当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Kinetics of lipid-membrane binding and conformational change of L-BABP.

Kinetics of lipid-membrane binding and conformational change of L-BABP.

Abstract：

:We designed an experimental approach to differentiate the kinetics of protein binding to a lipid membrane from the kinetics of the associated conformational change in the protein. We measured the fluorescence intensity of the single Trp6 in chicken liver bile acid-binding protein (L-BABP) as a function of time after mixing the protein with lipid membranes. We mixed the protein with pure lipid membranes, with lipid membranes in the presence of a soluble quencher, and with lipid membranes containing a fluorescence quencher attached to the lipid polar head group. We fitted simultaneously the experimental curves to a three-state kinetic model. We conclude that in a first step, the binding of L-BABP to the interfacial region of the anionic lipid polar head groups occurred simultaneously with a conformational change to the partly unfolded state. In a second slower step, Trp6 buried within the polar head group region, releasing contacts with the aqueous phase.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Galassi V,Nolan V,Villarreal MA,Perduca M,Monaco HL,Montich GG

doi

10.1016/j.bbrc.2009.03.103

subject

Has Abstract

pub_date

2009-05-15 00:00:00

pages

771-5

issue

4

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(09)00595-6

journal_volume

382

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Lipid peroxidation as a possible cause of TCDD toxicity.

    abstract：:The target tissues of TCDD, the dysfunctions that result in death in experimental animals, and the ultimate biochemical lesion(s) caused by TCDD are not known despite numerous studies. We have shown by the thiobarbituric acid and conjugated diene methods that TCDD induces hepatic lipid peroxidation in rats. The lipid ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(83)91377-3

    authors： Stohs SJ,Hassan MQ,Murray WJ

    更新日期：1983-03-29 00:00:00

  • Ntg2 of Saccharomyces cerevisiae repairs the oxidation products of 8-hydroxyguanine.

    abstract：:In Escherichia coli, endonuclease III (endo III) repairs the oxidation products of 8-OHGua. However, the corresponding repair enzymes in eukaryotes have not been identified. Here we report that 8-hydroxyguanine (8-OHGua) is highly sensitive to further oxidation. We also show that Ntg2, a functional homolog of endo III...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.5305

    authors： Kim JE,You HJ,Choi JY,Doetsch PW,Kim JS,Chung MH

    更新日期：2001-08-03 00:00:00

  • A novel method to visually determine the intracellular pH of xenografted tumor in vivo by utilizing fluorescent protein as an indicator.

    abstract：:The alkalization of intracellular pH (pHin) advances together with enhancement of aerobic glycolysis within tumor cells (the Warburg effect), and that is responsible for the progression of tumor malignancy together with hypoxia and angiogenesis. But how they correlate each other during tumor growth is poorly understoo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.07.095

    authors： Tanaka S,Harada H,Hiraoka M

    更新日期：2015-09-04 00:00:00

  • Characterization of new D-beta-aspartate-containing proteins in a lens-derived cell line.

    abstract：:Although proteins are generally composed of l-alpha-amino acids, biologically uncommon D-beta-aspartic acid (Asp)-containing proteins have been reported in various tissues from elderly individuals. Our previous study indicated that the N/N1003A cell line, derived from rabbit lens, includes D-beta-Asp-containing protei...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.06.195

    authors： Takata T,Shimo-Oka T,Miki K,Fujii N

    更新日期：2005-09-09 00:00:00

  • Structure-based development of pyridoxal propionate derivatives as specific inhibitors of cathepsin K in vitro and in vivo.

    abstract：:We found that pyridoxal phosphate shows considerable inhibition of cathepsins. CLIK-071, in which the phosphate ester of position 3 of pyridoxal phosphate was replaced by propionate, strongly inhibited cathepsin B. Three new types of synthetic pyridoxal propionate derivatives showing specific inhibition of cathepsin K...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1953

    authors： Katunuma N,Matsui A,Inubushi T,Murata E,Kakegawa H,Ohba Y,Turk D,Turk V,Tada Y,Asao T

    更新日期：2000-01-27 00:00:00

  • Requirement of both tyrosine residues 1330 and 1337 in the C-terminal tail of the RON receptor tyrosine kinase for epithelial cell scattering and migration.

    abstract：:RON is a receptor tyrosine kinase that mediates cell scattering, migration, and tubular formation. This study focused on the function of two tyrosines, Y1330 and Y1337, in the C-terminus of RON in regulating epithelial cell scattering and migration. Substitution of both tyrosine residues with phenylalanine causes comp...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.2011

    authors： Xiao ZQ,Chen YQ,Wang MH

    更新日期：2000-01-19 00:00:00

  • Leber's hereditary optic neuropathy is associated with the mitochondrial ND6 T14484C mutation in three Chinese families.

