当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Selective disulfide reduction for labeling and enhancement of Fab antibody fragments.

Selective disulfide reduction for labeling and enhancement of Fab antibody fragments.

Abstract：

:Many methods have been developed for chemical labeling and enhancement of the properties of antibodies and their common fragments, including the Fab and F(ab')2 fragments. Somewhat selective reduction of some antibody disulfide bonds has been previously achieved, yielding antibodies and antibody fragments that can be labeled at defined sites, enhancing their utility and properties. Selective reduction of the two hinge disulfide bonds present in F(ab')2 fragments using mild reduction has been useful. However, such reduction is often not quantitative and results in the reduction of multiple disulfide bonds, and therefore subsequent multiple labeling or conjugation sites are neither homogenous nor stoichiometric. Here, a simple and efficient selective reduction of the single disulfide bond linking the partial heavy chain and the intact light chain which compose the Fab fragment is accomplished utilizing tris(2-carboxyethyl)phosphine (TCEP) immobilized on agarose beads. The resultant reduced cysteine residues were labeled with several cysteine-selective fluorescent reagents, as well as by cysteine-directed PEGylation. These two cysteine residues can also be re-ligated by means of a bifunctional cysteine cross-linking agent, dibromobimane, thereby both restoring a covalent linkage between the heavy and light chains at this site, far removed from the antigen binding site, and also introducing a fluorescent probe. There are many other research and clinical uses for these selectively partially reduced Fab fragments, including biotinylation, toxin and drug conjugation, and incorporation of radioisotopes, and this technique enables simple generation of very useful Fab fragment derivatives with many potential applications.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Kirley TL,Greis KD,Norman AB

doi

10.1016/j.bbrc.2016.10.128

subject

Has Abstract

pub_date

2016-11-25 00:00:00

pages

752-757

issue

4

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(16)31814-9

journal_volume

480

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • NS5A protein of HCV enhances HBV replication and resistance to interferon response.

    abstract：:HCV and HBV are the major causes of chronic liver diseases worldwide. Patients with both viruse's co-infection tend to develop severer liver diseases and are at high risk of liver-related death. NS5A protein of HCV plays key roles in HCV replication and inhibition of host immune responses. In this study, we described ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.05.052

    authors： Pan Y,Wei W,Kang L,Wang Z,Fang J,Zhu Y,Wu J

    更新日期：2007-07-20 00:00:00

  • LeftyA sensitive cytosolic pH regulation and glycolytic flux in Ishikawa human endometrial cancer cells.

    abstract：OBJECTIVE:LeftyA, a powerful regulator of stemness, embryonic differentiation, and reprogramming of cancer cells, counteracts cell proliferation and tumor growth. Key properties of tumor cells include enhanced glycolytic flux, which is highly sensitive to cytosolic pH and thus requires export of H(+) and lactate. H(+) ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.03.120

    authors： Salker MS,Zhou Y,Singh Y,Brosens J,Lang F

    更新日期：2015-05-08 00:00:00

  • Proprotein convertases regulate insulin-like growth factor 1-induced membrane-type 1 matrix metalloproteinase in VSMCs via endoproteolytic activation of the insulin-like growth factor-1 receptor.

    abstract：:The IGF-1 receptor (IGF-1R) and MT1-MMP are synthesized as larger precursor proproteins, which require endoproteolytic activation by the proprotein convertases (PCs) furin/PC5 to gain full biological activity. The aim of this study was to investigate the contribution of PCs to IGF-1R and/or MT1-MMP activation in vascu...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.07.001

    authors： Stawowy P,Kallisch H,Kilimnik A,Margeta C,Seidah NG,Chrétien M,Fleck E,Graf K

    更新日期：2004-08-27 00:00:00

  • Purification and characterization of mouse CYP27B1 overproduced by an Escherichia coli system coexpressing molecular chaperonins GroEL/ES.

