G-protein dissociation, GTP-GDP exchange and GTPase activity in control and PMA treated neutrophils stimulated by fMet-Leu-Phe.

abstract：:Aurora-C, a member of the Aurora kinase family, is implicated in the regulation of mitosis. In contrast to Aurora-A and Aurora-B its cellular localization and functions are poorly characterized. TACC1 protein belongs to the transforming acidic coiled-coil family shown to interact with the Aurora kinases. In the presen...

journal_title：Biochemical and biophysical research communications

authors： Gabillard JC,Ulisse S,Baldini E,Sorrenti S,Cremet JY,Coccaro C,Prigent C,D'Armiento M,Arlot-Bonnemains Y

更新日期：2011-05-20 00:00:00

abstract：:Our previous studies have shown that the liver from Naked Mole Rats (NMRs), a long-lived rodent, has increased proteasome activity and lower levels of protein ubiquitination compared to mice. This suggests that protein quality control might play a role in assuring species longevity. To determine whether enhanced prote...

journal_title：Biochemical and biophysical research communications

authors： Pride H,Yu Z,Sunchu B,Mochnick J,Coles A,Zhang Y,Buffenstein R,Hornsby PJ,Austad SN,Pérez VI

更新日期：2015-02-20 00:00:00

abstract：:Several recent studies have suggested that cancer stem cells (CSCs) are involved in resistance to gefitinib in non-small cell lung cancer (NSCLC). Oct4, a member of the POU-domain transcription factor family, has been shown to be involved in CSC properties of various cancers. We previously reported that Oct4 and the p...

journal_title：Biochemical and biophysical research communications

authors： Kobayashi I,Takahashi F,Nurwidya F,Nara T,Hashimoto M,Murakami A,Yagishita S,Tajima K,Hidayat M,Shimada N,Suina K,Yoshioka Y,Sasaki S,Moriyama M,Moriyama H,Takahashi K

更新日期：2016-04-22 00:00:00

abstract：:In this study, we have examined the synthesis of heat shock protein (HSP70) in leukemia cells from acute myelogenous leukemia (AML) patients and in mononuclear cells from normal individuals, before and after growth factor stimulation. We have shown that the HSP70 protein was expressed in these cells in the absence of ...

journal_title：Biochemical and biophysical research communications

authors： Zhang W,Drach J,Andreeff M,Deisseroth A

更新日期：1992-03-16 00:00:00

abstract：:In this study of lectin-induced apoptosis we found that wheat germ agglutinin (WGA) initiated an accelerated type of programmed cell death developing after only 30 min of incubation with tumor cells. To analyze possible mechanisms, studies were focused using the WGA lectin whose carbohydrate specificity is well define...

journal_title：Biochemical and biophysical research communications

authors： Gastman B,Wang K,Han J,Zhu ZY,Huang X,Wang GQ,Rabinowich H,Gorelik E

更新日期：2004-03-26 00:00:00

abstract：:Imatinib mesylate, commercially known as Gleevec/Glivec, is the first targeted anticancer drug that inhibits activity of the tyrosine kinases, c-ABL, c-KIT, and PDGFR. A number of studies have shown that treatment with imatinib mesylate elicits extracellular signal-related kinase (ERK) activation, which, in turn, has ...

journal_title：Biochemical and biophysical research communications

authors： Lee J,Ko J,Kim HJ,Im H,Kim EJ,Yi JY

更新日期：2018-12-02 00:00:00

abstract：:We herein report two new genes, human neugrin and mouse homologue m-neugrin, found by screening the cDNA library for the human spinal cord. The neugrin mRNA encodes 219 amino acids and its deduced amino acid sequence contains an NLS-like domain. No previously known motif is found in it. m-neugrin mRNA encodes 233 amin...

