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Two novel RNA binding proteins from Trypanosoma brucei are associated with 5S rRNA.

Abstract：

:We have previously reported the identification of two closely related RNA binding proteins from Trypanosoma brucei which we have termed p34 and p37. The predicted primary structures of the two proteins are highly homologous with one major difference, an 18-amino-acid insert in the N-terminal region of p37. These two proteins have been localized to the nucleus based on immunofluorescence microscopy. To gain insight into their function, we have utilized UV crosslinking, coimmunoprecipitation, and sucrose density gradients to identify T. brucei RNA species that associate with p34 and p37. These experiments have demonstrated a specific interaction of both p34 and p37 with the 5S ribosomal RNA and indicate that other RNA species are unlikely to be specifically bound. This suggests a role for p34 and p37 in the import and/or assembly pathway of T. brucei 5S rRNA in ribosome biogenesis.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Pitula J,Ruyechan WT,Williams N

doi

10.1006/bbrc.2001.6226

subject

Has Abstract

pub_date

2002-01-11 00:00:00

pages

569-76

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006291X01962266

journal_volume

290

pub_type

杂志文章

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