    abstract：:The expression of mouse CYP27B1 in Escherichia coli has been dramatically enhanced by coexpression of GroEL/ES. To reveal the enzymatic properties of CYP27B1, we measured its hydroxylation activity toward vitamin D3 and 1alpha-hydroxyvitamin D3 (1alpha(OH)D3) in addition to the physiological substrate 25(OH)D3. Surpri...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.08.110

    authors： Uchida E,Kagawa N,Sakaki T,Urushino N,Sawada N,Kamakura M,Ohta M,Kato S,Inouye K

    更新日期：2004-10-15 00:00:00

  • Membrane phospholipids remodeling upon imbibition in Brassica napus L. seeds.

    abstract：:Successful seed germination depends on the rapid repair of cell membrane damaged by dry storage. However, little is known about the reorganization of lipids during this process. In this study, the changes of intracellular redox environment, cell membrane integrity, lipid composition, and expression of genes related to...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.05.100

    authors： Lin YX,Xin X,Yin GK,He JJ,Zhou YC,Chen JY,Lu XX

    更新日期：2019-07-23 00:00:00

  • Coordinated induction of two unrelated glucose-regulated protein genes by a calcium ionophore: human BiP/GRP78 and GAPDH.

    abstract：:The induction of human BiP/GRP78 and GAPDH protein genes by the calcium ionophore A23187 was determined. Steady-state levels of mRNA for both the glucose starvation-responsive BiP/GRP78 gene and the glucose-responsive GAPDH gene were dramatically induced in a variety of human cells. There is a homologous palindromatic...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)91411-k

    authors： Chao CC,Yam WC,Lin-Chao S

    更新日期：1990-08-31 00:00:00

  • MicroRNA-224 targets RKIP to control cell invasion and expression of metastasis genes in human breast cancer cells.

    abstract：:The Raf kinase inhibitor protein (RKIP) is a tumor suppressor that protects against metastasis and genomic instability. RKIP is downregulated in many types of tumors, although the mechanism for this remains unknown. MicroRNAs silence target genes via translational inhibition or target mRNA degradation, and are thus im...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.07.025

    authors： Huang L,Dai T,Lin X,Zhao X,Chen X,Wang C,Li X,Shen H,Wang X

    更新日期：2012-08-24 00:00:00

  • Intracellular cAMP contents regulate NAMPT expression via induction of C/EBPβ in adipocytes.

    abstract：:A decline in intracellular nicotinamide adenine mononucleotide (NAD+) causes adipose tissue dysfunction. Nicotinamide phosphoribosyltransferase (NAMPT) catalyzes the rate-limiting step in the NAD+ biosynthesis pathway. However, the molecular mechanism that mediates regulation of NAMPT expression in adipocytes is yet t...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.11.165

    authors： Mitani T,Watanabe S,Wada K,Fujii H,Nakamura S,Katayama S

    更新日期：2020-02-12 00:00:00

  • Interleukin-4 stimulates pro-alpha 1(VI) collagen gene expression in cultured human osteoblast-like cells.

    abstract：:Interleukin-4 (IL-4) is a pleiotropic immunomodulatory cytokine produced mainly by T lymphocyte. It is known that many kinds of non-haematopoietic cells, as well as immune cells and haematopoietic cells, express IL-4 receptors. Recently, we found that IL-4 increased hydroxyproline and osteocalcin accumulation and caus...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.1873

    authors： Ishibashi H,Karube S,Yamakawa A,Koshihara Y

    更新日期：1995-06-26 00:00:00

  • Investigation of antigen delivery route in vivo and imune-boosting effects mediated by pH-sensitive liposomes encapsulated with K(b)-restricted CTL epitope.

    abstract：:Using fluorescein isothiocyanate (FITC)-conjugated H-2K(b) CTL epitope (SIINFEKL) as a model system, we investigated the antigen delivery route by pH-sensitive liposomes in vivo. Fluorescence was initially detected in lymph nodes at 3 h after immunization, and its intensity reached a peak value in superticial inguinal...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2002.6711

    authors： Lee KY,Chun E,Seong BL

    更新日期：2002-04-05 00:00:00

  • Identification of parathyroid hormone-regulated proteins in mouse bone marrow cells by proteomics.

