当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Nek2 localizes to multiple sites in mitotic cells, suggesting its involvement in multiple cellular functions during the cell cycle.

Nek2 localizes to multiple sites in mitotic cells, suggesting its involvement in multiple cellular functions during the cell cycle.

Abstract：

:Nek2 is a mammalian protein kinase that is structurally homologous to NIMA, a mitotic regulator in Aspergillus nidulans. To understand the possible cellular processes in which Nek2 participates during the cell cycle, we investigated the expression and subcellular localization of Nek2 in mitotic cells. The Nek2 protein levels were observed to be regulated in a cell cycle stage-specific manner in cultured cells. The cell cycle stage specificity of Nek2 expression was also confirmed in cells undergoing mitosis in vivo. Nek2 proteins were localized in both the nucleus and cytoplasm throughout the cell cycle, but exhibited dynamic changes in distribution, depending on the cell cycle stage. Nek2 was associated with chromosomes from prophase to metaphase and then was dissociated upon entering into anaphase. Nek2 then appeared at the midbody of the cytoplasmic bridge at telophase. Nek2 was also associated with the centrosome throughout the cell cycle as observed previously by others. Additionally, the nuclear localization of Nek2 was increased during S phase. Such dynamic behavior of Nek2 suggests that Nek2 may be a mitotic regulator that is involved in diverse cell cycle events.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Ha Kim Y,Yeol Choi J,Jeong Y,Wolgemuth DJ,Rhee K

doi

10.1006/bbrc.2001.6212

subject

Has Abstract

pub_date

2002-01-18 00:00:00

pages

730-6

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006291X01962126

journal_volume

290

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Rescue of ligand binding of a mutant IGF-I receptor by complementation.

    abstract：:The IGF-I receptor binds IGF-I with complex kinetics characterized by a curvilinear Scatchard plot, suggesting receptor heterogeneity and apparent negative cooperativity. To explore the molecular mechanisms underlying these properties, we have characterized the binding of a hybrid receptor formed from a wild-type rece...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.03.122

    authors： Chakravarty A,Hinrichsen J,Whittaker L,Whittaker J

    更新日期：2005-05-27 00:00:00

  • Differential regulation of host cellular genes by HIV-1 viral protein R (Vpr): cDNA microarray analysis using isogenic virus.

    abstract：:HIV-1 Vpr is a protein with multiple functions. It has been suggested that such pleiotropic effects by a viral protein may be mediated by its association with viral and cellular proteins or through modulation of expression of specific cellular genes. To address this, we used cDNA microarray techniques to analyze the r...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.01.008

    authors： Janket ML,Manickam P,Majumder B,Thotala D,Wagner M,Schafer EA,Collman RG,Srinivasan A,Ayyavoo V

    更新日期：2004-02-20 00:00:00

  • Sequence of a human keratin 13 specific cDNA encompassing coil 1B through the 3' end.

    abstract：:An expression library established in lambda gt11 with cDNA from squamous epithelium of the human upper digestive tract was screened with an antibody raised against keratin 13. A 1.2 kb fragment from the most strongly reacting plaque was sequenced and compared to known type I keratin sequences. The highest degree of ho...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)90847-4

    authors： Schulz P,Wachter E,Hochstrasser K,Wild AG,Mischke D

    更新日期：1989-08-15 00:00:00

  • TGFβ1-induced down-regulation of microRNA-138 contributes to epithelial-mesenchymal transition in primary lung cancer cells.

    abstract：:The existence of cancer stem cells within the tumor could lead to cancer therapy resistance. TGFβ1 is considered as one of the most powerful players in the generation of CSCs through induction of epithelial-mesenchymal transition in different types of cancer including lung cancer, however, the detailed mechanisms by w...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.01.164

    authors： Zhang F,Li T,Han L,Qin P,Wu Z,Xu B,Gao Q,Song Y

    更新日期：2018-02-19 00:00:00

  • A novel protein, MUDENG, induces cell death in cytotoxic T cells.

