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Ex vivo expansion of hematopoietic stem cell by fusion protein TAT-Zfx.

Abstract：

:The relative inability of hemopoietic stem cells (HSCs) to reproduce themselves (self-renew) ex vivo imposes substantial limitations on the current use of HSC transplantation. Recently, the transcription factor Zfx has been demonstrated that played an important in controlling the self-renewal of hematopoietic stem cells. Here, we reported that Zfx could enable high-level expansion of HSCs in vitro, by combination of protein transduction domain, TAT. Furthermore, we also demonstrated that expanded HSCs population retains their normal in vivo potential of pluripotency. It is thus that TAT-Zfx has the potential to expand HSCs significantly in vitro, and will have enormous clinical potentials.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Xu C,Zhang Y,Jiang H

doi

10.1016/j.bbrc.2008.12.125

subject

Has Abstract

pub_date

2009-02-13 00:00:00

pages

771-4

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(08)02548-5

journal_volume

379

pub_type

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