2+)](i)) initiate insulin secretion from pancreatic beta-cells, but the secretory responses become rapidly desensitised to maintained elevations in [Ca(2+)](i). We have investigated the mechanisms underlying the Ca(2+) desensitization of insulin secretion using electrically perm...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.2563

    authors： Harris TE,Persaud SJ,Squires PE,Jones PM

    更新日期：2000-04-21 00:00:00

  • Molecular basis for substrate selectivity of a mono- and diacylglycerol lipase from Malassezia globosa.

    abstract：:The lipase from Malassezia globosa (SMG1) was identified to be strictly specific for mono- and diacylglycerol but not triacylglycerol. The crystal structures of SMG1 were solved in the closed conformation, but they failed to provide direct evidence of factors responsible for this unique selectivity. To address this pr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.06.108

    authors： Liu L,Gao C,Lan D,Yang B,Wang Y

    更新日期：2012-07-27 00:00:00

  • Bradykinin stimulates the tyrosine phosphorylation and bradykinin B2 receptor association of phospholipase C gamma 1 in vascular endothelial cells.

    abstract：:Bradykinin (BK) B2 receptor signaling involves activation of phospholipase C (PLC). PLC activation by other receptors consists of either allosteric activation of PLC beta isoforms by G-proteins or tyrosine phosphorylation of PLC gamma isoforms. Because the B2 receptor is a G-protein-coupled receptor, it has been assum...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.8574

    authors： Venema VJ,Ju H,Sun J,Eaton DC,Marrero MB,Venema RC

    更新日期：1998-05-08 00:00:00

  • The characteristic gene expressions of MAPK phosphatases 1 and 2 in hepatocarcinogenesis, rat ascites hepatoma cells, and regenerating rat liver.

    abstract：:mRNA levels of mitogen-activated protein kinase phosphatases, MKP-1 and MKP-2, were determined during chemical hepatocarcinogenesis and during regeneration of rat liver. In chemical hepatocarcinogenesis, the mRNA levels of MKP-1 were increased in primary hepatomas but decreased in rat ascites hepatomas as compared wit...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.7547

    authors： Yokoyama A,Karasaki H,Urushibara N,Nomoto K,Imai Y,Nakamura K,Mizuno Y,Ogawa K,Kikuchi K

    更新日期：1997-10-29 00:00:00

  • Rv2477c is an antibiotic-sensitive manganese-dependent ABC-F ATPase in Mycobacterium tuberculosis.

    abstract：:The Rv2477c protein of Mycobacterium tuberculosis (Mtb) belongs to the ATP-binding cassette (ABC) subfamily F that contains proteins with tandem nucleotide-binding domains but lacking transmembrane domains. ABC-F subfamily proteins have been implicated in diverse cellular processes such as translation, antibiotic resi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.10.168

    authors： Daniel J,Abraham L,Martin A,Pablo X,Reyes S

    更新日期：2018-01-01 00:00:00

  • The nuclear receptor and clock gene REV-ERBα regulates cigarette smoke-induced lung inflammation.

    abstract：:REV-ERBα is a nuclear heme receptor, transcriptional repressor and critical component of the molecular clock that drives daily rhythms of metabolism. Evidence reveals that REV-ERBα also plays an important regulatory role in clock-dependent lung physiology and inflammatory responses. We hypothesize that cigarette smoke...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.09.157

    authors： Sundar IK,Rashid K,Sellix MT,Rahman I

    更新日期：2017-12-02 00:00:00

  • Genotoxicity and immunogenicity of DNA-advanced glycation end products formed by methylglyoxal and lysine in presence of Cu2+.

    abstract：:The highly reactive electrophile, methylglyoxal (MG), a break down product of carbohydrates, is a major environmental mutagen having potential genotoxic effects. Previous studies have suggested the reaction of MG with free amino groups of proteins forming advanced glycation end products (AGEs). This results in the gen...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.03.064

    authors： Ahmad S,Moinuddin,Dixit K,Shahab U,Alam K,Ali A

    更新日期：2011-04-15 00:00:00

  • Interaction of the two soluble metal-binding domains of yeast Ccc2 with copper(I)-Atx1.

    abstract：:Yeast Ccc2 is a P-type ATPase responsible for transport of copper(I) from the cytosol to the trans-Golgi network. It possesses a soluble cytosolic N-terminal region containing two copper(I)-binding domains. Homologous eukaryotic copper-transporting ATPases have from one to six domains. We have expressed a fragment enc...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.10.045

    authors： Banci L,Bertini I,Chasapis CT,Rosato A,Tenori L

    更新日期：2007-12-21 00:00:00

  • Crystallization and some properties of acetylpolyamine amidohydrolase from Mycoplana bullata.