    abstract：:A screening system comprised of a randomized hybrid-ribozyme library has previously been used to identify pro-death genes in Fas-mediated apoptosis, and short sequence information of candidate genes from this system was previously reported by Kawasaki and Taira [H. Kawasaki, K. Taira, A functional gene discovery in th...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.03.139

    authors： Lee MR,Shin JN,Moon AR,Park SY,Hong G,Lee MJ,Yun CW,Seol DW,Piya S,Bae J,Oh JW,Kim TH

    更新日期：2008-06-06 00:00:00

  • Topographical requirements for delta opioid ligands: presence of a carboxyl group in position 4 is not critical for deltorphin high delta receptor affinity and analgesic activity.

    abstract：:To investigate the role of the carboxyl group in deltorphin molecules, we have synthesized three new analogues in which the acidic amino acid residues in position 4 of the deltorphins were replaced by non-acidic but hydrophilic amino acids residues. The three analogues, [Ser4]-, [Gln4]-, and [Cys4]-deltorphin, all are...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)81335-x

    authors： Misicka A,Lipkowski AW,Fang L,Knapp RJ,Davis P,Kramer T,Burks TF,Yamamura HI,Carr DB,Hruby VJ

    更新日期：1991-11-14 00:00:00

  • Identification of brain-specific angiogenesis inhibitor 2 as an interaction partner of glutaminase interacting protein.

    abstract：:The vast majority of physiological processes in living cells are mediated by protein-protein interactions often specified by particular protein sequence motifs. PDZ domains, composed of 80-100 amino acid residues, are an important class of interaction motif. Among the PDZ-containing proteins, glutaminase interacting p...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.07.029

    authors： Zencir S,Ovee M,Dobson MJ,Banerjee M,Topcu Z,Mohanty S

    更新日期：2011-08-12 00:00:00

  • Aegyptin inhibits collagen-induced coagulation activation in vitro and thromboembolism in vivo.

    abstract：:Aegyptin is a mosquito salivary gland protein and potent inhibitor of platelet aggregation. Aegyptin binds to the von Willebrand factor-binding site on collagen and prevents its interaction with platelets. Because collagen also induces plasma clotting by activation of factor XII, we evaluated the effects of aegyptin o...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.05.082

    authors： Mizurini DM,Francischetti IM,Monteiro RQ

    更新日期：2013-06-28 00:00:00

  • DNA methylation status influences insulin-induced glucose transport in 3T3-L1 adipocytes by mediating p53 expression.

    abstract：:Researchers frequently use 3T3-L1 adipocytes as a fat cell line, but the capacity of this line for insulin-mediated glucose transport is lower than that of primary isolated fat cells. In this study, we found that 5-azacytidine (5-aza-C), DNA methyltransferase 1 inhibitor, enhanced insulin-stimulated 2-deoxyglucose (2-...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.02.038

    authors： Kuroda M,Onoyama R,Sasaki W,Sebe M,Kitamura T,Masumoto S,Tsutsumi R,Harada N,Sakaue H

    更新日期：2020-02-16 00:00:00

  • Prion protein suppresses perturbation of cellular copper homeostasis under oxidative conditions.

    abstract：:Prion protein (PrP) binds copper and exhibits superoxide dismutase-like activity, while the roles of PrP in copper homeostasis remain controversial. Using Zeeman graphite furnace atomic absorption spectroscopy, we quantified copper levels in immortalized PrP gene (Prnp)-deficient neuronal cells transfected with Prnp a...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2003.12.020

    authors： Sakudo A,Lee DC,Yoshimura E,Nagasaka S,Nitta K,Saeki K,Matsumoto Y,Lehmann S,Itohara S,Sakaguchi S,Onodera T

    更新日期：2004-01-23 00:00:00

  • Steroid hormones inhibit binding of Vinca alkaloid to multidrug resistance related P-glycoprotein.