    abstract：:The ability of parathyroid hormone (PTH) to enhance bone formation has recently been exploited in the treatment of osteoporosis. Several studies have suggested that the activation of bone marrow stromal cells could be preceded to show the anabolic effect of PTH on bone formation, but little is known of PTH-regulated p...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.02.173

    authors： Kim SH,Jun S,Jang HS,Lim SK

    更新日期：2005-05-06 00:00:00

  • The isolation and in vitro expansion of hepatic Sca-1 progenitor cells.

    abstract：:The intra-hepatic population of liver progenitor cells expands during liver injury when hepatocyte proliferation is inhibited. These cells can be purified by density gradient centrifugation and cultured. Separated by size only this population contains small cells of hematopoietic, epithelial and endothelial lineages a...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.02.079

    authors： Clayton E,Forbes SJ

    更新日期：2009-04-17 00:00:00

  • Thymoquinone inhibits growth and augments 5-fluorouracil-induced apoptosis in gastric cancer cells both in vitro and in vivo.

    abstract：:Thymoquinone (TQ), a component derived from the bioactive constituent of black seed (Nigella sativa), has been shown to exert biological activity on various types of human cancers. However, there are few studies addressing its effects on gastric cancer. Here, we present the first report describing the chemosensitizing...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.12.063

    authors： Lei X,Lv X,Liu M,Yang Z,Ji M,Guo X,Dong W

    更新日期：2012-01-13 00:00:00

  • Effect of EI24 expression on the tumorigenesis of ApcMin/+ colorectal cancer mouse model.

    abstract：:Etoposide-induced 2.4 kb transcript (EI24, also known as PIG8) is a p53 target gene involved in cell growth suppression and apoptosis and known to be frequently altered in human cancers. Although EI24 expression is decreased in various cancers and is associated with colorectal cancer progression and metastasis, the ph...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.04.186

    authors： Nam TW,Park SY,Lee JH,Roh JI,Lee HW

    更新日期：2019-07-05 00:00:00

  • Functional roles of the two distinct domains of halysase, a snake venom metalloprotease, to inhibit human platelet aggregation.

    abstract：:Halysase, a hemorrhagic metalloprotease, has an apparent molecular weight of 66kDa and belongs to the class P-III snake venom metalloprotease. Class P-III snake venom metalloproteases have multifunctional domains including a protease domain and a disintegrin-like domain. Halysase was able to preferentially hydrolyze t...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.11.083

    authors： You WK,Jang YJ,Chung KH,Jeon OH,Kim DS

    更新日期：2006-01-20 00:00:00

  • Insulin-like growth factor I stimulates transcription of the c-jun proto-oncogene in Balb/C 3T3 cells.

    abstract：:Treatment of quiescent Balb/c 3T3 cells with insulin-like growth factor I (IGF I) resulted in the stimulation of proto-oncogene c-jun transcription. Cells exposed to cycloheximide and IGF I together showed super-induction of c-jun transcripts. Nuclear run-off assay revealed that IGF I up-regulated c-jun only while cyc...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(92)90513-k

    authors： Chiou ST,Chang WC

    更新日期：1992-03-16 00:00:00

  • The hemolytic activity of homocysteine is increased by the activated polymorphonuclear leukocytes.

    abstract：:Homocysteine is an accepted risk factor when its plasma level exceeds the physiological upper limit of 12 mumol/L. We found in vitro that homocysteine is able to lyse the erythrocytes (RBCs) at higher concentrations than 15 mumol/L only when activated polymorphonuclear leukocytes (PMNL) are present. The hemolytic effe...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.1449

    authors： Olinescu R,Kummerow FA,Handler B,Fleischer L

    更新日期：1996-09-24 00:00:00

  • Phenotypes of ATP-activated current associated with their genotypes of P2X1-6 subunits in neurons innervating tooth-pulp.