journal_title：Biochemical and biophysical research communications

authors： Ishigaki S,Niwa J,Yoshihara T,Mitsuma N,Doyu M,Sobue G

更新日期：2000-12-20 00:00:00

abstract：:Intestinal challenge with cholera toxin induces the synthesis of a hormone-like protein which counteracts intestinal hypersecretion. This study shows that the protein also inhibits GABA transport across the plasma membrane of Deiters' cells in rabbits. The inhibitory action of the protein was dose dependent, and 10(3)...

journal_title：Biochemical and biophysical research communications

authors： Lange S,Lönnroth I,Palm A,Hydén H

更新日期：1985-08-15 00:00:00

abstract：:Previously it was known that cytosolic Ca(2+) elevation was involved in regulating UV- or TNFalpha-induced apoptosis. Here, we reported new evidence that mitochondrial Ca(2+) signal is also involved in the apoptotic process. First, using living cell imaging techniques, we observed multiple mitochondrial Ca(2+) spikes ...

journal_title：Biochemical and biophysical research communications

authors： Lao Y,Chang DC

更新日期：2008-08-15 00:00:00

abstract：:The adipose tissue of genetically obese Zucker rats is characterized by coordinated tissue specific overtranscription of a subset of genes related to lipid storage such as Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH). We show that CCAAT/Enhancer Binding Protein alpha (C/EBP alpha) is an activator of GAPDH proximal...

journal_title：Biochemical and biophysical research communications

authors： Rolland V,Le Liepvre X,Houbiguian ML,Lavau M,Dugail I

更新日期：1995-02-15 00:00:00

abstract：:Leukemia inhibitory factor (LIF) is a cytokine, which inhibits angiogenesis and decreases endothelial cell proliferation and migration, suggesting that LIF may modulate vascular tone. In this study, we examined the effects of LIF on the tone of rat arteries. The isometric tension of ring preparations from rat superior...

journal_title：Biochemical and biophysical research communications

authors： Kimura K,Tsuda K,Moriwaki C,Kawabe T,Hamada M,Obana M,Baba A,Hano T,Nishio I

更新日期：2002-06-07 00:00:00

abstract：:In many apoptotic cells, phosphatidylserine (PS), that is normally restricted to the inner membrane layer, is externalized and subsequently recognized by phagocytes. However, it has been unclear whether PS externalization is sufficient for phagocytosis induction. In a cultured cell line undergoing Fas-mediated apoptos...

journal_title：Biochemical and biophysical research communications

authors： Shiratsuchi A,Osada S,Kanazawa S,Nakanishi Y

更新日期：1998-05-19 00:00:00

abstract：:We have shown previously that aspirin (ASA) ingestion by normal human volunteers inhibits peripheral blood monocyte phospholipase C (PLC) activities ex vivo. In order to explore further the mechanism of action of ASA, normal human monocytes and differentiated human U937 cells were treated with ASA and other salicylate...

journal_title：Biochemical and biophysical research communications

authors： Bomalaski JS,Hirata F,Clark MA

更新日期：1986-08-29 00:00:00

abstract：:Prion diseases are characterized by the accumulation of a pathological form of prion protein (PrP(Sc)), which behaves as an infectious agent. Here we developed an in vitro co-culture system to analyze the PrP(Sc) transmission from ScN2a cell, which persistently retains PrP(Sc), to naïve N2a cell. In this cell-to-cell ...

journal_title：Biochemical and biophysical research communications

authors： Tanaka M,Hara H,Nishina H,Hanada K,Hagiwara K,Maehama T

更新日期：2010-07-02 00:00:00

abstract：:The non-structural protein NS2B/NS3 serine-protease complex of the dengue virus (DENV) is required for the maturation of the viral polyprotein. Dissociation of the NS2B cofactor from NS3 diminishes the enzymatic activity of the complex. In this study, we identified a small molecule inhibitor that interferes with the i...

journal_title：Biochemical and biophysical research communications

authors： Pambudi S,Kawashita N,Phanthanawiboon S,Omokoko MD,Masrinoul P,Yamashita A,Limkittikul K,Yasunaga T,Takagi T,Ikuta K,Kurosu T