    abstract：:Multidrug-resistant cells are characterized by the presence of P-glycoprotein on the plasma membrane, which binds and probably transports antitumor agents outside the cells. P-glycoprotein is also present in various normal tissues such as the adrenal gland. To investigate the physiological function of P-glycoprotein, ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)92830-1

    authors： Naito M,Yusa K,Tsuruo T

    更新日期：1989-02-15 00:00:00

  • Protein binding domains of the rat thyroglobulin promoter.

    abstract：:We have previously shown that DNA elements controlling tissue specific expression of the rat thyroglobulin gene extend 170 bp upstream of the cap site and have identified a thyroid specific nuclear factor which binds the promoter in the -60 region (site C). Here we report that the distal portion of the promoter, exten...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)92162-1

    authors： Ursini MV,Gallo A,Olivetta E,Musti AM

    更新日期：1989-08-30 00:00:00

  • A novel compound heterozygote (FAP ATTR Arg104His/ATTR Val30Met) with high serum transthyretin (TTR) and retinol binding protein (RBP) levels.

    abstract：:A 64-year-old Japanese male suffering from very slowly progressive amyloidosis was studied by immunohistopathologic, mass spectrometric, and molecular genetic methods. After confirming the immunoreactivity of transthyretin (TTR) in the amyloid deposits using an anti-TTR polyclonal antibody, matrix-assisted laser desor...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1514

    authors： Terazaki H,Ando Y,Misumi S,Nakamura M,Ando E,Matsunaga N,Shoji S,Okuyama M,Ideta H,Nakagawa K,Ishizaki T,Ando M,Saraiva MJ

    更新日期：1999-10-22 00:00:00

  • The effect of anionic detergents on the ATPase activity of isolated F1 from the thermophilic bacterium PS3.

    abstract：:The ATPase activity of F1 isolated from the thermophilic bacterium PS3 is stimulated at 30 degrees C by the anionic detergents cholate or deoxycholate. Maximal activity obtained with these detergents (35 mumol/min X mg) is similar to the activity reported for the optimal temperature, 75 degrees C. The activity is line...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(86)90417-1

    authors： Norling B

    更新日期：1986-05-14 00:00:00

  • The selection of the first AUG as the initiator of eucaryotic mRNAs translation is favored by a 5'-terminal cap group and a purine in the -3' position.

    abstract：:We have constructed a series of plasmids containing variations in the DNA that codes for the translation initiation region of preproparathyroid hormone. Gel electrophoretic analyses of the character and extent of synthesis of various modified preproparathyroid hormone like proteins derived from the mRNAs transcribed f...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(86)91088-0

    authors： Szczesna-Skorupa E,Mead DA,Kemper B

    更新日期：1986-10-15 00:00:00

  • Cleavage of yeast tRNA(phe) with Ni(III) and Co(III) complexes of bleomycin.

    abstract：:The BLM-Ni(III) complex preferentially cleaves guanine residues at D loop and anticodon loop of yeast tRNA(phe) by aniline treatment, whereas the BLM-Co(III) complex degrades certain 5'-AN sites of the minor groove region after irradiation of UV light. These cleavage features are clearly different from that of the BLM...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.1364

    authors： Guan LL,Totsuka R,Kuwahara J,Otsuka M,Sugiura Y

    更新日期：1993-03-31 00:00:00

  • The protective effect of orally administered redox nanoparticle on intestinal ischemia-reperfusion injury in mice.

    abstract：BACKGROUND:Intestinal ischemia-reperfusion (I-R) injury is a serious abdominal condition leading to multiple organ failure with high mortality. However, no reliable treatment is available. A redox nanoparticle (RNPO) was recently developed, and its efficacy for several intestinal inflammatory conditions has been report...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.11.204

    authors： Ueda T,Takagi T,Katada K,Iida T,Mizushima K,Dohi O,Okayama T,Yoshida N,Kamada K,Uchiyama K,Handa O,Ishikawa T,Konishi H,Naito Y,Nagasaki Y,Itoh Y