    abstract：:To explore the association of the phenotype of ATP-activated current with the genotype of P2X1-6 subunits in nociceptors, we developed a method that allows us to label nociceptive neurons innervating tooth-pulp in rat trigeminal ganglion (TG) neurons using a retrograde fluorescence-tracing method, to record ATP-activa...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.01.156

    authors： Liu Y,Tian X,Wu Y,Chen L,Yi CL,Li ZW,Zhang Y,Li CY

    更新日期：2015-03-13 00:00:00

  • Cardioprotective effects of recombinant human erythropoietin in rats with experimental autoimmune myocarditis.

    abstract：:Erythropoietin (EPO) has been known to have cytoprotective effects on several types of tissues, presumably through modulation of apoptosis and inflammation. The effect of EPO on myocardial inflammation, however, has not yet been clarified. We investigated the cardioprotective effects of EPO in rats with experimental a...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.03.230

    authors： Mitsuma W,Ito M,Kodama M,Fuse K,Okamura K,Minagawa S,Kato K,Hanawa H,Toba K,Nakazawa M,Aizawa Y

    更新日期：2006-06-09 00:00:00

  • Real-time monitoring of RNA helicase activity using fluorescence resonance energy transfer in vitro.

    abstract：:We have developed a continuous fluorescence assay based on fluorescence resonance energy transfer (FRET) for the monitoring of RNA helicase activity in vitro. The assay is tested using the hepatitis C virus (HCV) NS3 helicase as a model. We prepared a double-stranded RNA (dsRNA) substrate with a 5' fluorophore-labeled...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.01.100

    authors： Tani H,Fujita O,Furuta A,Matsuda Y,Miyata R,Akimitsu N,Tanaka J,Tsuneda S,Sekiguchi Y,Noda N

    更新日期：2010-02-26 00:00:00

  • Neuronal expression of a Caenorhabditis elegans elav-like gene and the effect of its ectopic expression.

    abstract：:We examined the expression of a Caenorhabditis elegans (C. elegans) elav-like gene, which we designated elr-1. The elr-1 gene encodes a predicted 456-amino-acid protein containing three putative RNA-binding domains and belongs to the ELAV family, which is functionally involved in neuronal differentiation. Northern blo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.0957

    authors： Fujita M,Kawano T,Ohta A,Sakamoto H

    更新日期：1999-07-14 00:00:00

  • Novel cancer chemopreventive effects of a flavonoid antioxidant silymarin: inhibition of mRNA expression of an endogenous tumor promoter TNF alpha.

    abstract：:In this study we describe exceptionally high protective effects of silymarin, a flavonoid antioxidant isolated from milk thistle, against 12-O-tetradecanoylphorbol 13-acetate (TPA)- and okadaic acid (OA)-caused tumor promotion in SENCAR mouse skin. Pre-application of silymarin to that of TPA in 7, 12-dimethylbenz(a)an...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.7375

    authors： Zi X,Mukhtar H,Agarwal R

    更新日期：1997-10-09 00:00:00

  • Next-generation sequencing-based analysis of reverse transcriptase fidelity.

    abstract：:In this study, we devised a simple and rapid method to analyze fidelity of reverse transcriptase (RT) using next-generation sequencing (NGS). The method comprises a cDNA synthesis reaction from standard RNA with a primer containing a tag of 14 randomized bases and the RT to be tested, PCR using high-fidelity DNA polym...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.07.169

    authors： Yasukawa K,Iida K,Okano H,Hidese R,Baba M,Yanagihara I,Kojima K,Takita T,Fujiwara S

    更新日期：2017-10-14 00:00:00

  • Wild-type and mutated nicastrins do not display aminopeptidase M- and B-like activities.