    abstract：:We report here the clinical, genetic, and molecular characterization of three Chinese families with maternally transmitted Leber's hereditary optic neuropathy (LHON). Clinical and genetic evaluations revealed the variable severity and age-of-onset in visual impairment in these families. In the affected matrilineal rel...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.06.075

    authors： Sun YH,Wei QP,Zhou X,Qian Y,Zhou J,Lu F,Qu J,Guan MX

    更新日期：2006-08-18 00:00:00

  • Differential Ras signaling via the antigen receptor and IL-2 receptor in primary T lymphocytes.

    abstract：:Ras can become activated via multiple distinct receptors in T lymphocytes. However, mechanistic studies of Ras signaling in normal T cells have been hampered by the lack of an efficient technology for gene transfer into resting post-thymic cells. We have overcome this limitation by utilizing adenoviral transduction of...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2003.10.168

    authors： Marks RE,Ho AW,Rivas F,Marshall E,Janardhan S,Gajewski TF

    更新日期：2003-12-19 00:00:00

  • Effect of lithium carbonate on autophagy and proliferative activity of isolated hepatocytes.

    abstract：:The hepatocytes were cultivated in the presence of lithium carbonate (LC) for drugs testing or possible source for transplantation in the treatment of hereditary or terminal liver diseases. The LC, as an inducer of autophagy, is a promising drug for maintaining cell homeostasis and has a significant effect on the ultr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.03.057

    authors： Dossymbekova R,Bgatova N,Tungushbayeva Z,Sharipov K,Taneyeva G,Kydyrbaeva A,Solovieva A

    更新日期：2020-07-23 00:00:00

  • Isolation of a yeast tropomyosin-related cDNA clone that encodes a novel transmembrane protein having a C-terminal highly basic region.

    abstract：:A novel cDNA clone was isolated from a yeast Saccharomyces cerevisiae lambda gt11 cDNA library using rabbit anti-rat tropomyosin (TM30nm) polyclonal antibody (RTM8-2). It consists of an open reading frame of 951 bp, encoding 317 amino acid residues. The putative sequence recognized by RTM8-2 was present in Asn-235 to ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.1381

    authors： Miyado K,Katsuki M,Taniguchi S

    更新日期：1994-03-30 00:00:00

  • Maillard reaction-like lysine modification by a lipid peroxidation product: immunochemical detection of protein-bound 2-hydroxyheptanal in vivo.

    abstract：:2-Hydroxyheptanal (2-HH) is one of the reactive aldehyde species generated during the peroxidation of n-6 polyunsaturated fatty acids, such as linoleic and arachidonic acids. Analogous to the Maillard reaction of reducing sugars, 2-HH readily reacts with lysine epsilon-amino groups. In the present study, to define the...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(03)01412-8

    authors： Itakura K,Furuhata A,Shibata N,Kobayashi M,Uchida K

    更新日期：2003-08-29 00:00:00

  • Oxidized-LDL induce the expression of heat shock protein 70 in human endothelial cells.

    abstract：:Heat shock proteins are detectable in human atherosclerotic plaques, especially in endothelial cells. In this report we show by immunofluorescence that incubation "in vitro" with OxLDL is a stress capable of inducing the expression of heat shock protein 70 in both the EAhy-926 cell line and human umbilical vein endoth...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.1461

    authors： Zhu W,Roma P,Pellegatta F,Catapano AL

    更新日期：1994-04-15 00:00:00

  • Assignment of overlapping (1)H NMR signals in carp seminal plasma by proton-detected 2D C,H correlation spectroscopy.

    abstract：:The (1)H NMR spectrum of the perchloric acid extract of carp seminal plasma was heavily congested. It is demonstrated that proton-detected C,H chemical shift correlation spectroscopy (HSQC, HSQC-TOCSY) allows an unequivocal identification of proline, glutamate, taurine, and methionine sulfoxide, although several key p...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.2250

    authors： Kalic M,Lauterwein J,Büsselmann G,Kamp G

    更新日期：2000-03-05 00:00:00

  • EPO-independent functional EPO receptor in breast cancer enhances estrogen receptor activity and promotes cell proliferation.

    abstract：:The main function of Erythropoietin (EPO) and its receptor (EPOR) is the stimulation of erythropoiesis. Recombinant human EPO (rhEPO) is therefore used to treat anemia in cancer patients. However, clinical trials have indicated that rhEPO treatment might promote tumor progression and has a negative effect on patient s...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.01.165

    authors： Reinbothe S,Larsson AM,Vaapil M,Wigerup C,Sun J,Jögi A,Neumann D,Rönnstrand L,Påhlman S

