Abstract:
:We have previously shown that DNA elements controlling tissue specific expression of the rat thyroglobulin gene extend 170 bp upstream of the cap site and have identified a thyroid specific nuclear factor which binds the promoter in the -60 region (site C). Here we report that the distal portion of the promoter, extending from -160 to -120, contains two contiguous DNA elements (sites A and B) which interact with the same thyroid-specific factor binding to proximal site C. A second nuclear factor, ubiquitously distributed, binds to the distal site A. Transient cotransfection-competition studies show that all the three binding sites A, B and C titrate a trans-acting factor(s) which is necessary for the transcription of the thyroglobulin gene.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Ursini MV,Gallo A,Olivetta E,Musti AMdoi
10.1016/0006-291x(89)92162-1subject
Has Abstractpub_date
1989-08-30 00:00:00pages
481-8issue
1eissn
0006-291Xissn
1090-2104pii
0006-291X(89)92162-1journal_volume
163pub_type
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