    abstract：:Nicastrin is a recently discovered protein interacting with presenilins and the beta-amyloid precursor protein, the proteins playing key roles in Alzheimer's disease and which, when mutated, appear responsible for early-onset familial forms of Alzheimer's disease. Nicastrin was reported to modulate beta-amyloid produc...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.6030

    authors： Fergani A,Yu G,St George-Hyslop P,Checler F

    更新日期：2001-12-07 00:00:00

  • Auxin-regulated changes in protein phosphorylation in pea epicotyls.

    abstract：:Auxins regulate various aspects of plant growth and development. However, the mechanism by which these hormones elicit diverse physiological processes is not clear. We present evidence for the role of auxin in protein phosphorylation and the possible involvement of calmodulin in auxin-induced changes. In the presence ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(87)80055-4

    authors： Reddy AS,Chengappa S,Poovaiah BW

    更新日期：1987-04-29 00:00:00

  • Inhibitory effect of memantine, an NMDA-receptor antagonist, on electroporation-induced inward currents in pituitary GH3 cells.

    abstract：:The membrane electroporation-induced inward current (IMEP) in pituitary tumor (GH3) cells was characterized. This current emerges irregularly when membrane hyperpolarizations to -200 mV with a holding potential of -80 mV were elicited. Neither E-4031 (10 μM), glibenclamide (30 μM), nor ZD7288 (30 μM) caused any effect...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.01.066

    authors： Wu SN,Huang HC,Yeh CC,Yang WH,Lo YC

    更新日期：2011-02-18 00:00:00

  • Lactosylceramide synthase β-1,4-GalT-V: A novel target for the diagnosis and therapy of human colorectal cancer.

    abstract：:Little is known about an oncogenic signal transducer β-1,4-galactosyltransferase-V (β-1,4-GalT-V), in human colorectal cancer. Using quantitative RT-PCR, immunohistochemical staining and ELISA assays, we determined that β-1,4-GalT-V gene/protein expression is specifically increased in human colorectal cancer (CRC) tum...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.11.149

    authors： Chatterjee SB,Hou J,Bandaru VVR,Pezhouh MK,Syed Rifat Mannan AA,Sharma R

    更新日期：2019-01-08 00:00:00

  • Targeting of renal proximal tubule Na,K-ATPase by salt-inducible kinase.

    abstract：:The renal proximal tubule (RPT) is a central locale for Na+ reabsorption, and blood pressure regulation. Na+ reabsorption in the RPT depends upon the Na,K-ATPase, which is controlled by a complex regulatory network, including Salt-Inducible Protein Kinase (SIK). SIKs are recently discovered members of the AMP-activate...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章,评审

    doi：10.1016/j.bbrc.2010.02.037

    authors： Taub M,Springate JE,Cutuli F

    更新日期：2010-03-12 00:00:00

  • Calcium-activated, calmodulin-dependent protein kinase activity in bovine thyroid cytosol.

    abstract：:Bovine thyroid 100,000 X g supernatant contained calcium-activated, calmodulin-dependent protein kinase (PK-CaM) activity. The PK-CaM was partially purified using ion-exchange chromatography and characterized. PK-CaM, using casein as exogenous substrate, was not stimulated by Ca2+(0-500 microM) or calmodulin (1-10 mic...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(86)91066-1

    authors： Friedman Y,Henricks L,Poleck T,Levasseur S,Burke G

    更新日期：1986-10-15 00:00:00

  • TGFβ1-induced down-regulation of microRNA-138 contributes to epithelial-mesenchymal transition in primary lung cancer cells.

    abstract：:The existence of cancer stem cells within the tumor could lead to cancer therapy resistance. TGFβ1 is considered as one of the most powerful players in the generation of CSCs through induction of epithelial-mesenchymal transition in different types of cancer including lung cancer, however, the detailed mechanisms by w...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.01.164

    authors： Zhang F,Li T,Han L,Qin P,Wu Z,Xu B,Gao Q,Song Y

    更新日期：2018-02-19 00:00:00