    更新日期：2018-01-08 00:00:00

  • Biological properties of purified recombinant HCV particles with an epitope-tagged envelope.

    abstract：:To establish a simple system for purification of recombinant infectious hepatitis C virus (HCV) particles, we designed a chimeric J6/JFH-1 virus with a FLAG (FL)-epitope-tagged sequence at the N-terminal region of the E2 hypervariable region-1 (HVR1) gene (J6/JFH-1/1FL). We found that introduction of an adaptive mutat...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.04.081

    authors： Takahashi H,Akazawa D,Kato T,Date T,Shirakura M,Nakamura N,Mochizuki H,Tanaka-Kaneko K,Sata T,Tanaka Y,Mizokami M,Suzuki T,Wakita T

    更新日期：2010-05-14 00:00:00

  • Epidermal growth factor inhibits both cholecystokinin octapeptide-induced inositol 1,4,5-trisphosphate production and [CA2+]i increase in rat pancreatic acinar cells.

    abstract：:We have studied the effects of epidermal growth factor (EGF) on both cholecystokinin octapeptide (CCK-OP)-induced inositol-1,4,5 trisphosphate (IP3) production and on cytosolic free calcium concentrations [Ca2+]i by fluorescence measurements in fura-2-loaded pancreatic acini. Our data show that EGF inhibits CCK-OP ind...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)81150-7

    authors： Pröfrock A,Piiper A,Eckhardt L,Schulz I

    更新日期：1991-10-31 00:00:00

  • Evidence in vitro for an enzymatic synthesis of phosphocitrate.

    abstract：:A biological synthesis of phosphocitrate is described from precursors, citrate and adenosinetriphosphate reacting in the presence of rat liver homogenate. Identity of the newly formed product was examined by enzymatic digestion of reactions mixtures, HPLC chromatography and 1H-NMR spectra. Authenticity of product was ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)91267-v

    authors： Moro L,Stagni N,Luxich E,Sallis JD,de Bernard B

    更新日期：1990-07-16 00:00:00

  • Era, an essential Escherichia coli small G-protein, binds to the 30S ribosomal subunit.

    abstract：:Era is an essential G-protein in Escherichia coli identified originally as a homologue protein to Ras (E. coli Ras-like protein). It binds to GTP/GDP and contains a low intrinsic GTPase activity. Its function remains elusive, although it has been suggested that Era is associated with the cytoplasmic membrane, cell div...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1471

    authors： Sayed A,Matsuyama Si,Inouye M

    更新日期：1999-10-14 00:00:00

  • A point mutation in the glucose-dependent insulinotropic peptide receptor confers constitutive activity.

    abstract：:The glucose-dependent insulinotropic peptide receptor (GIP-R) is a member of the secretin and parathyroid hormone (PTH) family of seven transmembrane-spanning receptors. Point mutations of a histidine at the junction between the first intracellular loop and the second membrane-spanning domain and a threonine in the si...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.6231

    authors： Tseng CC,Lin L

    更新日期：1997-03-06 00:00:00

  • Interaction of rat lin-10 with brain-enriched F-actin-binding protein, neurabin-II/spinophilin.

    abstract：:We have recently isolated a rat homologue of the Caenorrhabditis elegans lin-10 product. Although rat lin-10 is expressed in the cytosol and membrane fractions of various tissues, it is distributed only in the membrane fraction in brain where it is enriched in the synaptic plasma membrane and postsynaptic density frac...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.8243

    authors： Ide N,Hata Y,Hirao K,Irie M,Deguchi M,Yao I,Satoh A,Wada M,Takahashi K,Nakanishi H,Takai Y

    更新日期：1998-03-06 00:00:00

  • Molecular characterization of seizure-related genes isolated by differential screening.