更新日期：2013-10-25 00:00:00

abstract：:T-type Ca(2+) channels are important regulators of peripheral sensory neuron excitability. Accordingly, T-type Ca(2+) currents are often increased in various pathological pain conditions, such as inflammation or nerve injury. Here we investigated effects of inflammation on functional expression of T-type Ca(2+) channe...

journal_title：Biochemical and biophysical research communications

authors： Huang D,Liang C,Zhang F,Men H,Du X,Gamper N,Zhang H

更新日期：2016-04-29 00:00:00

abstract：:We quantified the damage yields produced in plasmid DNA by γ-irradiation in the presence of low concentrations (10-100 μM) of ascorbic acid, which is a major antioxidant in living systems, to clarify whether it chemically repairs radiation damage in DNA. The yield of DNA single strand breaks induced by irradiation was...

journal_title：Biochemical and biophysical research communications

authors： Hata K,Urushibara A,Yamashita S,Shikazono N,Yokoya A,Katsumura Y

更新日期：2013-05-03 00:00:00

abstract：:The effects of 1 microM concentrations of arachidonic acid hydroperoxide (HPETES) products of 5-, 12- and 15-lipoxygenase on Na+, K(+)-ATPase activity were investigated in synaptosomal membrane preparations from rat cerebral cortex. 5-HPETE inhibited Na+, K(+)-ATPase activity by up to 67 %. In contrast, 12-HPETE and 1...

journal_title：Biochemical and biophysical research communications

authors： Foley TD

更新日期：1997-06-18 00:00:00

abstract：:A novel neuropeptide which stimulates adenylate cyclase in rat anterior pituitary cell cultures was isolated from ovine hypothalamic tissues. Its amino acid sequence was revealed as: His-Ser-Asp-Gly-Ile-Phe-Thr-Asp-Ser-Tyr-Ser-Arg-Tyr-Arg-Lys-Gln- Met-Ala- Val-Lys-Lys-Tyr-Leu-Ala-Ala-Val-Leu-Gly-Lys-Arg-Tyr-Lys-Gln-Ar...

journal_title：Biochemical and biophysical research communications

authors： Miyata A,Arimura A,Dahl RR,Minamino N,Uehara A,Jiang L,Culler MD,Coy DH

更新日期：1989-10-16 00:00:00

abstract：:Cytochrome P450 2J (Cyp2J) subfamilies are recognized as catalysts of arachidonic acid metabolism in extrahepatic tissues of many species. However, to date, no P450 2J have been identified in zebrafish. We describe here a zfCyp2J1 cDNA which encodes a putative protein of 496 amino acids and shares 51%, 51%, 50%, 51% a...

journal_title：Biochemical and biophysical research communications

authors： Wang L,Yao J,Chen L,Chen J,Xue J,Jia W

更新日期：2007-01-26 00:00:00

abstract：:Mouse peritoneal macrophages metabolize dihomogammalinolenic acid (20:3n-6) primarily to 15-hydroxy-8,11,13-eicosatrienoic acid (15-OH-20:3). Since the biological properties of this novel trienoic eicosanoid remain poorly defined, the effects of increasing concentrations of 15-OH-20:3 and its arachidonic acid (20:4n-6...

journal_title：Biochemical and biophysical research communications

authors： Chapkin RS,Miller CC,Somers SD,Erickson KL

更新日期：1988-06-16 00:00:00

abstract：:Insulin degrading enzyme (IDE) is a thiol-dependent metalloendoprotease that is responsible for initiation of cellular insulin degradation. However, its exact mode of action and the factors controlling it are poorly understood. Since IDE is a metal requiring enzyme, we have examined which metal(s) is(are) endogenously...