    abstract：:During the course of investigations on the catabolism of acetylpolyamines by microorganisms, we found that acetylpolyamine amidohydrolase was produced by Mycoplana bullata FERM BP-1845 and isolated the enzyme from the cell-free extract in crystalline form. The enzyme had an apparent molecular weight of 67 kDa and was ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(88)80997-5

    authors： Fujishiro K,Ando M,Uwajima T

    更新日期：1988-12-30 00:00:00

  • Disruption of TGF-β signaling in smooth muscle cell prevents elastase-induced abdominal aortic aneurysm.

    abstract：:Transforming growth factor-β (TGF-β) signaling has been significantly implicated in the pathogenesis of aneurysm, prominently the initiation and progression of abdominal aortic aneurysm (AAA). Vascular smooth muscle cell (SMC) is the principal resident cell in aortic wall and is essential for its structure and functio...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.10.053

    authors： Gao F,Chambon P,Offermanns S,Tellides G,Kong W,Zhang X,Li W

    更新日期：2014-11-07 00:00:00

  • 1,25-Dihydroxyvitamin D3 enhancement of concanavalin-A induced bovine lymphocyte proliferation: requirement of monocytes.

    abstract：:The effect of 1,25-(OH)2D3 on Concanavalin-A (ConA) mediated bovine lymphocyte proliferation was dependent on the magnitude of the proliferative response. Lymphocytes from cows with high inherent proliferative responses (134,904 +/- 15,488 cpm/well) were unaffected by 1,25-(OH)2D3; whereas, lymphocytes from cows with ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(88)80072-x

    authors： Hustmyer FG,Nonnecke BJ,Beitz DC,Horst RL,Reinhardt TA

    更新日期：1988-04-29 00:00:00

  • Stimulation of free radical generation in human leukocytes by various agents including tumor necrosis factor is a calmodulin dependent process.

    abstract：:The mechanism(s) involved in the generation of free radicals in human leukocytes by phorbol myristate acetate (PMA), formyl-methionyl-leucyl-phenylalanine (FMP), lipopolysaccharide (LPS), arachidonic acid (AA), and recombinant-tumor necrosis factor-1-alpha (r-TNF-1 alpha) was investigated. Calmodulin antagonists, chlo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)90626-x

    authors： Das UN,Padma M,Sagar PS,Ramesh G,Koratkar R

    更新日期：1990-03-30 00:00:00

  • Interactions in the active site of Deinococcus radiodurans RNA polymerase during RNA proofreading.

    abstract：:Co-transcriptional RNA proofreading by RNA polymerase (RNAP) is essential for accurate mRNA synthesis and reactivation of stalled transcription complexes, which can otherwise compromise genome integrity. RNAP from the stress-resistant bacterium Deinococcus radiodurans exhibits high levels of RNA cleavage in comparison...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.12.095

    authors： Esyunina D,Kulbachinskiy A

    更新日期：2019-01-29 00:00:00

  • An inhibitor of c-Jun N-terminal kinases (CEP-11004) counteracts the anti-HIV-1 action of trichosanthin.

    abstract：:Trichosanthin (TCS) is a type I ribosome-inactivating protein possessing multiple biological and pharmacological activities. One of its major actions is inhibition of human immunodeficiency virus (HIV) replication. The mechanism is still not clear. It is generally believed that this action is mediated via ribosome ina...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.11.004

    authors： Ouyang DY,Chan H,Wang YY,Huang H,Tam SC,Zheng YT

    更新日期：2006-01-06 00:00:00

  • LncRNA PCAT6 promotes tumor progression in osteosarcoma via activation of TGF-β pathway by sponging miR-185-5p.

    abstract：:Long noncoding RNAs (lncRNAs) play crucial roles in tumor development of osteosarcoma (OS). LncRNA PCAT6 was involved in the progression of multiple human cancers. However, the biological function of PCAT6 in OS remains largely unknown. We found that PCAT6 was elevated in OS tissues relative to that in their adjacent ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.10.136

    authors： Zhu C,Huang L,Xu F,Li P,Li P,Hu F

    更新日期：2020-01-08 00:00:00

  • Cytokine secretion by human adipocytes is differentially regulated by adiponectin, AICAR, and troglitazone.