    更新日期：2014-02-28 00:00:00

  • KSHV vIRF4 enhances BCL6 transcription via downregulation of IRF4 expression.

    abstract：:Primary effusion lymphoma (PEL), strongly linked with latent infection of Kaposi's sarcoma-associated herpesvirus (KSHV), constitutively expresses cellular interferon regulatory factor 4 (IRF4) while suppressing the expression of B cell lymphoma 6 (BCL6). Recently, it was shown that IRF4, a key transcriptional repress...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.01.154

    authors： Yu HR,Kim YJ,Lee HR

    更新日期：2018-02-19 00:00:00

  • Identification and characterization of mouse PSF1-binding protein, SLD5.

    abstract：:Although most somatic cells cannot proliferate, immature cells proliferate continuously to produce mature cells. Recently, we cloned mouse PSF1 from a hematopoietic stem cell specific cDNA library and reported that PSF1 is indispensable for the proliferation of immature cells. To identify the PSF1-binding protein, we ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.11.136

    authors： Kong L,Ueno M,Itoh M,Yoshioka K,Takakura N

    更新日期：2006-01-27 00:00:00

  • Increase in junctional conductance caused by isoproterenol in heart cell pairs is suppressed by cAMP-dependent protein-kinase inhibitor.

    abstract：:The influence of isoproterenol (10(-6) M) on junctional conductance of cell pairs isolated from adult rat ventricle was investigated using two separated voltage-clamp circuits. The drug increased the junctional conductance by 40% (S.E. +/- 11%) (n = 10) within 20 sec. In experiments in which an inhibitor of cAMP depen...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(88)90169-6

    authors： De Mello WC

    更新日期：1988-07-29 00:00:00

  • Simvastatin induces insulin resistance in L6 skeletal muscle myotubes by suppressing insulin signaling, GLUT4 expression and GSK-3β phosphorylation.

    abstract：:Simvastatin is a 3-hydroxy-3-methylglutaryl-CoA reductase inhibitor widely used for the treatment of hypercholesterolemia. Recent data indicates that simvastatin increases the risk of new-onset diabetes by impairing both insulin secretion and insulin sensitivity. However, systematic evaluation of mechanistic pathways ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.10.026

    authors： Yaluri N,Modi S,Kokkola T

    更新日期：2016-11-11 00:00:00

  • Alternative splicing of Ikaros regulates the FUT4/LeX-α5β1 integrin-FAK axis in acute lymphoblastic leukemia.

    abstract：:Unveiling the mechanism of the relapse of acute lymphoblastic leukemia (ALL) is the key to improve the prognosis of ALL and remains a huge challenge. Glycan-based interactions play a vital role in immune surveillance, cell-cell adhesion and cell-matrix interaction, contributing to treatment failure in tumor. However, ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.01.064

    authors： Yi L,Hu Q,Zhou J,Liu Z,Li H

    更新日期：2019-02-26 00:00:00

  • The serine protease HtrA2 cleaves UCH-L1 and inhibits its hydrolase activity: implication in the UCH-L1-mediated cell death.

    abstract：:Ubiquitin (Ub) carboxyl-terminal hydrolase L1 (UCH-L1) has dual functions, such as hydrolase activity on the chemical bonds formed by the C-terminal Gly of Ub and dimerization-dependent ubiquitin ligase activity. Accumulating evidence suggests that dual activities of UCH-L1 were intimately associated with Parkinson's ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.09.148

    authors： Park DW,Nam MK,Rhim H

    更新日期：2011-11-11 00:00:00

  • Vascular smooth muscle cells as target for estrogen.

    abstract：:ER mRNA was detected as 6.0 kb band by Northern blot analysis in vascular smooth muscle cells (VSMC) derived from rat aorta. The presence of ER mRNA in VSMC was confirmed by reverse transcriptase-polymerase chain reaction using specific primers for rat ER cDNA. In addition, the immunocytochemistry of ER was performed ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.2106

    authors： Orimo A,Inoue S,Ikegami A,Hosoi T,Akishita M,Ouchi Y,Muramatsu M,Orimo H

    更新日期：1993-09-15 00:00:00

  • Identification of two novel 5' noncoding exons in human MNB/DYRK gene and alternatively spliced transcripts.

    abstract：:Previously, the comparison of human MNB/DYRK cDNA sequences with publicly available genomic DNA sequence made a conclusion that MNB/DYRK gene consists of 11 exons spanning 100 kb. Here we found a novel human MNB/DYRK cDNA clone that has the 5' end different from any of those reported MNB/DYRK cDNAs. The 5' end of this...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.9392

    authors： Wang J,Kudoh J,Shintani A,Minoshima S,Shimizu N

    更新日期：1998-09-29 00:00:00

  • AMP-activated protein kinase is essential for survival in chronic hypoxia.

