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Incorporation of the carbocyclic analogue of (E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-triphosphate into a synthetic DNA.

Abstract：

:The carbocyclic analogue of (E)-5-(2-bromovinyl)-2'-deoxyuridine, C-BVDU, is a very potent and selective anti-herpes-virus compound. In order to synthesize and study the properties of a DNA that contains C-BVDU, the 5'-triphosphate, C-BVDUTP was prepared and evaluated as a potential substrate of the E. coli Klenow DNA polymerase enzyme. Although C-BVDUTP proved to be a very poor substrate also of this enzyme, it could be incorporated up to 3.6% into the synthetic DNA, poly(dA-dT, C-BVDU). This level of substitution decreased significantly the template activity for DNA and RNA polymerases, as compared to that of poly(dA-dT).

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Sági J,De Clercq E,Szemzö A,Csárnyi A,Kovács T,Otvös L

doi

10.1016/s0006-291x(87)80184-5

subject

Has Abstract

pub_date

1987-09-30 00:00:00

pages

1105-12

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(87)80184-5

journal_volume

147

pub_type

杂志文章

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