    abstract：:To isolate seizure-related genes, we applied differential screening technique to the cDNA library which constructed from primary cultured cerebral cortical cells treated with pentylenetetrazol (PTZ). Northern blotting analysis of mRNA levels in the cerebra after systemic administration of PTZ confirmed the results of ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.0313

    authors： Kajiwara K,Nagawawa H,Shimizu-Nishikawa S,Ookuri T,Kimura M,Sugaya E

    更新日期：1996-02-27 00:00:00

  • Role of macrophages in inflammatory lymphangiogenesis: Enhanced production of vascular endothelial growth factor C and D through NF-kappaB activation.

    abstract：:The close association of inflammation, angiogenesis and cancer progression is now highlighted, and in this study we especially focused on a close association of inflammation and lymphangiogenesis. We found that proinflammatory cytokine, interleukin-1beta (IL-1beta), could induce lymphangiogenesis in mouse cornea throu...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.10.077

    authors： Watari K,Nakao S,Fotovati A,Basaki Y,Hosoi F,Bereczky B,Higuchi R,Miyamoto T,Kuwano M,Ono M

    更新日期：2008-12-19 00:00:00

  • Knockdown of slincRAD leads to defective adipose development in vivo.

    abstract：:The development of adipose tissue is a precisely coordinated cellular process, in which both protein-coding and non-coding genes are involved. To characterize the in vivo function of a novel long non-coding RNA (lncRNAs), loss-of-function assays were performed with slincRAD knockdown mice. Down-regulation of slincRAD ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.04.035

    authors： Zhang P,Bai H,Li J,Liu J,Ma W,Xu B,Xia Q,Wang J,Fan Yi,Du Q

    更新日期：2019-06-11 00:00:00

  • miR-27a is a negative regulator of adipocyte differentiation via suppressing PPARgamma expression.

    abstract：:microRNAs (miRNAs) are non-coding small RNAs regulating gene expression, cell growth, and differentiation. Although several miRNAs have been implicated in cell growth and differentiation, it is barely understood their roles in adipocyte differentiation. In the present study, we reveal that miR-27a is involved in adipo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.01.012

    authors： Kim SY,Kim AY,Lee HW,Son YH,Lee GY,Lee JW,Lee YS,Kim JB

    更新日期：2010-02-12 00:00:00

  • Subcellular localization of the interaction of bipolar landmarks Bud8p and Bud9p with Rax2p in Saccharomyces cerevisiae diploid cells.

    abstract：:In Saccharomyces cerevisiae, the bud site selection of diploid cells is regulated by at least four persistent landmarks, Bud8p, Bud9p, Rax1p, and Rax2p. Bud8p and Bud9p are essential for the establishment of bipolar budding and localize mainly to the distal and the proximal poles, respectively. Their subcellular local...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.07.102

    authors： Kato Y,Kawasaki H,Arakawa N,Hirano H

    更新日期：2010-09-03 00:00:00

  • Plasma albumin cysteinylation is regulated by cystathionine beta-synthase.

    abstract：:High homocysteine (Hcy) levels are a well-known independent risk factor for endothelial damage in atherosclerosis. We examined whether a rat intestinal model of ischemia-reperfusion was associated with high Hcy and with the modification of plasma albumin into cysteinylated species (CysAlb). The three treatment groups ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.10.191

    authors： Bar-Or D,Curtis CG,Sullivan A,Rael LT,Thomas GW,Craun M,Bar-Or R,Maclean KN,Kraus JP

    更新日期：2004-12-24 00:00:00

  • TNP-470 blockage of VEGF synthesis is dependent on MAPK/COX-2 signaling pathway in PDGF-BB-activated hepatic stellate cells.

    abstract：:Angiogenesis is a key pathogenic event in hepatic fibrogenesis, which is mediated by activated hepatic stellate cells (HSCs). TNP-470 is a known anti-angiogenic agent in cancer, and in this study, we investigated the regulatory mechanisms of TNP-470 blockage of vascular endothelial growth factor (VEGF) synthesis in ac...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.12.175

    authors： Wang YQ,Luk JM,Chu AC,Ikeda K,Man K,Kaneda K,Fan ST

    更新日期：2006-03-03 00:00:00