journal_title：Biochemical and biophysical research communications

authors： Ebrahim A,Hamel FG,Bennett RG,Duckworth WC

更新日期：1991-12-31 00:00:00

abstract：:The mutation responsible for the juvenile form of Batten disease was mapped to a single gene, Cln3 (T. J. Lerner et al. (1995) Cell 82:949-957). Yeast Saccharomyces cerevisiae has an open reading frame, BTN1 (YHC3), that encodes the putative homologue of Cln3p. Primary structure comparison indicates that the human Cln...

journal_title：Biochemical and biophysical research communications

authors： Croopnick JB,Choi HC,Mueller DM

更新日期：1998-09-18 00:00:00

abstract：:We established cell lines that stably express orphan GPCR GPR174 using CHO cells, and studied physiological and pharmacological features of the receptor. GPR174-expressing cells showed cell-cell adhesion with localization of actin filaments to cell membrane, and revealed significant delay of cell proliferation. Since ...

journal_title：Biochemical and biophysical research communications

authors： Sugita K,Yamamura C,Tabata K,Fujita N

更新日期：2013-01-04 00:00:00

abstract：:We have been investigating the efficacy of an intratumoral interferon (IFN)-alpha gene transfer against solid cancers, and found that when the gene is transduced into the subcutaneous tumors, IFN-alpha concentration is markedly increased in the injected tumor but not in the serum. To explain this effective confinement...

journal_title：Biochemical and biophysical research communications

authors： Yoshida K,Kondoh A,Narumi K,Yoshida T,Aoki K

更新日期：2008-11-14 00:00:00

abstract：:The nucleotide sequence for mouse calcitonin was determined from a cDNA obtained using a polymerase chain reaction (PCR) based method, the rapid amplification of cDNA ends (RACE)-PCR. Primers designed from highly conserved regions in the coding sequences of known rat and human calcitonin cDNAs were used to amplify cal...

journal_title：Biochemical and biophysical research communications

authors： Rehli M,Luger K,Beier W,Falk W

更新日期：1996-09-13 00:00:00

abstract：:CAP is a multifunctional protein; the N-terminal region binds adenylyl cyclase and controls its response to Ras while the C-terminal region is involved in cytoskeletal regulation. In between the two regions, CAP possesses two proline-rich segments, P1 and P2, resembling a consensus sequence for binding SH3 domains. We...

journal_title：Biochemical and biophysical research communications

authors： Yanagihara C,Shinkai M,Kariya K,Yamawaki-Kataoka Y,Hu CD,Masuda T,Kataoka T

更新日期：1997-03-17 00:00:00

abstract：:CH31 B lymphomas represent a model for antigen-induced deletional tolerance of immature B lymphocytes, because cross-linking the B cell antigen receptor (BCR) induces G(1) phase arrest and apoptosis. We have recently demonstrated that BCR cross-linking leads to a transient activation of p38 mitogen-activated protein k...

journal_title：Biochemical and biophysical research communications

authors： Swart JM,Chiles TC

更新日期：2000-09-24 00:00:00

abstract：:Cav1.2 (alpha1C) and Cav1.3 (alpha1D) L-type Ca channels are co-expressed in the heart. To date, there are no pharmacological or biophysical tools to separate alpha1D from alpha1C Ca currents (I(Ca-L)) in cardiomyocytes. Here, we established a physiological model to study alpha1D I(Ca-L) in native myocytes using RNA i...

journal_title：Biochemical and biophysical research communications

authors： Karnabi E,Qu Y,Mancarella S,Yue Y,Wadgaonkar R,Boutjdir M

更新日期：2009-07-10 00:00:00

abstract：:Tyrosine protein kinase activity was measured in membranes from DMBA-induced mammary tumors, with Angiotensin II as substrate. The apparent Km for the peptide was 3.3 mM. This enzymatic activity is inhibited by Ca+2; Mn+2 can replace Mg+2 with an increase in the Km for ATP from 47 /microM to 172 microM. The enzymatic ...

journal_title：Biochemical and biophysical research communications

authors： Levy J,Teuerstein I,Marbach M,Radian S,Sharoni Y

更新日期：1984-09-28 00:00:00