    abstract：:Adipose tissue is an active endocrine organ producing a variety of cytokines and chemokines, which may be involved in the deregulation of glucose and lipid homeostasis as well as in the inflammatory state observed in obesity. We have shown previously that differentiated human adipocytes secrete a variety of cytokines ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.03.010

    authors： Sell H,Dietze-Schroeder D,Eckardt K,Eckel J

    更新日期：2006-05-12 00:00:00

  • Appearance of membrane-bound tyrosine kinase during differentiation of HL-60 leukemia cells by immune interferon and tumor necrosis factor.

    abstract：:The effect of immune interferon (IFN-gamma) and recombinant tumor necrosis factor (rTNF-alpha) on cellular differentiation was investigated in human promyelocytic leukemia cell line HL-60. Both IFN-gamma and rTNF-alpha induced the appearance of the monocytic phenotype in a dose- and time-dependent manner as assessed b...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(86)90721-7

    authors： Glazer RI,Chapekar MS,Hartman KD,Knode MC

    更新日期：1986-11-14 00:00:00

  • Structural studies on Allium cepa L. chromatin: enhanced stability of internucleosome interactions in plant chromatin.

    abstract：:The pattern of micrococcal nuclease digestion of chromatin from different organs of Allium cepa has been studied. The DNA from small oligonucleosomes appears to be highly degraded and heterogeneous. In solutions of intermediate ionic strength (0.15 M NaCl) histones H2A and H2B form dimers, however at high salt concent...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(85)91940-0

    authors： Moreno ML,Puigdomenech P,Palau J

    更新日期：1985-06-28 00:00:00

  • The miR-599 promotes non-small cell lung cancer cell invasion via SATB2.

    abstract：:MicroRNAs (miRNAs) play important roles in the pathogenesis of many types of cancers by negatively regulating gene expression at posttranscriptional level. Here, we identified that miR-599 is up-regulated in non-small cell lung cancer (NSCLC) patients. It promoted NSCLC cell proliferation by negatively regulating SATB...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章,收录出版

    doi：10.1016/j.bbrc.2017.02.005

    authors： Tian W,Wang G,Liu Y,Huang Z,Zhang C,Ning K,Yu C,Shen Y,Wang M,Li Y,Wang Y,Zhang B,Zhao Y

    更新日期：2017-03-25 00:00:00

  • Reduction in tumor necrosis factor receptor affinity and cytotoxicity by glucocorticoids.

    abstract：:Micromolar concentrations of glucocorticoids rendered L-M cells (a murine tumorigenic fibroblast line) less sensitive to the cytotoxic activity of murine TNF. The potency of different steroids paralleled their known anti-inflammatory potency, and pretreatment was more effective than post treatment. Sex steroids and mi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(88)81238-5

    authors： Kull FC Jr

    更新日期：1988-05-31 00:00:00

  • Multiple replication origins of the archaeon Halobacterium species NRC-1.

    abstract：:The genomic sequence of the halophilic archaeon Halobacterium NRC-1 has been analyzed by the Z curve method. The Z curve is a three-dimensional curve that uniquely represents a given DNA sequence. Based on the known behaviors of the Z curves for the archaea whose replication origins have been identified, the analysis ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(03)00252-3

    authors： Zhang R,Zhang CT

    更新日期：2003-03-21 00:00:00

  • A cancer/testis antigen, NY-SAR-35, induces EpCAM, CD44, and CD133, and activates ERK in HEK293 cells.

    abstract：:The cancer/testis (CT) antigen NY-SAR-35 gene is located on the X chromosome and is aberrantly expressed in various cancers but not in normal tissues, other than testes. Previously, we reported the expression of NY-SAR-35 enhanced cell growth, proliferation, and invasion in HEK293 and cancer cells. To extend understan...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.01.105

    authors： Song MH,Kim YR,Bae JH,Shin DH,Lee SY

    更新日期：2017-03-04 00:00:00

  • Activated nuclear transcription factor kappaB in patients with myocarditis and dilated cardiomyopathy--relation to inflammation and cardiac function.

    abstract：OBJECTIVES AND BACKGROUND:Myocarditis is caused by various agents and autoimmune processes. It is unknown whether viral genome persistence represents inactive remnants of previous infections or whether it is attributed to ongoing adverse processes. The latter also applies to the course of autoimmune myocarditis. One pr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.10.195

    authors： Alter P,Rupp H,Maisch B

    更新日期：2006-01-06 00:00:00