    abstract：:This study was undertaken to interrogate cancer cell survival during long-term hypoxic stress. Two systems with relevance to carcinogenesis were employed: Fully transformed BJ cells and a renal carcinoma cell line (786-0). The dynamic of AMPK activity was consistent with a prosurvival role during chronic hypoxia. This...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.03.056

    authors： Borger DR,Gavrilescu LC,Bucur MC,Ivan M,Decaprio JA

    更新日期：2008-05-30 00:00:00

  • Cumene hydroperoxide-supported demethylation reactions catalyzed by cytochrome P450 2B4 lacking the NH2-terminal sequence.

    abstract：:Catalytic activities of cytochrome P450 2B4 lacking NH2-terminal amino acids 2-27 (wt Delta2B4) and that of truncated 2B4 containing a Pro to Ser mutation at position 221 were examined in a system supported by cumene hydroperoxide. Demethylation activities of either truncated 2B4 with N-methylaniline, N,N-dimethylanil...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.0569

    authors： Zhang Y,Pernecky SJ

    更新日期：1999-04-29 00:00:00

  • microRNA-183 plays as oncogenes by increasing cell proliferation, migration and invasion via targeting protein phosphatase 2A in renal cancer cells.

    abstract：:The aim of this study was to investigate the function of miR-183 in renal cancer cells and the mechanisms miR-183 regulates this process. In this study, level of miR-183 in clinical renal cancer specimens was detected by quantitative real-time PCR. miR-183 was up- and down-regulated in two renal cancer cell lines ACHN...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.08.067

    authors： Qiu M,Liu L,Chen L,Tan G,Liang Z,Wang K,Liu J,Chen H

    更新日期：2014-09-12 00:00:00

  • Modulation of fluoropyrimidine metabolism by chlorpromazine.

    abstract：:Chlorpromazine is a potent inhibitor of calmodulin and can alter the accumulation, metabolism, and incorporation into RNA of several fluoropyrimidine drugs. Using S-180 murine sarcoma cells, the accumulation of the base Fluorouracil and its metabolism to ribonucleotides is enhanced by chlorpromazine treatment, as meas...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(86)80596-4

    authors： Tseng A Jr,Brooks M,Cadman E

    更新日期：1986-07-31 00:00:00

  • Cyclosporin A blocks induction of tumor necrosis factor-alpha in human B lymphocytes.

    abstract：:The effects of cyclosporin A (CSP) on tumor necrosis factor-alpha (TNF-alpha) RNA levels and protein production in human B cells and a B cell line were studied. The ability of CSP to block induction of RNA was compared to its inhibition of protein production in response to anti-IgM, phorbol ester (PMA) and platelet-ac...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.2470

    authors： Smith CS,Ortega G,Parker L,Shearer WT

    更新日期：1994-10-14 00:00:00

  • Vacuolar type H(+)-ATPase genes: presence of four genes including pseudogenes for the 16-kDa proteolipid subunit in the human genome.

    abstract：:Genes for the human vacuolar type H(+)-ATPase proteolipid (16-kDa) subunit were cloned and their nucleotide sequences were determined. Comparison of the deduced sequences indicated that at least four genes including pseudogenes are present in the human genome. One of them corresponded to that for the 16-kDa subunit ex...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(92)90562-y

    authors： Hasebe M,Hanada H,Moriyama Y,Maeda M,Futai M

    更新日期：1992-03-16 00:00:00

  • Revisiting the oligomerization mechanism of Vibrio cholerae cytolysin, a beta-barrel pore-forming toxin.

    abstract：:Vibrio cholerae cytolysin (VCC) is a membrane-damaging beta-barrel pore-forming toxin (beta-PFT). VCC causes permeabilization of the target membranes by forming transmembrane oligomeric beta-barrel pores. Oligomerization is a key step in the mode of action of any beta-PFT, including that of VCC. Earlier studies have i...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.05.006

    authors： Rai AK,Chattopadhyay K

    更新日期：2016-06-03 00:00:00

  • Central effects of a novel acylated peptide, ghrelin, on growth hormone release in rats.

    abstract：:Ghrelin, a novel growth-hormone-releasing acylated peptide, was recently isolated from rat stomach by the search of an endogenous ligand to an "orphan" G-protein-coupled-receptor. Ghrelin neuron is present in the arcuate nucleus of rat hypothalamus, but its central effect on growth hormone (GH) release has yet to be c...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.3342

    authors： Date Y,Murakami N,Kojima M,Kuroiwa T,Matsukura S,Kangawa K,Nakazato M

    更新日期：2000-08-28 00:00:00